DEPIK Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan
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Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments
Get PDFMangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin colum
The correlation between environmental parameters and the abundance of crabs in the mangrove ecosystem of Gemuruh River, Koto XI Tarusan District, West Sumatera
Get PDFCrabs are a member of the mangrove ecosystem fauna and get their food source from litter. They turn the litter into detritus and maintain ecological balance. In addition, environmental conditions in the mangrove ecosystem also play an essential role in the abundance of brachyuran crabs. Influential environmental factors include temperature, salinity, water pH, and substrate type. This study aims to determine the effect of environment types on the presence of brachyuran crabs from families Ocypodidae and Sesarmidae in the mangrove ecosystem in Gemuruh River, Koto XI Tarusan District. The method used is a descriptive method, which is a direct observation approach to crabs and environmental conditions in the mangrove ecosystem area. Collection of crab samples and environmental condition data used a purposive sampling technique. The result shows six species distributed in the area, namely Uca bellator, U. rosea, Perisesarma eumolpe, Sarmatium germaini, P. plicatum, and Sesarma curoense. The distribution of species crabs in vegetation mangroves is Rhizophora stylosa, R. apiculata, R. mucronata, Sonneratia alba, and Nypa fruticans. The environmental parameters (temperature, salinity, water pH) at each station were within the relatively good category for the growth of the Ocypodidae and Sesarmidae crab families. Based on the analysis results of the relationship between the abundance of brachyuran crabs and environmental parameters (temperature, salinity, water pH), it can be seen that there is no significant correlation between temperature, salinity, and water pH with the abundance of Ocypodidae and Sesarmidae brachyuran crabs families in the mangrove ecosystem of Gemuruh River. The factor with a dominant influence on the abundance of these crabs is the substrate type.Keywords:AbundanceKoto XI TarusanMangroveOcypodidaeSesarmida
Oceanographic studies as the basis for shipyard development at the Fish Landing Port of Sabang
Get PDFShipbuilding is one of the marine industries which in development needs to pay attention to various parameters from the sea and land. This research was conducted in March to May 2022 with the aim of identifying the influence of oceanographic factors and water quality on the development of the shipbuilding industry at the fishing port of Sabang City. The oceanographic data used includes tides, wind, bathymetry, and sediment composition observed at 12 station points. The depth and tide data are then processed using QGIS. Wind data was analyzed using the Wind Rose Plot and sediment using the Wentworth scale. Oceanographic factors in Sabang Bay have shown very satisfactory results for the development of fish landing bases. The wind in the waters of Sabang Bay blows northwest with high speeds ranging from 10.00 m/s. The depth of the waters indicates a potential location for ship mooring. Sandy sediment is the most common type of sediment found. The tides that occur in the bay of Sabang are relatively stable. Oceanographic factors in Sabang Bay have shown very satisfactory results for the development of fish landing bases.Keywords:OceanographyShipyardSabang Ba
Important value index (IVI) and diversity of mangrove vegetation in Aceh Tamiang, Aceh Province
Get PDFMangrove ecosystem is one of the unique ecosystems that has high productivity and it grows along the coast of Aceh Province, Indonesia. The mangrove vegetation in Aceh Tamiang District was decreasing due to human exploitation i.e. stems of mangrove vegetation utilized by local communities become charcoal and firewood. These activities have a negative impact on live and growth of mangrove vegetation. Therefore, the purposes of the study are to analyses the important value index (IVI), mangrove species diversity, and to analyses the correlation between substrate characteristics with mangrove density. The research stations determined using the purposive sampling method and each station has three plots as replication. There were 4 research stations located in Seruway, Bandahara, Banda Mulia, and Manyak Paye sub districts. The study reported 12 species of mangrove in the trees category, 9 species of saplings, and 8 species of seedlings. The 12 species of mangroves found were Avicennia lanata, A. marina, A. officinalis, Brugeira parviflora, B. sexangula, Ceriops decandra, C. tegal, Lumnitzera recomosa, Nypa fruticans, Rhizopora apiculata, R. stylosa, and Sonneratia alba. The R. apiculata was species commonly found in each sampling location and it had highest IVI for the tree, sapling and seedling categories with the value of 207.24, 300, and 200, respectively. The average of diversity index was 1.56 categorized in the medium category. The moment Pearson correlation showed there was no significant correlation between the physical-chemical water parameters and substrate with the density of mangrove where Psig0.05.Keywords:Mangrove vegetationImportant value indexDensityDiversityAceh Tamian
Effects of difference C/N ratio on water quality of white leg shrimp (Litopenaeus vannamei) cultivation
Get PDFBiotechnology is used to apply technology to the C/N ratio by stimulating the activities of heterotrophic microorganisms, which derive food from carbon and nitrogen substrates in a certain ratio. The goal of this research was to see how the optimal carbon-nitrogen ratio affected water quality and floc volume in white leg shrimp (Litopenaeus vannamei) farming. This study took place in the UPT Integrated Laboratory Unkhair in Ternate City in July and August 2021. A fully randomized design (CRD) with four treatments and three replications was used in this investigation. Different C/N ratios had an effect on ammonia, nitrite, nitrate, floc volume, and feed conversion ratio, according to the research. The water quality parameters are still suitable for white leg shrimp cultivation. In white leg shrimp culture, the addition of C-organic molasses (different C/N ratios) was able to reduce the concentrations of (NH3), (NO2), and (NO3), and also greatly increase the flock volume and streamline the feed conversion ratio.Keywords:C/N RatioVannameiWastewaterBioflo
The clove oil effect on faunting time and survival rate of Oreochromis niloticus
Get PDFTo overcome excess residues and fish mortality rates during transportation, natural ingredients, namely clove oil, can be used. Compounds in clove oil are considered safe because they use natural ingredients. The purpose of this study was to determine the effectiveness of using clove oil as an anesthetic agent with different doses on the length of time of unconsciousness and survival rate of tilapia. This research was conducted from May to June 2023. This research was carried out at the Bungus Fish Seed Center, Padang, and West Sumatera. The method used in this study is the experimental method. The design used in this study was a completely randomized design (CRD) consisting of 4 treatments and 3 replications. The treatment used in this study is use of clove oil with different doses. Treatment A1 = 0.5 ml L-1 water, A2 = 1 ml L-1 water, A3 = 1.5 ml L-1 water and A4 = 2 ml L-1 water. Observations of changes in tilapia behavior and water quality were analyzed descriptively, while the length of time of stupor and survival of tilapia obtained during the study were analyzed using analysis of variance (ANOVA). To see the differences between treatments, the Duncant Multiple Range Test (DMNRT) was performed. Data is processed using the SPSS 26 program. The conclusion that can be considered in this study is that the administration of clove oil with different doses as an anesthetic has a significant effect on the longest induction time, on the length of time the fish were unconscious, the length of time the fish regained consciousness and survival rate of tilapia. In treatment A2 with a dose of 1 mL L-1 of water, it was optimal to stun tilapia for 244 minutes with a survival of 88.89%.Keywords:Clove oilFish transportationTilapi
Model of nitrogen-phosphorus ratio and phytoplankton relationship in lake Laut Tawar, Indonesia
Get PDFPhytoplankton blooms in the lake cause ecological, economic, health, energy, and aesthetic losses. It reduces water quality and biota diversity, creates toxins in the waters, and changes the structures and functions of the ecosystem. The essential nutrients for the growth of phytoplankton are nitrogen and phosphorus. Controlling phytoplankton growth can be managed by controlling the limiting nutrient input. This study aims to identify the limiting nutrient, analyze variations in TN:TP ratio spatially and temporally, and model TN:TP ratio and chlorophyll-a relationship. This study used secondary data from previous studies, namely TN, TP, and chlorophyll-a observed monthly in seven stations purposively during a year. Rainfall data was also obtained from the previous study. Limiting nutrients were determined by Redfield theory, and data were analyzed by Spearman correlation, One-way ANOVA, Kruskal-Wallis, and regression analysis. The results showed phosphorus was a limiting nutrient for phytoplankton growth in Lake Laut Tawar. TN:TP ratio and chlorophyll-a did not vary spatially, indicating the lake surface waters were evenly mixed. The parameters varied temporally, expressing the influence of hydroclimatological factors, especially rainfall. Rainfall increases nutrient input to the lake, but only rain below 200 mm/month causes an increase in the concentration of nutrients in the lake. The rainfall above 200 mm/month increases lake water volume significantly, thereby reducing nutrient concentrations. TN:TP ratio and chlorophyll-a related negatively and formed a non-linear relationship with an empirical model Chlorophyll-a = 2770.285 (TN/TP)-1.871. Eutrophication of Lake Laut Tawar should be anticipated by controlling the anthropogenic phosphorus input.Keywords:AnthropogenicChlorophyll-aEutrophication,Limiting nutrientRainfal
Molecular characterization of Tapah fish (Wallago leerii, Bleeker 1851) from Riau Province based on Cytochrome b gen
Get PDFTapah fish (Wallago leerii) is a fish with high economic value in Riau Province. Molecular research on W. leerii is very important to do. This study aims to determine the molecular characteristics of W. leerii from Riau Province based on the Cytochrome b gene. The universal primer Cytochrome b is used for the Polymerase Chain Reaction (PCR) process. The PCR result is a partial Cytochrome b gene with a length of 373 bp. Multiple alignments were performed on nucleotide sequences of the Cytochrome b gene of W. leerii from river of Tapung and Indragiri Riau with the cytochrome b gene of other fish from Genbank and analyzed using the MEGA program version 6.0. Phylogenetic construction using the Cytochrome b gene can distinguish W. leerii from river of Tapung and Indragiri Riau, with other species.Keywords:AlignmentsMolecularGenBan