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Successful cryostorage of twenty authenticated cell lines derived from eight species of marine fish maintained over a period of more than five years
No full textSuccessful cryostorage of twenty authenticated cell lines derived from eight species of marine fish maintained over a period of more than five year
Humoral antibody response and protective immunity in the grouper, Epinephilus malabaricus (Bloch and Schneider) immunised with whole cell killed vaccine preparation from a virulent starin of Vibrio anguillarum)
No full textHumoral antibody response and protective immunity in the grouper, Epinephilus malabaricus (Bloch and Schneider) immunised with whole cell killed vaccine preparation from a virulent starin of Vibrio anguillarum
Development of a Cell Culture System from Gill Explants of the Grouper, Epinephelus malabaricus (Bloch and Shneider)
Full text linkA cell culture system was developed successfully from gill explants of the Malabar grouper, Epinephelus malabaricus. Gill tissue samples aseptically excised from healthy juveniles of E. malabaricus were explanted in Leibovitz’ L-15 medium supplemented with 0.07 M NaCl and 20% fetal bovine serum (FBS). A mixture of different types of cells emerged from the explants, and these cells were observed to spread and attach to the culture flasks from the second day onwards. Confluent monolayers comprising epithelioid as well as fibroblast-like cells were formed within ten days. The cells were found to grow well at 28 + 2°C. The cell monolayers were subcultured by trypsinization and seeded into new flasks, which produced confluent monolayers comprising predominantly epithelioid-like cells in subsequent passages
Biology and fishery of flatfishes
Full text linkFlatfishes of the order Hetrosomata or Pleuronectiformes,
comprising of the halibuts, flounders and soles occupy a place of
importance among the marine fishery resources of India. Although
Norman has described 91 species, only few species actually contribute to
the fishery. Every year around 44836 tonnes of flatfish are being landed
on an all - lndia basis. The major contributor to the all lndia landings is
Kerala (39 %) followed by Gujarat (17 %) and closely by Karnataka (14.95
%