Jurnal Sain Veteriner
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CONSTRUCTION OF AN EXPRESSION VECTOR BASED ON pCDNA3.1(+) EXPRESSING JEMBRANA DISEASE VRIUS ENV-TM SUBUNIT GENE IN EUKARYOTIC SYSTEM
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STUDI EKSPERIMENTAL SISTA JARINGAN Toxoplasma gondii SECARA IN VIVO AN EXPERIMENTAL STUDY OF Toxoplasma gondii TISSUE CYST IN VIVO
No full textSTUDI GAMBARAN DARAH BURUNG ELANG YANG DIPELIHARA DI KEBUN BINATANG GEMBIRA LOKA YOGYAKARTA
No full textThe Normal of Peristalistic Waves In Vitro In The Small Intestine of Local Rabbits
Get PDFlocal male rabbits aged 4 months, 1.0 to 1.5 kg body weight and healthy adaptation in individual cages with food and water ad libitum. All of rabbits were randomly divided into 2 groups: groups I (K-1) and II (K-2) eachof 4 head / group. All of rabbits fasted for 12 hours, and anesthetized with urethane 1.55 g/ kg bw in a 25% solution intraperitoneally. Each rabbit dissected, segments of small intestine (duodenum, jejunum, ileum) weretaken each 1.5 cm for the measured peristaltic wave. The K-1 group of rabbits were measured peristaltic wave using Physiograph and the K-2 with Kymograph. Physiograph or Kymograf operated after the specified time of 1 second and the impression of speed 10 mm/detik paper on the sensitivity of 0.1 and the sensitivity of the amplifier 50. During the calibration operation is often performed. Data obtained by the peristaltic waves (frequency and amplitude of contractions) small intestine for 5 minutes were analyzed by t test. The results of measurements of intestinal peristalsis by using Physiograph or Kymograf showed that the frequency and amplitude of peristaltic contractions of the proximal part is higher and more caudal direction tends to go down. Therefore it was concluded that the normal peristaltic waves of the small intestine to more caudal were getting less and less interm of the frequency and amplitude of peristaltic contractions.Keywords: rabbits,small intestine, peristaltic, physiograph, kymograp
Distribusi dan Identifikasi Spesies-Spesies Calicoides (Deptera: Ceratopogonidae) di Kabupaten Bogor
No full textTelah diteliti distribusi dan identifikasi spesies-spesies Culicoides yang ada di sekitar kandang temak sapi di Kabupaten Bogor. Penelitian ini ditujukan untuk mengetahui spesiesspesies Culicoides yang mempunyai peranan dalam penyebaran penyakit bluetongue pada ternak. Sebanyak 2117 ekor Culicoides (Diptem: Ceratopogonidae) dikumpulkan dari kandang ternak sapi di wilayah Depok dan Cibungbulang, Kabupaten Bogor dengan menggunakan perangkap serangga Pirbright-type miniatur light trap. Identifikasi spesies dilakukan berdasarkan karakter morfologi menurut Wirth dan Hubert. Hasil penelitian menunjukkan bahwa Culicoides lebih banyak ditemukan di wilayah Depok daripada di Cibungbulang. Ada empat belas spesies dari kedua lokasi tersebut yang berhasil diidentifikasi. Spesies Culicoides yang dominan di daerah Depok adalah C. parahumeralis Wirth & Hubert, C. acioni Smith, C. ozystoma Kieffer dan C peregrinus Kieffer; sedangkan spesies yang dominan di Cibungbulang adalah C. parahumeralis, C. orientalis, C. orientalis Macfiei, C. peregrinus dan C. caystoma. Spesies Culicoides yang dicurigai sebagai penyebar penyakit bluetongue adalah: C. actoni, C. oxystoma, C. peregrinus dan. C. orientali
THE EFFECT OF HIGH LIPID DIET LIPOPOLYSACCHARIDE AND LAPARATOMY SURGERY ON THE LEVEL OF C-REACTIVE PROTEIN SERUM IN SPRAGUE DAWLEY RATS EKD
Get PDFC-reactive protein (CRP) is a member of the family of proteins known as pentraxins, and originally defined as a substance, observed in the serum or plasma of patient with acute infections, that reacted with the Cpolysaccharide of the pneumococcus. Serum concentrations of CRP increase as part of the inflammatory response to infection or acute injury. The aim of the study was to see the differences between acute and chronicstimuli in the increasing of CRP concentration in serum of Sprague Dawley rat. Twenty male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted for 5 days and given basal diet,containing normal fat(5% of fat) and water ad libitum. Rats were then divided into 4 groups of 5 each. Group 1 was used as control animal. The animals in this group were fed basal diet for 59 days without any othertreatments. Group 2 was animals fed high fat diet containin g20Yo of fat, for 59 days without any other treatments. Group 3 was animals fed basal diet for 59 days and in the day of 60 then injected with LPS from Escherichia coli 0111:B4, 1 mg/kg BW intraperitoneally, and Group 4 was animals fed basal diet for 59 days and in the day of 60 animals then laparatomized. Twenty four hours after all treatments, blood sample were collected from orbitally vein, serum was separated and used to analyze CRP concentration. Statistical analyzes using onew ay analyzeso fvariances howed that there were significance differences among Group 1,2,3 and 4 (p<0.05). Mean of CRP concentrations respectively from the highest to the lowest were: Group 3, Group 4, Group 2 and Group 1. Fromthe result of the study it can be concluded that CRP concentration of acute stimulus was higher than chronic stimulus.Key words : C-reactivep rotein, lipopolysaccharides, high fat diet, laparatomy