Jurnal Sain Veteriner
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Bovine Viral Diarrhea Virus Antigen and Immunoglobulin-G Detection in Unvaccinated Cattle
Full text linkTo address the community's nutritional for meat can be supported by importing cattle while maintaining vigilance against Bovine Viral Diarrhea (BVD), an infectious disease caused by the Bovine Viral Diarrhea Virus (BVDV). This pathogen is associated with gastrointestinal, respiratory, and reproductive issues in cattle worldwide. Consequently, controlling BVDV stands as a paramount measure in preventing BVD. One effective strategy in this endeavor is vaccination. The successful implementation of vaccination relies on acquiring comprehensive information about BVDV antigens and antibodies present in the serum of unvaccinated cattle, which can provide insights into the protein profile resulting from natural infection. This study aimed to detect BVDV antigen and immunoglobulin-G (IgG) based on molecular weight in protein profiles from various cattle breeds imported into Indonesia. Utilizing the SDS-PAGE method, the analysis results revealed protein bands at 55.3 kDa and 151.3 kDa were detected in the serum of BVD-positive cows, meanwhile, these specific profiles were absent in BVD-negative cows. It can be concluded that the 55.3 kDa represents a BVDV antigen, while the 151.3 kDa corresponds to an IgG antibody profile. These findings can serve as one of the basic foundations for developing a BVD vaccine for cattle populations
Economic Impact of Foot and Mouth Disease in Dairy Farmers at Koperasi Peternakan Bandung Selatan (KPBS) Pangalengan, Indonesia
No full textThis study aims to analyze the economic impact of the foot and mouth disease (FMD) outbreak on dairy farmers who are members of the Pangalengan South Bandung Livestock Cooperative (KPBS). Data were collected through structured questionnaires, in-depth interviews, and field observations of 100 FMD-affected farmers during September-November 2023, with follow-up evaluation one year after the outbreak. The results showed that during the outbreak (29 days), the average loss per farmer reached IDR 44,018,068 or IDR 6,288,295 per head, mostly due to decreased milk production, dairy cow mortality, and disease control costs, including treatment, vaccination, and disinfection. One year after the outbreak (365 days), economic losses were IDR 46,077,090 or IDR 6,582,441 per head, dominated by the residual impact of FMD in the form of a 39.75% reduction in milk yield (18.31 kg/day). Economic losses also involved additional costs, such as vector control and livestock burial, which further burdened farmers. These findings highlight the importance of more effective FMD control policies, including mass vaccination, improved biosecurity and financial assistance for affected farmers, to reduce economic impacts and support long-term recovery
Koleksi Semen pada Burung Parkit: Teknik Non-Invasif Pemijatan Kloaka
No full textKoleksi semen merupakan ejakulasi yang dipengaruhi oleh faktor eksternal seperti lingkungan, perlakuan, dan alat koleksi (Madeddu et al., 2022; Vitorino Carvalho, 2024). Koleksi semen pada burung parkit dapat dilakukan dengan pemijatan kloaka maupun elektroejakulator (Lierz et al., 2013; Kucera & Heidinger, 2018; Frediani et al., 2019). Koleksi semen dengan elektroejakulator direkomendasikan untuk burung berukuran besar seperti burung bangau dan kalkun dengan tingkat keberhasilan sebesar 67%, sementara pemijatan kloaka direkomendasikan untuk burung kecil seperti parkit dengan tingkat keberhasilan 74
Variasi Karakter Resistensi Antibiotik Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Ettawa di Yogyakarta
No full textSalah satu penyakit yang sering dijumpai dalam budidaya kambing Peranakan Etawa (PE) adalah mastitis. Beberapa bakteri patogen dapat menyebabkan mastitis diantaranya adalah Staphylococcus sp., E. coli, Klebsiella spp., dan Streptococcus spp. Meningkatnya kejadian penyakit menyebabkan meningkatnya penggunaan antibiotik yang berakibat pada peningkatan potensi resistensi dan residu antibiotik. Penelitian ini bertujuan untuk mengetahui variasi karakter fenotipik resistensi bakteri penyebab mastitis yang diisolasi dari susu kambing PE yang mengalami mastitis subklinis terhadap berbagai golongan antibiotik yang umum dipakai petugas medis veteriner di wilayah Yogyakarta. Sebanyak 8 isolat diperoleh dari peternakan rakyat di wilayah kecamatan Kokap, Kulon Progo,, sedangkan 4 isolat diperoleh dari wilayah Pakem, Sleman, Yogyakarta. Dua belas isolat tersebut terdiri dari 3 jenis bakteri yang berbeda yaitu Staphylococcus sp., Escherichia coli, dan Klebsiella sp. Berdasarkan hasil uji antibiogram diketahui bahwa terdapat perbedaan pola resistensi antibiotik antara isolat bakteri penyebab mastitis dari Kulon Progo dan Sleman. Ampicillin, penicillin G, tetracyclin, dan cefoxitin efektif digunakan untuk pengobatan mastitis di daerah Sleman, sedangkan di Kulon Progo gentamicyn, tetracyclin dan erythromycin adalah pilihan antibiotik yang efektif digunaka
Anthelmintic Potential of Annona muricata seed Infusion: An In Vitro Study on Haemonchus contortus in Goats
Full text linkParasitic infections, particularly those caused by Haemonchus contortus, significantly impact the health and productivity of small ruminants worldwide. Traditional anthelmintic treatments are increasingly challenged by resistance issues, prompting the search for alternative treatments. The objective of this study was to assess the effectiveness of Annona muricata seed infusion on Haemonchus contortus mortality in goats in vitro. The research was conducted from November 2023 to March 2024 at the Laboratory of the Faculty of Agriculture, Islamic University of Kadiri, and BRIN. The method employed was one-way ANOVA. Treatments included a negative control with 0.9% NaCl, 10% concentration infusion, 20% concentration infusion, and albendazole. Mortality rates were assessed at 0.5, 1, 2, 4, 6, 8, 10, and 12-hour intervals. The results revealed that Annona muricata seed infusion contains compounds such as tannins, flavonoids, alkaloids, saponins, and steroids, which are capable of damaging the cuticle and buccal tissues of Haemonchus contortus. Phenolic content was measured at total phenolics of 9.1 mg RE / g Dw, total flavonoids of 4.7 mg RE / g Dw, total tannins of 7.2%, condensed tannins / CT of 3.4%, and hydrolyzable tannins / HT of 3.9%. Infusions at 10% and 20% concentrations achieved 100% mortality of Haemonchus contortus by the 8th hour. Scanning Electron Microscopy (SEM) revealed structural changes in Haemonchus contortus at different infusion concentrations, including wrinkling, irregular lines, and the presence of holes in the cuticle and buccal regions. The infusion of Annona muricata seed infusion is effective as an anthelmintic against Haemonchus contortus, achieving 100% mortality at concentrations of 10% and 20% within 8 hours. The study also demonstrates structural damage to the worms, affirming the potential of Pineapple Peel infusion as a natural parasitic control in small ruminants
Antibacterial Activity of Turmeric (Curcuma domestica Val.) Extract on the Growth of Klebsiella sp.: In Vitro
Full text linkKlebsiella sp. is one of the causes of bacterial diseases that attack large farm animals and poultry. Treatment of infections caused by these bacteria is using antibiotics. Cases of antibiotic resistance against Klebsiella sp. encouraging researchers to study spice plants as antibacterial, especially turmeric (Curcuma domestica Val.). Research related to the antibacterial activity of turmeric against Klebsiella spp. not much has been done. Therefore, this study aimed to determine the antibacterial activity of turmeric extract against Klebsiella sp. in vitro. re-identified by testing on MacConkey agar, triple sugar iron agar, and IMViC (indole, methyl red, Voges-Proskauer, citrate). Overall results showed the characteristics of Klebsiella sp. Making turmeric powder simplicia extract by maceration method followed by evaporation using a spray dryer. Turmeric extract was diluted with dimethyl sulfoxide (DMSO). The antibacterial activity test of turmeric extract (20%, 40%, 60%, 80%, 100%) was carried out on Mueller-Hinton agar that had been given wells. Chloramphenicol (30 g) was used as a positive control and DMSO as a negative control. The test was carried out with three repetitions. The mean inhibition zone diameter of three repetitions was 20.00 mm; 0.00mm; 10.17mm; 10.67 mm; 10.67 mm; 11.17 mm and 12.17 mm for positive control, negative control, 20-100% turmeric extract. Based on the results of phytochemical tests, turmeric extract contains flavonoids, alkaloids, quinones and triterpenoids. Turmeric extract with concentrations of 20%, 40%, 60%, 80% and 100% had the activity to inhibit the growth of Klebsiella sp. with a strong category on in vitro testing
Acute Anti-Inflammatory Study by Oral Administration of Cherry Leaf (Muntingia Calabura L.) Infusion in Inflammation-induced Wistar Strain White Rats (Rattus Norvegicus)
No full textInflammation is a response of the body’s defense system in preparation for tissue repair. Acute inflammation is characterized by heat, redness, pain, and formation of edema. Acute inflammation involve the realese of inflammatory mediators. Muntingia calabura is a herbal plant commonly used as an anti-inflammatory medicine containing bioactive coumpounds such as flavonoids, triterpenoids, and alkaloids that can inhibit the regulation of inflammatory mediators. This study aims to determine the anti-inflammatory activity and optimal dosage of M. calabura infusion in addressing acute inflammation in rats. The research method is a laboratory experiment using Wistar strain white rats that divided into 7 treatment groups: normal, negative control, positive control (sodium diclofenac and methylprednisolone), and M. calabura infusion at doses of 150, 300, and 600 mg/kg body weight. Each rats model induced with 1% carrageenan at 0.2 ml intraplantar, the formed edema is measured using a plethysmometer every 30 minutes for the first 3 hour and every 60 minutes for the following 3 hour. The result indicate that M. calabura infusion has acute anti-inflammatory effect on white rats model induced with carrageenan, with the most optimal dose being 600 mg/kg body weight, showing a pattern of anti-inflammatory activity similar to methylprednisolone
ANGKA KEJADIAN DAN KARAKTERISTIK LESI PATOLOGI CALICIVIRUS PADA KUCING (Felis catus) DI VEE VET BANDUNG PERIODE AGUSTUS-SEPTEMBER 2024
Full text linkFeline calicivirus merupakan penyakit akibat virus yang sangat menular pada kucing. Penyakit ini menyerang saluran pernapasan bagian atas. Feline calicivirus memiliki angka morbiditas yang tinggi dengan gejala yang umumnya serupa dengan penyakit lain. Penelitian ini bertujuan untuk mengetahui angka kejadian serta karakteristik lesi patologi feline calicivirus pada kucing di Vee Vet Bandung periode Agustus – September 2024. Metode penelitian ini dilakukan dengan mengambil data langsung kepada seluruh kucing yang datang ke Vee Vet Bandung periode Agustus-September 2024 dengan keluhan lesi pada mulut dan gangguan pernapasan bagian atas. Pengambilan data dilakukan dengan melakukan uji rapid test dan mendokumentasikan lesi untuk mengetahui bentuk serta lokasi lesi pada kucing dengan kriteria yaitu, hasil positif rapid test feline calicivirus, terdapat lesi pada mulut, dan terdapat gangguan pernapasan bagian atas. Selanjutnya, data akan diolah pada microsoft excel yang kemudian akan dibahas secara deskriptif. Hasil penelitian menunjukan bahwa angka kejadian dari feline calicivirus di Vee Vet Bandung periode Agustus-September 2024 tergolong sangat rendah yaitu 0.05%. Hasil tersebut didapatkan dari 4 pasien terdiagnosa feline calicivirus dengan seluruh populasi kucing yang datang sebanyak 722 pasien. Karaktersitik lesi patologi yang muncul pada seluruh kucing terdiagnosis feline calicivirus adalah seluruh kucing memiliki lesi pada mulut berupa ulser kemerahan khususnya pada bagian lidah dan mukosa mulut serta tidak terjadi nekrosis ataupun edema baik itu di wajah atau pawpad.
DESAIN PRIMER BERDASARKAN GEN MT-12S rRNA UNTUK MENDETEKSI CEMARAN DAGING BABI PADA PRODUK OLAHAN ASAL DAGING SAPI DENGAN METODE MULTIPLEKS-PCR
No full textThe case of adulteration of pork in beef products, which causes fretfulness especially for muslims, is still found in Indonesia. Multiplex Polymerase Chain Reaction (Multiplex PCR) can help detecting the adulteration of pork in beef products. This method needs multiple specific primers to amplify several targeted DNA mixture in single step reaction. This study aimed to design specific primers that can be utilized to detect the adulteration of pork in beef products using Multiplex PCR method.Firstly, primers were designed in silico using bioinformatic tools which were MegAlign, Seqbuilder, and AmplifX. This design was based on mt-12s rRNA gene. The length of amplified fragment using the designed primers for cow was 91 bp, while the pig was 230 bp. Secondly, the designed primers were tested to amplify DNA of cow and pig from raw beef, pork, mixture of beef and pork, and twelve samples of their products (meatballs) to assess their ability to amplify targeted DNA spesifically.The result showed that the designed primers could amplify the targeted DNA specifically from raw beef, pork, mixture of both meat, and their products. Therefore, the designed primers evidently can be used to detect adulteration of pork in beef by Multiplex PCR method
Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity
No full textToxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands