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Identifikasi Gen Transgenik pada Produk Susu Bubuk Kedelai dan Susu Formula Soya dengan Metode PCR (Polymerase Chain Reaction)
Get PDFThe need of soybean that reached up to 2,2 million tons per year has made Indonesia imports this commodity 1,62 million tons from countries that adopt Genetically Modified (GM) soybean. By the presence of GM soybeans in Indonesia, Genetically Modified Product (GMP) labelling has need to be done. Detection of GMP can be done by using PCR. The aim of this study was to determine the presence of trangenic genetic material in soy milk powder and soy formula products to classify as GMP. Another goal was to determine the optimum annealing temperature of the primers used. Based on this study, the optimum annealing temperature of the CaMV 35S primer and the EPSPS-CP4 primer was 60oC and 59oC. The NOS terminator primer’s optimum annealing temperature was not found. 6 soy milk powder samples and 5 soy formula samples are might be determined to be using transgenic soybeans due to the presence of EPSPS-CP4 genes and CaMV 35S promotor genes. Therefore, those 11 samples were classified as GMP.ABSTRAK Kebutuhan kedelai yang mencapai 2,2 juta ton/tahun memaksa Indonesia mengimpor sebanyak 1,62 juta ton. Sebagian besar kedelai impor berupa kedelai transgenik. Dengan munculnya kedelai transgenik di Indonesia, perlu adanya pelabelan Produk Rekayasa Genetika (PRG) untuk memenuhi hak-hak konsumen. Teknik yang dilakukan untuk mendeteksi PRG salah satunya menggunakan metode PCR. Penelitian ini bertujuan untuk mengetahui ada tidaknya gen transgenik pada produk susu bubuk kedelai dan formula soya, sehingga produk dapat digolongkan sebagai PRG atau tidak. Selain itu juga bertujuan untuk mengetahui suhu annealing optimum pada primer yang digunakan. Hasil penelitian didapatkan suhu annealing optimum primer CaMV 35S promotor adalah 60oC. Sedangkan untuk primer gen EPSPS-CP4 suhu annealing optimumnya 59oC. Untuk primer NOS terminator suhu annealing optimum tidak ditemukan. Dari amplifikasi DNA sampel, 6 sampel susu bubuk kedelai dan 5 sampel formula soya terdapat sisipan gen EPSPS-CP4 dan gen Promotor CaMV 35S. Dengan demikian 11 sampel tersebut dapat dikatakan sebagai PRG. Kata kunci: Produk rekayasa genetika; PCR; formula soya; susu kedelai bubuk; kedelai transgeni
Formulation and Characterization of Phycocyanin Microcapsules within Maltodextrin-Alginate
Get PDFFikosianin merupakan sumber pewarna biru alami yang dapat diekstrak dari Spirulina sp. Karakteristik dari fikosianin adalah tidak stabil oleh cahaya, suhu, dan pH selama proses pengolahan dan penyimpanan. Metode mikroenkapsulasi dapat digunakan untuk melindungi fikosianin dari pengaruh eksternal di mana jenis dan konsentrasi enkapsulan yang digunakan dapat mempengaruhi karakteristik mikrokapsul fikosianin yang dihasilkan. Tujuan dari penelitian ini adalah untuk mengetahui formulasi terbaik dan karakterisasi mikrokapsul fikosianin dari Spirulina sp. dengan maltodekstrin dan alginat sebagai enkapsulan. Mikrokapsul diproduksi dengan lima perbedaan konsentrasi alginat dalam maltodekstrin, yaitu 0 %; 0,2 %; 0,4 %; 0,6 %; dan 0,8 % (b/b). Total enkapsulan yang digunakan adalah 10 % dari larutan mikropartikel fikosianin. Hasil penelitian menunjukkan bahwa peningkatan konsentrasi alginat dapat meningkatkan kadar fikosianin, kadar air, efisiensi enkapsulasi, bulk density, intensitas warna biru, dan ukuran partikel serta dapat memperbaiki morfologi mikrokapsul yang dihasilkan. Mikrokapsul fikosianin dengan konsentrasi alginat 0,6 % dan maltodekstrin 9,4 % memiliki kadar fikosianin, efisiensi enkapsulasi, dan intensitas warna biru paling tinggi.Phycocyanin is a source of natural blue dye which can be extracted from Spirulina sp. The main characteristics of phycocyanin are unstable by temperature and pH during processing and storage. Microencapsulation methods could be proposed to protect the phycocyanin from the external effect, where the types and concentration of encapsulant used may affect the characteristics of the result. The aim of this study was to determine the best formulation and characterization of phycocyanin microcapsules from Spirulina sp. with maltodextrin and alginate as an encapsulant. The microcapsules were produced using five different concentrations of alginate in maltodextrin, namely 0%; 0.2%; 0.4%; 0.6%; and 0.8% (w/w). The total encapsulant used was 10% of phycocyanin microparticle solution. The results showed that the increasing concentration of alginate could raise the levels of phycocyanin, moisture content, encapsulation efficiency, bulk density, blue intensity, and particle size, it also improved the morphology of the microcapsules. Phycocyanin microcapsules with alginate concentration of 0.6% and 9.4% maltodextrin had the highest phycocyanin content, encapsulation efficiency, and blue intensity
Kinetika Perubahan Mutu Minyak Buah Merah (Pandanus conoideus) Selama Penyimpanan
Get PDFRed fruit (Pandanus conoideus) oil contains high unsaturated fatty acids that are readily oxidized during storage, consequently, this affects its quality. Kinetic model can be used to describe the rate of change in quality attributes as a function of time at a certain temperature. This study was aimed to gain kinetic model of quality deterioration of the red fruit oil during storage. Red fruit oil was packaged in dark bottles at a temperature of 60 °C, 75 °C and 90 °C to test their quality stabilities. The water content, free fatty acid (FFA), peroxide value and total carotenoids were observed for ±15 days. The results showed that the quality of the red fruit oil decreases along with the increase of temperature and storage time. The increased levels of free fatty acids and peroxide value of red fruit oil follows the zero order, with Ea value of 43318 J/mol °K and 29437 J/mol °K, respectively. Red fruit oil peroxide value with the lowest Ea showed the most sensitive to oxidative damage during storage. Shelf life estimation of red fruit oil based on the increasing of FFA and peroxide value are following equation of A=Ao-(t.exp[5,6-4.536(1 /T)]) and A=Ao-(t.exp[4,7-3.541(1/T)), respectively. Whereas decrease of carotenoids content follows first order with Ea value was 78,113 J/mol °K. The shelf life estimation for carotenoids content using equation ln A=ln Ao-(t[20,8-9.395(1/T)) ; where A: content of the end; Ao: initial levels; t: storage time (hours); and T: storage temperature (°K). ABSTRAKMinyak buah merah (Pandanus conoideus) mengandung asam lemak tidak jenuh tinggi sehingga mudah teroksidasi dan mempengaruhi mutunya selama penyimpanan. Penelitian ini bertujuan untuk menentukan stabilitas oksidasi minyak buah merah dan mendapatkan model kinetika kerusakan oksidatif mutu minyak buah merah selama penyimpanan pada suhu kritis. Minyak buah merah yang akan diuji stabilitas mutunya dikemas dalam botol gelap pada suhu 60 °C, 75 °C, dan 90 °C, kemudian dilakukan pengamatan terhadap kadar air, kadar asam lemak bebas (ALB), bilangan peroksida dan total karotenoid selama ±15 hari. Hasil penelitian menunjukkan bahwa mutu minyak buah merah menurun dengan meningkatnya suhu dan lama penyimpanan. Peningkatan kadar ALB dan bilangan peroksida minyak buah merah mengikuti orde nol, dengan nilai Ea berturut-turut 37.709 J/mol °K dan 29.437 J/mol °K; sedangkan penurunan kadar karotenoid mengikuti orde 1 dengan nilai Ea 78.113 J/mol °K. Bilangan peroksida minyak buah merah paling sensitif terhadap kerusakan oksidatif selama penyimpanan karena memiliki nilai Ea terendah. Pendugaan umur simpan minyak buah merah berdasarkan kenaikan nilai ALB dan peroksida mengikuti persamaan A=Ao-(t.eksp[5,6-4.536(1 /T)]) dan A=Ao-(t.eksp[4,7-3.541(1/T)). Sementara umur simpan berdasarkan penurunan kadar karotenoid mengikuti persamaan ln A=ln Ao-(t[20,8-9.395(1/T)) dimana A= kadar akhir; Ao= kadar awal; t= lama penyimpanan (jam); dan T= suhu penyimpanan (°K).
Transesterifikasi In Situ Biji Kemiri (Aleurites moluccana L) Menggunakan Metanol Daur Ulang dengan Bantuan Gelombang Ultrasonik
Get PDFThe purpose of this research was to study the effect of the use of recovered methanol for in situ transesterification reaction candlenut seed (Aleurites moluccana L) as a biodiesel bysonication. The influence of the ratio of recovered methanol to seed, catalyst concentration, reaction time and the temperature was investigated. Important properties of biodiesel such as density, viscosity and acid number were checked according to SNI 7182:2012 standards. The result showed that the maximum yield which has been obtained was 57,85% at recovered methanol to seed ratio 40:1, catalyst concentration 4%, reaction temperature 65 °C and reaction time 80 minutes. The test result of biodiesel properties showed that two parameters (density and viscosity) were conformed to SNI 7182:2012 standards, meanwhile acid value was higher than the maximum standards. In conclusion, the recovered methanol was feasible to produce biodiesel via in situ transesterification but the yield was lower if compared with the use of fresh methanol. ABSTRAKTujuan dari penelitian ini adalah untuk mempelajari penggunaan metanol daur ulang pada reaksi transesterifikasi in situ biji kemiri (Aleurites moluccana L) menjadi biodiesel menggunakan radiasi gelombang ultrasonik. Pengaruh dari rasio metanol daur ulang terhadap biji, jumlah katalis, waktu reaksi dan temperatur telah diteliti. Sifat penting dari biodiesel seperti densitas, viskositas dan bilangan asam telah diuji menurut satandar SNI 7182:2012. Hasil penelitian menunjukkan bahwa rendemen maksimum yang dapat diperoleh adalah 57,85% pada rasio metanol daur ulang tehadap biji 40:1, jumlah katalis 4%, temperatur reaksi 65 °C dan waktu reaksi 80 menit. Hasil pengujian sifat biodiesel menunjukkan bahwa dua paramater (densitas dan viskositas) telah memenuhi standar SNI 7182:2012, sementara bilangan asam lebih tinggi dari standar maksimum. Dapat disimpulkan bahwa metanol daur ulang cocok untuk memproduksi biodiesel secara in situ transesterifikasi tetapi rendemennya lebih rendah jika dibandingkan dengan penggunaan metanol baru.Kata kunci: Biodiesel; kemiri; transesterifikasi in situ; metanol daur ulan
Formulasi Bubuk Minuman Berbahan Dasar Beras Hitam (Oryza sativa L. indica) untuk Lansia Penyandang Diabetes Mellitus Tipe 2
Get PDFThe aim of this research was to get a formula of black rice powder beverage for elderly with type 2 diabetes mellitus. The formulation was done by mixing gelatinized black rice powder with black soybean protein extract, black rice anthocyanin extract, aspartame, and salt based on total energy calculation, taste, and anthocyanin content. The analysis included chemical composition (moisture, ash, total protein, total lipid, and carbohydrate content) of gelatinized black rice, black soybean protein extract, and total anthocyanin content. Nutritional composition, sensory, antioxidant, and recommended dietary allowance of beverage powder was examined. The result showed that each serving with weight 19.91 g/200 mL contained 100 mg of anthocyanin, 10.34 g of carbohydrate, 5.91 g of protein, and 0.77 g of fat. The beverage had 4.8 (neutral-rather like) of preference level based on sensory analysis in elderly. The antioxidant activity based on DPPH radical scavenging ability was 93.33±0.95 % of and could suffice 3.6–4.52% of total energy/serving based on recommended dietary allowance. ABSTRAKPenelitian ini bertujuan untuk mendapatkan formula minuman bubuk berbahan dasar beras hitam untuk lansia penyandang diabetes mellitus (DM) tipe 2. Formulasi minuman bubuk dilakukan dengan mencampur bubuk nasi beras hitam dengan ekstrak protein kedelai hitam, ekstrak antosianin beras hitam, aspartam, dan garam dapur berdasarkan perhitungan energi total, cita rasa, dan kadar antosianin total. Analisis meliputi komposisi kimia (kadar air, abu, protein total, lemak total, dan karbohidrat) bubuk nasi beras hitam, ekstrak protein kedelai hitam dan kadar antosianin total. Pada minuman bubuk hasil formulasi dihitung komposisi nilai gizi, uji kesukaan, aktivitas antioksidan, dan Angka Kecukupan Gizi (AKG). Hasil penelitian menunjukkan bahwa satu takaran saji dengan berat 19,91 g/200 mL mengandung antosianin 100 mg/takaran saji, karbohidrat 10,34 g, protein 5,91 g, dan lemak 0,77 g. Minuman yang dihasilkan memiliki tingkat kesukaan sebesar 4,8 (netral-agak menyukai) berdasarkan uji sensoris pada lansia, memiliki aktivitas antioksidan 93,33±0,95% berdasarkan kemampuan penangkapan radikal DPPH, dan mampu mencukupi 3,68–4,52% kebutuhan energi total/takaran saji berdasarkan AKG lansia per hari.ABSTRACTThe aim of this research was to get a formula of powder beverage made from black rice for elderly with type 2 diabetes mellitus (T2DM). Formulation was done by mixing gelatinized black rice powder, black soybean protein extract, black rice anthocyanin extract, aspartame, and salt based on total energy, taste, and anthocyanin content in the beverage. Gelatinized black rice powder and black soybean protein extract were analyzed for its chemical composition (water, ash, total protein, total lipid, and carbohydrate content), and total anthocyanin content was only analyzed in gelatinized black rice powderto calculated total energy in formulation. The result showed that each serving (19.91 gram/200 ml, contained 100 mg anthocyanin/serving) of beverage powder contained 10.34 gram of carbohydrate, 5.91 gram of protein, and 0.77 gram of fat. The level of elderly preference from sensory analysis was 4.8 (neutral-rather like).It contained 93.33 ± 0,95% of antioxidant activity was determined by DPPH radical scavenging method. The beverage could suffice 3.6-4.52% of total energy based on Recommended DailyAllowance of elderly per day. Keyword: formulation; beverage powder; black rice; elderly; type 2 diabetes mellitusABSTRAKPenelitian ini bertujuan untuk mendapatkan formula bubuk minuman berbahan dasar beras hitam yang ditujukan untuk lansia penyandang diabetes mellitus (DM) tipe 2. Formulasi bubuk minuman dilakukan dengan mencampur bubuk nasi beras hitam dengan ekstrak protein kedelai hitam, ekstrak antosianin beras hitam, aspartam, dan garam dapur berdasarkan perhitungan energi total, cita rasa, dan kadar antosianin total yang dihasilkan minuman. Dilakukan analisis komposisi kimia (kadar air, abu, protein total, lemak total, dan karbohidrat) pada bubuk nasi beras hitam dan ekstrak protein kedelai hitam, serta kadar antosianin total pada bubuk nasi beras hitam sebagai dasar perhitungan dalam formulasi.Perhitungan komposisi nilai gizi, uji kesukaan, aktivitas antioksidan, dan perhitungan Angka Kecukupan Gizi dilakukan pada bubuk minuman hasil formulasi. Hasil penelitian menunjukkan bahwa satu takaran saji (19,91 gram/200 ml, kandungan antosianin sebesar 100 mg/takaran saji) bubuk minuman terpilih mengandung karbohidrat sebesar 10,34 gram, protein 5,91 gram, dan lemak 0,77 gram. Minuman tersebut memiliki tingkat kesukaan lansia dari hasil pengujian sesnsoris sebesar 4,8 (netral-agak menyukai). Aktivitas antioksidan minuman dinyatakan dengan penangkapan radikal DPPH sebesar 93,33 ± 0,95%. Minuman mampu mencukupi 3,68-4,52%kebutuhan energi total/takaran saji berdasarkan Angka Kecukupan Gizi lansia per hari.
Karakteristik Fisikokimia Yogurt Tanpa Lemak dengan Penambahan Whey Protein Concentrate dan Gum Xanthan
Get PDFNon-fat yogurt exhibited weak body, poor texture, and whey separation because of reduction of fat. The separation of whey in yogurt is not desired by consumer. The aim of present study was to evaluate the effect of adding whey protein concentrate (WPC) and xanthan gum on physical, chemical and sensory properties of non-fat yogurt. Physical and chemical properties were tested using randomized block design whereas the yogurts properties during 21 days of cold storage included syneresis index and organoleptic. There were 6 treatments : A (full fat yogurt = control 1); B (skim milk + skim milk powder (SMP) 3% = control 2); C (skim milk + SMP 3% + WPC 1%); D (skim milk + SMP 3% + WPC 1,25%); E (skim milk + SMP 3% + WPC 0,5% + xanthan 0,005%); F (skim milk + SMP 3% + WPC 0,5% + xanthan 0,004%). The fat content 0,12%-0,14% of non-fat yogurt with the addition of WPC and blend of WPC-xanthan could increased firmness, cohesiveness and consistency compared to control 1 and control 2. In the organoleptic properties, non-fat yogurt with the addition of WPC and blend of WPC-xanthan were gained color, flavor, aroma, and consistency of the panelists preferred higher than control yogurt. Non-fat yogurt with only addition of WPC gained the lowest syneresis index. ABSTRAKYogurt tanpa lemak memiliki kekuatan struktur yang rendah dan rentan terjadi pemisahan whey karena berkurangnya kandungan lemak. Pemisahan whey pada yogurt tidak disukai oleh konsumen. Tujuan penelitian ini adalah untuk mengevaluasi efek penambahan whey protein concentrate (WPC) dan gum xanthan terhadap karakteristik fisik, kimia sensori yogurt tanpa lemak. Karakteristik fisik dan kimia diuji menggunakan metode RAK, sedangkan karakteristik yogurt selama penyimpanan dingin 21 hari meliputi indeks sineresis dan organoleptik. Terdapat 6 perlakuan yaitu: A (susu segar = kontrol 1); B (susu skim + skim milk powder (SMP) 3% = kontrol 2); C (susu skim + SMP 3% + WPC 1%); D (susu skim + SMP 3% + WPC 1,25%); E (susu skim + SMP 3% + WPC 0,5% + gum xanthan 0,005%); F (susu skim + SMP 3% + WPC 0,5% + gum xanthan 0,004%). Kandungan lemak 0,12%-0,14% pada yogurt tanpa lemak dengan penambahan WPC dan kombinasi WPC-gum xanthan dapat meningkatkan firmness, cohesiveness, dan konsistensi dibandingkan kontrol 1 dan kontrol 2. Secara organoleptik, yogurt tanpa lemak dengan penambahan WPC dan kombinasi WPC-gum xanthan mendapatkan tingkat kesukaan panelis lebih tinggi untuk warna, rasa, aroma, dan kekentalan dibandingkan yogurt lemak utuh tanpa perlakuan. Yogurt tanpa lemak dengan penambahan WPC saja mendapatkan indeks sineresis lebih rendah dibandingkan perlakuan lainnya
Kinetika Reaksi Penurunan Kafein dan Asam Klorogenat Biji Kopi Robusta melalui Pengukusan Sistem Tertutup
Get PDFThis study was aimed to examine the correlation between temperature and duration of steaming on caffeine and chlorogenic acid reduction as well as to determine energy activation (Ea) according to Arrhenius equation. About 750 g of Robusta coffee was steamed by autoclaving at 100, 110, 120 °C for 1, 2, 3, 4, 5, 6 and 7 hours. The study was conducted in 3 replications. Analyses of caffeine and chlorogenic acid were performed using HPLC. Steaming coffee beans at 100, 110 and 120 °C resulted in the highest decrease of caffeine and chlorogenic acids content for 7 hours by 13%, 18% and 25% for caffeine; and by 37%, 50% and 59% for chlorogenic acids. At all temperatures investigated, decaffeination and chlorogenic acid reduction followed first order reaction. The decaffeination equation at 100 °C, 110 °C and 120 °C followed equation of y = -0.019x + 0.862, y = -0.023x + 0.820, and y = -0.033x + 0.759, respectively. Meanwhile, the chlorogenic acid reduction at 100 °C, 110 °C, and 120 °C followed equation of y = -0.071x + 1.421, y = -0.089x + 1.271, and y = -0.120x + 1.201. Activation energies of decaffeination and chlorogenic acid reduction were 33,543.66 kJ/mol K and 31,934.91 kJ/mol K, respectively. ABSTRAKPenelitian ini bertujuan untuk mengkaji hubungan antara suhu dan lama pengukusan terhadap penurunan kandungan kafein dan asam klorogenat serta menentukan energi aktivasi (Ea) dengan pendekatan persamaan Arrhenius. Biji kopi Robusta masing-masing seberat 750 g dikukus dalam autoklaf (sistem tertutup) pada suhu 100, 110, dan 120 °C masing-masing selama 1, 2, 3, 4, 5, 6, dan 7 jam. Penelitian dilakukan dengan 3 kali ulangan. Analisis kafein dan asam klorogenat dilakukan menggunakan HPLC. Pengukusan biji kopi pada suhu 100, 110, dan 120 °C, menghasilkan penurunan kandungan kafein dan asam klorogenat tertinggi pada pengukusan selama 7 jam. Penurunan kandungan kafein selama 7 jam pada suhu pengukusan 100, 110, dan 120 °C berturut-turut 13%, 18%, dan 25%. Kandungan asam klorogenat mengalami penurunan 37%, 50%, dan 59% berturut-turut pada suhu 100, 110, dan 120 °C. Pada semua suhu yang diuji, penurunan kafein dan asam klorogenat mengikuti reaksi orde satu. Penurunan kafein mengikuti persamaan y = -0,019x + 0,862, y = -0,023x + 0,820, dan y = -0,033x + 0,759, sedangkan untuk asam klorogenat mengikuti persamaan y = -0,071x + 1,421, y = -0,089x + 1,271, dan y = -0,120x + 1,201. Besarnya energi aktivasi penurunan kafein dan asam klorogenat berturut turut adalah 33.543,66 kJ/molK dan 31.934,91 kJ/Mok
Initial Moisture Content of Corncobs Plays an Important Role in Maintaining its Quality during Storage
Get PDFMaize is one of the important staple foods for people in Timor, East Nusa Tenggara Province, Indonesia. Subsistent farmers store the maize for their own consumption until the next harvest season, for seed and feed. However, high initial water content of the kernel due to improper drying prior storage initiate serious damage and losses during the maize storage. High water content promotes the growth of fungi and insects, and increase respiration rate, resulting in rapid deterioration of maize. The purpose of this study was to determine the initial moisture content that might minimize damage and losses of maize in the farmers’ storage, and to study the effects of some plant materials that are used to smoke corns before storage. The experiment was initiated by sun-drying the harvested corncobs for 0, 2, 4, 6, 8, and 10 days (6 hours a day). This experiment was designed using Completely Randomized Design with 6 treatments and 3 replications. Dried corncobs were stored in the farmer’s storage for 4 months. The effects of maize kernels’ initial water content on the development of water content in kernels; the percentage of damaged kernels; and the species of pathogen and insects were investigated during storage with 2-week intervals. The results demonstrated that drying the corncobs prior storage for 10 days, resulting in 12.96% of water content, significantly decreased the percentage of seed damage to 6.5%, as compared to without drying process which resulted in 63%. Aspergillus flavus, Fusarium sp., and Penicillium sp were found to be the main pathogen during storage. There are no insect pests found during the storage.
Karakteristik Fisikokimia Gelatin Tulang Ikan Patin (Pangasius sutchi) Hasil Ekstraksi Menggunakan Limbah Buah Nanas (Ananas comosus)
No full textFish bones are the most potential as an alternative source for gelatin production. Pangasius catfish bone is the one of the most promising source due to its high yield of gelatin. It has similar gel strength with the commercial gelatin and has a low ash content. The aim of this research was to determine the physical and chemical characteristics of Pangasius catfish bone gelatin extracted using pineapple waste. Pineapple waste has a low pH and contains citric acid. The research was conducted in two stages i.e. gelatin extraction and physicochemical characterization. Gelatin extraction was done by pre-treatment and main extraction. Pre-treatment was done by soaking the fish bone into pineapple waste for 32, 48, and 56 hours. The main extraction was carried out by soaking fish ossein in warm water for 5 hours at 75 °C. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the gelatin existence. Fish bone gelatin has molecular weight ranging from 100-150 kDa and > 225 kDa. The hydroxyproline content of gelatin was 10.9–16.3 mg/g. Gelatin of Pangasius catfish bone extracted with pineapple waste has gel strength of 64.83 g.bloom g, hardness of 4.96 N, cohesiveness of 0.88, springiness of 1.03 mm, gumminess of 4.36 N, and chewiness of 2.78 N. Viscosity and pH of gelatin solution obtained were 3.17 cP and 4.52 respectively. The proximate characteristics obtained were moisture 8.59%, ash 0.95%, crude protein 47.60% and fat 7.71%. Some physicochemical parameters of gelatin resemble commercial gelatin and other fish bone gelatin. ABSTRAKTulang ikan merupakan salah satu sumber alternatif untuk produksi gelatin yang sangat potensial. Gelatin dari tulang ikan patin yang diekstrak dengan asam diketahui memiliki hasil ekstraksi yang tertinggi, kekuatan gel yang menyerupai gelatin komersial dan kadar abu yang rendah. Penelitian ini dilakukan untuk mengetahui karakteristik fisik dan kimiawi gelatin tulang ikan patin yang diekstrak dengan limbah buah nanas. Limbah buah nanas diketahui mengandung asam sitrat cukup tinggi. Penelitian dilakukan dengan dua tahap yakni ekstraksi gelatin dan analisis karakteristik fisikokimianya. Ekstraksi gelatin dilakukan dengan dua tahap yakni pre-treatment dan ekstraksi utama. Pre-treatment dilakukan dengan perendaman tulang ikan dalam limbah buah nanas selama 32, 48, dan 56 jam. Ekstraksi utama dilakukan dengan perendaman dalam air hangat selama 5 jam suhu 75 °C. Analisis keberadaan gelatin dengan metode SDS-PAGE diketahui gelatin berada pada kisaran berat molekul 100–150 kDa dan > 225 kDa. Kadar hidroksiprolin gelatin yakni 10,9–16,3 mg/g. Gelatin tulang ikan patin yang diekstrak dengan limbah buah nanas memiliki kekuatan gel 64,83 g.bloom, kekerasan (hardness) 4,96 g, kohesivitas (cohesiveness) 0,88, elastisitas (springiness) 1,03, kekenyalan (gumminess) 4,36, daya kunyah (chewiness) 2,78. Viskositas dan pH larutan gelatin yang diperoleh masing-masing 3,17 cP dan 4,52. Karakteristik kimiawi gelatin yang diperoleh antara lain meliputi kadar air 8,59%, abu 0,95%, protein kasar 47,60% dan lemak 7,71%. Beberapa karakteristik fisikokimia gelatin tulang ikan pada penelitian ini dapat dibandingkan dengan gelatin komersial dan gelatin tulang ikan yang lain
Temperature and Air Velocity Simulation on Sago Starch Pneumatic Conveying Recirculated Dryer Using Ansys Fluent
Get PDFPneumatic Conveying Recirculated Dryer (PCRD) is one of many driers used for drying wet sago starch. The most important components of this PCRD machine are the vertical pipe and the u-bend. The vertical pipe and the u-bend are the primary drying spaces. They must have a good temperature distribution and air velocity dryer. To observe the process of temperature distribution and the air velocity dryer in the vertical pipe and u-bend, Computational Fluid Dynamics (CFD) analysis is required. The research was aimed to analyze the temperature distribution and the air velocity dryer flow in the recirculated pipe of PCRD machine by using CFD analysis. The analysis was based on the variance of the temperature, the air velocity, and the height of the vertical pipe in PCRD machine. The analysis was conducted using Ansys Workbench Fluid Flow ver. 15. This software was used to simulate the temperature and the air flow velocity in the vertical pipe and the u-bend. However, the flow characteristics and patterns of the wet sago starch were not included in the discussion. The turbulence model used in the simulation was the Reynold Stress Models (RSM). The result of the simulation showed that the temperature along the vertical pipe and the u-bend was distributed evenly. The error value between the result of the simulation and the observation was low (0.10–2.04%). The average test value with paired t-test showed that the simulation and observation result was not significantly different. This results indicated that the simulation fit well with the observation value or the real condition in the PCRD machine. The distribution of the temperature and the air velocity dryer in the vertical pipe and the u-bend were able to reduce the moisture content on sago starch from 31% (wb) to 9% (wb). Therefore, the vertical pipe and the u-bend design was appropriate to use in PCRD machine for drying wet sago starch