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Aktivitas Antioksidan Ekstrak Etanol Daun Pandan (Pandanus amaryllifolius) dan Fraksi-Fraksinya
No full textFractionation process of pandan leaf (Pandanus amaryllifolius) extract could enhance its antioxidant activity as higher purity of bioactive compounds was achieved. The aims of this study were to determine the antioxidant activity of ethanol extract fractions of pandan leaves and to determine the fraction that has the highest antioxidant activity. The research was divided into three stages: (1) extraction and fractionation of pandan leaf extract, (2) phytochemical analysis of pandan leaf fractions, and (3) analysis of antioxidative activity. The analysis of antioxidant activity includes reducing power approach by ferrithiocyanate (FTC), DPPH radical scavenging, and the calculation of EC50. The results showed that the ethyl acetate fraction of ethanol extract of pandan leaves had a higher reductive ability than the ethanol extract, while its DPPH radical scavenging activity was lower. Phenolic and flavonoid compounds were quantified in ethyl acetate fraction > ethanol extract > hexane fraction. Ethanol extract of pandan leaves and ethyl acetate fraction qualitatively contained alkaloid, flavonoid, phenolic, and saponin. Terpenoid compounds were present in ethanol extract whereas steroid compounds were present in ethyl acetate fraction. Hexane fraction only contained steroid and phenolic compounds. Higher reducing power values were ethyl acetate fraction, ethanol extract, hexane fraction, and vitamin E commercial respectively. Ethyl acetate fraction had higher antioxidant activity value (FTC method) than hexane fraction and ethanol extract. Ethyl acetate had a higher DPPH radical scavenging activity value than vitamin E. Ethyl acetate fraction, hexane fraction, ethanol extract, and vitamin E commercial had EC50 values of 0.90; 8.66; 4.51; and 11.76 mg/mL respectively. Ethyl acetate fraction of ethanol extract of pandan leaves is a potential source of natural antioxidant.ABSTRAKProses fraksinasi ekstrak daun pandan (Pandanus amaryllifolius) dapat meningkatkan aktivitas antioksidannya karena dengan proses fraksinasi akan diperoleh komponen bioaktif yang lebih murni. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari fraksi-fraksi ekstrak etanol daun pandan dan menentukan fraksi yang mempunyai aktivitas antioksidan yang tinggi. Penelitian dibagi dalam 3 tahap yaitu ekstraksi dan fraksinasi ekstrak daun pandan, analisis fitokimia fraksi-fraksi daun pandan, dan uji aktivitas antioksidannya. Uji aktivitas antioksidan meliputi analisis daya mereduksi dengan metode feritiosianat (FTC) dan daya tangkap radikal DPPH serta perhitungan EC50. Hasil penelitian menunjukkan bahwa fraksi etil asetat dari ekstrak etanol daun pandan mempunyai kemampuan mereduksi lebih tinggi dibanding ekstrak etanolnya, namun daya tangkap radikal DPPH-nya lebih rendah. Komponen fenol dan flavonoid yang terkandung dalam fraksi etil asetat > esktrak etanol > heksan. Ekstrak etanol daun pandan dan fraksi etil asetat secara kualitatif mengandung alkaloid, flavonoid, fenol, dan saponin. Senyawa terpenoid terdapat pada ekstrak etanol sedangkan steroid terdapat pada fraksi etil asetat. Fraksi heksan hanya mengandung senyawa steroid dan fenolik. Daya mereduksi yang dimiliki fraksi etil asetat > ekstrak etanol > heksan > vitamin E komersial. Aktivitas antioksidan yang dimiliki BHT > vitamin E > fraksi etil asetat > ekstrak etanol > fraksi heksan. Daya tangkap radikal DPPH dari BHT > ekstrak etanol > fraksi heksan atau fraksi etil asetat>. Fraksi etil asetat, fraksi heksan, ekstrak etanol, dan vitamin E komersial mempunyai nilai EC50 berturut-turut sebesar: 0,90; 8,66; 4,51; dan 11,76 mg/mL. Fraksi etil asetat ekstrak etanol daun pandan berpotensi sebagai sumber antioksidan alami.Kata kunci: Ekstrak etanol daun pandan; fraksinasi; antioksidan; daya mereduksi; DPPHFractionation process of pandan leaf (Pandanus amaryllifolius) extract could enhance its antioxidant activity as higher purity of bioactive compounds was achieved. The aims of this study were to determine the antioxidant activity of ethanol extract fractions of pandan leaves and to determine the fraction that has the highest antioxidant activity. The research was divided into three stages: (1) extraction and fractionation of pandan leaf extract, (2) phytochemical analysis of pandan leaf fractions, and (3) analysis of antioxidative activity. The analysis of antioxidant activity includes reducing power approach by ferrithiocyanate (FTC), DPPH radical scavenging, and the calculation of EC50. The results showed that the ethyl acetate fraction of ethanol extract of pandan leaves had a higher reductive ability than the ethanol extract, while its DPPH radical scavenging activity was lower. Phenolic and flavonoid compounds were quantified in ethyl acetate fraction > ethanol extract > hexane fraction. Ethanol extract of pandan leaves and ethyl acetate fraction qualitatively contained alkaloid, flavonoid, phenolic, and saponin. Terpenoid compounds were present in ethanol extract whereas steroid compounds were present in ethyl acetate fraction. Hexane fraction only contained steroid and phenolic compounds. Higher reducing power values were ethyl acetate fraction, ethanol extract, hexane fraction, and vitamin E commercial respectively. Ethyl acetate fraction had higher antioxidant activity value (FTC method) than hexane fraction and ethanol extract. Ethyl acetate had a higher DPPH radical scavenging activity value than vitamin E. Ethyl acetate fraction, hexane fraction, ethanol extract, and vitamin E commercial had EC50 values of 0.90; 8.66; 4.51; and 11.76 mg/mL respectively. Ethyl acetate fraction of ethanol extract of pandan leaves is a potential source of natural antioxidant.ABSTRAKProses fraksinasi ekstrak daun pandan (Pandanus amaryllifolius) dapat meningkatkan aktivitas antioksidannya karena dengan proses fraksinasi akan diperoleh komponen bioaktif yang lebih murni. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari fraksi-fraksi ekstrak etanol daun pandan dan menentukan fraksi yang mempunyai aktivitas antioksidan yang tinggi. Penelitian dibagi dalam 3 tahap yaitu ekstraksi dan fraksinasi ekstrak daun pandan, analisis fitokimia fraksi-fraksi daun pandan, dan uji aktivitas antioksidannya. Uji aktivitas antioksidan meliputi analisis daya mereduksi dengan metode feritiosianat (FTC) dan daya tangkap radikal DPPH serta perhitungan EC50. Hasil penelitian menunjukkan bahwa fraksi etil asetat dari ekstrak etanol daun pandan mempunyai kemampuan mereduksi lebih tinggi dibanding ekstrak etanolnya, namun daya tangkap radikal DPPH-nya lebih rendah. Komponen fenol dan flavonoid yang terkandung dalam fraksi etil asetat > esktrak etanol > heksan. Ekstrak etanol daun pandan dan fraksi etil asetat secara kualitatif mengandung alkaloid, flavonoid, fenol, dan saponin. Senyawa terpenoid terdapat pada ekstrak etanol sedangkan steroid terdapat pada fraksi etil asetat. Fraksi heksan hanya mengandung senyawa steroid dan fenolik. Daya mereduksi yang dimiliki fraksi etil asetat > ekstrak etanol > heksan > vitamin E komersial. Aktivitas antioksidan yang dimiliki BHT > vitamin E > fraksi etil asetat > ekstrak etanol > fraksi heksan. Daya tangkap radikal DPPH dari BHT > ekstrak etanol > fraksi heksan atau fraksi etil asetat>. Fraksi etil asetat, fraksi heksan, ekstrak etanol, dan vitamin E komersial mempunyai nilai EC50 berturut-turut sebesar: 0,90; 8,66; 4,51; dan 11,76 mg/mL. Fraksi etil asetat ekstrak etanol daun pandan berpotensi sebagai sumber antioksidan alami.Kata kunci: Ekstrak etanol daun pandan; fraksinasi; antioksidan; daya mereduksi; DPP
Study of Beef Availability Potential in Yogyakarta Special Province (DIY) through Multi Criteria Analysis (MCA) Model by Spatial Geographic Information System
Get PDFThe increasing human population and income per capita in Indonesia have many impacts to the demand of food, not only staple food but also the secondary food too, including meat and beef demand. Based on data from Central Statistics Bureau of 1995–2016, beef demand in Indonesia has been increasing. This condition must be anticipated by preparing the supply, especially the availability of beef in Daerah Istimewa Yogyakarta province. In Yogyakarta city, one of the regencies of DIY, the demand rate grows by 3.2%/year, higher than the supply growth rate by 2.08 %/year. This gap needs to be thought through the case of scarcity of beef on the market. This study aimed to identify the potential of supply in five parameters i.e.: beef population, the availability of semen for artificial insemination (IB), the availability of livestock feed plants (HMT), the number of cows that enter DIY, and the number of cows that exit from DIY. A model Spatial Geographic Information Systems (SGIS) has been used in this study. The study revealed the most potential area for the development of beef agribusiness, based on the availability side, was Gunung Kidul Regency, and the less potential was Yogyakarta municipality
Efek Antioksidatif dan Akseptabilitas Bakso Daging Ayam Ras dengan Penambahan Gel Lidah Buaya
Get PDFCurrently, the consumption of broiler-chicken meat increases up to 3.7510 kg/cap/year. This condition is due to the increasing of population growth and food businesses which use chicken meat. On the other hand, the chicken meat contains of high fat, thus easily oxidized and resulted in free radicals and off- flavor. Chicken meat is widely used as meatball that is processed through the stage of meat-milling and boiling which triggers fat oxidation. Therefore, an antioxidant is needed to inhibit oxidation. Aloevera contains flavonoid compounds that could scavange free radicals. The objective of this research was to optimize the addition of aloe vera gel in the chicken meatball to obtain high antioxidant effect and acceptable product. Considering its high water content (> 98%), in this study aloe vera gel was added as an ice-substitution in the making of meatball. The meatballs were made by mixing of 250 g chicken meat with 125 g tapioca for each sample, seasoning (salt, pepper, garlic), and added ice with water/aloe vera gel ratio (g/g) of 100/0; 85/15; 70/30; 55/45 and 40/60. The doughs were molded in ball-shape then boiled at 90 oC for 10 minutes. The meatballs were subjected to analysis of moisture content, the ability to scavenge of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical or radical scavenging activity (RSA), acid, peroxide and thyobarbituric acid (TBA) number, physical properties including water holding capacity (WHC), cooking loss, and texture (softness, chewiness and deformation) as well as acceptability by hedonic test. The results showed that the highest acceptability and antioxidative activity of meatballs was obtained in meatball with the ratio of water/gel between 55/45- 40/60 (g/g). This aforementioned meatball had softer texture and likely chewiness. The percentage of the WHC and cooking loss were not significantly different, but it had high RSA and lowest value of acid, peroxide and TBA numbers. ABSTRAKSaat ini konsumsi daging ayam ras pedaging meningkat hingga mencapai 3,7510 kg/kap/tahun. Hal ini disebabkan antara lain oleh meningkatnya jumlah penduduk dan usaha di sektor pangan yang menggunakan daging ayam ras. Disisi lain, daging ayam ras mengandung lemak cukup tinggi, sehingga mudah teroksidasi menghasilkan radikal bebas dan penurunan flavor. Daging ayam ras banyak digunakan sebagai bakso yang diolah melalui proses penggilingan daging dan perebusan, sehingga dapat memicu terjadinya oksidasi lemak. Oleh sebab itu, diperlukan antioksidan untuk menghambat oksidasi. Aloe vera diketahui mengandung senyawa flavonoid yang dapat menangkal radikal bebas. Tujuan penelitian ini adalah melakukan optimasi penambahan gel lidah buaya pada pembuatan bakso daging ayam ras untuk menghasilkan bakso dengan efek antioksidatif tinggi dan disukai. Pada penelitian ini gel lidah buaya ditambahkan untuk mensubtitusi es batu yang digunakan pada pembuatan bakso, mengingat kandungan air gel tinggi (> 98%). Pembuatan bakso untuk setiap 250 g daging ayam ditambah 125 g tapioka, bumbu (garam, merica, bubuk bawang putih) dan es batu yang dibuat dengan variasi rasio air/gel lidah buaya (g/g): 100/0; 85/15; 70/30; 55/45 dan 40/60. Bahan dicampur menjadi adonan dan dibuat bola-bakso, selanjutnya direbus pada suhu 90 oC selama 10 menit. Analisis yang dilakukan adalah kadar air, kemampuan menangkap radikal DPPH, angka asam, peroksida dan TBA (thyobarbituric acid); sifat fisik: water holding capacity (WHC), cooking loss, tekstur (kelunakan, kekenyalan dan deformasi) dan akseptabilitas bakso ditentukan secara inderawi dengan Hedonic Test. Hasil penelitian menunjukkan bahwa bakso dengan akseptabilitas dan aktivitas antioksidasi tinggi dibuat dengan es batu dengan rasio air/gel lidah buaya 55/45-40/60 (g/g). Bakso tersebut lebih lunak dan kekenyalannya disukai. Persentase WHC dan cooking loss tidak berbeda nyata, nilai RSA tinggi, sedangkan angka asam, peroksida dan TBA paling rendah
Fermentasi Biji Kakao Kering Menggunakan Saccharomyces cerevisiae, Lactobacillus lactis, dan Acetobacter aceti
No full textThe aims of the study was to improve quality of cocoa bans by fermentation of sun dried cocoa beans. The fermentation variations were conducted as follows: first, fermentation without the addition of inoculum (control), the second treatment using inoculum of S. cerevisiae (FNCC 3056), L. lactis (FNC 0086) and A. aceti (FNCC 0016), each of 108 cfu/g given simultaneously at the beginning of fermentation.and the third treatment wassequential administration, i.e: yeast at the initial fermentation, lactic acid bacteria after 24 hours fermentation, and acetic acid bacteria after 48 hr of fermentation third with the same microbial population with the second treatment. The fermentation was conducted for120 hours. The fermentation temperature were controlled during fermentation as follows: 35 °C for the first 24 hours, 45 °C for the next second 24- hours, 55 °C the third 24 hours and 35 °C for the last 48 hours of fermentation. The results showed that after the rehydration, pulp composition of dry beans could be used as a substrate for fermentation. During fermentation, dry cocoa beans showed reduction of total sugar content, pH and total polyphenols for all the three treatments. Cut test of dried cocoa beans during the fermentation showed the increasing percentage of brown color of the three treatments. Reducing sugar and fermentation indexes increasedfor all treatments during fermentation. Concentration of ethanol, lactic acid and acetic acid reached highest level at 24, 60, and 108 hours of fermentationfor all treatments. Highest populations of S. cerevisiae, L. lactis and A. aceti of three treatments obtained at 24, 48 and 72 hours of fermentation. After fermentation and roasting, dry beans produced hydrophobic amino acids as precursors of flavor and volatile compounds. ABSTRAKPenelitian ini bertujuan untuk mengetahui perubahan sifat kimia pada fermentasi biji kakao kering jemur. Biji kakao kering jemur yang diperoleh dari petani memiliki kadar air yang tidak seragam. Guna menimalkan kegagalan fermentasi maka biji kakao kering jemur diperoleh melalui pengeringan biji kakao segar menggunakan kabinet dryer dengan sebelumnya dikondisikan pada suhu seperti pengeringan dengan sinar matahari, dan masing ditentukan kadar gula reduksinya. Percobaan fermentasi biji kakao kering dilakukan fermentasi pada wadah fermentasi dengan jumlah biji 150 g setiap wadah. Sebelum difermentasi terlebih dahulu biji kakao kering jemur direhidrasi agar didapat kadar air mendekati biji segar, kemudian biji kakao kering jemur diinkubasi selama enam hari dan tanpa dibalik selama fermentasi. Setiap perlakuan diulangi tiga kali dan diamati tiap 24 jam sampai 120 jam. Kadar gula reduksi (kontrol 4,49–11,45%, inokulum diawal (IA) 4,69–11,55%, inokulum bertahap (IB) 4,64–11,54%), kadar asam tertitrasi (kontrol 4,48–6,45%, inokulum diawal (IA) 4,64–6,39%, inokulum bertahap (IB) 4,45–6,59%), populasi Saccharomycescerevisiae (kontrol 5,56–7,28 (log CFU/g), inokulum diawal (IA) 6,45–8,75 (logCFU/g), inokulum bertahap (IB) 6.88 – 8.99 (logCFU/g), Lactobacillus lactis (kontrol 6,66–8,15 (log CFU/g), inokulum diawal (IA) 7,65–8,21(log CFU/g), inokulum bertahap (IB) 7,66–8,95 (log CFU/g) dan Acetobacter aceti (kontrol 4,26–6,95% (log CFU/g), inokulum diawal (IA) 4,85–7,40 (log CFU/g), inokulum bertahap (IB) 4,35–7,91 (log CFU/g)) dalam pulp fermentasi diamati selama proses fermentasi. Untuk mengetahui kualitas biji kakao dilakukan pengukuran pH (kontrol 5,67–3,98, inokulum diawal (IA) 5,67–3,55, inokulum bertahap (IB) 5,67–3,50), kadar etanol (kontrol 0,3–0,5%, inokulum diawal (IA) 0,3–0,52%, inokulum bertahap (IB) 0,35–0,53%) dan indeks fermentasi selama fermentasi (kontrol 0,31–0,88, inokulum diawal (IA) 0,32–0,99, inokulum bertahap (IB) 0,33–1,03).Kata kunci: Acetobacter aceti; biji kakao kering jemur; fermentasi; Lactobacillus lactis; Saccharomyces cerevisia
Validasi Metode Analisis Kadar Kalsium pada Susu Segar secara Titrasi Kompleksometri
Get PDFAnalysis of calcium content in fresh milk generally uses Atomic Absorption Spectroscopy (AAS). Alternatively, it could be analyzed using complexometric titration. The study aimed to validate the method of calcium content analysis in fresh milk by complexometric titration. The results showed that the linearity test using calcium standard solution at a working range of 4-24 mg/100 mL had R2 of 0.9983, while the limit of detection and limit of quantification were 0.47 mg/100 mL and 1.57 mg/100 mL, respectively. The accuracy by spiking method at the spiking concentration of 60 mg/100 mL sample was 99.29%. The repeatability and intra-lab reproducibility showed acceptable precision with CV value of 0.98% and 2.59%, respectively. The results of ruggedness test showed that this method was rugged to the variation of sample volume and ethylenediaminetetraacetic acid (EDTA) concentration. This study proved that complexometric titration can be used to determine calcium content in fresh milk. ABSTRAKAnalisis kadar kalsium pada susu segar umumnya menggunakan Atomic Absorption Spectroscopy (AAS) yang relatif mahal. Salah satu metode alternatif yang dapat digunakan adalah titrasi kompleksometri. Sampai sekarang, data validasi terhadap metode tersebut pada sampel susu segar belum tersedia. Tujuan penelitian ini adalah memvalidasi metode analisis kadar kalsium pada susu segar secara titrasi kompleksometri. Hasil penelitian menunjukkan uji linearitas dengan menggunakan larutan standar kalsium pada rentang 4-24 mg/100 mL mempunyai nilai R2 sebesar 0,9983, sedangkan nilai batas deteksi dan batas kuantifikasi berturut-turut adalah 0,47 mg/100 mL dan 1,57 mg/100 mL. Nilai akurasi (recovery) sebesar 99,29% pada konsentrasi spiking 60 mg/100 mL sampel. Hasil penelitian atau metode uji yang divalidasi menunjukkan nilai keterulangan dan reprodusibilitas intralab yang baik dengan nilai CV analisis berturut-turut 0,98% dan 2,59%. Hasil uji ketangguhan menunjukkan metode ini bersifat tangguh (rugged) terhadap variasi volume sampel dan konsentrasi asam etilenadiaminatetraasetat (EDTA) yang digunakan. Berdasarkan hasil penelitian tersebut, metode titrasi kompleksometri dapat digunakan untuk menentukan kadar kalsium pada susu segar.
Evaluasi Kesesuaian Lahan Komoditas Pangan Utama di Kecamatan Umbu Ratu Nggay Barat Kabupaten Sumba Tengah
Get PDFThis research was conducted in West Umbu Ratu Nggay district of Central Sumba Region in January–March 2017. The method of analysis used was LQ (Location Quotient) method, matching method and overlay method. The results showed that the maim food commodities in West Umbu Ratu Nggay District were green beans with values of LQ 1,60; sweet potato with LQ 1,33, rice with LQ 1,16; and corn with LQ 1,03. Green beans suitability class is S2 (moderatelly suitable) with area 28.021,13 ha (99.69%) and N (not suitable) with area 87.91 ha (0.31%). Sweet potato commodity with land suitability is in class S1 (highly suitable) with area 90,41 ha (0.32%), S2 (moderatelly suitable) with of 27.930,73 ha (99.7%), and N (not suitable) with area 87,91 ha (0.31%). Commodity of rice with land suitability class S1 (highly suitable) with area of 1.322,43 ha (4.70%), S2 (moderatelly suitable) with area of 26.704,21 ha (95.00%), and N (not suitable) with area of 83,24 ha (0.30%). Corn commodity with land suitability class S2 (moderatelly suitable) with area of 8.21,13 ha (99.69%) and N (not suitable) with area of 87,91 ha (0.31%). ABSTRAKPenelitian ini dilakukan di Kecamatan Umbu Ratu Nggay Barat Kabupaten Sumba Tengah pada bulan Januari-Maret 2017. Metode analisis yang digunakan adalah metode LQ (Location Quotient), metode pencocokan dan metode overlay. Hasil analisis menunjukkan bahwa komoditas pangan unggulan di Kecamatan Umbu Ratu Nggay Barat secara berturut-turut adalah kacang hijau dengan nilai LQ 1,60, ubi jalar dengan nilai LQ 1,33, padi sawah dengan nilai LQ 1,03 dan jagung dengan Nilai LQ 1,03. Kelas kesesuaian lahan kacang hijau adalah S2 (cukup sesuai) dengan luas 28.021,13 ha (99,69%) dan N (tidak sesuai) 87,91 ha (0,31%). Komodias ubi jalar dengan kelas kesesuaian lahan S1 (sangat sesuai) dengan luas 90, 41 ha (0,32%), S2 (cukup sesuai) dengan luas 27.930,73 ha (99,37%), dan N (tidak sesuai) 87,91 ha (0,31%). Komoditas padi sawah dengan kelas kesesuaian lahan S1 (sangat sesuai) 1.322, 43 ha (4,70%). S2 (cukup sesuai) 26.704,21 ha (95,00%), dan N (tidak sesuai) 83,24 ha (0,30%). Komoditas jagung dengan kelas kesesuaian lahan S2 (cukup sesuai) 28.021,13 ha (99,69%) dan N (tidak sesuai) 87,91 ha (0,31%).
Pengaruh Pelapis dapat Dimakan dari Karagenan terhadap Mutu Melon Potong dalam Penyimpanan Dingin
Get PDFRecently, fresh-cut melon consumption has been increasing due to the health benefits. One of methods to reduce the decay of quality by minimal processing of fruits is edible packaging. This method may extend the shelf-life of fresh-cut melon. The objective of this research was to study the effectiveness of the edible coatings and edible films to maintain the quality of fresh-cut melon during storage at 10°C for 6 days. The edible solutions were prepared by dissolving carrageenan of 2%, glycerol of 1%, and tween 80 of 0.2% into aquadest. These solutions were used as coating systems by dipping and wrapping method. Uncoated fruits served as controls. Quality was measured in terms of weight loss, hardness, soluble solids, pH value, and total plate count. The result revealed that for all treatments the value of weight loss, soluble solid content, and total plate count increased during storage whilst hardness, pH, decreased. In terms of microbiological, the coating improved the shelf-life of fresh-cut melon (up to 2 days) compared to the controls. ABSTRAKKonsumsi buah melon potong segar meningkat beberapa tahun terakhir karena manfaat kesehatannya. Salah satu alternatif terbaru untuk mengurangi penurunan kualitas yang disebabkan oleh pengolahan minimal buah-buahan adalah pengembangan edible packaging (kemasan dapat dimakan). Mereka diharapkan dapat memperpanjang umur simpan buah melon potong. Tujuan penelitian untuk mempelajari efektivitas edible coating dan edible film untuk menjaga kualitas buah melon potong segar selama penyimpanan pada temperatur 10oC selama 6 hari. Larutan edible dibuat dengan melarutkan 2% karagenan, 1% gliserol, dan 0,5% tween 80 dalam aquadest pada temperatur 70-80 °C. Larutan ini digunakan sebagai sistem pelapisan dengan metode pencelupan dan metode pembungkusan. Buah melon tanpa pelapis disiapkan sebagai kontrol. Pengukuran kualitas buah melon meliputi susut bobot, kekerasan, total padatan terlarut, nilai pH, dan total plate count. Hasil penelitian menunjukkan bahwa untuk semua perlakuan terjadi peningkatan susut bobot, total padatan terlarut, dan angka lempeng total selama penyimpanan, sementara kekerasan dan pH mengalami penurunan. Dalam hal mikrobiologi, pelapisan meningkatkan masa simpan buah melon potong (hingga 2 hari) dibandingkan dengan kontrol.Kata kunci: Karagenan; edible coating; edible film; melonRecently, fresh-cut melon consumption has been increasing due to the health benefits. One of methods to reduce the decay of quality by minimal processing of fruits is edible packaging. This method may extend the shelf-life of fresh-cut melon. The objective of this research was to study the effectiveness of the edible coatings and edible films to maintain the quality of fresh-cut melon during storage at 10°C for 6 days. The edible solutions were prepared by dissolving carrageenan of 2%, glycerol of 1%, and tween 80 of 0.2% into aquadest. These solutions were used as coating systems by dipping and wrapping method. Uncoated fruits served as controls. Quality was measured in terms of weight loss, hardness, soluble solids, pH value, and total plate count. The result revealed that for all treatments the value of weight loss, soluble solid content, and total plate count increased during storage whilst hardness, pH, decreased. In terms of microbiological, the coating improved the shelf-life of fresh-cut melon (up to 2 days) compared to the controls. ABSTRAKKonsumsi buah melon potong segar meningkat beberapa tahun terakhir karena manfaat kesehatannya. Salah satu alternatif terbaru untuk mengurangi penurunan kualitas yang disebabkan oleh pengolahan minimal buah-buahan adalah pengembangan edible packaging (kemasan dapat dimakan). Mereka diharapkan dapat memperpanjang umur simpan buah melon potong. Tujuan penelitian untuk mempelajari efektivitas edible coating dan edible film untuk menjaga kualitas buah melon potong segar selama penyimpanan pada temperatur 10oC selama 6 hari. Larutan edible dibuat dengan melarutkan 2% karagenan, 1% gliserol, dan 0,5% tween 80 dalam aquadest pada temperatur 70-80 °C. Larutan ini digunakan sebagai sistem pelapisan dengan metode pencelupan dan metode pembungkusan. Buah melon tanpa pelapis disiapkan sebagai kontrol. Pengukuran kualitas buah melon meliputi susut bobot, kekerasan, total padatan terlarut, nilai pH, dan total plate count. Hasil penelitian menunjukkan bahwa untuk semua perlakuan terjadi peningkatan susut bobot, total padatan terlarut, dan angka lempeng total selama penyimpanan, sementara kekerasan dan pH mengalami penurunan. Dalam hal mikrobiologi, pelapisan meningkatkan masa simpan buah melon potong (hingga 2 hari) dibandingkan dengan kontrol.Kata kunci: Karagenan; edible coating; edible film; melon
Komposisi Asam Lemak, Angka Peroksida, dan Angka TBA Fillet Ikan Kakap (Lutjanus sp) pada Suhu dan Lama Penyimpanan Berbeda
No full textFish has a high nutritional value and is a major food source in many countries. Fish lipid has a high content of polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA; 20: 5n-3) and docosahexsanoic acid (DHA; 22: 6n-3). The objective of this research was to determine fatty acids composition of snapper (Lutjanus sp) fillet and its damage during the storage process. The results showed that total of the saturated fatty acids (SFA) increased from 4.35% to 25.55%, 28.06%, 32.73%, and 61.75% during storage at 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C, respectively. Total mono-unsaturated fatty acids (MUFA) were 23.72%, 23.69%, 14.4%, 22.66%, and 29.4% at storage temperature of 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C. Total PUFA decreased from 25.06% to 15.98%, 14.99%, 10.32%, and 8.84% at 0 °C, 10 °C, 20 °C, 30 °C, and 40 °C. Peroxide value, as primary peroxide of snapper fillet, increased about 10.60 times with an increased in storage temperature from 0 °C to 40 °C. Value of TBA increased 6.60 times with an increased in temperature from 0 °C to 40 °C during 45 days.ABSTRAKIkan memiliki nilai gizi tinggi dan merupakan sumber makanan utama di banyak negara. Lipid ikan memiliki kandungan tinggi asam tak jenuh ganda (Poly Unsaturated Fatty Acid, PUFA), terutama asam eikosapentanoat (EPA; 20:5n-3) dan asam docosahexsanoat (DHA; 22:6n-3). Penelitian ini bertujuan untuk mengetahui komposisi asam lemak fillet ikan kakap (Lutjanus sp) dan kerusakan akibat proses penyimpanan. Hasil analisis asam lemak jenuh (Saturated Fatty Acid, SFA) menunjukkan bahwa asam lemak jenuh meningkat dari 4,35% menjadi 25,55%, 28,06%, 32,73%, dan 61,75% selama penyimpanan pada 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Total asam lemak tak jenuh (Mono Unsaturated Fatty Acid, MUFA) adalah 23,72%, 23,69, 14,4%, 22,66%, dan 29,4% pada penyimpanan 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Sedangkan total PUFA turun dari 25,05% menjadi 15,98%, 14,99%, 10,32%, dan 8,84% pada penyimpanan 0 °C, 10 °C, 20 °C, 30 °C, dan 40 °C. Angka peroksida sebagai produk primer dari oksidasi fillet ikan kakap meningkat 10,6 kali dengan kenaikan suhu dari 0 °C sampai 40 °C. Angka TBA meningkat 6,6 kali dari suhu 0 °C ke suhu 40 °C pada lama penyimpanan 45 hari.Kata kunci: Komposisi asam lemak; fillet ikan kakap (Lutjanus sp); angka peroksida; angka TB
Bioavailabilitas α-Tokoferol Minuman Emulsi Minyak Sawit dalam Plasma Darah dan Hati Tikus (Rattus norvegicus)
Get PDFTheoritically, it should contains high amount of α-tocopherol but its bioavailability have not been studied yet. The aim of this research were (1) to review the chemical composition of POED by doing proximate test then calculate α-tocopherol level in the product and (2) to evaluate bioavailability of α-tocopherol based on in vivo study. Bioavailability evaluation was carried out by measuring Relative Tocopherol Accumulation Factor (TAF), meanwhile its chemical composition was measured by proximate test and chromatographic analysis using HPLC. α-tocopherol contents of this products were 192,63 μg/g. Bioavailability study was carried out for 14 days using Rattus norvegicus rats. The prepared food contains 30 mg/kg α-tocopherol were given to vitamin-E-depleted rats. Tocopherol Accumulation Factor (TAF) proposed for the test group is 1/45,61, which indicated that 45,61 μg vitamin E from this product was needed to accumulate 1 μg tocopherol in the liver. Tocopherol relative bioavailability of POED was 82,46%. It showed that POED has good bioavailability.ABSTRAKMinuman emulsi minyak sawit (MEMS) merupakan salah produk berbasis emulsi oil-in-water yang dapat menjadi sumber vitamin E dalam bentuk α-tokoferol. Komposisi dasarnya adalah olein minyak sawit, air, dan pengemulsi. Secara teoritis, produk ini kaya akan α-tokoferol namun bioavailabilitasnya belum diketahui. Oleh karena itu, tujuan penelitian ini adalah (1) Menganalisis komposisi kimia MEMS melalui uji proksimat dan kadar α-tokoferolnya, dan (2) Mengevaluasi bagaimana bioavailabilitas α-tokoferol secara in vivo. Evaluasi bioavailabilitas dengan memperhitungkan Tocopherol Accumulation Factor (TAF) relatif, sedangkan komposisi kimia didapatkan melalui analisis proksimat dan kromatografi dengan HPLC untuk mengetahui kadar α-tokoferol. Kadar α-tokoferol yang didapatkan sebesar 192,63 μg/g. Pengujian biovailabilitas dilakukan selama 14 hari menggunakan hewan coba berupa tikus Rattus norvegicus. Ransum yang diberikan mengandung 30 mg/kg α-tokoferol. Nilai TAF sebesar 1/45,61, yang berarti bahwa 45,61 μg vitamin E dari MEMS dapat menghasilkan 1 μg tokoferol hati. Bioavailabilitas relatif α-tokoferol pada MEMS terhadap α-tokoferol murni sebesar 82,46%. Hasil tersebut menunjukkan bahwa MEMS memiliki bioavailabilitas yang baik.Kata kunci: α-tokoferol; bioavailabilitas; minyak sawit; minuman emulsi minyak sawit; Tocopherol Accumulation FactorTheoritically, it should contains high amount of α-tocopherol but its bioavailability have not been studied yet. The aim of this research were (1) to review the chemical composition of POED by doing proximate test then calculate α-tocopherol level in the product and (2) to evaluate bioavailability of α-tocopherol based on in vivo study. Bioavailability evaluation was carried out by measuring Relative Tocopherol Accumulation Factor (TAF), meanwhile its chemical composition was measured by proximate test and chromatographic analysis using HPLC. α-tocopherol contents of this products were 192,63 μg/g. Bioavailability study was carried out for 14 days using Rattus norvegicus rats. The prepared food contains 30 mg/kg α-tocopherol were given to vitamin-E-depleted rats. Tocopherol Accumulation Factor (TAF) proposed for the test group is 1/45,61, which indicated that 45,61 μg vitamin E from this product was needed to accumulate 1 μg tocopherol in the liver. Tocopherol relative bioavailability of POED was 82,46%. It showed that POED has good bioavailability.ABSTRAKMinuman emulsi minyak sawit (MEMS) merupakan salah produk berbasis emulsi oil-in-water yang dapat menjadi sumber vitamin E dalam bentuk α-tokoferol. Komposisi dasarnya adalah olein minyak sawit, air, dan pengemulsi. Secara teoritis, produk ini kaya akan α-tokoferol namun bioavailabilitasnya belum diketahui. Oleh karena itu, tujuan penelitian ini adalah (1) Menganalisis komposisi kimia MEMS melalui uji proksimat dan kadar α-tokoferolnya, dan (2) Mengevaluasi bagaimana bioavailabilitas α-tokoferol secara in vivo. Evaluasi bioavailabilitas dengan memperhitungkan Tocopherol Accumulation Factor (TAF) relatif, sedangkan komposisi kimia didapatkan melalui analisis proksimat dan kromatografi dengan HPLC untuk mengetahui kadar α-tokoferol. Kadar α-tokoferol yang didapatkan sebesar 192,63 μg/g. Pengujian biovailabilitas dilakukan selama 14 hari menggunakan hewan coba berupa tikus Rattus norvegicus. Ransum yang diberikan mengandung 30 mg/kg α-tokoferol. Nilai TAF sebesar 1/45,61, yang berarti bahwa 45,61 μg vitamin E dari MEMS dapat menghasilkan 1 μg tokoferol hati. Bioavailabilitas relatif α-tokoferol pada MEMS terhadap α-tokoferol murni sebesar 82,46%. Hasil tersebut menunjukkan bahwa MEMS memiliki bioavailabilitas yang baik.Kata kunci: α-tokoferol; bioavailabilitas; minyak sawit; minuman emulsi minyak sawit; Tocopherol Accumulation Facto
Model Matematis Hubungan antara Kadar Air Akhir Bahan dengan Variabel Proses Pengeringan pada Pneumatic Conveying Recirculated Dryer
Get PDFFlour drying could be conducted by using pneumatic conveying dryer (PCD) or flash dryer, but generally it is required a high vertical pipe. The high of vertical pipe may be replaced with a recirculation process to produce a required final moisture content of the material. This study had designed pneumatic conveying recirculated dryer (PCRD) to dryi of wet sago starch. Later, the design was used to determine a mathematical model of the relationship between the variables of drying process with final moisture content of the material. The purpose of this study was to develop a mathematical model of the relationship between the final moisture content of wet sago starch with variables drying process and recirculation continuously in the pneumatic conveying recirculated dryer (PCRD) using dimensional analysis. Buckingham Phi Theorem methods of dimensional analysis was used to find the relationship variables that affect the final moisture content of wet sago starch on the PCRD machine. The mathematical model generated in this study is The coefficient of determination (R2) of the mathematical model was 0.948, or 94.8 %, indicated that the model was valid to predict the final moisture content of wet sago starch in designing PCRD machines. While the sensitivity of the test results showed that the dimensionless product of the most influential are , , and . The model was applicable for drying wet sago starch or other starch material which is similat to the physical properties of wet sago starch. ABSTRAKPengeringan bahan-bahan tepung dapat dilakukan dengan pneumatic conveying dryer (PCD) atau flash dryer, namun umumnya memerlukan pipa vertikal yang cukup tinggi. Pipa vertikal yang tinggi dapat diganti dengan proses resirkulasi untuk menghasilkan kadar air akhir bahan yang disyaratkan. Pada penelitian ini telah dirancang pneumatic conveying recirculated dryer (PCRD) untuk mengeringkan pati sagu basah, serta dicari model hubungan matematis antara variabel-variabel proses pengeringan dengan kadar air akhir. Tujuan penelitian ini adalah mengembangkan model matematis hubungan antara kadar air akhir pati sagu basah dengan variabel-variabel proses pengeringan resirkulasi secara kontinyu pada pneumatic conveying recirculated dryer (PCRD) menggunakan analisis dimensi. Metode Buckingham Phi Theorem dalam analisis dimensi digunakan untuk mencari hubungan variabel-variabel yang berpengaruh terhadap kadar air akhir pati sagu basah pada mesin PCRD. Model matematis yang dihasilkan pada penelitian ini adalah Nilai koefisien determinasi (R2) dari model matematis tersebut adalah 0,948 atau 94,8 %, menunjukkan bahwa model tersebut valid digunakan untuk memprediksi kadar air akhir pati sagu basah dalam merancang mesin PCRD. Sedangkan hasil uji sensitivitas menunjukkan bahwa dimensionless product yang paling berpengaruh adalah , , dan . Model tersebut berlaku untuk pengeringan pati sagu basah atau bahan-bahan tepung lainnya yang sifat fisiknya identik dengan pati sagu basah.Flour drying can be done by using pneumatic conveying dryer (PCD) or flash dryer, but generally required a vertical pipe that is high enough. High vertical pipe can be replaced with a recirculation process to produce a required final moisture content of the material. This study had designed pneumatic conveying recirculated dryer (PCRD), and seek mathematical model of the relationship between the variables of drying process with final moisture content of the material. The purpose of this study is to develop a mathematical model of the relationship between the final moisture content of the material with variables drying process and recirculation continuously in the pneumatic conveying recirculated dryer (PCRD) using dimensional analysis. Buckingham Phi Theorem methods of dimensional analysis is used to find the relationship variables that affect the final moisture content of the material on the PCRD machine. The mathematical model generated in this study are The coefficient of determination (R2) of the mathematical model is 0.95, or 95 %, this indicates that the model is valid is used to predict the moisture content of the materials in designing PCRD machines. While the sensitivity of the test results showed that the dimensionless product of the most influential are , , and . The model is applicable for drying wet sago starch or other starch material are identical to the physical properties of wet sago starch.ABSTRAKPengeringan bahan-bahan tepung dapat dilakukan dengan pneumatic conveying dryer (PCD) atau flash dryer, namun umumnya memerlukan pipa vertikal yang cukup tinggi. Pipa vertikal yang tinggi dapat diganti dengan proses resirkulasi untuk menghasilkan kadar air akhir bahan yang disyaratkan. Pada penelitian ini telah dirancang pneumatic conveying recirculated dryer (PCRD), serta dicari model hubungan matematis antara variabel-variabel proses pengeringan dengan kadar air akhir bahan. Tujuan penelitian ini adalah mengembangkan model matematis hubungan antara kadar air akhir bahan dengan variabel-variabel proses pengeringan resirkulasi secara kontinyu pada pneumatic conveying recirculated dryer (PCRD) menggunakan analisis dimensi. Metode Buckingham Phi Theorem dalam analisis dimensi digunakan untuk mencari hubungan variabel-variabel yang berpengaruh terhadap kadar air akhir bahan pada mesin PCRD. Nilai koefisien determinasi (R2) dari model matematis tersebut adalah 0,95 atau 95%, hal ini menunjukkan bahwa model tersebut valid digunakan untuk memprediksi kadar air bahan dalam merancang mesin PCRD. Sedangkan hasil uji sensitivitas menunjukkan bahwa dimensionless product yang paling berpengaruh adalah , , dan . Model tersebut berlaku untuk pengeringan pati sagu basah atau bahan-bahan tepung lainnya yang sifat fisiknya identik dengan pati sagu basah