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UJI SITOTOKSISITAS DAN ANTIPROLIFERATIF FRAKSI ETIL ASETAT EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella sativa, Lour) TERHADAP SEL MIELOMA
Full text linkCancer is the formation of new tissue which is abnormal and malignant. A group of cells suddenly become disorganized and reduplicate themselves rigorously (hyperproliferation). Nigella sativa L. is one of the herbs which have an anticancer effect. This research aims to assess the cytotoxic and antiproliferative effect of Nigella sativa L. ethanol extract of Myeloma cells. Ethanolic extract was produced from Nigella sativa L. powder with maseration method. The cytotoxicity test was done by incubating Myeloma cells with the treatment concentration group of N. sativa L. ethyl acetic fraction of ethanolic extract 2000; 1000; 500; 250; and 62.5 µg/ml, respectively. The test was done with an MTT method and then with a calculation of its death percentage. The LC50 is calculated using a probit analysis method. The test was then continued with the antiproliferative test to assess the doubling time at treatment concentration 125; 62.5 µg/ml and cellular control at hours 24, 48, and 72. The results showed that Nigella sativa L. ethanolic extract had cytotoxic activity towards the Mieloma cells with an LC50 value 177.01 µg/ml. The antiproliferative test showed that there was a growth inhibition, even cell death at the extract treatments. The doubling time was 253 hours at 62.5 µg/ml concentration, 298.4 hours at 125 ug/ml, while the cell control had 54.52 hour
EFEK LIKOPEN TERHADAP GAMBARAN MIKROSKOPIK LIMPA TIKUS BETINA SPRAGUE DAWLEY
Full text linkCarotenoid compound, include licopene and lutein, has ability to increaseimmune system. One of organs included in immune system is spleen. The objective of thestudy is to observe the effect of licopene to the histopathologic appearance of ratspleen. Twenty female Sprague Dawley rat, 1 month old, 100 grams of weight devideinto 4 groups. The first group got licopene dosage 3 ug/kg of body weight, the secondgroup got licopene dosage 6 ug/kg of body weight, the third group got corn oil (vehiclecontrol) and the fourth group got aquadest. The treatment were given during 17 days,orally. At the day 14, the first, second and third groups were injected by aloxansubcutaneuslly. Three days after injection of aloxan, all of rats were sacrified, spleenwere taken in order to histopathological examination. Histopathologic appearances ofspleen were analized descriptivelly. The result showed that licopene dosage 6 ug/kg ofbody weigh able to increase centrum germinativum formation of spleen white pulp morethan licopene dosage 3 ug/kg of body weight. Base on the result can be concluded thatlicopene administration can increase centrum germinativum formation of spleen whitepulp
REAKSI PENATAAN ULANG FRIES PADA EUGENIL ASETAT
Full text linkFries Rearrangement of eugenyl acetate in order to study allilic substituentgroup influence has been done. Fries rearrangement of eugenyl acetate was done byheating at 120°C for 3 hours under AlCl3, dichloromethane as the catalyst and solvent,respectively. The structure of the compound was identified using Infra Redspectrometry (IR) and GC-MS. The result of te research showed that rearrangementfries of eugenyl acetate are not formed, but yielded lightly brown oil of dimmer
AKTIVITAS SITOTOKSIK ISOLAT 5 FRAKSI ETIL ASETAT EKSTRAK PETROLEUM ETER DAUN Phaleria macrocarpa (Scheff.) Boerl. PADA TURUNAN SEL KANKER SERVIKS MANUSIA (HeLa)
Full text linkCervical cancer is a malignant tumor in cervix which is occupy the second possition that cause the death of Indonesia’s women under the breast cancer. One of the traditional medicine that can be used to cure cancer is Phaleria macrocarpa (Scheff.)Boerl leaves. The petroleum eter extract of Phaleria macrocarpa (Scheff.)Boerl leaves has antiproliferation activity againts cervical cancer cell lines (HeLa) with IC50 9 µg/ml (Kintoko & Azimahtol Hawariah, 2007). This research was aimed to detect cytotoxic activity of isolate 5 of ethyl acetate fraction of petroleum ether extract of Phaleria macrocarpa (Scheff.)Boerl leaves againts HeLa cell lines. Initially, the powder of Phaleria macrocarpa (Scheff.)Boerl was extracted using Soxhlet apparatus with petroleum ether. The extracts was concentrated and partitioned until ethyl acetate fraction was obtained. The fraction of ethyl acetate was isolated using Preparative Thin Layer Chromatography on silica gel F254 as stationary phase and n-hexane : ethyl acetate (9 : 1) as mobile phase. Cytotoxic activity against HeLa cell lines was determinated by LC50 value by direct counting. The isolation by preparative thin layer chromatography resulted five isolate on Rf value : 0,05 ; 0,10 ; 0,20 ; 0,60 ; and 0,90. The LC50 values of isolate 5 (Rf : 0,90) was 123,60 µg/ml
EFEK HEPATOPROTEKTIF EKSTRAK METANOL JAHE MERAH (Zingiber officinale Roscoe) PADA MENCIT JANTAN YANG DIINDUKSI CCl4
Full text linkMeOH extract of Zingiber officinale Roscoe (ZO) were investigated for hepatoprotective effect at the doses (p.o) of 100 and 200 mg/kg body weight using CCl4 induction method. Male Swiss mouse (25±1,5 g) were treated with ZO doses 100 and 200 mg/kg bw and Sillymarin (100 mg/kg bw) for two week before treatment with a single dose of CCl4, 0.5 ml/kg bw, intraperitoneally. The mouse were sacrifactivity of SGPT-SGOT. The study showed the activity of SGPT of MeOH extract of ZO doses 100 mg/kg bw and 200 mg/kg bw, Sillymarin, control and normal groups are (34.51±5.34), (27.38±4.28), (27.26±1.06), (36.61±1.87), and (25.37±2.01) U/L, respectively. While the activity of SGOT of MeOH extract of ZO doses 100 mg/kg bw and 200 mg/kg bw, Sillymarin, control and normal groups are (88.88±0.94), (83.88±3.91), (83.99±7.24), (92.97±8.16), and (82.99±3.97) U/L, respectively. MeOH extract of ZO dose 200 mg/kg BW and Sillymarin significantly (p < 0,05) decrease the activities two enzymes (SGPT and SGOT). The results showed MeOH extract Zingiber officinale Roxb has hepatoprotective effec
ANALISIS SGPT-SGOT EKSTRAK ETANOL DAGING BUAH PARE (Momordica charantia L.) PADA TIKUS JANTAN PUTIH GALUR WISTAR
Full text linkThe traditional medicine in Indonesia which is the cultural heritage and hasbecome an integral part of the life of the nation Indonesia, want to be employed in theformal health care system. As the circulation of drugs in general terms, traditionalmedicine should fulfill the requirements of quality, safe, and efficacious. This studyaims to analyze the levels of SGPT, SGOT of suspension of the ethanol extract of bittermelon fruit (Momordica charantia L.) by orally. The study was conducted using a whitemale Wistar rats amounted to 36 animals, divided into 6 groups. Each group consistedof 6 male white rats. Group I (control) were given 0.5% CMC solution of 2.5 ml/200 gBW. Group II was given a suspension dosage doses of bitter melon fruit extract ethanol0.5 g/kg, then consecutive group III with the test preparation doses of 1 g/kg, the testpreparation group IV were given a dose of 2 g/kg, group V was given dosage test doseof 4 g/kg, and group VI were given the highest dose of the test preparation 8 g/kg. Theresults of acute toxicity tests on white male Wistar rats showed that administration ofethanol extract of peroral dosage suspension pare (Momordica charantia L.) singledose on white male Wistar rats from the dose of 0.5 g/kg until a dose of 8 g/kg did notsignificantly influence the value of SGPT, SGOT