Journal of Tropical Biodiversity and Biotechnology
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Isolation and Characterization of Lactic Acid Bacteria from Inasua
Get PDFInasua is a traditionally product of wet salt fish fermentation produced by Teon, Nila and Serua (TNS) Communities in Central Maluku, Indonesia. The community made this fermented fish to anticipate the lean time when fisherman could not go to sea. The fish that used as inasua raw material is demersal fishes that live around coral reefs, such as Samandar fish (Siganatus guttatus), Gala-gala fish (Lutjanus sp.) and Sikuda fish (Lethrinus ornatus). The objective of the research was to isolate and characterize of bacterial indigenous in Inasua from three producers in Seram Island. The measurement of pH from inasua samples were 5.9, 5.0 and 5.8, respectively. The highest number of lactic acid bacteria was found from Gala – gala inasua was 2,5x107 cfu/g sample. Isolation of all isolates bacteria from inasua showed that a total of 7 isolates of bacteria was obtained from Samadar inasua, 9 isolates from Gala-gala inasua, and 7 isolates from Sikuda inasua. From a total of 23 isolates, only 6 isolates had characteristic as lactic acid bacteria that were Gram positive, negative catalase, and cocci shape. The microscopic characteristics of the isolates are coccid in pairs or uniforms which combine to form tetrads. Carbohydrate utilization test of selected isolate by using API 50 CHB kit indicated that 13 carbohydrates are fermented by these isolates after incubation for 48 hours. The research was concluded that the dominant bacteria in inasua sample is cocci-lactic acid bacteria.Keywords : fermented fish, inasua, lactic acid bacteria, MRSA mediu
Potensi Aktinomisetes Asal Tanah Perakaran Mangrove Segara Anakan Cilacap Sebagai Penghasil Antifungi Terhadap Yeast Patogen Candida albicans
No full textIsolation of actinomycetes has been done from mangrove soil sample of Segara Anakan Cilacap. This research aimed to know the potency of actinomycetes as an antifungal producer, and to measure antifungal activity produced by actinomycetes based on diameter zone inhibition and to know the characteristic of the bioactive compound with Thin Layer Chromatography method. The result of the study has been isolated 24 actinomycetes isolates, 15 of them had potential as an antifungal producer, which inhibited the growth of Candida albicans. Period of fermentation significantly affected to the activity of the antifungal compound. The highest inhibitory zone was formed by an antifungal extract from actinomycetes C with a range zone 19.7 mm in length at 14th days fermentation, which was extracted with ethyl acetate solvent (1: 1 v / v). The characteristic of a bioactive compound of the actinomycetes C ha s a Rf value 0.5 in the solvent n-butanol, acetic acid and water (3: 2: 1 v / v / v)
Spesies Tumbuhan Penyusun Vegetasi Lantai di Wilayah Restorasi Taman Nasional Gunung Merapi di Ngablak, Magelang, Jawa Tengah
Get PDFVegetation restoration by Mount Merapi National Park (TNGM) in Ngablak, Magelang, Central Java need the floristic data composition of understory vegetation. The objectives of this research were to identify and to determine the importance values of each plants species that composed of that restoration area. Sample collections were conducted using survey method for their morphological characters as voucher specimens that were used for species identification. Vegetation analysis was conducted using the quadratic method; shrubs (5x5 m2), grasses and herbs (1x1 m2), and importance values were analysed using relative frequencies and densities. The result shows that understory vegetation on the restoration area in Ngablak was composed of 60 seed plant species from 18 plant families. There were 7 plant families that dominate the region; Papilionaceae (13 species), Asteraceae (11), Poaceae (Grasses) (10), Cyperaceae (Sedges) (5), Amaranthaceae (3), Mimosaceae (3) and Commelinaceae (3). Plants species that have important value more than 25% in this restoration area were Cymbopogon citratus (sereh), Mimosa pudica (Putri malu), Gomphrena serrata (Bunga kancing), Pannisetum purpureum (Rumput gajah), Ageratum conyzoides (Wedusan), dan Euphatorium inulifolium (kirinyuh). The external factors that influence the growth of the understory vegetation are pH of soil and soil moisture
The Effect Of Cadmium on Sperm Quality and Fertilization Of Cyprinus carpio L.
Get PDFThe objective of the study was to determine the effect of cadmium on sperm quality and fertilization of C. carpio L. Sperm and eggs were collected by abdomen striping from the mature testis and ovary of C. carpio L. This study used one control and four treatment groups of variation on the cadmium concentration (0, 50, 100, 150, and 200 ppm) with eight replications. Sperm motility (mass motility, mass motility duration, and individual motility duration) and viability were measured after three to four seconds of incubation in the water. The percentage of fertility success was calculated by observing embryo development after the eggs were mixed with sperm and incubated in the water for 72 hours. The success of the fertilization process was indicated by a color change of the egg that darkens after successful fertilization, and white-milk if failed. The data were analyzed using analysis of variance (α = 0.05). The results of this study indicate that exposure of 50 ppm cadmium and control group shown success in term of sperm quality (motility and viability) and fertilization, but at 100 ppm or more decreased the sperm quality and fertilization rate. It can be concluded that cadmium exposure decreases sperm quality and fertility at 100 ppm or higher concentrations
Identifikasi Bakteri Pada Serasah Daun Mangrove yang Terdekomposisi di Kawasan Konservasi Mangrove dan Bekantan (KKMB) Kota Tarakan
Get PDFBacteria in mangrove ecosystem has a significant role in litter decomposition that is essential to provide organic nutrition for organisms in mangrove forest. The research was aimed to identify decomposition bacteria that exist in KKMB Tarakan mangrove conservation area. The identification focused on litters from several mangrove vegetation, which are Bruguiera parviflora, Rhizophora apiculata, Sonneratia alba, dan Avicennia alba. The method used was explorative descriptive while testing parameter was genus. The research identified seven bacteria genera for litter from Bruguiera parviflora which are Bacillus, Aeromonas, Nocardiae, Corynebacterium, Listeria, Bacteroides, Pseudomonas. Six genera from Rhizophora apiculata which are Bacillus, Listeria, Enterobacteria, Aeromonas, Actinobacilus, and Bacteroides. Five genera for Sonneratia alba which are Bacillus, Aeromonas, Listeria, Enterobacteria, Staphylococcus. Also eight genera from Avicennia alba which are Bacillus, Clostridium, Enterobacteria, Bacteroides, Plesiomonas, Bordella, Streptococcus, and Neisseria. The results conclude that different species of mangrove vegetation attracts different species of bacteria as well
Kandungan Fenolik, Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Paku Laut (Acrostichum aureum L.) Fertil dan Steril di Kawasan Mangrove Kulon Progo, Yogyakarta
No full textSea Fern (Acrostichum aureum L.), which typically grows in mangrove ecosystem, has two distinguished leaves which are fertile leaves (rich in spores) and sterile leaves (no spores). Those leaves are known rich in phenolic compounds. Flavonoids, moreover, which belong to class of phenolic compound, have antioxidant activity. This study aims were to analyze the phenolic content, flavonoid content, and antioxidant activity of fertile leaves, sterile leaves, and spores of sea fern which grow in mangrove ecosystem that is located in Kulon Progo, Yogyakarta. Leaf and spore samples were extracted using ethanol solvent. Then, total content of phenol and flavonoid were measured by spectrophotometry, while the antioxidant activity was measured by DPPH method. The results showed that the phenolic content of fertilized leaves and sterile leaves did not show a significant difference. In other hand, the spores sample showed highest both of flavonoids content (2.32 mg/mL) and the antioxidant activity with the IC50 value was 23.25 ppm. Thus, the ethanolic extracts of spores of sea fern sterile leaves showed the highest flavonoid content and could be categorized as a very strong antioxidant
Moluska Benthik di Perairan Lima Muara Sungai Kawasan Teluk Lamong, Surabaya, Jawa Timur
Get PDFObservation on mollusk on five estuary of Lamong Bay waters was conducted on June and October 2005. The aim of this study was to determine condition and community structure of molluscs living in those areas. Samples were collected using Eckmann grab and sieved through 0.5 mm mesh-sized. The number of mollusk species was 21 species, consist of 8 species class of Gastropoda and 13 species class of Bivalvia. A diversity index (H) ranged 0.802 to 1.028, an evenness index (J) was 0.923 to 1 and a richness index (D) was 15.126 to 20.959. In which Tellina palatum (Tellinidae) and Placemen calophyla (Veneridae) class of Bivalvia relatively wide distributed. In generally, this result showed that five estuary of Lamong Bay waters has a relatively low molluscs diversity
Variasi Morfologis Udang Galah (Macrobrachium rosenbergii de Man, 1879) Hasil Inbreeding dan Outbreeding Populasi Probolinggo dan Mahakam
Get PDFIndonesia has high biodiversity, one of them is the diversity of giant freshwater prawn (Macrobrachium rosenbergii de Man, 1879). The giant freshwater prawn was easy to reproduce and has high nutritional value. This study was conducted to determine the morphological variation of inbreed and outbreed of Probolinggo and Mahakam (Mama, Gogo, Goma, and Mago) populations that was developed in Unit Kerja Budidaya Air Payau Balai Budidaya Udang Galah (UKBAP BBUG) Samas as a basic for seed selection. Research of morphological variation carried out by observing 12 morphological characters, four meristic characters and 52 morphometric characters in four populations. Analysis of basic morphometric characters using a statistical program to determine the linear regression and the significance of parameters. Analysis of morphological characters using a statistical program to make dendrogram showing the similarity of four populations. The result of morphometry shows that the ratio of cephalothorax and abdomen of Mago and Goma is lower than Mama and Gogo. When viewed from the ratio of the carapace and rostrum length, Mago produces the longest rostrum. So, when only carapace length is compared to abdomen length, Mago showed the greatest value. Gogo has the highest ratio of cephalothorax and abdomen, while Mago has the highest ratio of carapace and abdomen among populations. Morphological data analysis showed that Gogo and Goma had reached 76% similarity, Goma and Mama have a similarity of 68% while Mago has 52% similarity with another three populations
Bioaktivitas ekstrak batang Xylocarpus granatum sebagai anti black spot alternatif pada Litopenaeus vannamei pasca panen
Get PDFThis research was done to explore bioactivity of Xylocarpus granatum’s stem extract as tyrosinase inhibitor for alternative preventing black spot in Litopenaeus vannamei postharvest. In this research, extraction of bioactive compound from X. granatum stem was done by soaking the stem in 96% methanol for 24h at temperature 29°C. The methanol was evaporated by using rotary evaporator at temperature 50°C. The extract was dilute with distillate water at series of dilutions (10%, 30%, 50%, 70%, 90%, and distillate water as the control), and then immersing L. vannamei into X. granatum’s stem extract as much as 10ml for 6h, and finally the data analyzing use the Kruskal Wallis test and the Least Significant Difference (LSD) test with the degree of confident 95%. The result showed that X. granatum’s stem extract is able to prevent a black spot of L. vannamei after harvesting process. Furthermore, concentration of 10 % of extract is an effective concentration for preventing a black spot and the control has black spot 0.0360 – 0.0373 mm2
Biomass Production of Root and Shoot of Talinum paniculatum Gaertn. by Liquid and Solid Ms Medium with Plant Growth Hormone IBA
Get PDFTalinum paniculatum Gaertn. is one of traditional medicinal plant in Indonesia which has benefits such as for vitality and maintain blood circulation. The aim of this research is to obtain biomass production of root and shoot of T. paniculatum Gaertn. by liquid and solid MS medium with IBA. This research conducted to provide biomass as raw material for secondary metabolites test. Stems as explant were induced with four treatments (liquid MS, solid MS, liquid MS + 2 ppm IBA and solid MS + 2 ppm IBA) with five repetitions. Observation did for 28 days. The parameters are the percentage of explants which formed the root and shoot, morphology, fresh and dry biomass. Result shows that percentage of root and shoot have 100% in liquid and solid MS + 2 ppm IBA. Fresh and dry biomass of root and shoot in solid MS + 2 ppm IBA higher than the others. This research found callus in liquid and solid MS + 2 ppm IBA. Morphology of root in liquid MS has thin and friable, but thick in solid MS. Shoot in solid and liquid MS has thin, short and sturdy