Majalah Obat Tradisional
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Screening and Prediction of Potential Compounds from Virgin Olive Oil Acting on Proteins Associated with Cancer Disease
Get PDFVirgin olive oil contains phenolic compounds that were potential for anti-inflammatory and cancer treatment. Computational biology is a beneficial method to understand how this compound can affect the biological process in humans. This research is conducted by the potential screening of VOO compounds, constructing the pharmacological network and enrichment, and docking simulation. The enrichment result showed that the EGFR, BRAF, MAPK1, CCND1, and MDM2 protein have multiple cancer contributions and related pathways. The docking simulation result showed that the interaction of EGFR-luteolin, BRAF-luteolin, MAPK1-luteolin, CCND1-apigenin, and MDM2-1-hydroxypinoresinol has the highest binding affinity. Further research with the in-vitro method is required to check the reliable mechanisms of each compound to their protein target
Ethanolic Extract of Pluchea indica Less leaf Increases Serum Growth Hormone in Lactating Rats
Get PDFThe objectives of the study were to evaluate the effect of ethanolic extract of P. indica Less leaf (EPI) on serum growth hormone (GH), milk yield, body weight gain of dams, and dam’s organ weight in lactating rats. A total of 25 lactating rats with six pups were randomized and distributed to one of five treatments (control (RO water), standard (domperidone 2,5 mg/kg BW/day), EPI 250 (250 mg/kg BW of EPI), EPI 500 (500 mg/kg BW of EPI), and EPI 750 (750 mg/kg BW of EPI)). The treatment was administered daily starting from the 2nd until the 15th day of lactation. On the 16th day serum growth hormone level, body and organ weight of rats were measured. Serum GH levels in the EPI 750 group (1963.25 ± 360.91 pg/µL) increased significantly compared to the domperidone (409.46 ± 28.80 pg/µL) and the control group (723.40 ± 95.78 pg/µL, p0.05). There was significant body weight gain in the EPI 750 group compared with the domperidone group. There was no significant difference in organ weight gain in each group. The study revealed that ethanolic extract of P. indica Less leaf can increase serum growth hormone in lactating rats
In Silico Study on the Effect of Heliannuol A, B, C, D, E Compounds of Sunflower (Helianthus annuus L.) on Dual PI3K/mTOR (5OQ4) Enzyme
Get PDFHeliannuol is a sesquiterpene that has a benzoxepine ring, oxepin. Many derivatives of benzoxepine compounds show anticancer activity by inhibiting the phosphoinositide 3-kinase (PI3K) enzyme. These enzymes play a role in cell proliferation and growth. The study aims to predict the physicochemical properties using Lipinski’s Rule of Five parameters on phosphoinositide 3- kinase (PI3K/Mtor; PDB 5OQ4) enzyme and the toxicity of Heliannuol A, B, C, D, E compounds. The process uses the pkCSM online tool. The validation of receptor 5OQ4 is done using the value parameter RMSD < 2 (Å). Protox online tool dan pkCSM online tool is employed to predict the toxicity using parameter LD50, skin sensitization, Ames toxicity, hepatotoxicity, and toxicity class. The interaction of ligan and enzyme is tested using Molegro Virtual Docker 6.0. Heliannoul A, B, C, D, E compounds fulfill Lipinski’s Rule of Five. The receptor 5OQ4 is known valid using the value of RMSD 0,923 (Å). Heliannuol A, B, C, D, E compounds inhibit Dual PI3K / mTOR enzyme less than Bimiralisib. As a result of the toxicity test of compounds Helliannouls A, B, C, E, and Bimiralisib compounds are included in class 4, while Helliannouls D compounds are included in class 5
Application of Ozonated Olive Oil as Adjunctive Therapy after Periodontal Pocket Curettage towards Collagen Density of Alveolar Bone in Periodontitis Healing Process (In Vivo Study with Sprague dawley)
Get PDFPeriodontitis is an inflammatory disease of the supporting tissue of the teeth caused by specific microorganisms or groups of specific microorganisms, resulting in progressive destruction of the periodontal ligament and alveolar bone with periodontal pocket formation. Application of ozonated olive oil in dentistry is based on the actions such as antimicrobial and therapeutic agent, needed as adjunctive therapy after periodontal pocket curretage. Collagen is the main constituent of alveolar bone extracellular matrix and needed as a scaffold in the formation of mineralized matrix. The aim of this study was to determine the density of collagen in alveolar bone on periodontitis healing process after adjunctive topical application of ozonated olive oil in periodontal pocket curretage. In this study, 32 Sprague dawley rats were randomly divided into two groups: curetted-topical application of ozonated olive oil as treatment groups and curetted-topical application of 1% CMC-Na as placebo group. Periodontitis induced by placing silk-ligature around submandibular incisors for 7 days. Subsequently, the rats were sacrificed on days 3, 5, 7, and 14 after curetted and topical application, and each group was represented by four rats. The staining was done using Mallory staining method. All the results were statistically analyzed using Kruskal-Wallis and Mann-Whitney tests. The results showed that there were significant differences (p<0,05) in the density of collagen between two groups. The study concluded that adjunctive topical application of ozonated olive oil after periodontal pocket curretage significantly increase the density of collagen in alveolar bone on periodontitis healing process in Spraque dawley
Antioxidants and Antityrosinase Activity of Ethanolic Basil Leaves Extract (Ocimum americanum L.) and Eygenol
Get PDFHarm effect from UV light, both UV-A and UV-B is contributing as a disease trigger and has an impact on human health. To investigate the bioactive compound, antioxidant, and the potential of basil leaves as antiaging sources particularly as the tyrosinase inhibitor. This study used phytochemical screening for the bioactive compound, DPPH scavenging activity for antioxidant assay, and tyrosinase inhibition activity for the antiaging property. The phytochemical screening shows that the basil leaves extract has flavonoid, saponin, phenol, steroid, and alkaloid. The basil leaves extract has lower antioxidant activity (20.55 ± 0.04 μg/mL) compared with eugenol (2.44 ± 0.26 μg/mL) through DPPH scavenging activity. The basil leaves extract (35.59 ± 0.83 μg/mL) has lower antiaging activity particularly as antityrosinase activity compared with eugenol (10.87 ± 0.41 μg/mL). Our findings suggest that basil leaves can be used as an antioxidant and antiaging source, particularly as a tyrosinase inhibitor
The Extract of Purple Sweet Potato Against Heat-Shock Protein 70 Expression in White Male Rat of Atherosclerosis Model
No full textShock protein 70 (Hsp-70) molecule is pro-inflammatory mediator cytokine that trigger atherosclerosis. The purple sweet potato has many natural antioxidants such as flavonoids (anthocyanins) and is valuable for reducing Hsp-70 expression due to its antioxidant content.This study aims to determine the effect of purple sweet potato (Ipoema batatas L.) extract in reducing the Hsp-70 levels in the white male rat atherosclerosis model.This study was a posttest-only control group design with normality, homogeneity, ANOVA, post hoc test, correlation, and regression tests. There were five groups in this study. Groups 1 (negative control) and 2 (positive control) were given 2 ml/day of high-cholesterol diet for eight weeks, and the other three groups were given purple sweet potato extract at 24, 48, and 96 mg/kg doses weight per day for eight weeks. Results: The purple sweet potato extract affected the Hsp-70 levels in Wistar strain rats (p < 0.05). There was a significant difference in the Hsp-70 level between the positive control group and the group with purple sweet potato extract. The highest Hsp-70 level reduction was seen in the group with a 96 mg/day dose of purple sweet potato extract. In conclusion, theadministration of purple sweet potato extract (I. batatas L.) reduced the Hsp-70 levels. The dose of 96 mg/kg BW/day had the highest effect on decreasing Hsp-70 levels in the male rat atherosclerosis model
Supplementation of Garcinia mangostana Linn and Vasconcellea pubescens A.DC extract reduced exercise-induced oxidative stress in rats
Get PDFThe objective of this study was to see how supplementation with Garcinia mangostana extract (GME) and Vasconcellea pubescens extract (VPE) affected exercise performance and oxidative stress in intensely exercised rats. Twenty-five male rats were used in the study. Rats were divided into five groups: normal, stress oxidative control, combination GME:VPE (50:50), combination GME:VPE (75:25), and combination GME:VPE (25:75) groups. The antioxidant activity of a single extract from the literature was used to make the percentage comparison between extracts. The chronic exercise was done by swimming at 10 m/day for two weeks. Rats in the acute exercise groups were treated by swimming on the pail containing water. Blood samples were collected from the orbital sinus to determine hematological parameters. Liver tissue samples for the analysis of malondialdehyde (MDA) and glutathione (GPx) markers. Data analysis was performed statistically using One Way ANOVA. The combination of GME and VPE was shown to be effective in reducing oxidative stress and increasing MDA and GPx enzyme activity. The administration of both extracts also showed changes in the hematological profile
Cytotoxicity and Antiobesity Activity of Freeze-Dried Malus domestica, Canarium sp. and Averrhoa bilimbi Fruit
Get PDFObesity has a role in the development of diseases such as diabetes, cardiovascular disease and hyperlipidemia which is characterized by the increase of adipose tissue mass due to an imbalance of energy intake and expenditure. Freeze-dried fruits are well known to possess antiobesity activity. In this study, we have evaluated the antiobesity activity of freeze-dried fruit (M. domestica, Canarium sp., and A. bilimbi) using CHOL, G6PDH, TG level, and Oil Red O assay. The viability of 3T3-L1 cell in the Canarium sp. freeze-dried in the concentration of 12.50 µg/ml has a higher value compared to M. domestica and A. bilimbi. The measurements of CHOL, G6PDH, TG level, and Oil Red O assay of the M. domestica freeze-dried in the concentration of 75 μg/ml has higher inhibitory activity compared to the Canarium sp. and A. bilimbi freeze-dried. In the CHOL assay, M. domestica freeze-dried has a higher value compared to A. bilimbi and Canarium sp. In the G6PDH assay, the freeze-dried of M. domestica has the value of 49.56%, Canarium sp. (45.22%), and A. bilimbi (47.13%), while in the Oil Red O assay, M. domestica has inhibition activity of 62.63%, A. bilimbi 50.01% and Canarium sp. 44.13%. The level of TG showed that M. domestica has higher activity with the value of 60.61%, A. bilimbi 57.54%, while Canarium sp. 55.03%. The freeze-dried of M. domestica in the concentration of 75 μg/ml has good inhibitory activity of lipid compared to A. bilimbi and Canarium sp
The Antioxidant Capacity of Peristrophe Bivalvis (L.) Merr. as Natural-Based Nephroprotection
Get PDFThe kidneys as one of the important body organs have a very important role in maintaining a healthy body. The kidneys function to regulate fluid balance in the body the concentration of salt in the blood, acid-base balance in the blood, and excretion of waste materials such as urea and other nitrogenous waste in the blood. Magenta plants (Peristrophe bivalvis (L.) Merr.) contain secondary metabolites, namely alkaloids, saponins, triterpenoids, tannins, and flavonoids as antioxidants. The abundance of antioxidants sourced from natural sources for various diseases is often used as a complementary therapy and is one of the current therapeutic choices. However, the development of natural sources must also consider kidney function during an intervention. The incidence of kidney failure can be caused either by the occurrence of oxidative stress or exposure to drugs and other chemical compounds must also consider the physiological functions of important organs in the body such as the liver and kidneys. This study was conducted to determine the protective role of magenta leaves extract (Peristrophe bivalvis (L.) Merr.) on the kidneys after being given an acetaminophen hepatotoxic dose. In this study, the effectiveness of magenta leaves antioxidants and the safety of use was analyzed by looking at the kidney function in the experimental model of Wistar strain male white rats, using a Randomized Post Test Only Control Group Design. Four treatment groups showed that magenta leaves extract (Peristrophe bivalvis (L.) Merr.) at 125 and 250 mg/kg BW can protect the kidneys with average creatinine levels of 0.63 and 0.75 and with a normal range (0.7 - 1.2). It means that these two groups could protect the kidney function although in the histopathology test only the group administering extracts of 250 mg/Kg BW showed good results. It can be concluded that administration of the magenta leaves extracts at 250 mg/kg BW can protect renal function as seen from serum creatinine levels. Besides, histopathological features can provide a protective effect on the kidneys with the incidence of necrosis in the kidneys of less than 60% of the toxic dose of acetaminophen
Sunscreen Activity of Fraction n-hexane, Chloroform, and Eethyl Acetate of Ethanol 96% Flamboyan Leaf (Delonix regia. Raf) Extract
Get PDFThe sunscreen test of fraction n-hexane, chloroform, and ethyl acetate of ethanol 96% flamboyant leaf (Delonix regia. Raf) extract had been performed. This research begins the extraction of a flamboyant leaf using 96% ethanol. Extraction used the maceration method. The extract is fractionation with some solvents such as n-hexane, chloroform, and ethyl acetate. Each fraction was identified secondary metabolite and sunscreen test involving SPF, %Te, and %Tp measurement using UV-Vis Spectrophotometer. The result of phytochemical screening exhibited flavonoids, alkaloids, saponins, tannins, and phenolics in ethyl acetate fraction. n-hexane and chloroform fraction don't show saponins and flavonoid content. The sunscreen test shows that chloroform fraction has good protection power toward UV light with SPF, %Te, and %Tp value is 54.27±0.462, 7.46±0.473, and 12.83±0.047 in 250 mg/L, respectively