Jurnal Fitopatologi Indonesia
Not a member yet
412 research outputs found
Sort by
Combination of Barrier Crop, Chitosan, Bougaivillea Leaf Extract to Manage Virus on Soybean
Get PDFInfeksi virus menjadi salah satu pembatas produksi kedelai di Indonesia. Penelitian bertujuan menguji efektivitas kitosan, ekstrak daun bugenvil, dan kombinasinya, serta insektisida dalam mengendalikan infeksi virus pada kedelai di lapangan. Efektivitas perlakuan diuji dalam petak utama tanpa dan dengan pembatas jagung. Peubah penyakit, populasi serangga, dan peubah agronomi diamati mingguan. Populasi kutudaun pada perlakuan kontrol dengan tanaman pembatas lebih rendah daripada perlakuan kontrol tanpa tanaman pembatas, menunjukkan tanaman pembatas mampu menghalangi kutudaun masuk ke pertanaman kedelai. Inkorporasi perlakuan kitosan, ekstrak daun bugenvil, dan kombinasinya mampu menekan populasi kutudaun sebanding dengan perlakuan insektisida pada kedua petak utama. Area under population progress curve (AUPPC) kutudaun selaras dengan lebih rendahnya area under disease progress curve (AUDPC), insidensi dan keparahan penyakit serta AUDPC secara nyata dibandingkan dengan kontrol tanpa perlakuan. Efektivitas perlakuan pada petak dengan tanaman pembatas mampu menurunkan populasi kutudaun, insidensi dan keparahan penyakit lebih tinggi dibandingkan dengan perlakuan yang sama pada petak tanpa tanaman pembatas. AUDPC antarperlakuan kitosan, ekstrak daun bugenvil, kombinasi, dan insektisida secara umum berbeda nyata lebih rendah pada kedua petak utama dibandingkan dengan kontrol tanpa perlakuan. Hasil panen kedelai menunjukkan lebih tinggi secara signifikan daripada perlakuan kitosan, ekstrak daun bugenvil, dan kombinasinya dibandingkan dengan perlakuan kontrol pada kedua petak utama. Penggunaan tanaman pembatas yang disatukan dengan salah satu perlakuan kitosan dan ekstrak daun bugenvil dapat menjadi paket pengelolaan yang ramah lingkungan untuk mengendalikan infeksi virus pada tanaman kedelai di lapangan.Combination of Barrier Crop, Chitosan, Bougaivillea Leaf Extract to Manage Virus on Soybean
Viral infection is one of the limiting factor of soybean production in Indonesia. This study aimed to test the effectiveness of chitosan, bougainvillea leaf extract, and their combinations as well as insecticide as comparison treatment in controlling virus infections on soybeans in the field. The effectiveness of treatments were tested in main plot with and without maize as barrier plants. Disease assessment, insect population, and agronomic variables were observed weekly. The aphid population in the control treatment with barrier plants showed significantly lower than control without barrier plants, indicating that barrier plants able to hindered aphids to enter the soybean crops. Incorporating of chitosan, bougainvillea leaf extract, and their combination treatments able to control aphid population comparable to insecticide treatment in both main plots. The low AUPPC of aphid populations were in line with lower disease incidence, severity in compared to AUDPC of control treatments. The effectiveness of treatments in plot with barrier plants able to reduced aphid population and disease intensity higher than similar treatments in main plot without barrier plants. The AUDPC among chitosan, bougainvillea leaf extract, combination, and insecticide treatments was significantly lower in both main plots in compared to control treatment. Soybean yields showed significantly higher in chitosan, bougainvillea leaf extract, and combination treatment than control treatments in both main plots. The utilization of maize as barrier plants incorporated by one of chitosan and/or bougainvillea leaf extract will become a eco-friendly management package to control viruses infecting soybean in the field
Population of Soil Nematodes in The Treatment of Brassicaceae Plant Waste
Get PDFTanaman famili Brassicaceae diketahui mengandung glukosinolat yang dapat terhidrolisis menjadi senyawa yang bersifat biofumigan. Penelitian ini dilakukan untuk menguji pengaruh biofumigasi limbah tanaman Brassicaceae terhadap dinamika populasi nematoda tanah. Percobaan rumah kaca dilakukan pada empat jenis limbah tanaman Brassicaceae, yaitu limbah daun lobak (Raphanus sativus), brokoli (Brassica oleracea var. italica), kubis (Brassica oleracea var. capitata), dan seluruh bagian gulma kamanilan (Roripa indica) dalam pot berisi 5 L tanah terinfestasi nematoda. Limbah tanaman dicacah berukuran ±1 cm, sebanyak 117 g per pot dicampurkan dalam tanah terinfestasi nematoda, disiram air hingga basah, dan ditutup rapat untuk proses biofumigasi selama 14 hari. Pot dibiarkan terbuka selama 3-5 hari, ditanami bibit mentimun varietas Roberto 92 berumur 7 hari, dan dipelihara di rumah kaca hingga 8 minggu setelah tanam. Pengamatan jenis dan populasi nematoda tanah dilakukan tiga kali, yaitu sebelum perlakuan, setelah perlakuan, dan 8 minggu setelah tanam. Percobaan ini disusun dalam rancangan acak lengkap dengan 6 perlakuan dan 5 ulangan. Hasil penelitian menunjukkan terjadi peningkatan nematoda free-living (nematoda bakteriovora dan nematoda fungivora) pada seluruh perlakuan limbah tanaman Brassicaceae. Sebaliknya, terjadi penurunan populasi fitonematoda secara nyata pada perlakuan limbah daun brokoli. Semua jenis limbah tanaman Brassicaceae dapat menekan populasi fitonematoda Helicotylenchus sp., Rotylenchulus sp., dan Xiphinema sp., secara nyata. Limbah daun brokoli menunjukkan nilai penghambatan tertinggi terhadap seluruh genus fitonematoda dengan penghambatan mencapai 100%.Population of Soil Nematodes in The Treatment of Brassicaceae Plant Waste
Brassicaceae plants are known to contain glucosinolates which can be hydrolyzed into biofumigant compounds. This research was conducted to examine the effect of biofumigant derived from Brassicaceae plants waste on soil nematode population dynamics. Greenhouse experiment was carried out on 4 types of Brassicaceae plants waste, consisting of leaf waste of radish (Raphanus sativus), broccoli (Brassica oleracea var. italica), cabbage (Brassica oleracea var. capitata), and whole parts kamanilan weed (Roripa indica) in polybags containing 5 L of nematode infested soil. Plant waste was chopped ± 1 cm in size, then as much as 117 g per polybag was mixed in nematode infested soil, doused with water until wet and tightly closed for the biofumigation process for 14 days. At the end of biofumigation step, the polybags were opened for 3-5 days, then 7 day - old cucumber seedlings of var. Roberto 92 were planted and the plants were maintained in greenhouse for up to 8 weeks. Observation of the type and population of soil nematodes was carried out three times, i.e. before treatment, after treatment, and 8 weeks after planting. This experiment was designed in a completely randomized design with 6 treatments and 5 replications. The results of the study showed that the population of free-living nematodes (bacteriovorous nematodes and fungivorous nematodes) was increased in all treatment of Brassicaceae plants waste. On the other hand, there was a significant decrease in the phytonematodes population in the treatment of broccoli leaf waste. All Brassicaceae wastes was able to suppress population of Helicotylenchus sp., Rotylenchulus sp., and Xiphinema sp., significantly. Leaf waste of broccoli leaves showed the highest inhibition value against all phytonematodes genera with inhibition reached 100%
Cover Jurnal Fitopatologi Vol. 19 No. 1, January 2023
No full textThis editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 19 No. 1, January 2023This editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 19 No. 1, January 202
Komposisi Bakteri Fungsional pada Tanah Supresif dan Kondusif Layu Fusarium pada Tanaman Cabai
Get PDFComposition of Functional Bacteria on Suppressive and Conducive Soil for Fusarium Wilt on Chilli
The phenomenon of suppressive soil has been widely studied to control plant diseases in the field. Suppresive soil is characterized by its contain of functional microbes that can suppress pathogen populations. This study aims to determine and compare the composition of functional bacteria on suppressive and conducive soil of fusarium wilt. The research method consists of soil sampling in the field, isolation of functional bacteria from suppressive soil (SS) and conducive soil (CS) samples, calculation of bacterial population and type, biosafety screening, and characterization of functional bacteria in suppressing of Fusarium oxysporum. The results showed that the bacterial population in SS was higher than those in CS, especially for heat-tolerant bacteria and the fluorescent Pseudomonads. The high population of bacteria is supported by the high value of functional bacterial diversity in SS (H’ 3.70 > 3) compared to CS, which is classified as medium (1 < H’ 2.07 < 3), with the distribution of functional bacteria classified as almost evenly distributed and no dominance of certain types in SS and CS. The percentage of nonpathogenic bacteria in SS is higher than in CS, i.e. 51% and 23%, respectively. These bacteria have potential as plant growth promoting bacteria (PGPB) by producing IAA or dissolving phosphate alone of 24% and 10%, respectively in suppressive soils; and of 14% and 29%, respectively in conducive soils. Only bacteria derived from suppressive soils were able to produce IAA while dissolving phosphate with a percentage of as much as 48%.Fenomena tanah supresif telah banyak dikaji sebagai metode pengendalian penyakit tanaman alami di lapangan dan dicirikan dengan banyaknya sejumlah mikrob fungsional yang mampu menekan populasi patogen. Penelitian ini bertujuan mengetahui dan membandingkan komposisi bakteri fungsional pada tanah supresif dan kondusif layu fusarium. Metode penelitian terdiri atas pengambilan sampel tanah di lapangan, isolasi bakteri fungsional dari sampel tanah supresif (TS) dan kondusif (TK), penghitungan populasi dan jenis bakteri, penapisan berdasarkan keamanan hayati, dan karakterisasi bakteri fungsional dalam menekan Fusarium oxysporum. Hasil penelitian menunjukkan bahwa populasi bakteri pada TS lebih tinggi dibandingkan TK, khususnya bakteri toleran panas dan Pseudomonas kelompok fluorescent. Hal ini didukung dengan tingginya nilai keanekaragaman bakteri fungsional pada TS (H’ 3.70 > 3) dibandingkan TK yang tergolong sedang (1 < H’ 2.07 < 3), dengan sebaran bakteri fungsional tergolong hampir merata dan tidak ditemukan adanya dominansi jenis tertentu pada TS maupun TK. Persentase bakteri nonpatogenik pada TS lebih tinggi dibandingkan TK, masing-masing 51% dan 23%. Bakteri tersebut berpotensi sebagai plant growth promoting bacteria (PGPB) dengan memproduksi IAA atau melarutkan fosfat saja, masing-masing 24% dan 10% pada TS, sedangkan pada TK masing-masing 14% dan 29%. Hanya bakteri yang berasal dari tanah supresif yang mampu memproduksi IAA sekaligus melarutkan fosfat dengan persentase sebanyak 48%
Etiologi Penyakit Antraknosa pada Bawang Merah yang Disebabkan oleh Colletotrichum gloeosporioides
Get PDFPenyakit antraknosa dapat terjadi pada seluruh fase pertumbuhan tanaman bawang merah (Allium cepa var. aggregatum) dan belum diketahui proses perkembangannya serta karakteristik penyebabnya. Penelitian dilakukan untuk mempelajari etiologi penyakit antraknosa yang meliputi perkembangan gejala, periode inkubasi dan karakteristik patogen melalui pengujian secara in vivo dan in planta. Awal gejala ialah munculnya bercak putih yang kemudian membesar hingga menyebabkan jaringan mati. Gejala khas yang ditimbulkan ialah adanya pola konsentris berwarna jingga kecokelatan pada bercak dan memiliki periode inkubasi 2-4 hari. Karakteristik morfologi koloni patogen berwarna putih, konidium berbentuk silindris, hifa bersekat. Karakter lain ditandai dengan keberadaan aservulus dan setae. Patogen yang diidentifikasi secara molekuler menunjukkan 99.51% homologi dengan Colletotrichum gloeosporioides aksesi KT390192.1 dari China yang berasal dari tanaman tembakau.Etiology of Anthracnose Disease on Shallots Caused by Colletotrichum gloeosporioides
Anthracnose disease can occur in all growth phases of shallot plants (Allium cepa var. aggregatum); however, disease development and characteristics of the causal agent are not yet known. This research was conducted to study the etiology of anthracnose disease through in vivo and in vitro approach, which includes symptom development, incubation period, and pathogen characteristics. Early symptom of the disease is indicated by the appearance of white spots which then enlarge and finally caused the tissue dies. The typical symptom involves the presence of a concentric orange-brown pattern on the spots with incubation period of 2-4 days. The morphological characteristics of the pathogen colony are white, the conidium is cylindrical, the hyphae are septate. Other character includes the presence of acervulus and setae. Molecular identification of the pathogen showed 99.51% homology with Colletotrichum gloeosporioides accession KT390192.1 from China which originates from tobacco plants
Kejadian Pertama Infeksi Zucchini yellow mosaic virus pada Tanaman Mentimun di Padang, Sumatera Barat
Get PDFTanaman mentimun varietas lokal Padang merupakan tanaman sayuran unggulan bagi petani perkotaan karena umur panen yang pendek. Hasil survei menemukan adanya gejala bercak kuning dan klorosis dengan tulang daun hijau. Gejala ini mirip dengan infeksi Papaya ring spot virus (PRSV) yang menginfeksi mentimun di Jawa dan Zucchini yellow mosaic virus (ZYMV) yang menginfeksi mentimun pada umumnya. Deteksi virus dilakukan dengan teknik reverse transcription polymerase chain reaction (RT-PCR) menggunakan primer spesifik PRSV dan primer universal Potyvirus, perunutan dan analisis DNA. RT-PCR menggunakan primer spesifik PRSV DNA target tidak menghasilkan pita DNA, namun pita DNA dengan ukuran ±320 pb berhasil teramplifikasi dengan primer universal Potyvirus. Analisis sikuen nukleotida menunjukkan bahwa similaritas virus tertinggi sebesar 93.8% dan 93.5%, berturut-turut dengan ZYMV isolat Singapura dan India. Infeksi ZYMV merupakan kejadian pertama pada tanaman mentimun di Padang, Sumatera Barat.The First Occurrence of Zucchini yellow mosaic virus Infecting Cucumber in Padang, West Sumatra
Cucumber Local Padang variety is considered as a superior vegetable crop by urban farmers due to its early harvest time. Yellow spots and chlorosis symptoms was found on cucumber plants during field surveys conducted recently. These symptoms are similar to Papaya ring spot virus (PRSV) infection on cucumbers in Java, and common symptoms of Zucchini yellow mosaic virus (ZYMV) infection on cucurbits. Detection of the causal virus was carried out by the reverse transcription polymerase chain reaction (RT-PCR) using PRSV specific primer and universal primers of Potyvirus, DNA sequencing and analysis. DNA target was not amplified by RT-PCR using PRSV specific primer, but DNA fragment of ±320 bp was successfully amplified using Potyvirus universal primer. The nucleotide sequences analysis showed highest similarity of 93.7% and 93.5% to the ZYMV isolates from Singapore and India, respectively. This is the first report on the occurrence of ZYMV infection on cucumber plants in Padang, West Sumatra
Deteksi Penyakit Layu Fusarium pada Pisang-Pisang Lokal di Pandeglang
Get PDFPandemi layu fusarium pisang atau Panama disease, yang disebabkan oleh tropical race 4 (TR4), menjadi ancaman budi daya pisang baik industri maupun skala kecil. Di Indonesia, layu fusarium menyerang varietas ekspor pisang Cavendish dan varietas lokal populer seperti Pisang Raja, Kepok, dan Barangan. Meskipun demikian, TR4 masih belum banyak diketahui keberadaannya pada pisang-pisang lokal di berbagai wilayah Indonesia. Penelitian ini bertujuan mendeteksi TR4 pada pisang-pisang lokal Pandeglang, yang merupakan wilayah terbesar penghasil pisang di Provinsi Banten. Survey dilakukan di sembilan lokasi yang tersebar di lima kecamatan: Banjar, Cadasari, Jiput, Labuan, dan Menes. Total 13 aksesi pisang lokal menunjukkan gejala internal dan eksternal layu fusarium. Sampel pseudostem bergejala ditumbuhkan pada medium agar-agar dekstrosa kentang. Kultur spora tunggal digunakan untuk identifikasi dan isolasi DNA. Berdasarkan karakter mikroskopisnya, 27 galur Fusarium yang terdiri atas 3 komplek species berhasil diidentifikasi yaitu F. cugenangense, F. foetens dan F. oxysporum (Fusarium oxysporum species complex; FOSC), F. equiseti dan F. incarnatum (Fusarium incarnatum-equiseti species complex) dan F. solani (Fusarium solani species complex atau Neocosmospora). Deteksi molekuler duplex PCR menggunakan primer translation elongation factor-1α (Tef-1α) dan primer specific TR4, memberikan hasil positif hanya pada galur-galur yang merupakan TR4, anggota dari FOSC. Penelitian ini memperkaya pengetahuan keberadan TR4 pada daerah yang belum teridentifikasi dan potensi ancaman TR4 yang menyerang pisang-pisang lokal di Pandeglang. Analisis molekuler lebih lanjut menggunakan sikuen gen penanda atau whole genome sequencing dapat memberikan pengetahuan akan diversitas genetik Fusarium asal Pandeglang sehingga metode penanganan yang tepat dapat dilakukan.Detection of Fusarium wilt on Local Bananas in Pandeglang
Fusarium wilt of bananas or Panama disease, caused by Tropical Race4 (TR4), threatens the global banana industry. In Indonesia, the disease devastated Cavendish, an export variety, and many local bananas, such as Pisang Raja, Kepok, and Barangan. Nonetheless, TR4 incidence is unknown in many parts of Indonesia. This study aimed to detect TR4 in local bananas from Pandeglang, the most banana-produced area in Banten Province. A survey was conducted in nine Kecamatan i.e., Banjar, Cadasari, Jiput, Labuan, and Menes. In total, 13 banana accessions showed symptoms of fusarium wilt. Symptomatic pseudostem samples were plated on potato dextrose agar. The monosporic culture was derived from isolation and used for morphological identification and DNA extraction. Based on the morphological characteristics of Fusarium, 27 strains members of 3 species complexes were identified, i.e., F. cugenangense, F. foetens dan F. oxysporum (Fusarium oxysporum species complex; FOSC), F. equiseti dan F. incarnatum (Fusarium incarnatum-equiseti species complex) dan F. solani (Fusarium solani species complex, currently named as Neocosmospora). Molecular detection of TR4 was done using duplex PCR of translation elongation factor-1α (Tef-1α) and TR4 primer specific, giving positive results only on TR4 strains, members of FOSC. This study enhances our knowledge of the spreading of TR4 in an unidentified area in Indonesia. Many of the local bananas in Pandeglang is susceptible to TR4, thus threaten banana cultivation in this area. Further study on the molecular analysis using more genes will give insight into the expanding genetic diversity of Fusarium causing wilt in Banana in the Indonesian collection
Cover Jurnal Fitopatologi Vol. 19 No. 3, Mei 2023
No full textThis editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 19 No. 3, May 2023This editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 19 No. 3, May 202
Korelasi Keparahan Penyakit Layu Fusarium dengan Kelimpahan Fusarium oxysporum dan Fitonematoda: Studi Kasus Perkebunan Pisang PTPN VIII Parakansalak
Get PDFThe Severity of Fusarium Wilt Disease in Correlation to the Abundance of Fusarium oxysporum and Phytonematodes: Case Study at Banana Plantation PTPN VIII Parakansalak
Fusarium oxysporum f. sp. cubense is a soil-borne pathogen that infects banana plants and causes wilt. Several studies demonstrated that phytomatodes have a role in helping infection of the pathogens that cause wilt. This study aims to determine the relationship between phytonematodes and Fusarium oxysporum f. sp. cubense from soil and banana roots infected with fusarium wilt. Soil samples was obtained from infected banana plants and collected based on different scores of fusarium wilt disease severity. Samples were then composited based on the score, and the abundance of each pathogen is measured following samples extraction in the laboratory. The highest population of phytonematodes was 77 g-1 obtained from soil with a disease severity score of 4; and the lowest was 16 g-1 from samples with a score of 2. As for root samples, the highest population of phytonematodes (85 g-1) was obtained from plants with a score 0 and the lowest (33 g-1) was from plants with a score 3. Two species of phytonematodes were identified, i.e. Helicotylenchus sp. and Radopolus sp. Based on measurement using conventional methods showed that the abundance of Fusarium oxysporum f. sp. cubense was highest in plants with a score of 1, i.e. 8.1 × 103 cfu g-1 soil and lowest in plants with a score of 0 i.e. 1.0 × 103 cfu g-1 soil. The effect of Fusarium oxysporum f. sp. cubense to the severity of fusarium wilt shows a positive relationship of 8.3% and it may contribute to cause fusarium wilt disease by 0.6%. This pathogen can cause wilting of bananas without the help of the phytonematode species found in this study.Fusarium oxysporum f. sp. cubense ialah patogen tular tanah yang menginfeksi tanaman pisang dan menyebabkan penyakit layu. Beberapa penelitian menyatakan fitonematoda mempunyai peran dalam membantu proses infeksi patogen penyebab layu. Penelitian ini bertujuan melihat hubungan antara fitonematoda dan Fusarium oxysporum f. sp. cubense dari tanah dan perakaran pisang yang terserang layu fusarium. Tanah diperoleh dari tanaman pisang yang terinfeksi sesuai skoring keparahan penyakit layu fusarium yang berbeda. Sampel dikompositkan berdasarkan skor yang sama, lalu populasi masing-masing patogen dihitung di laboratorium. Hasil populasi absolut fitonematoda tertinggi ialah sebesar 77 ekor g-1 diperoleh pada tanah dengan skor keparahan penyakit 4; dan terkecil pada skor 2 sebesar 16 ekor g-1. Pada sampel akar tertinggi pada tanaman skor 0 sebesar 85 ekor g-1 dan terendah pada skor 3 sebesar 33 ekor g-1. Fitonematoda yang ditemukan ialah Helicotylenchus spp. dan Radopholus spp. Penghitungan Fusarium oxysporum f. sp. cubense dengan metode konvensional menunjukkan kelimpahan Fusarium oxysporum f. sp. cubense paling tinggi pada tanaman skor 1 sebesar 8.1 × 103 cfu g-1 tanah dan terendah pada tanaman dengan skor 0 sebesar 1.0 × 103 cfu g-1 tanah. Pengaruh Fusarium oxysporum f. sp. cubense terhadap keparahan penyakit layu fusarium menunjukkan hubungan positif sebesar 8.3% dan dapat menyebabkan penyakit layu sebesar 0.6%. Patogen ini dapat menyebabkan layu fusarium pada pisang tanpa bantuan spesies fitonematoda yang ditemukan dalam penelitian ini
Pengaruh Pemberian Sinar Gamma terhadap Cabai Lokal Karo Terinfeksi Penyakit Virus Daun Keriting Kuning (Begomovirus)
Get PDFCabai merupakan salah satu komoditas hortikultura bernilai ekonomi tinggi di Indonesia. Cabai lokal Karo berbatang ungu merupakan cabai yang memiliki keunggulan produksi dan pertumbuhan vegetatif tinggi, namun sangat rentan terhadap penyakit virus keriting kuning oleh begomovirus. Salah satu upaya pengendalian begomovirus ialah dengan perakitan genotipe baru yang tahan melalui induksi iradiasi sinar gamma. Penelitian bertujuan mengkaji pengaruh iradiasi sinar gamma pada benih cabai lokal Karo berbatang ungu terinfeksi begomovirus untuk mendapatkan calon genotipe mutan yang tahan. Benih dari tanaman cabai terinfeksi begomovirus diberi perlakuan iradiasi sinar gamma dengan taraf dosis 150, 200, dan 250 Gy untuk mendapatkan tanaman mutan 1 (M1). Benih sehat dan benih berasal dari tanaman sakit tanpa perlakuan digunakan sebagai tanaman kontrol. Parameter yang diamati meliputi insidensi dan keparahan penyakit, AUDPC dan deteksi begomovirus pada tanaman M1 dan benih generasi kedua (M2). Perlakuan iradiasi sinar gamma taraf dosis 150 Gy pada benih cabai terinfeksi begomovirus menunjukkan rata-rata insidensi, keparahan penyakit, dan AUDPC paling rendah dibandingkan dengan perlakuan lainnya pada tanaman mutan M1 dan M2. Berdasarkan deteksi dengan PCR pada tanaman mutan M2, dari perlakuan dosis 150 Gy didapatkan 7 calon genotipe tahan begomovirus terbanyak di antara perlakuan lainnya.The Effect of Gamma Rays on Local Chilli Infected to Pepper Yellow Leaf Curl Virus Infection (Begomovirus)Chili is a horticultural commodity that has a high economic value in Indonesia. The purple-stems Karo chili is one of local variety which has superiority in vegetative growth and production. However, it is susceptible against begomovirus infection. One of control attempt of Begomovirus is to create a new genotype resistant by using gamma ray irradiation. The research aimed to study the effect of gamma ray irradiation on begomovirus purple stems Karo local chili infecting seeds to obtain candidate of resistant genotype mutant generation 1 (M1) against begomovirus infection. The begomovirus infected seeds was irradiated by gamma rays at doses of 150, 200, 250 Gy, while untreated healthy and infected seeds were used as control. The variables observed including the disease incidence and disease severity as well as its area under disease progress curves (AUDPC) and virus detection on mutant first (M1) and mutan second generation (M2). The infected seeds irradiated by gamma ray at dose 150 Gy showed lowest disease intensity (incidence and severity) and AUDPC significantly in compare to other treatments on mutant plants first (M1) and second generation (M2). Based on the PCR detection, there are seven genotypes candidate mutants M2 which showed resistant against whitefly transmitted begomovirus