Sains Tanah - Journal of Soil Science and Agroclimatology
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Bioremediation Using Bacillus subtilis and Saccharomyces cerevisiae to Reduce Chromium in Electroplating Liquid Waste
The electroplating industry produces liquid waste containing a small number of heavy metals but is toxic. Wastewater containing chromium (Cr) absorbed into the soil will affect soil fertility. Waste management is needed so that the abiotic and biotic environment is not poisoned by Cr. Bioremediation using bacterial and fungal microbes are applicable to reduce Cr levels in electroplating liquid waste. The purpose of this research was to investigate the reduction level of Cr in electroplating liquid waste through bioremediation using Bacillus subtilis and Saccharomyces cerevisiae. Laboratory experiments were conducted using variations in microbial concentrations (102.5 cells ml-1 and 105 cells ml-1), variations in microbial types (Bacillus subtilis bacteria, Saccharomyces cerevisiae fungi, and mixtures of both microbes), and variations in incubation time (6, 12, and 24 hours). The initial Cr concentration and the results of the bioremediation process were determined by measuring the absorbance and the Cr levels using Atomic Absorption Spectrophotometry (AAS). Based on experiments, the use of Bacillus subtilis 102.5 cells ml-1 with a 24-hour incubation time reach the highest percentage reduction in Cr (88.96%), followed by 12-hours incubation time (84.73%), and 6-hours incubation time (79.21%). Furthermore, the use of a microbial mixture of Bacillus subtilis and Saccharomyces cerevisiae 102.5 cells ml-1 with 6-hours, 12-hours and 24-hours incubation time was able to reduce the levels of Cr respectively by 77.46%; 80.18% and 83.04%. Next, Saccharomyces cerevisiae 105 cells ml-1 with 6-hours, 12-hours, and 24-hours incubation time was able to reduce levels of Cr in a row by 50.17%; 52.35% and 55.63%. The results of this study indicate that the bioremediation process using the microbial Bacillus subtilis and Saccharomyces cerevisiae is proven to reduce the levels of Cr in the electroplating industry wastewater. The highest reduction results were achieved on the use of 24-hour incubation time and the use of Bacillus subtilis with a concentration of 102.5 cells ml-1 at 88.96%
Studying the Residual Effect of Zeolite and Manure on Alfisols Cation Exchange Capacity and Green Bean Yield
Nowadays the innovations in organic agriculture systems continue to be developed to achieve a sustainable agriculture system. In Indonesia with high rainfall, nutrient loss is still a major problem. The application of zeolite and manure as a soil amendment is expected to increase nutrients available in soil and cation exchange capacity of the soil in the long term. The results on the first planting season showed a significant increase in chemical soil fertility. The objective of this study is to research the effect of residue zeolite and manure on soil chemical fertility and yields of the second planting season. This study used a complete randomized block design. The factors were a dose of zeolite (Z1: 2.5 tons ha -1; Z2: 5 tons ha-1) and kind of manure (P1: quail manure; P2: cow manure), with three replications. The result showed that zeolite treatment of 5 tons ha-1 and cow manure increased the total N soil by 27.78% and 45.4% compared to the control. The treatment of quail manure increased soil organic matter 78.78% compared to the control. The treatment of cow manure increased the green bean yield 28.76% compared to the control
Effect Balance of Bokashi and Inorganic Fertilizer on Growth, Simplicia Yield, and Content Of Sinensetin of Kumis Kucing (Orthosiphon aristatus (Blume) Miq.)
Growth potential, simplicia yields, and secondary metabolites of sinensetin varieties kumis kucing can be increased through fertilization management. The aim of this research was to examine the effect balance of bokashi and inorganic fertilizer on growth, simplicia yields and secondary metabolites of sinensetin kumis kucing varieties. The design of research was a split-plot field experiment, with the main plot were varieties (Orsina 1 and Orsina 2) and subplots in bokashi fertilization of goat manure (control, bokashi 15 tons ha-1, bokashi 15 tons ha-1 + 100% inorganic fertilizer dose recommendation, bokashi 15 tons ha-1 + 50% recommended dosage of inorganic fertilizer and 100% recommended inorganic fertilizer). Inorganic fertilizer recommendations consist of Urea 100 kg ha-1, SP36 200 kg ha-1, and KCl 100 kg ha-1. The results showed that the use of bokashi 15 tons ha-1 + 50% inorganic fertilizer increased growth. Besides it also produced the highest simplicia of 48.57 g plant-1 and the highest secondary metabolite of 0.045% plant-1 in Orsina 1. Both kumis kucing varieties did not show growth differences and yields. Bokashi fertilizer can reduce the use of inorganic fertilizers in the growth and simplicia yields and potentially increase sinensetin of kumis kucing
Environmentally Sound Spatial Management Using Conservation and Land Evaluation Approach at Sloping Lands in Humid Tropic (A case study of Antang Kalang sub-district, Central Kalimantan, Indonesia)
The main problem faced by sloping lands in the humid tropic includes land degradation influencing natural ecosystem damage broadly. Land conversion and improper land-use have been widely recognized as the main cause of environmental damage since the demands for agricultural lands become greater than land resource available. The objective of this study was to determine the concept of appropriate land-use planning through environmentally sound spatial management in order to prevent land and environmental degradation. The sub-district of Antang Kalang was chosen as study area representing sloping lands in humid tropic that have a susceptibility to erosion. Conservation approach by the use of USLE erosion risk prediction model and land evaluation through land suitability classification was used in this study. The geographic information system (GIS) and remote sensing (RS) technology were applied to generate spatial basic information and to assist in spatial analysis. Two crops, upland rice, and rubber, representing food crop and estate p have been selected based on the local resource that has been existed since a long time ago. The result of spatial analysis shown that the arable land for agricultural practices covers 9,039 hectares (23.19%) while for non-arable land, it is allocated for forest preservation with total areas 29,934 hectares (76.81%). Land-use planning and land resources management involving conservation aspect and land suitability evaluation should be taken into account for farming practice at sloping lands areas since the value of soil loss potential appears as an indicator of erosion risk. Permanent cultivation system and the intercropping farming system is the option of recommended agricultural practice at sloping lands in the humid tropic that have a susceptibility to erosion
Potential Production of CH4 And N2O in Soil Profiles from Organic and Conventional Rice Fields
The horizons in soil profile will determine the magnitude of greenhouse gas production due to the difference of total organic carbon and other chemical properties. This study aimed to determine the potential production of methane (CH4) and nitrous oxide (N2O) in each horizon of soil profile from organic and conventional rice fields. Soil samples which were taken from Imogiri Bantul D.I. Yogyakarta were used to determine soil properties, the potential of CH4, and N2O productions. The correlation analysis was used to determine the relationship between the production of CH4 and N2O with soil properties. The results showed that production of CH4 and N2O will be decreased with the increase of soil depth. Production of CH4 and N2O was higher in organic rice field than in conventional rice field. The total organic carbon (TOC) correlated positively with CH4-production (r=0.89, P<0.001, n=8) and N2O-production (r=0.87, P<0.001, n=8). The nitrogen content also correlated positively with CH4-production (r=0.87, P<0.001, n=8) and N2O-production (r=0.94, P<0.001, n=8). Mitigation of CH4 and N2O emissions should consider of C and N in the soil
Characterization of Humic Substance Extracted from Andisols, Spodosols, Peat, and Lignite
Soil and other materials with high C-organic content are potential natural sources of humic substances. Until now the differences in the characteristics of such humic substances that are extracted from different sources have not been widely studied. Thus, this study was aimed to characterize humic substances extracted from andisols, spodosols, peat, and lignite. The highest C-organic (48.8%) and N (7.6%) were obtained from peat and spodosols, respectively. The andisols humic substance produced the highest total acidity (6.52 cmol kg-1). Humic substance extracted from spodosols was a mostly aliphatic group and phenolate –OH, while that of andisols and lignite contained more aromatic group and phenolate –OH, whereas the humic substance from peat was a predominantly aliphatic group and –COOH. The surface morphology of the humic substances observed using SEM showed varying densities and shapes due to differences in their respective degree of decomposition and the formation process involved. There has been significant interest in investigating the different characteristics of the humic substance. The type humic substances are important to be known considering of their positive effects on soil fertility and plant growth and development
Enhancing Chromium Phytostabilization Using Chelator (Agrobacterium sp. I26 and Manure) to Support Growth and Quality of Rice (Oryza sativa L.)
There are many of rice field which is located near the industrial area. The industrial waste contains heavy metals (chromium) which will cause contamination of rice if the waste isn’t treated properly. The used of chemical fertilizers can cause chromium contamination. It needs an effort to do remediation, such as using phytostabilization mechanism. This research aimed to determine the role of chelator in chromium phytostabilization and its influence on the growth and quality of rice. The study was conducted in Waru village, Karanganyar in May to October 2018. This research was factorial design used completely randomized block design with two factors, namely chemical fertilizers and chelator (Agrobacterium sp. I26, and manure). The parameters observed are chromium content and uptake by plant tissues (roots, shoot and rice), plant height and a number of clumps. Research output was without chemical fertilizer-chelator Agrobacterium sp. I26 that can increase the chromium uptake in roots as big as 95.38 %, increased up to 10 % in the shoot and decreased up to 92.38 % in rice compared to control. Application of Agrobacterium sp. I26 can be recommended to produce good quality and quantity of rice (good growth and free from harmful pollutants such as chromium metal)
Ratoon Systems in Tidal Lowland: Study of Groundwater Dynamics and the Change of Nutrient Status on Rice Growth
It has been widely known that crop cultivation in tidal lowland areas in the second crop (March-April) is not conducted by farmers in a maximum way. Thus, this research aims at investigating the dynamics of groundwater and its nutrient status condition for the purpose of supporting plant cultivation in the second crop after rice planting. The study was conducted in Banyu Urip Village, Tanjung Lago Telang II, Banyuasin. The plant used in this activity was paddy, with the treatment of a ratoon cultivation system. The ratoon system is paddy cultivation by using the first paddy planting season shoots. A urea treatment was given with a dose of 0 kg/ha (control) and a dose of 150 kg/ha. The results of soil analysis showed that, although not significant, there was an increase in the nutrient status of the soil condition. Therefore, it can be concluded that fertilizing was considered ineffective for the system of ratoon cultivation. The study of groundwater dynamics showed that at the early phase, a groundwater table was dropped above 30 cm (critical) but it could increase by the rain and water retention in the canal. The average of the groundwater table during the ratoon period was at the depth of 5-30 cm below the soil surface, allowing it to be suitable for plant growth. The plant growth at B treatment was better and its production was 2.8 ton/ha. This 2.8 ton/ha plant production was classified as a high category for paddy with the ratoon cultivation system. There is no effect of the fertilizer treatment on the nutrient status in the soil. Moreover, the application of fertilizer did not give a significant result on the production of rice
The Effects of Rice Husk Ash and Dolomite on Soybean Yield at Latosol Soil
The aim of this research is to evaluate the effects ofrice husk ash and dolomite application on soybean yield at latosol soil. This research was conducted at a plastic house from September to December 2016. This research employed Factorial Randomized Complete Design with two factors and three replications. The first factor is the dosage of rice husk ash, consisted of four levels (0 (control), 3.75 ton ha-1, 7.5 ton ha-1 and 15 ton ha-1), and the second factor is the dosage of dolomite consisted of three levels (0 (control), 250 and 500 kg ha-1, respectively). The results found that the application of 15 tons ha-1 rice husk significantly affected the number of full pods and total pods, but did not affect the number of the empty pods. The application of 3.75 tons ha-1 husk ash increased the seed weight and weight of 20 dry seeds. It was also found that the higher the rice husk ash dose applied, the more root nodules produced