Ilmu Pertanian (Agricultural Science)
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Drought tolerance selection of several tomato varieties by using polyethylene glycol
Full text linkA prolonged dry season can cause drought stress and have an unfavorable impact on the growth of tomato plants, resulting in crop failure. Therefore, research is needed on the resistance of tomato varieties to drought stress. The recommended technique for drought resistance screening in tomatoes is in vitro cultivation using Polyethylene Glycol (PEG). The research aimed to obtain new varieties that could be used as promising lines for varieties tolerant to drought stress. The research method used included using ingredients in the form of 5 varieties of tomatoes (Ratna, Intan, Chung, Palupi, and Amelia) and 5 concentrations of PEG chemicals (0%, 5%, 10%, 15%, and 20%). Each seed of each tomato variety that had been sterilized using hypochlorite was germinated on MS0 media, then the germinated seeds with a size of ± 0.3 cm were transferred to MS0 + PEG treatment medium with each treatment concentration. The seeds that had been planted on the PEG-treated medium were then observed for growth for ± 4 weeks. The research data were analyzed using analysis of variance (ANOVA) and followed with DMRT at the 5% significance level. Based on the results, Palupi variety is the most tolerant variety, and Ratna variety is the most intolerant variety to drought stress. The higher the concentration of PEG used, the more it inhibits plant height, root length, and fresh weight of the roots
Application of NAA and BA to Calotropis gigantea (L.) W.T. Aiton in vitro
Full text linkCrown flower (Calotropis gigantea (L.)) is a weed plant potentially to be used as raw material for textile fibers yet the utilization is not optimal. Efforts to optimize the utilization of C. gigantea are needed. This research aimed to determine the appropriate concentration of plant growth regulators, including NAA (Naphthalene Acetic Acid) and BA (Benzyl Adenine) to stimulate optimal growth of C. gigantea to be used as the basis for in vitro propagation of C. gigantea. The research was arranged in a completely randomized design (CRD). Explants were planted on Murashige and Skoog (MS) medium with various concentrations of NAA (0; 0.5; 1; 1.5; and 2 ppm) and BA (0; 1; 2; 3; and 4 ppm). Based on the research result, the addition of 3 ppm BA followed by increasing the concentration of NAA to 1.5 ppm could increase the number of C. gigantea shoots, and giving 1 ppm BA followed by adding 1.5 to 2 ppm NAA increased shoot height. The best plantlet response was found in media with 0 ppm NAA and 4 ppm BA, which could produce an optimal shoot height of 5.9 cm and a large number of shoots of 5.67 shoots. Increasing NAA concentration retarded root formation and reduced the root length and number of leaves, while the medium without NAA gave the earliest root emergence of 11 days after planting, the number of leaves of 20 strands, and the longest root of 10.9 cm. Applying a single substance BA did not accelerate all the variables observed
Effects of ameliorant application on the growth and yield of rice plants (Oryza sativa L.)
Full text linkAn ameliorant is a chemical that can be used to improve soil fertility. The improving agents include dolomite and steel slag. Steel slag is a by-product of the steel industry containing CaCO3, SiO2, CaSiO3, and MgO, which can be used to improve the growth and yield of rice plants. Dolomite, on the other hand, is a limestone rock containing MgO and CaO, which is useful in agricultural soils. This study aimed to determine the types and doses of ameliorants affecting the growth and yield of rice plants (Oryza sativa L.). The research was carried out at Sukadana Village, Ciomas Serang-Banten District. A non-factorial Group Randomized Design with seven treatments was employed in this investigation. The treatments include P0 = No treatment (control), P1 = 2 t/ha of dolomite, P2 = 2 t/ha of dolomite + 2.5 t/ha of steel slag, P3 = 1.5 t/ha of steel slag, P4 = 2.5 t/ha of steel slag, P5 = 5 t/ha of steel slag, and P6 = 7.5 t/ha of steel slag. Observation was made on steel slag characterization, plant height, the total number of tillers, the weight of 1000 grains, the amount of grain per clump, and the weight of dry-milled grain. The results showed that applying dolomite at 2 t/ha and steel slag at 2.5 t/ha could increase the weight of 1000 grains of rice, and applying steel slag at 1.5 t/ha could enhance the amount of grain per clump
The role of hydrilla (Hydrilla verticillata (L.f.) Royle) as soil protectant in improving soil physical, chemical, and biological properties
Full text linkOne of the aquatic weeds inhibiting agricultural production is hydrilla. The plants are simply removed not far from the bunds to prevent them from competing with cultivated plants. However, hydrilla can be used to improve the physical, chemical, and biological properties of the soil. As an indicator of production, chili plants were planted in the trial field in Purworejo Village, Ngantang Sub-district, Malang District from October 2020 to January 2021 to test the dose of hydrilla compost. The treatments tested consisted of hydrilla compost doses of 5 t.ha⁻¹ (P₁), 10 t.ha⁻¹ (P₂), 15 t.ha⁻¹ (P₃), and 20 t.ha⁻¹ (P₄), and NPK (16:16:16) inorganic fertilizer dose of 250 kg.ha⁻¹ (P₅) as control. The experiment was arranged in a randomized block design with three replications. Analysis of variance showed no significant difference (F0.05). Hydrilla compost contained 17.3% organic C element, while the inorganic fertilizer contained 4.27%. Hydrilla compost contained decomposing microorganisms, including Pseudomonas luorescent group, Trichoderma sp., Aspergillus sp. and Penicillium sp. The optimum dose of hydrilla compost for chili plants is 20 t.ha⁻¹
Effects of rice husk charcoal on the resistance of Jali (Coix lacryma-jobi L.) to drought stress during generative phase
Full text linkThe production of jali (Coix lacryma-jobi L.) under stress can be increased by the application of a soil enhancer, called rice husk charcoal. This study aimed to determine the effect of rice husk charcoal on the yield of jali plants under drought stress and to determine the minimum soil water content from the interval time of watering to ensure that plants can still produce grains. The treatment (interval time watering and weight of rice husk charcoal) was arranged in a split-plot design. The main plot was the interval time of watering, consisting of 3 levels: every two days, every four days, and every six days. The weight of rice husk charcoal as sub-plots consisted of 3 levels: 0 t ha⁻¹, 10 t ha⁻¹, and 20 t ha⁻¹. The data were analyzed using analysis of variance (ANOVA) and further tested using Duncan Multiple Range Test (DMRT) at a significance level of 5%. The results showed that rice husk charcoal could not increase the yield of jali plant under drought stress. Jali plants could still produce grains at a watering interval time of every six days with a minimum soil moisture content of 11.85%, or equivalent to 35.54% of field capacity
Minimum tillage on vertisols lowland increases rice production and soil properties
Full text linkCurrent land management is a challenge for wetlands vertisol soil types, especially on the rice production, which often clashes with future soil quality. This study aimed to obtain optimal performance of rice growth and physical and chemical properties of vertisol soil on various tillage systems. The study was carried out in the vertisol rice fields of Batu Bolong sub-village, Ungga village, Southwest Praya District, Central Lombok Regency, West Nusa Tenggara during the second growing season (March-October) in 2021. The experimental design was arranged in a Completely Randomized Design consisting of three levels of: no-tillage as T1, minimum tillage as T2, and maximum tillage as T3, with each treatment being repeated five times. The use of production inputs and infrastructure was uniform in all experimental plots. The results showed that T2 treatment had some of the best parameters for rice growth and yield as well as the physical and chemical properties of the land. Plant height and number of tillers at T2 were significantly more maximal in observing the vegetative phase. The dry weight of 1000 grains and rice productivity from the highest were respectively T2 > T1 > T3. The physical and chemical parameters of the soil also showed the same order. Thus, it can be said that minimum tillage is the best tillage system to obtain the growth and yield of rice in the second growing season and the most optimal physical and chemical properties of vertisol soil
Genotyping of rice (Oryza sativa L.) plants according to their root distribution pattern and their tolerance to drought
Full text linkDrought condition is one of the major problems in producing rice in Indonesia. In plant breeding, selection is the main step to create superior varieties. An indicator of drought stress-tolerant rice varieties is the root distribution pattern because it describes the behavior of the roots in searching for water for photosynthesis and metabolism processes. One of the morphological traits related to drought tolerance in rice is root growth and development. This study aimed to determine the morphological and anatomical characteristics of drought-tolerant rice roots and identify drought-tolerant genotypes. The experiments were arranged in a factorial randomized block design with four replications. The first factor was genotype, consisting of ten genotypes. The second factor was drought stress, consisting of two environments without drought and with drought stress. Drought was given only in the vegetative phase, i.e., 1–14 days after planting. WINDEX analysis was performed to determine rice drought tolerance and identify drought-tolerant genotypes. The results showed that three out of the ten tested genotypes had higher WINDEX values, namely BP30411f (7.62), B13983-KA-6-3 (7.99), and BP29790d-PWK-2-SKI-1-3 (9.25). Based on the root distribution pattern, plants with longer primary root lengths, more seminal roots, longer seminal root lengths, and high root angles were predicted to be drought tolerant characteristics. Selection of these characters could be used in future rice breeding programs to obtain plants with superior genotypes
Effects of mycorrhiza and phosphate fertilizers on the growth and yield of foxtail millet (Setaria italica L.) under drought stress conditions
Full text linkMillet is a cereal plant that’s potential for rice substitution. This plant is adaptive to be cultivated in dry land but has a tolerance limit to drought stress. Mycorrhiza and phospate (P) fertilizer treatments help plants adapt to this condition. This study aimed to determine the effects of phosphate fertilizer and mycorrhiza as well as their interaction effects on the growth and yield of foxtail millet (Setaria italica L.) under drought stress. The experiment was carried out from January to June 2020 in the experimental farm, Faculty of Agriculture, University of Jenderal Soedirman. The research was arranged in a factorial randomized complete block design consisting of two factors. The first factor was the dose of SP-36 fertilizer per polybag, namely P0 = 0 g, P1 = 37,5 kg.ha⁻¹ (25%), P2 = 75 kg.ha⁻¹ = 0.88 g/polybag (50%), and P3 = 150 kg.ha⁻¹. The second factor was the dose of mycorrhiza biofertilizer, namely M0 = 0 g.polibag⁻¹, M1 = 33.3 g. polibag⁻¹ and M2 = 66.6 g.polybag⁻¹. The treatment was replicated three times. The data observed were analyzed using the F test, continued with DMRT test at p=0.05. The results showed that SP-36 fertilizer application at half of the recommended dose (0.88 g/polybag) could increase growth variables, such as leaf area, panicle length, and seed weight. Mycorrhizae application 33.3 g/polybag could improve variables such as plant height, leaf area, panicle length, and seed weight. It also accelerated the initiation of panicle emergence compared to control
The role of mycorrhiza and humic acid on quantitative and qualitative traits of faba bean plant under different fertilizer regimes
Full text linkIn sustainable agricultural ecosystem management, a key focus is on minimizing chemical fertilizer use and maximizing the use of non-chemical alternatives. Our study was designed to explore the impact of mycorrhizal fungi and humic acid (HA) on the quantitative and qualitative characteristics of faba beans under varying chemical fertilizer levels. The experiment involved different treatments, which included HA and mycorrhiza in four variations (control, HA, HA+ mycorrhiza, and mycorrhiza alone), and nitrogen, phosphorus, and potassium (NPK) fertilizers at three different dosages (50%, 75%, and 100% of the recommended amount). The findings revealed that combining HA with mycorrhiza significantly influenced root colonization, with the highest chlorophyll a concentration (1.58 mg g⁻¹) observed in the HA+ mycorrhiza treatment at 75% NPK. This treatment also led to the highest counts in seeds per pod, number of pods, and weight of 100 seeds. Between the 100% and 75% NPK levels in the HA+ mycorrhiza treatment, no significant differences were noted in terms of grain and biological yield. The greatest grain yields were measured at 4356 kg ha⁻¹ and 4322 kg ha⁻¹ for the HA+ mycorrhiza treatment at 100% and 75% NPK, respectively. Additionally, the highest concentrations of Fe, N, P, K, and Zn were observed with the HA+ mycorrhiza application at the 100% NPK level
