Ilmu Pertanian (Agricultural Science)
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Pengaruh Strain Agrobacterium Terhadap Efesiensi Transformasi Genetik Jagung Genotipe Hibrida Hill
No full textThe development of smart screening technology kit (SST-Kit) for sorting cassava cultivars based on cyanide analysis
Get PDFCyanides are deadly toxic substances commonly found in cassava root tuber. Higher content of cyanides causes bitter taste of the root tuber. Therefore, analysis of cyanide content has been used as one of criteria for bitterness in cassava. The substitute common method used for this purpose was organoleptic test that could lead to poisoning, or argentometry, which is complicated, time-consuming, and requires lots of reagents. Therefore, a fast, easy, and safe method is strongly demanded. In this work, a smart screening technology kit (SST-Kit) was developed using ninhydrin reagent. This simple, fast, and low-cost method can be applied in the field for the analysis of cyanide levels in cassava. This method was optimized to the concentration of sodium carbonate from 0.5 to 2.0% by measuring the red color intensity produced under various times of reading from 1 to 3 minutes and three sample preparation techniques. The optimum condition for SST-Kit to read the cyanides level was achieved under the condition of 0.5% of sodium carbonate with a time readout of 2 minutes, using grinding preparation technique followed by dilution. The SST-kit had been applied on cassava samples with the accuracy of more than 90%
Effect of saline irrigation method on the growth of rice (Oryza sativa L.)
Get PDFA field experiment was carried out to study rice growth under salinity stress in permanently flooded, intermittent, and saturated soil conditions. The experiment was conducted at field station of Faculty of Agriculture, Universitas Gadjah Mada located in Bantengan village, Banguntapan, Bantul, DIY, from August to November 2015. The treatments were arranged in a split-plot randomized complete block design, consisting of three irrigation methods as the main plot and two levels of salinity as the subplot, thereby resulting in six treatment combinations with three replications within each combination treatment. The three irrigation methods were permanent irrigation (flooded soil), intermittent irrigation (wet/dry soil), and irrigation above field capacity (saturated soil). The two levels of salinity were fresh water as control treatment (EC 0.3 dS.m-1) and saline water (EC 5 dS.m-1). The results of the experiment showed that there was no significant interaction effect of irrigation method and salinity on all observed rice growth variables. Salt stress negatively affected the rice growth, including plant height, plant dry weight, leaf area, and leaf area ratio (LAR). Irrigation method significantly affected plant dry weight and number of tillers at 3 and 9 weeks after planting. Permanent irrigation resulted in the highest plant growth compared to intermittent irrigation and above field capacity condition
Effects of water flow rate and surface cover plant density on the growth of duckweed (Lemna minor L.)
Get PDFGlobally, agriculture sector is facing unprecedented challenges in producing fertilizers and increasing the amount of fertilizer production without having negative impact on the environment. Thus, the organic fertilizers are needed to be produced as they do not give any damages to the environment. Duckweed plant has a lot of potentials that can be used in the agriculture sector. This plant can breed in approximately 16-48 hours by splitting. The water needs and its breeding speed ability are the basis for conducting this research. The research objective was to determine the effect of water flow rate and surface cover plant density on the growth and yield of duckweed plants. This research was conducted in November–December 2018 in Cangkringan District, Sleman, Special Region of Yogyakarta, Indonesia. The experiment was arranged in a split plot design. The main plot was irrigation water flow rate, consisting of two levels, namely large water flow rate (0.336 L.second-1) and small water flow rate (0.085 L.second-1). The subplot was the density of the duckweed plant surface cover, consisting of 10%, 20%, 40% and 60%. The results of this study indicated there was no effect of water flow rate on the plant growth, yield, and yield quality of duckweed plants. The C/N ratio of the duckweed plants fulfilled the requirement to be used as green manure
