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Data related to article titled "A quantitative assay for Ca2+ uptake through normal and pathological hemichannels".
No full textHemichannels (HCs) are large pore hexameric structures composed of connexin (Cx) subunits that allow the exchange of ions, metabolites, and a variety of other molecules between cell cytoplasm and extracellular milieu. HC inhibitors are attracting growing interest as drug candidates because deregulated fluxes through HCs have been implicated in a plethora of genetic conditions and other diseases. HC activity has been mainly investigated by electrophysiological methods and/or using HC-permeable dye uptake measurements. Here, we present an all-optical assay based on fluo-rometric measurements of Ca2+ uptake with a Ca2+-selective genetically encoded indicator (GCaMP6s) that permits optical tracking of cytosolic Ca2+ concentration ([Ca2+]cyt) changes with high sensitivity. We exemplify use of the assay in stable pools of HaCaT cells overexpressing human Cx26, Cx46, or the pathological mutant Cx26G45E under control of a tetracycline (Tet) responsive element (TRE) promoter (Tet-on). We demonstrate its usefulness for the characteriza-tion of new monoclonal antibodies (mAb) targeting the extracellular domain of the HCs. Although we developed the assay on a spinning disk confocal fluorescence microscope, the same method-ology can be extended seamlessly to high-throughput high-content platforms to screen other kinds of inhibitors and/or to probe HCs expressed in primary cells and microtissues
Application of Patients Reported Outcome measures in Cochlear Implant patients: implications for the design of specific rehabilitation programs
Get PDFIntroduction: Cochlear implants (CI) had been developed to enable a satisfying verbal communication, music experiences have remained in the background of research and development and consequently many CI users are dissatisfied by the music they listen to.
Nonetheless, concise indications for clinicians to test music abilities and prescribe rehabilitation programs are still lacking. The main aim of the present study was to test the utility of the application of two different Patient Reporting Outcomes (PRO) measures in a group of CI users. A secondary objective was to identify items capable of driving the indication and design specific
music rehabilitation programs for CI patients.
Material and Methods: A consecutive series of 73 CI patients referred to the Audiology Unit - University of Padova - was enrolled from November 2021 to May 2022 and evaluated with audiological battery test and PRO measures: Musica e Qualità della Vita (MUSQUAV) and Nijmegen Cochlear Implant Questionnaire (NCIQ) Italian version.
Results: The Reliability analysis showed good consistency between the different PRO measures, (Cronbach alpha = 0.873). After accounting for epidemiological and clinical variables, PRO measures showed a correlation with audiological outcomes in only one case (rho=-0.170 for NCIQ-T with CI-Pure Tone Average. A willingness for musical rehabilitation was present in 63% of
patients, (Rehab Factor, mean value of 0.791±0.675).
Conclusions: we support the role of the application of MUSQUAV and NCIQ to improve the clinical and audiological evaluation of CI patients. Moreover, we proposed a derivative item, called Rehab Factor, which could be used in clinical practice and future studies to clarify the indication and priority of specific music rehabilitation programs
Loading Experiment Database - Venice Lagoon (Italy)
No full textThis database collects the data from two original loading experiments carried out in the Venice Lagoon (Italy) at the sites of Lazzaretto Nuovo and La Grisa
Data related to eBiomedicine 2023 Article Number 104453 titled "Antibody gene transfer treatment drastically improves epidermal pathology in a keratitis ichthyosis deafness syndrome model using male mice"
No full textSUMMARY
Background
Keratitis ichthyosis deafness (KID) syndrome is a rare disorder caused by hemichannel (HC) activating gain-of-function mutations in the GJB2 gene encoding connexin (Cx) 26, for which there is no cure, or current treatments based upon the mechanism of disease causation. The recent development of specific monoclonal antibodies (mAbs) that block the acquired HC activity, and the availability of mouse models that replicate the skin pathology of KID syndrome, allow this deficiency to be directly addressed. In this study, we aimed to assess inhibition of mutant Cx HC activity with anti-HC monoclonal antibodies as therapeutic target in KID syndrome.
Methods
We used Adeno Associated Virus (AAV) mediated mAb gene transfer (AAVmAb) to treat the epidermal features of KID syndrome in a mouse model of the human disease with a well-characterized HC blocking antibody.
Findings
We demonstrate that in vivo AAVmAb treatment, via caudal vein injection of the mAb-encoding AAV vector, significantly reduced the size and thickness of KID lesions, in addition to blocking activity of mutant HCs in the epidermis in vivo. We also show that AAVmAb treatment eliminated abnormal keratinocyte proliferation and enlarged cell size, decreased apoptosis, and restored the normal distribution of keratin expression.
Interpretation
Our findings reinforce the critical role played by increased HC activity in the skin pathology associated with KID syndrome. They also underscore the clinical potential of anti-HC mAbs coupled with genetic based delivery systems for treating the underlying mechanistic basis of this disorder. Inhibition of HC activity is an ideal therapeutic target in KID syndrome, and the genetic delivery of mAbs targeted against mutant HCs could form the basis of new therapeutic interventions to treat this incurable disease.
Funding
Fondazione Telethon grant GGP19148 to FM; University of Padova grant Prot. BIRD187130 to FM; Foundation for Ichthyosis and Related Skin Types (FIRST) to TWW; National Institutes of Health grant EY 026911 to TWW
EJSP Corpus 1971-2016
No full textDataset used in the study related to the following paper: Rizzoli, V., Castro, P., Tuzzi, A., & Contarello, A. (2019). Probing the history of social psychology, exploring diversity and views of the social: Publication trends in the European Journal of Social Psychology from 1971 to 2016. European Journal of Social Psychology, 49(4), 671-687. https://doi.org/10.1002/ejsp.252
Data related to article titled "A quantitative assay for Ca2+ uptake through normal and pathological hemichannels"
No full textHemichannels (HCs) are large pore hexameric structures composed of connexin (Cx) subunits that allow the exchange of ions, metabolites, and a variety of other molecules between cell cytoplasm and extracellular milieu. HC inhibitors are attracting growing interest as drug candidates because deregulated fluxes through HCs have been implicated in a plethora of genetic conditions and other diseases. HC activity has been mainly investigated by electrophysiological methods and/or using HC-permeable dye uptake measurements. Here, we present an all-optical assay based on fluo-rometric measurements of Ca2+ uptake with a Ca2+-selective genetically encoded indicator (GCaMP6s) that permits optical tracking of cytosolic Ca2+ concentration ([Ca2+]cyt) changes with high sensitivity. We exemplify use of the assay in stable pools of HaCaT cells overexpressing human Cx26, Cx46, or the pathological mutant Cx26G45E under control of a tetracycline (Tet) responsive element (TRE) promoter (Tet-on). We demonstrate its usefulness for the characteriza-tion of new monoclonal antibodies (mAb) targeting the extracellular domain of the HCs. Although we developed the assay on a spinning disk confocal fluorescence microscope, the same method-ology can be extended seamlessly to high-throughput high-content platforms to screen other kinds of inhibitors and/or to probe HCs expressed in primary cells and microtissues
Diagrammi stratigrafici - Scavo archeologico Fondi Cossar Aquileia 2009-2015 - Unipd
No full textIl dataset raccoglie tutti i diagrammi stratigrafici (Harris Matrix) relativi allo scavo condotto presso i fondi Cossar di Aquileia dall'Università di Padova, la Soprintendenza del Friuli Venezia-Giulia e la Fondazione Aquilei
Urinary metabotypes of newborns with perinatal asphyxia undergoing therapeutic hypothermia
No full textStudy’s minimal underlying data set (Article title: Urinary metabotypes of newborns with perinatal asphyxia undergoing therapeutic hypothermia
Modeling-based design specifications for microfluidic gradients generators for biomedical applications
No full textThis work proposes a comprehensive approach to optimize the design of microfluidic concentration gradient generators (MGGs) for biomedical applications
Blue Carbon assessment of the salt marshes of the Venice Lagoon, Italy
No full textDataset for the Blue Carbon assessment of the salt marshes of the Venice Lagoon, Ital