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Bruton’s tyrosine kinase signaling in health and disease: a molecular perspective
Get PDFIn this thesis, we studied the role of Bruton's tyrosine kinase (Btk) in B cells. We specifically investigated the role of different receptor signaling pathways in which Btk is involved and the contribution of Btk signaling to the development of leukemia and autoimmune disease
Glucose Metabolism and Liver Fat in Early Life
Get PDFThe first objective of this thesis was to examine the hypothesis that maternal early-pregnancy glucose concentrations are associated with adverse fetal, pregnancy, birth and child cardio-metabolic outcomes. Also, as second objective, we studied potential determinants of a specific outcome, childhood liver fat, as well as the cardio-metabolic consequences of childhood liver fat
Macromolecular Interactions in Cartilage Extracellular Matrix Vary According to the Cartilage Type and Location
No full textObjective: To investigate GAG-ECM (glycosaminoglycan–extracellular matrix) interactions in different cartilage types. To achieve this, we first aimed to determine protocols for consistent calculation of GAG content between cartilage types. Design: Auricular cartilage containing both collagen and elastin was used to determine the effect of lyophilization on GAG depletion activity. Bovine articular, auricular, meniscal, and nasal cartilage plugs were treated using different reagents to selectively remove GAGs. Sulfated glycosaminoglycan (sGAG) remaining in the sample after treatment were measured, and sGAG loss was compared between cartilage types. Results: The results indicate that dry weight of cartilage should be measured prior to cartilage treatment in order to provide a more accurate reference for normalization. Articular, meniscal, and nasal cartilage lost significant amounts of sGAG for all reagents used. However, only hyaluronidase was able to remove significant amount of sGAG from auricular cartilage. Furthermore, hyaluronidase was able to remove over 99% of sGAG from all cartilage types except auricular cartilage where it only removed around 76% of sGAG. The results indicate GAG-specific ECM binding for different cartilage types and locations. Conclusions: In conclusion, lyophilization can be performed to determine native dry weight for normalization without affecting the degree of GAG treatment. To our knowledge, this is the first study to compare GAG-ECM interactions of different cartilage types using different GAG extraction methods. Degree of GAG depletion not only varied with cartilage type but also the same type from different anatomic locations. This suggests specific structure-function roles for GAG populations found in the tissues
Extractivism, Rural Livelihoods and Accumulation in a “Climate-Smart” World: The rise of green extractivism
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Interorganizational Governance in Projects: Contracts and collaboration as alignment mechanisms
Get PDFThis dissertation studies various aspects of interorganizational governance in projects, focusing on how contracts and collaboration can effectively align diverging interests of multiple parties
Building Bridges: Policy Transfer and Translation of the Dutch Delta Approach in South and Southeast Asia
Get PDFThis dissertation explores explanations why policy transfer is (in)effective. This is done by means of a literature review and case studies in which Dutch delta management is transferred to Bangladesh, Vietnam and Jakarta (Indonesia)
Medication for Osteoarthritis Pain : Use and evidence for opioids and neuropathic pain medication
Get PDFClinical and Immunological Changes After Laparoscopic Roux-en-Y Gastric Bypass for Morbid Obesity
Get PDFClinical and immunological changes after a laparoscopic Roux-en-Y gastric bypass for morbid obesity are described in this thesis. Additionally, long term complications after bariatric surgery were assessed