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Ibuprofen removal from aqueos solution by sorption on different low-cost sorbents : diploma thesis
No full textPovećana i nekontrolirana uporaba farmaceutika diljem svijeta postaje sve značajniji ekološki problem. Ibuprofen je među najprodavanijim lijekovima bez recepta u svijetu, što dovodi do porasta njegove koncentracije u postrojenjima za obradu otpadnih voda, a uslijed nedovoljne učinkovitosti konvencionalnih metoda obrade, pojavljuje se i u vodnim tijelima koja se crpe za vodoopskrbu. Zbog brojnih fizioloških, hormonalnih i reproduktivnih poremećaja koje uzrokuje kod vodenih organizama, jasna je potreba za razvijanjem metoda učinkovitijeg uklanjanja ibuprofena iz voda. U ovome radu ispitano je uklanjanje ibuprofena iz vodene otopine koncentracije ≈ 1 mg/L sorpcijom na prirodnom zeolitu klinoptilolitu, njegovim modificiranim oblicima (natrijevom, željezovom i sumporovom) te na nusproizvodima prerade voća - košticama maslina, trešanja i višanja. Samo su koštice višanja pri ispitanim eksperimentalnim uvjetima pokazale učinkovitost od 30,15 % u uklanjanju ibuprofena iz vodene otopine s postignutim kapacitetom od 0,025 mg/g. Neučinkovitost ostalih sorbenasa je najvjerojatnije rezultat elektrostatskog odbijanja njihove izrazito negativne površine i deprotonirane molekule ibuprofena pri pH vrijednostima tijekom sorpcije. Buduća istraživanja trebalo bi usmjeriti ka modifikaciji materijala s ciljem poboljšanja njihovih sorpcijskih svojstava, ali i prilagođavanju eksperimentalnih uvjeta svojstvima onečišćujuće tvari, u svrhu postizanja što potpunijeg uklanjanja. Također, treba raditi na uvrštavanju farmaceutika na listu prioritetnih tvari u analizi vode budući da još uvijek ne postoji zakonska regulativa s maksimalno dopuštenim koncentracijama farmaceutika u prirodnim vodama koje se crpe za vodoopskrbu. U konačnici, nužno je poraditi na odgovornom zbrinjavanju lijekova kako bi se spriječili dugoročni učinci na bioraznolikost vodenih organizama, ali i ljude koji ovise o tim ekosustavima.The increased and uncontrolled use of pharmaceuticals worldwide is becoming a significant environmental problem. Ibuprofen is among the most sold over-the-counter drugs in the world, which leads to an increase in its concentration in wastewater treatment plants. Due to the insufficient efficiency of conventional water treatment methods, it also appears in water bodies that are pumped for water supply. Due to the numerous physiological, hormonal and reproductive disorders it causes in aquatic organisms, there is a clear need to develop methods for more effective removal of ibuprofen from water. In this paper, the removal of ibuprofen from an aqueous solution with a concentration of ≈ 1 mg/L by sorption on natural zeolite clinoptilolite, its modified forms (sodium, iron and sulfur) and on fruit processing by-products - olive, cherry and sour cherry pits was examined. Only sour cherry pits showed effectiveness (30.15%) in ibuprofen removal from aqueous solution under the tested experimental conditions, with a capacity of 0.025 mg/g. The ineffectiveness of other sorbents is most likely the result of electrostatic repulsion of their extremely negative surface and deprotonated ibuprofen molecule at pH values during sorption. Future research should be focused towards materials modification with the aim of improving their sorption properties, but also to the adaptation of experimental conditions to the properties of the pollutant, in order to achieve more effective treatment. Efforts should be made to include pharmaceutical residues on the list of priority substances in water analysis, as there is still no legal regulation specifying the maximum allowable concentrations of pharmaceuticals in natural waters used for water supply. Ultimately, responsible disposal of pharmaceutical products is necessary to prevent long-term effects on the biodiversity of aquatic organisms, as well as the people who depend on these ecosystems
Influence of storage conditions on the quality of virgin olive oils : diploma thesis
No full textDjevičansko maslinovo ulje najviše razine kvalitete je iznimno vrijedan proizvod ploda masline zbog brojnih zdravstvenih koristi kao i poželjnih senzorskih svojstava pa mu je cijena znatno viša u odnosu na cijenu drugih biljnih ulja. Kako bi se izbjegle potencijalne mogućnosti patvorenja drugim uljima ova ulja su predmet stalnih provjera kvalitete i autentičnosti. Kvaliteta djevičanskih maslinovih ulja ovisi o mnogo čimbenika, a jedan od važnijih je način i uvjeti skladištenja. Da bi se usporile promjene u fizikalno-kemijskom sastavu i senzorskim svojstvima ulja važan je ispravan način čuvanja (skladištenja). U prvom redu ulje treba čuvati od negativnih utjecaja svjetla, temperature, kisika i vlage. Cilj ovog diplomskog rada je utvrditi kako različiti uvjeti čuvanja ulja utječu na kvalitetu djevičanskih maslinovih ulja. Analize su provedene na uljima iz maslinika sa dva lokaliteta. Ulja sorti Oblica i Leccino iz jednog maslinika te ulja sorti Oblica i Levantinka iz drugog maslinika. Ulja su čuvana na dva načina; u tamnoj staklenoj boci, na tamnom mjestu pri sobnoj temperaturi te u inox spremniku s natprostorom dušika odnosno bez natprostora dušika. Ispitivanja kvalitete provodila su se tromjesečno kroz period od 18 mjeseci. Rezultati analiza pokazali su kako protekom vremena skladištenja rastu vrijednosti fizikalno-kemijskih pokazatelja kvalitete, smanjuju se intenziteti pozitivnih osjetilnih svojstava te opada sadržaj ukupnih fenolnih spojeva u uljima.Virgin olive oil of the highest level of quality is an extremely valuable product of olive fruit due to numerous health benefits as well as desirable sensory properties, so its price is significantly higher than the price of other vegetable oils. In order to avoid the potential adulterations with other seed oils, these oils are the subject of constant quality and authentication check. The quality of the virgin olive oils depends on many factors, and one of the most important is storage conditions. In order to slow down changes in the physical- chemical composition and sensory properties of oil, the propriate way of storage is important. In the first place, the oil should be kept from the negative effects of light, temperature, oxygen and humidity. The aim of this diploma thesis is to determine how different conditions of storage affect the quality of virgin olive oils. The analyses were carried out on oils from olive orchards from the two localities. Oils from the Oblica and Leccino variety from same olive orchard and oils from Oblica and Levantinka variety from other olive orchard. The oils were storaged in two ways; in a dark glass bottle in dark place at room temperature and in the stainless steel tank with nitrogen or no nitrogen above. Quality analyzes were conducted every 3 months over a period of 18 months. The results of the analyses have shown that as the storage time progresses, the values of physico-chemical quality indicators increase, the intensities of positive sensory properties decrease and the content of total phenolic compounds in oils decreases
Development and evaluation of the method from the determination of tiopronin in pharmaceutical preparations using the sequential injection technique with a spectrophotometric detector : diploma thesis
No full textPrimjenom slijedne analize injektiranjem uz spektrofotometrijski detektor razvijena je i vrednovana nova kinetička metoda za određivanje tiopronina (N-2-merkaptopropionil-glicin, MPG) u farmaceutskim pripravcima. Metoda se temelji na redoks-reakciji u kojoj tiopronin reducira žuti bis(batokuproindisulfonato)kuprat(II) kompleks u stabilan bis(batokuproindisulfonato)kuprat(I) kompleks, karakteristične žuto-narančaste boje, s apsorpcijskim maksimumom pri valnoj duljini od 483 nm. Apsorbancija formiranog kompleksa kontinuirano se mjerila pomoću UV-Vis spektrofotometra, pri čemu su se mjerenja izvodila u triplikatu. Univarijantnom metodom optimizirani su kemijski i protočni parametri sustava, na osnovu čega je konstruirana krivulja umjeravanja za određivanje linearnog dinamičkog područja metode. Regresijskom analizom dobivena je jednadžba pravca y = 5897x + 0,0176, s linearnim rasponom koncentracija analita od 8,0 × 10 -7 do 6,0 × 10 -5 mol L -1 . Određene su granica dokazivanja od 2,54 × 10 -7 mol L -1 i granica određivanja od 8,48 × 10 -7 mol L -1 . Metoda je vrednovana u pogledu točnosti, iskoristivosti i preciznosti, uključujući ponovljivost, učestalost i doseg reakcije. Točnost metode potvrđena je iskoristivošću između 100,70 i 110,81 %. Ponovljivost je bila zadovoljavajuća, uz relativno standardno odstupanje od 4,66 %. U jednom satu moguće je provesti 12 analiza u triplikatu, što daje ukupno 36 analiza. Reakcijski doseg je 90,50 %. Osim acetilsalicilne kiseline i visokih koncentracija borne kiseline, niti jedna od uobičajenih pomoćnih tvari u farmaceutskim proizvodima ne interferira. Razvijena metoda uspješno je primijenjena za određivanje tiopronina u farmaceutskom pripravku Captimer ® , a dobiveni rezultati pokazali su visoku korelaciju sa standardnom farmakopejskom metodom.Using sequential injection analysis with a spectrophotometric detector, a new kinetic method for the determination of tiopronin (N-2-mercaptopropionyl-glycine, MPG) in pharmaceutical preparations was developed and validated. The method is based on a redox reaction in which tiopronin reduces the yellow bis(bathocuproinedisulfonate)cuprate(II) to a stable bis(bathocuproinedisulfonate)cuprate(I), characterized by a yellow-orange color, with an absorption maximum at a wavelength of 483 nm. The absorbance of the formed complex was continuously measured using a UV-Vis spectrophotometer, with measurements performed in triplicate. The chemical and flow parameters of the system were optimized using the univariate method, based on which a calibration curve was constructed to determine the linear dynamic range of the method. Regression analysis yielded the equation y = 5897x + 0.0176, with a linear range of analyte concentrations from 8.0 × 10 -7 to 6.0 × 10 -5 mol L -1 . The limits of detection and quantification were determined as 2.54 × 10 -7 mol L -1 and 8.48 × 10 -7 mol L -1 , respectively. The method was evaluated in terms of accuracy, efficiency, and precision, including reproducibility, analysis frequency and reaction yield. The accuracy of the method was confirmed with efficiency ranging from 100.70% to 110.81%. The reproducibility was satisfactory, with a relative standard deviation of 4.66%. In one hour, 12 triplicate analyses can be performed, resulting in a total of 36 analyses. The reaction yield was 90.50%. Apart from acetylsalicylic acid and high concentrations of boric acid, none of the commonly present excipients in pharmaceutical products interfered with the method. The developed method was successfully applied for the determination of tiopronin in the pharmaceutical preparation Captimer®, and the obtained results showed a high correlation with the standard Pharmacopoeial method
ICP-MS analysis of mineral elements in wines from Konavle, Pelješac, Hvar and Korčula : graduate thesis
No full textU ovom radu provedena je analiza 30 mineralnih elemenata u 107 uzoraka različitih sorti crnog, bijelog i rosé vina s područja Konavla, Pelješca, Hvara i Korčule. Analiza je provedena korištenjem analitičke tehnike masene spektrometrije povezane s induktivno spregnutom plazmom (ICP – MS). Prije same analize izmjerena je pH vrijednost uzoraka. Svaki uzorak je prije ICP - MS analize podvrgnut mirovalnoj digestiji u zatvorenim teflonskim posudama uz korištenje HNO 3 i H 2 O 2 . Od 30 analiziranih elemenata, berilij, kobalt, živa i vanadij nisu detektirani ni u jednom od 107 uzorka, a rezultati za olovo nisu razmatrani. Šest uzoraka pokazalo je koncentraciju bakra iznad dopuštene (1000 μg/L), uz istovremeno povišene, ali dopuštene vrijednosti nikla, kadmija, kositra, željeza i antimona, što sugerira mogući zajednički izvor kontaminacije. Cink je pokazao poveznicu sa željezom i bakrom. Svi ostali elementi su unutar dopuštenih granica, ali esencijalni su većinom prisutni u nižim koncentracijama nego u drugim istraživanjima, vjerojatno zbog tla bogatog CaCO₃ koje smanjuje dostupnost minerala zbog visokog pH.This study analyzed 30 mineral elements in 107 samples of various red, white, and rosé wines from the regions of Konavle, Pelješac, Hvar, and Korčula. The analysis was conducted using inductively coupled plasma mass spectrometry (ICP-MS). Prior to the analysis, the pH value of each sample was measured. Each sample underwent microwave digestion in closed Teflon vessels using HNO₃ and H₂O₂ before ICP-MS analysis. Out of the 30 analyzed elements, beryllium, cobalt, mercury, and vanadium were not detected in any of the 107 samples, and lead results were not considered. Six samples showed copper concentrations above the permitted limit (1000 μg/L), along with elevated but still acceptable levels of nickel, cadmium, tin, iron, and antimony, indicating a possible common source of contamination. Zinc showed a correlation with iron and copper. All other elements were within acceptable limits, but essential elements were mostly found in lower concentrations compared to other studies, likely due to CaCO₃-rich soils in Central and Southern Dalmatia, which increase soil pH and reduce mineral availability
Traffic Analysis of State Road DC60, County Road ŽC6157 and Local Road LC67171 in the Area of Glavina Donja : Master's Thesis
No full textU ovom diplomskom radu izrađena je prometna analiza odvijanja prometnih tokova za postojeće stanje raskrižja na području Glavina Donja te za planirano stanje prema kojem se predviđa rekonstrukcija u raskrižje oblika rotora. Za provođenje analize izvršeno je brojanje prometa tj. prikupljeni su podaci o veličini i strukturi prometa za jutarnji i poslijepodnevni vršni period te određena mogućnost povećanja prometa do dostizanja kapaciteta raskrižja. Analiza je provedena korištenjem modela SIDRA i primjenom HCM metodologije.In this graduation thesis, a traffic analysis of the development of traffic flows was made for the existing state of the intersection in the area of Glavina Donja and for the planned state according to which the reconstruction into a rotor-shaped intersection is foreseen. To carry out the analysis, a traffic count was carried out, i.e. the data about the volume and structure of traffic in morning and afternoon peek period was collected, and the possibility of increasing traffic until reaching the intersection's maximum capacity was determined. The analysis was conducted using the SIDRA model and applying the HCM methodology
Optimization of protein extraction methods for LC-MS/MS analysis: Preliminary analysis of the proteome of the Croatian oak plant (Quercus virgiliana (Ten.) Ten. )
No full textU ovome radu proveli smo analizu metoda ekstrakcije proteina za opisivanje proteoma lista i tkiva modificiranog hrastovom osicom biljke hrvatskog hrasta (drmun, Quercus virgiliana (Ten.) Ten.) pomoću LC-MS spektrometrije masa. Testirana su četiri različita protokola kako bi se odredila najučinkovitija metoda za ekstrakciju i izolaciju proteoma: SDC-FASP, ekstrakcija fenolom u kombinaciji sa SDC-FASP, taloženje TCA/acetonom praćeno ekstrakcijom fenolom i SDC-FASP, te taloženje TCA/acetonom u kombinaciji sa SDC-FASP. Cilj je bio utvrditi najučinkovitiju metodu za izolaciju i pročišćavanje proteina za pripremu uzoraka uz pomoć filtera (engl. filter-assisted sample preparation, FASP). Identifikacija proteina i posttranslacijske modifikacije (PTM) analizirane su korištenjem softvera ProteinPilot™ prema bazi podataka referentnog proteoma Quercus lobata. Iako su izmjereni niski prinosi proteina za sve protokole, taloženje TCA/acetonom praćeno ekstrakcijom fenolom i SDC-FASP dalo je najveći broj identificiranih proteina. Ukupni ionski kromatogrami (engl. total ion chromatograms, TIC) također su potvrdili niske signale peptidnih iona koji ukazuju na niske koncentracije proteina. Usporedna analiza uzoraka lista i tkiva modificiranog hrastovom osicom (engl. gall-wasp) pokazala je različite proteomske profile. Tkiva lista bila su bogata proteinima povezanim s fotosintezom, energetskim metabolizmom i odgovorima na stres, dok tkiva modificiranog hrastovom osicom sadrže proteine povezane s prikupljanjem hranjivih tvari, regulacijom razvoja i imunološkom modulacijom, odražavajući fiziološke učinke modificiranog tkiva na domaćina.In this study, we performed an analysis of protein extraction methods suitable for proteomic experiments of oak leaves and oak gall wasp-modified tissue from Quercus virgiliana using LC-MS-based mass spectrometry. Four extraction protocols were tested for optimal protein isolation: SDC-FASP, phenol extraction combined with SDC-FASP, TCA/acetone precipitation with phenol extraction and SDC-FASP, and a combination of TCA/acetone precipitation and SDC-FASP. The aim was to determine the most effective method for protein isolation and purification for filter-assisted sample preparation (FASP). Protein identification and post-translational modifications (PTMs) were analyzed using ProteinPilot™ with reference to the Quercus lobata proteome. While low protein yields were observed for all protocols, TCA/acetone precipitation followed by phenol extraction and SDC-FASP yielded the highest number of identified proteins. The total ion chromatograms (TIC) also confirmed the low peptide ion signals indicating low protein concentrations. Comparative analysis of leaf and gall wasp-modified tissues revealed different proteomic profiles. The leaf tissues were rich in proteins related to photosynthesis, energy metabolism and stress responses, while gall wasp-modified tissues had proteins related to nutrient acquisition, developmental regulation and immune modulation, reflecting the physiological effects of gall production on the host
Health literacy of hospitalized patients suffering from diseases of the gastrointestinal tract
No full textCilj: utvrditi razinu zdravstvene pismenosti i prediktore zdravstvene pismenosti kod gastroenteroloških bolesnika.
Izvori podataka i metode: Istraživanje je provedeno u Kliničkom bolničkom centru Split, na Zavodu za gastroenterologiju i hepatologiju. Sudjelovalo je 250 ispitanika, koji su popunili papirnati upitnik koji se sastojao od dvije dijela: općih podataka o bolesniku i modificiranog Europskog upitnika za procjenu zdravstvene pismenosti (HLS-EU-16). Za obradu podataka korišteni su deskriptivna statistika i Hi kvadrat test.
Rezultati: Rezultati istraživanja su pokazali da više od 80 % ispitanika ima adekvatnu i izvrsnu zdravstvenu pismenost (47,2 % adekvatnu, a 36,5 % izvrsnu zdravstvenu pismenost). Dob (30-59 godina), duljina trajanja bolesti, liječenje u Dnevnoj bolnici, mišljenje o posjedovanju znanja o svoj bolesti i odlasci na preventivne zdravstvene preglede su prediktori koji su statistički značajno povezani s razinom zdravstvene pismenosti bolesnika oboljelih od bolesti gastrointestinalnog trakta. Rezultati su također pokazali da spol, razina obrazovanja, izvori prikupljanja informacija o svojoj bolesti i provjeravanje informacija s interneta kod zdravstvenih djelatnika nisu statistički značajno povezani s razinom zdravstvene pismenosti.
Zaključak: Razina zdravstvene pismenosti hospitaliziranih gastroenteroloških bolesnika u cjelini je zadovoljavajuća, što potvrđuje prvu istraživačku hipotezu. Provedeno istraživanje naglašava važnost
poboljšanja zdravstvene pismenosti kao ključnog čimbenika za poboljšanje zdravstvenih ishoda i smanjenje troškova zdravstvene skrbi.Objective: to determine the level of health literacy and predictors of health literacy in gastroenterological patients.
Data sources and methods: The study was conducted at the University Hospital Center Split, at the Department of Gastroenterology and Hepatology. 250 respondents participated, who filled out a paper questionnaire consisting of two parts: general patient data and the modified European Health Literacy Assessment Questionnaire (HLS-EU-16). Descriptive statistics and the Chi-square test were used to process the data.
Results: The results of the study showed that more than 80 % of the respondents had adequate and excellent health literacy (47.2 % adequate and 36.5 % excellent health literacy). Age (30-59 years), duration of illness, treatment in a Day Hospital, opinion about having knowledge about one's illness and visits to preventive health examinations are predictors that are statistically significantly associated with the level of health literacy of patients with gastrointestinal diseases. The results also showed that gender, level of education, sources of information about their illness, and checking information from the Internet among healthcare professionals were not statistically significantly associated with the level of health literacy.
Conclusion: The level of health literacy of hospitalized gastroenterology patients is generally satisfactory, which confirms the first research hypothesis. The conducted research emphasizes the importance of improving health literacy as a key factor for improving health outcomes and reducing healthcare costs
Calculation of the Reinforced Concrete Residential Building and Verification of the Possibility of Applying Seismic Isolation with a Layer of Stone Pebbles under the Foundations : Master's Thesis
No full textU radu je prikazan proračun nosive konstrukcije armiranobetonske stambene zgrade. Zgrada se sastoji od prizemlja i dva kata. Proračun i dimenzioniranje nosivih elemenata konstrukcije je provedeno u skladu sa Europskim normama pomoću računalnog programa SCIA Engineer 21.1. Proračunate su rezne sile za temelje, ploče i zidove koji su sukladno tome i armirani. Projekt sadrži tehnički opis konstrukcije, proračun nosivih elemenata te karakteristične građevinske nacrte.Structural analysis of reinforced concrete residential building are presented in this work. The building consist of ground floor and two stories. Calculation of structural elements was performed in accordance with European standards using structural analysis software SCIA Engineer 21.1. Internal forces for the foundations, slabs and walls were calculated and reinforced accordingly. The project is consisted: technical description, calculation of structural elements and characteristic structural drawings
Strukturalna karakterizacija, antimikrobna aktivnost i toksičnost antimikrobnih peptida identificiranih u trakavice (Taenia solium Linnaeus, 1758)
No full textAll living organisms are constantly threatened by large number and great diversity of pathogens which is why all of them synthesize antimicrobial peptides (AMPs) as a first line of defense as a part of their innate immunity. These molecules show direct antimicrobial and/or immunomodulatory properties which make them a potential source of new therapeutics during global antimicrobial resistance crisis. As one of the most successful animal groups in nature, parasitic helminths (worms) found different ways to overcome and avoid expulsion by their host’s immune system. One of them is by secreting different immunomodulatory molecules one of which are also AMPs. Helminth’s coevolution with the host gut microbiome and reciprocal adaptation which favors development of compounds minimally invasive to the host make helminthic AMPs especially interesting for further investigation. The aim of this thesis was to characterize three potential AMPs from tapeworm (Taenia solium). That included bioinformatical analysis, structural characterization by CD spectroscopy, determination of antimicrobial activity and subsequent mode of action by propidium iodide (PI) uptake assay using flow cytometry. Cytotoxic activity was tested with MTT assay and late confirmed with PI uptake assay using flow cytometry. Two out of three peptides showed potent antibacterial activity with borderline levels of toxicity which should be addressed if the peptides undergo further studies for potential therapeutical applications.Svi živi organizmi konstantno su ugroženi velikim brojem i velikom raznolikošću patogena, zbog čega sintetiziraju antimikrobne peptide (AMP) kao prvu liniju obrane i dio svog urođenog imunološkog odgovora. Ove molekule pokazuju izravno antimikrobno i/ili imunomodulacijsko djelovanje što ih čini potencijalnim izvorom novih lijekova tijekom globalne krize razvoja rezistencije na antibiotike. Kao jedna od najuspješnijih životinjskih skupina u prirodi, parazitski crvi su pronašli različite načine da nadvladaju i izbjegnu imunološki sustav svog domaćina. Jedan od njih je izlučivanje različitih imunomodulacijskih molekula kao što su npr. antimikrobni peptidi. Koevolucija parazitskih crva s crijevnim mikrobiomom domaćina i recipročna prilagodba koja pogoduje sekreciji minimalno invazivnih spojeva čini ove antimikrobne peptide posebno zanimljivima za daljnje istraživanje. Cilj ovog rada bio je istražiti biološku aktivnost tri potencijalna AMP-a iz trakavice, Taenia solium. To je uključivalo bioinformatičku analizu, strukturnu karakterizaciju CD spektroskopijom, određivanje antimikrobne aktivnosti i mehanizma djelovanja koristeći propidij jodid i protočnu citometriju. Citotoksična aktivnost ispitana je MTT testovima i kasnije potvrđena koristeći propidij jodid (PI) i protočnu citometriju . Dva od tri peptida pokazala su potencijalno antibakterijsko djelovanje s graničnim razinama toksičnosti koju bi trebalo poboljšati ukoliko se peptidi budu dalje istraživali u svrhu potencijalne terapijske primjene
Glucosinolate profile and spectroscopic analysis of isolated desulfoglucosinolates from croatian wallflower (Erysimum croaticum) : diploma thesis
No full textGlukozinolati su specijalizirani biljni metaboliti usko povezani s porodicom Brassicaceae. Struktura se sastoji od β- D -glukopiranoze i O-sulfatirane (Z)-tiohidroksimatne skupine te promjenjivog bočnog lanca. Djelovanjem enzima mirozinaze nastaju razgradni produkti, poznati po brojnim biološkim aktivnostima, od kojih je najzanimljivije njihovo antikancerogeno djelovanje. U ovom radu određen je glukozinolatni profil hrvatskog šeboja (Erysimum croaticum), endemske biljke s Biokova, korištenjem UHPLC-DAD-MS/MS tehnike. Glukozinolati su izolirani u desulfatiranom obliku pomoću ion- izmjenjivačkih kolona prema metodi ISO 9167-1. Analizom MS2 i UV spektara dobivene su kvalitativne i kvantitativne informacije o glukozinolatnom profilu biljke. Ovaj profil je jedinstven za sve biljne organe i sastoji se od dva glukozinolata: glukorafenina i (3E)-4-(metilsulfanil)but-3-enil-glukozinolat. Cvat sadrži najveću koncentraciju glukozinolata (679,74 µmol/g suhog biljnog materijala), dok stabljika ima najmanju (123,85 µmol/g suhog biljnog materijala). Glukorafenin je dominantan u većini biljnih organa, osim u korijenu. Dva analizirana desulfoglukozinolata imaju dovoljno različito vrijeme zadržavanja što omogućava odvajanje na dvije frakcije koje sadržavaju pojedinačne desulfoglukozinolate. Strukture izoliranih desulfoglukozinolata su potvrđene NMR spektroskopijom.Glucosinolates are specialized plant metabolites closely related to the Brassicaceae family. The structure consists of β- D -glucopyranose and O-sulfated (Z)-thiohydroximate group and a variable side chain. Degradation products are produced by the action of the enzyme myrosinase, and are known for numerous biological activities, with their anticarcinogenic effects being the most interesting. In this study, the glucosinolate profile of Croatian wallflower (Erysimum croaticum), an endemic plant from Biokovo, was determined using UHPLC-DAD-MS/MS technique. Glucosinolates were isolated in desulfated form using ion-exchange columns according to the ISO 9167-1 method. Qualitative and quantitative information about the plant's glucosinolate profile was obtained by analyzing MS2 and UV spectra. This profile is unique for all plant organs and consists of two glucosinolates: glucoraphenin and (3E)-4- (methylsulfinyl)but-3-enyl-glucosinolate. The flower contains the highest concentration of glucosinolates (679.74 µmol/g of dry weight), while the stem has the lowest (123.85 µmol/g of dry weight). Glucoraphenin is dominant in most plant organs, except in the root. The two analyzed desulfoglucosinolates have sufficiently different retention times allowing separation into two fractions containing individual desulfoglucosinolates. The structures of isolated desulfoglucosinolates were confirmed by NMR spectroscopy