PubliCatt
Not a member yet
128119 research outputs found
Sort by
Live cell optical super-resolution microscopy of dystroglycan mutants as a model for dystroglycanopathies in multiple cell lines
Full text linkIntroduction Dystroglycan (DG) is an adhesion complex comprising two subunits, alpha-DG and beta-DG, which interact non-covalently at the plasma membrane. As a component of the dystrophin-glycoprotein complex DGC, DG plays a crucial role in linking the cytoskeleton to the surrounding basement membranes. Rare primary point mutations in the DAG1 gene have been identified in patients with various forms of neuromuscular dystrophy, ranging in phenotype from mild to severe.Methods To gain a deeper understanding of the molecular mechanisms underlying these pathologies, we have designed a series of chimeric GFP-tagged full-length alpha/beta-DG constructs and expressed them in three different cell lines (U-2OS, HEK-293T and C2C12). Wild-type DG constructs were compared to their counterparts carrying pathologic missense mutations previously described in patients, namely, L84F, T190M and C667F and with the mutant I591D, i.e., the topological equivalent of V567D identified in zebrafish.Results Live super-resolution fluorescence microscopy showed that the C667F mutant is retained within the ER/Golgi while the T190M and wild-type proteins are correctly localized to the plasma membrane in all 3 cell lines. The L84F mutant exhibits a delay in trafficking to the plasma membrane in two of the cell lines, while localizing strongly at the plasma membrane in the high-expression HEK-293T cells. Similarly, the I591D mutant accumulated at the plasma membrane in the HEK-293T cells, in contrast to the clear retention in the endoplasmic reticulum/Golgi apparatus observed in U-2OS and C2C12 cells.Discussion Our data demonstrate the importance of using a range of different cell lines for a comprehensive study of DG mutants or variants by live cell optical super-resolution microscopy
Istituzioni di diritto pubblico
No full textI. State, Constitution and European Union. I.1 Law and public law. I.2 Form of State I.3. The constitutions and the Italian Constitution. I.4 European Union and EU sources (Treaties, regulations and directives). II. Constitutional Rights. II.1 Constitutional freedoms and their protection II.2 Freedom of economic initiative and its limits. II.3 Protection of competition. II.4 Equality II. 5 Social rights. III. State, regions and local authorities. III.1 Statutory autonomy of the Regions. III.2 Regional laws. III.3 Financial autonomy of Regions and Local Authorities. IV. Form of Government. IV.1 Form of government and political rights. IV.2 Electoral systems. IV.3 State bodies. IV.4 Economic policy. V. Guarantees. V.1 Judiciary and special judges. V.2 Constitutional Court
Introduzione
No full textAlla galleria del realismo politico possono essere ricondotti pensatori tra loro molto lontani, da Tucidide a Sant’Agostino, da Machiavelli a Max Weber, da Thomas Hobbes a Carl Schmitt. A contrassegnare questa tradizione sono soprattutto due elementi: l’idea che la “natura umana” rappresenti un dato costante e la convinzione che una simile “natura” renda gli esseri umani perennemente insoddisfatti della loro condizione e tra loro irrimediabilmente in conflitto. Proprio in virtù di tale antropologia negativa, i realisti ritengono così che gli esseri umani puntino sempre a perseguire la sicurezza, a estendere la loro ricchezza e a difendere il loro onore. Il volume intende invece esaminare più in profondità questo pessimismo antropologico. E, in particolare, si propone di mostrare come, dietro un’apparente coerenza, si nascondano divergenze piuttosto nette. L’obiettivo è dunque esplorare le molteplici antropologie del realismo politico, riconoscendo e distinguendo le diverse modalità con cui è stata rappresentata e spiegata quell’inquietante “natura” – più o meno invariante – che conduce gli esseri umani a desiderare il potere e a inseguire la gloria
VERSO NUOVE STRATEGIE DI PREVENZIONE DEI REATI? IL CONTRUBUTO DELLA RESPONSIVE REGULATION E DELLA RESTORATIVE JUSTICE
No full textPremessa una ricostruzione della letteratura sulla prevenzione dei reati e una rivisitazione critica dei principali limiti ed «effetti collaterali» della giustizia punitiva, la tesi valorizza la dinamica persuasiva che ruota attorno al precetto penale e la sua carica motivazionale a sostegno di libere scelte conformi da parte dei consociati e indaga il contributo della responsive regulation e della restorative justice come strumenti di promozione della conformità alle norme ex ante ed ex post.
Il quadro teoretico delineato ha condotto a rileggere la compliance penale, di cui al d.lgs.
231/2001, e la giustizia riparativa, di cui al d.lgs. 150/2022, quali scelte di politica criminale in cui è possibile rinvenire tracce di una ‘special-general’ prevenzione ‘persuasiva’ in grado di orientare il «processo genetico delle regole» e il «meccanismo di manutenzione delle stesse» al fine di prevenire la commissione dei reati o di rimuoverne o di correggerne le cause, apprendendo «dagli eventi critici» e (ri)attivando un circolo virtuoso «all’insegna» della legalità.After a thorough review of the existing literature on crime prevention and a critical assessment of the limitations and unintended consequences of punitive justice, the thesis underlines the persuasive dynamics that revolve around the penal precept and its motivational charge in support of free conforming choices by citizens. It also explores the contribution of responsive regulation and restorative justice as instruments to promote ex ante and ex post compliance.
The theoretical framework outlined has led to a reinterpretation of criminal compliance (d. lgs 231/2001) and restorative justice (d. lgs 150/2022) as choices of criminal policy in which it is possible to discern the intertwining of 'general-special' preventive and 'special-general' preventive effects of a mature interlocution between those who produce the rule and those who are called upon to respect it.
This intertwining facilitates the orientation of the 'genetic process of the rules' and the 'maintenance mechanism of the same' in order to prevent the commission of crimes or to eliminate or correct their causes. It also makes it possible to learn 'from critical events' and to (re)activate a virtuous circle 'in the name' of legality
Elogio della “terza via” per rilanciare la cultura politica
No full textUn progetto di cui si parla da decenni, un’ipotesi che ha manifestato fragilità, ma che oggi è possibile recuperare in un contesto post-ideologico. Il personalismo di Mounier e Olivetti e il meridionalismo di Compagna. Un ruolo propositivo degli intellettuali
Lettere inedite di Eugenio Montale a Elena Croce: poesia, vita culturale, impegno civile
No full textThe article sheds light on the human and literary relationships between Eugenio Montale and Elena Croce by analysing the unpublished letters exchanged between them from 1946 to 1981. The missives, an edition of which is provided here according to the autographs, allow us to understand some remarkable aspects of the biography and work of the two correspondents. First of all, Montale’s collaboration with cultural magazines founded by Croce, such as «Aretusa» directed by Francesco Flora and Marguerite Caetani’s «Botteghe Oscure», where some rhymes of the Ligurian poet appeared in their first edition. The bond between Montale and Caetani, moreover, can be better defined thanks to these unpublished documents, beginning with the poet’s attendance at the cultural circle “Il ritrovo”, founded by Elena with the princess of Bassiano in the newly liberated Rome of 1944. Croce also played a significant role in putting the poet in contact with some of his important Anglo-Saxon translators: the English journalist and writer Sylvia Sprigge, the American Edith Farnsworth and the professor at the University of Texas William Arrowsmith. Elena herself, moreover, anthologized and made commentaries on Montale’s works (in her books and in the cultural programs of Radio RAI) and she used to send him her own volumes, which were sometimes accompanied by handwritten dedications. For his part, Montale repeatedly expressed his support for the environmentalist battles fought by Croce in the Seventies, with implications that link the letters with his production in verse. But there are many other insights and characters that mark both the correspondence and this long intellectual friendship: the interest in T.S. Eliot and German Romanticism, Linuccia Saba, Carla and Giovanni Macchia, Adriano Olivetti, Desideria Pasolini, Luigi Magnani, Giovanni Spadolini, the editorial entourage of «Il Mondo», «L’Immagine» and «Corriere della Sera», without forgetting the pervasive memory of Elena’s father, Benedetto Croce
Il paradosso dei lavoratori della cura: sono decisivi ma ai margini del welfare
No full textL'articolo descrive la progressiva affermazione del codice della cura nella gestione delle risorse umane e nelle pratiche di welfare aziendale, sottolineando i rischi di una paradossale discriminazione proprio dei lavoratori e delle lavoratrici della cur
Concentrating normalized solutions to planar Schrödinger–Poisson system with steep potential well: critical exponential case
No full textIn this paper, we study the following class of planar Schrödinger–Poisson problems (Formula presented.) where a>0, μ∈R is an unknown parameter appearing as a Lagrange multiplier, λ,γ,κ>0 are parameters, V∈C(R2,R+) admits a potential well Ω≜intV-1(0) and f is a continuous function having critical exponential growth at infinity in the Trudinger-Moser sense. Owing to some technical tricks adopted in Alves and Shen (On existence of positive solutions to some classes of elliptic problems in the hyperbolic space, Submitted for publication), Shen and Squassina (Existence and concentration of normalized solutions for p-Laplacian equations with logarithmic nonlinearity, http://arxiv.org/abs/2403.09366), we are able to obtain the existence and concentrating behavior of positive normalized solutions for sufficiently large λ using variational method
Investor networks and social innovation: A stakeholder network analysis of Social Impact Bonds
No full textSocial Impact Bonds (SIBs) have emerged as a novel paradigm within social finance, harnessing private capital to address social issues. This collaborative model convenes diverse stakeholders, including government agencies, service providers, and investors, to achieve predefined social outcomes linked to financial returns. A core component of SIBs is the investor network, which encompasses institutional investors, philanthropists, and impact investors. This network plays a key role in influencing project financing, intervention strategies, and governance structures. This study leverages stakeholder network theory to explore these dynamics. By applying network analysis, we map out how stakeholders interact, depend on one another, and share information. We analyse metrics like centrality and clustering to understand how the network structure impacts investor behaviour and project success. Our findings show that investors with higher betweenness centrality have a greater influence, linked to improved project performance and stronger social impact outcomes. This research offers fresh evidence of how network dynamics affect SIB results, providing valuable insights for improving investor involvement and network efficiency in future social finance projects
Italy
No full textWhen Shelley resolved to leave England for Italy with his family, he conceived his expatriation as a voluntary exile. Yet, for several months after their arrival, the Shelleys criss-crossed the peninsula like the Grand Tourists of old, visiting all its major cities and a few minor destinations, which Shelley described at length in his correspondence and often evoked in his poetry. A peculiarity of Shelley’s travel letters is his ambivalent attitude towards Italy, revealed by his constant juxtaposition of the magnificent beauty of its art and nature and the equally striking spectacle of the Italians’ degradation. However, as the first independence movements raised the promise of the country’s political and cultural resurgence, Shelley started to develop a greater appreciation of its inhabitants. At the same time, having finally settled, he turned his attention away from the wonders and contrasts of Italy to celebrate the simple life of his exile community