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無葉緑素藻類プロトテカの感染症について
Get PDFProtothecosis, a rare infectious disease, is mainly caused by Prototheca wickerhamii and Prototheca zopfii, but in humans, most cases have been reported to be caused by P.wickerhamii. In this article, the characteristics of these two types of Prototheca are described based on the microbiological and histopathological findings, and five possibilities are proposed and discussed as to why there are so few case reports of P.zopfii. It is expected that providing knowledge about Prototheca and raising awareness of the infectious disease will help in future testing and diagnosis and promote the early establishment of treatment methods.technical repor
Effects of high-intensity interval walking training on muscle strength, walking ability, and health-related quality of life in people with diabetes accompanied by lower extremity weakness : A randomized controlled trial
No full textAims/Introduction: This study examined the effects of high-intensity interval walking training (IWT) compared to moderate-intensity continuous walking training (CWT) on muscle strength, walking ability, and health-related quality of life (QOL) in people with diabetes accompanied by lower extremity weakness.
Materials and Methods: People with diabetes accompanied by low isometric knee extensor strength using a simple manual dynamometer (n = 50) were screened and randomly divided into 2 groups: CWT (n = 25) and IWT (n = 25). Both groups were instructed by a physical therapist to perform walking training with the goal of 120 min/week over a 5-month period. The primary outcome, mean change of isometric knee extensor strength, and secondary outcomes, such as gait speed and health-related QOL, were measured at baseline and the end of the intervention.
Results: At the end of the intervention, there was no significant difference in the degree of change in isometric knee extension strength between the two groups. However, there was a significant increase in changes in gait speed and physical QOL in the IWT group (gait speed, P < 0.01; physical QOL, P < 0.05).
Conclusions: The present study showed that IWT for people with diabetes accompanied by lower extremity weakness did not improve knee extension muscle strength compared to CWT but did improve walking ability and physical QOL.journal articl
A 3D Cell-Culture System That Uses Nano-Fibrillated Bacterial Cellulose to Prepare a Spherical Formulation of Culture Cells
No full textA 3-dimensional (3D) cell culture is now being actively pursued to accomplish the in vivo-like cellular morphology and biological functions in cell culture. We recently obtained nano-fibrillated bacterial cellulose (NFBC). In this study, we developed a novel NFBC-based 3D cell-culture system, the OnGel method, and the Suspension method. HepG2 human liver cancer cells were cultured via these methods and formed spherical formulations in the optimized condition, 1.0% (w/v) of NFBC in the OnGel method, and 0.06–0.10% (w/v) of NFBC in the Suspension method. Non-cancerous cells such as human-induced pluripotent stem (iPS) cells and human mesenchymal stem cells (MSCs) also formed spherical formulations. It is noteworthy that both the size and cell viability of spheroids prepared via these methods were comparable to those cultured using commercially available 3D cell-culture systems. Both OnGel and Suspension methods are less complicated than the existing 3D cell-culture systems, which is an invaluable advantage for the preparation of cancer spheroids. The NFBC-based 3D cell-culture systems introduced here show great promise as a tool to prepare cultures for cell-derived spheroids for the progress of both in vitro and in vivo studies of the biological functioning of cells.journal articl
Intraoral Myeloid Sarcoma in a Patient with Myelodysplastic Syndromes : Case a Report
Get PDFMyeloid sarcoma (MS) is a rare extramedullary tumor mass consisting of mature or immature myeloblasts. MS occurrence is mainly associated with the onset of acute myeloid leukemia (AML) and has a poor prognosis. MS also occurs, to a lesser extent, in myelodysplastic syndromes (MDS). The progression of MDS depends on the percentage of myeloblasts in peripheral blood or bone marrow. The transformation to AML is defined as the presence of more than 20% of myeloblasts. Herein, we report an extremely rare case of intraoral MS that arose in the hard palate over the course of the leukemic transformation of MDS. A 72 years-old Japanese man with a medical history of MDS was referred to our department because of continuous bleeding from the teeth extraction sockets. He had a gingival mass resembling a palatal abscess in his hard palate and underwent tooth extraction and puncture of the mass. Blood tests revealed that the proportion of myeloblasts increased up to 9% at that time. An incisional biopsy was carried out. Analysis of the histopathological findings showed aggregation of large, blast-like cells under the gingiva. These cells were positive for myeloperoxidase, naphthol AS-D chloroacetate esterase, CD68, and CD117 and negative for CD3, CD20, CD79a, and CD138. The Ki-67 labeling index was about 80%. The histopathological diagnosis was MS. Because stage progression of MDS was suspected, the hematologist performed bone marrow aspiration. The percentage of myeloblasts in the bone marrow was 26%, and the diagnosis of transformation to AML was confirmed. After one course of remission-induction chemotherapy by low-dose cytarabine, aclarubicin, and granulocyte-colony stimulating factor, the myeloblasts disappeared from the peripheral blood and bone marrow, the intraoral tumor regressed completely, and no further bleeding was observed. Twelve months after the chemotherapy, the patient died from a pneumonia.journal articl
