Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry)
Not a member yet
300 research outputs found
Sort by
MENUJU LABORATORIUM ANALISIS LlNGKUNGAN TERPADU DAN TANTANGAN MASA DEPAN
No full textAn idea on an integrated environmental analytical laboratoryand its challenges for the future were discussed. A generalanalytical laboratory equipped with conventional and instrumentalmethods could be improved to environmental laboratory byaddition of sampler eguipment. These include water sampler, impinger for sampling of ambient air, stack gas sampler andleaching test (TCLP) , all of these have been made locally andtherefore easily purchased with low price. Laboratory accreditationwas also discussed for improving the quality of laboratory as areliable environmental data generator. Accreditation requirementssuch as registration, methods of analysis, quality control, physicalcondition of lab oratory, equipment and instrument were explained.In addition, other challenges in research were pointed out on thearea of semi-micro techniques, sensor, micro-instruments,automation and telemetric system. Experiences showed thatenvironmental analytical laboratory had good challenges forbusiness. Analytical services, development of monitoring systemand training were identified as prospective business in Indonesiaparticularly in East of Java
Studi Kromatografi Lapis Tipis Preparatif pada Pelat Silika dan Kromatografi Cairan Kinerja Tinggi pad a Kolom C18 dari Senyawa-Senyawa Hasil Biokonversi Solasodine
No full textThe separation of solasodine bioconversion products-after fermentation with Mycobacterium phlei DSM 43286 has been carried out, using preparative thin layer chromatography on silica plate with chloroform-ethanol (48:1) mixture as an eluent. Chromatographic cross check of the compound being separated has also been done. In addition to the silica stationary phase, the separation of bioconversion products using CIS has also been explored. Solanesol and six derivatives of androstane or androstene standards could be well separated on Ci8 plate using methanol-chloroform (4:1). For the High Performance Liquid Chromatography (HPLC) separation on C18 column, the mixture of methanol-water was used instead of methanol-chloroform, since with the latter eluent, compounds being separated were eluted together with the solvent peak. The optimum resolution of solasodine bioconversion products could only be attained when the gradient elution technique using the mixture of methanolwater was used
PRODUKSI GLUKOAMILASE DARI RHIZOPUS ORVZAE L16 PADA MEDIA PATI SAGU (METROXYLON) YANG MENGANDUNG EKSTRAK TAUGE
No full textGlucoamylase production from Rhlzopus oryzae L16 has been carrried out in a fermentation medium using sago (Metroxylon sp) starch. Various levels of mung bean sprout extract (1% - 5%) was added into the medium as vitamin source. The fermentation process was carried out at 30C in aerobic condition with an agitation rate of 120 rpm and for five days. The results of the fermentation was compared with an other fermentation medium containing malt extract 3%. The glucoamylase specific activity of 4500 Unitslg protein, starch consumption of 62.5% and biomass produced of 2.93 g dry weighill: medium were demonstrated with the latter medium. It was found that media which contained 4% mung bean sprout extract had maximum glucoamylase specific activity of 19720 Unitslg protein at day-3. The enzyme activity was assayed at 55C with incubation time 10 minutes. At this third day of fermentation the starch utilization reached 55.5% and the biomass production was 3.12 g dry weight/L medium
KARAKTERISASI DAN UJI-PENGOLAHAN (TREATABILITY TEST) AIR LIMBAH TEKSTIL INTI SARI SECARA BIOLOGI
No full textCharacterization and treatability test of textile wastewater using biological process were studied. Samples of the wastewater were obtainedfrom a local blue-jeans textile industry in Bandung. Results of chemical analysis indicated that beside the textile wastewater showed a high pH value, it contained chemical pollutant in high concentrations. It was found that the textile wastewater of blue jeans has a pH value of 12.6, deep blue coloured with optical density of 0.80 - 0.88; high in solid concentrations with the concentration of soluble solids, suspended solids and ash content of respectively 4526 - 4897 mg/l, 2205 - 2412mg/l, and 2704 - 3074 mg/l; COD of 376 - 1538 mg/l and BOD5 of 143 - 575 mg/l. The ratio of BOD5/COD was about 0.32 - 0.45 with an average value of 0.38.The concentration of organic materials varied widely from sample to .sample, with a value of the BOD5/COD ratio of less than 50%. Tretability test of textile wastewater using shake-flask technique showed that the textile wastewater could be treated successfully by aerobic biological process when a proper balance of nutrients and suitable organisms were available. Chemical flocculation pre-trentment was found to lower the rate of biological degradation of organic materials contained in the textile wastewater. It was found that after 3 days of incubation, the mean rate of organic degradation reached 174 mg/l/day for the textile wastewater which received no flocculation pretreatment with alum, and only 10 mg/l/day for that which received flocculation pre-treatment with alum. The addition of nutrient (nitrogen and phosphorous) to the textile wastewater without flocculation pre-treatment increased the mean rate of organic degradation from 174 mg/l/day to 240 mg/l/day after 3 days of incubation. It was also found the nutrient addition and bacterial inoculum to the textile wastewater without flocculation pre-treatment increased the mean rate of organic degradation from 174 mg/l/day to 247 mg/l/day, after 3 days of incubation.Treatability test of textile wastewater by a contlnouous culture technique using perforated-plate column showed that the COD of effluent was constantly dropped and achieved a stationary condition after 50 hours of the continous process, and at the stationary phase the continuous process produced effluent with COD values of less than 100 mg/l. Continuous process with a hydraulic retention time (0) of 2.0 day produced effluent with a COD value of 77 mgll. Reducing the hydraulic retention time did not much affect the COD value of the effluent, i.e 72 mgfl for 0 = 1.0 day, and 56 mgll for 0 = 0.5 day. This could probably due to the achievement of the maximum efficiency of treatment, t.e. about 71% for the hydraulic retention time of 0.5 - 2.0 day
HIDROGENOLISIS METILSIKLOPENTANA PADA KATALIS 0.3% Pt/Al2O3
Get PDFHydrogenolysis of methylcyciopentane (MCP) was carried out with 0.3% Pt/Al2O3 catalyst at 210 C and 235 C in a batch reactor. Chemisorption of hydrogen and oxygen on the catalyst had also been measured. The mean value of platinum dispersion on the catalyst support measured by chemisorption technique was D = 84%. The product distribution of hydrogenolysis of MCP, namely n- hexane : 2- methylpentane : 3-methylpentane was found to be 15: 55: 30 at 210 DCand 20: 50: 30 at 235 C. The adsorption of MCP molecule at the boundary area between the metal particle and the support material was assumed to be the determining factor for that distribution profile. The activation energy (Ea) of the hydrogenolysis of MCP on this catalyst was 95 kJ/mol
PEMURNIAN DAN KARAKTERISTIK DEXTRANASE BACTEROIDES RUMINICOLA SUBSP BREVIS
Get PDFThe dextranase of B.ruminicola subsp. brevis from bovine rumen has high ability to degrade the D - (1,6) a-glucosidic linkages of dextran substrate. Purification of crude dextranase was done by using ion-exchange chromatography and electrophoresis. It was observed that the purification until ion exchange step by microcrystal cellulose column increased theactivity of dextranase by 400 fold. The purified dextranase was most active at pH 5.5 and 40C-45C. The enzyme was strongly inhibited by metal ions such as Cu++,Fe++ and Hg++. The major product in early stage of dextran hydrolysis which were analyzed by paper chromatography showed dextranase of B.ruminicola to be an endotype enzyme
BIOSENSOR UNTUK ANALISIS UREA BERDASARKAN PADA APLIKASI ENZIM UREASE DAN ELEKTRODA TUNGSTEN
Get PDFThis study describes the development of a potentiometric biosensor for the determination of urea. The sensor was constructed by combining a tungsten wire with an enzyme, urease, physically immobilized in polyvinylchloride (PVC). The fabrication of the sensor was carried out by coating a tungsten wire with the immobilized urease. The changes in pH resulted from the enzyme-catalyzed hydrolysis of urea is measured with the tungsten electrode and shown to be proportional to the log of concentration of urea in sample solutions over a limited concentration range. Urea Sensor prepared in this study gives an almost linear response with the log of concentration in the range of 0.1 - 10.0 mM, and the calibration slope wasfound to be 52 mV Vper decade change in urea concentration
Simple Purification of Vetiver Oil by Multiglass Plate System for Quality Improvement
Get PDFVetiver oil is one of essential oil from Indonesia which no synthetic substitute is available. The drawback of vetiver oil from Indonesia is smoky odor and dark appearance. To increase added value of Indonesian vetiver oil, therefore it is need method with cheap production, simple and easy to operate. Multiglass plate system (MGS) is one method to increase added value of vetiver oil. The aim of this research is to improve the vetiver oil quality in terms of appearance, odor and the physicochemical properties by MGS method and also optimize the operation condition on the vetiver oil yield and total vetiverol contents. The process parameters used on this research are to optimize the flow rate, angular and amount of glass plate. As a result, was obtained the optimum condition in 0.38 ml/sec of flow rate, 3o of glass plate angular and 5 of glass plate to increase 15% of total vetiverol contents, while the yields decreased until 5.6%. Refined vetiver oil by MGS are almost meet fulfill with Indonesia National Standard number 06-2386-2006 except total vetiverol contents and odor. Even though, the total vetiverol content increased and the odor became slightly smoky compare to raw material. Based on the results, MGS method can give higher purification of vetiverol of increase vetiver oil quality with easy to handle, low cost operation and also can moved to anywhere due to portable unit and mobile system
Proses Dekomposisi untuk Pembuatan Serbuk Alumina dari Aluminium Format
No full textThe high purity excellent chemical stability and mechanical properties, is quite important for alumina ceramic products. The alumina ceramic products was made from aluminium formate in this study. Decomposition of organic salts is a useful method for preparation of pure and fine ceramic powder without using of expensive metal alkoxides and without producing air pollution. The aluminium formate alt was prepared from reagent grade AlCl3.6H2O,AlCl3.6H2O was dissolved in H2O and then NH4OH was added to the solution. The white precipitates formed were washed with distilled water repeatedly, then dissolved in formic acid solution. Alumina powders prepared by decomposition of aluminium formate had high chemical purity and had small size of agglomerates with a narrow size distribution. The spesific surface area and the equivalent spherical diameter calculated from the value of spesific surface area were 60 m2/gr and 0.03um. The value of equivalent spherical diameter of Al2O3 powder in cumulative mass percent for finer particle were diameter (10%) of 1.7um and diameter (50%) of 5,5um. The average diameter of particles was 5.5um. For the alumina powders which was prepared by docomposition of aluminium formate, the x- Al2O3 and x- Al2O3 did not appear as intermediate phase between y- Al2O3 and a- Al2O3 when calcinated from 800C to 900C .However a- Al2O3 phase appeared at 800C, so that this could reduce the transition temperature from y- Al2O3 to a- Al2O3. The single phase of a- Al2O3 was obtained after calcinating at 1100C or more for 2 hours