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Proučavanje degradacije PFOA upotrebom mikrobne gorivne ćelije na bazi sulfat-redukujućih bakterija
No full textZagađenje gotovo svih prirodnih sredina širom sveta predstavlja jedan od vodećih problema za javno zdravlje, što zahteva razvoj tehnika za rešavanje ovog problema. Jedna od tehnika su i mikrobne gorivne ćelije u sedimentima koje mogu tretirati kontaminante u vodenim sredinama.
Cilj ovog rada je ispitivanje sistema mikrobnih gorivnih ćelija i uticaj sulfat-redukujućih bakterija kod uklanjanjanja perfluorovanih jedinjenja iz sedimenta. U tu svrhu konstruisana su dva različita jednokomorna sistema sa rečnim sedimentom mikrobnih gorvinih ćelija. Prvi sistem je predstavljao kontrolnu ćeliju sa sulfat-redukujućim bakerijama, dok je drugi sistem pored mikroorganizama dodatno sadržao i perfluorovana jedinjenja, koja u ovom slučaju predstavljaju zagađujuću supstancu. U sklopu eksperimeta praćena je koncentracija perflorovanih jedinjenja tokom tretmana, kao i proces respiracije mikroorganizama uključenih u procese
Structural and functional characteristics of β-lactoglobulin/C-phycocyanin/starch composite gels induced by pressure
No full textHigh-pressure processing (HPP) has emerged as a key sustainable green alternative for food treatment, effectively preserving food's sensory and nutritional properties. This study investigated the potential of HPP to develop β-lactoglobulin (BLG) gels in the presence of starch and the bioactive blue protein C-phycocyanin (C-PC). Various compositions of binary (BLG/C-PC and BLG/starch) and ternary (BLG/C-PC/starch) systems were subjected to high-pressure (HP) conditions at 4,500 bar. BLG, C-PC, and starch concentrations were maintained at 180, 10, and 5 g/L. HP-induced hydrogels preserve C-PC colour and partial preservation of secondary and tertiary structures, as evidenced by visible absorption and CD spectroscopy. SAXS data at the high-Q range revealed that C-PC induces the unfolding of BLG within binary system gels. In contrast, the ternary system gel maintained the BLG tertiary structure better. C-PC and starch affected the nano and microstructures of BLG HP-induced gels by increasing the pore size, as demonstrated by SAXS (at low Q-range) and SEM analysis. This increase in pore size further influenced the resulting gels' rheological behaviour and texture profile. C-PC enhanced the protein solubility and antioxidant activity of the HP-induced gels, potentially boosting the bioactive and nutritional value of the developed gels.This is the peer-reviewed version of the article: Jovanovic, Z., Gligorijević, N., Annighöfer, B., Dudzinski, D., Lević, S., Pavlović, V., Nikolić, M., Brûlet, A., Assifaoui, A., Combet, S.,& Minić, S.. (2025). Structural and functional characteristics of β-lactoglobulin/C-phycocyanin/starch composite gels induced by pressure. in Food Hydrocolloids Elsevier., 171, 111791. [https://doi.org/10.1016/j.foodhyd.2025.111791]Supplementary information: [https://cherry.chem.bg.ac.rs/handle/123456789/7187
Energetics of steps in proton pumping mechanism of cytochrome C Oxidase
No full textCytochrome c oxidase (CcO) links the reduction of O2 to H2O with proton
pumping across the mitochondrial membrane, generating an electrochemical
proton gradient that conserves energy in aerobic cells [1]. The coupling of
protonation and redox reactions is essential in this process. Recent time
resolved optical and electrometric experiments have anticipated a sequence
of reaction steps for the proton translocation mechanism in CcO. DFT/
electrostatic calculations are employed to obtain energetics of the proton and
electron transfer reaction steps during the O→E transition. The energy
profile of the reaction mechanism is studied by examining how the redox
state of the metal centers, dielectric solvation effects, and membrane
potential gradient influence the energy levels and potential leakage of the
protein pump through the Glu242 gating site. Particular emphasis is made on
side reactions that may short-circuit the pump, resulting in a loss of proton
pumping, and how this may be avoided in natural biological systems [2-5].The authors thank the COST Action
CA21101 (COSY) and COST Action CA22143 (EuMINe) of the European
Community for their support
Effect of packaging type on antimony leaching into bottled water
No full textThe type of packaging material is a critical factor influencing the safety of bottled water. Polyethylene terephthalate (PET) and glass are the most commonly used materials, with PET being widely applied due to its light weight and durability. However, antimony oxide, used as a catalyst in PET production, can leach into water, raising potential health concerns. In contrast, glass is generally chemically inert and does not contribute to antimony leaching [1,2]. The aim of this study was to compare antimony leaching from PET and glass packaging under different storage conditions. Six commercial bottled water brands were analyzed at three temperatures (−20 °C, 25 °C, and 40 °C) using inductively coupled plasma mass spectrometry (ICP-MS). In addition, the total Sb content of PET bottles was determined. The results confirmed Sb leaching exclusively from PET packaging, with the highest concentrations detected at 40 °C, exceeding the EU/EPA guideline value of 5 µg/L. In contrast, glass packaging showed stable Sb levels under all storage conditions, with the only exception being bottles sealed with PET-based caps, where measurable Sb leaching was observed. These results confirm that antimony leaching is associated with PET packaging and that glass packaging remains essentially inert under the studied conditions
Metal Complexes with Hydroxyflavones: A Study of Anticancer and Antimicrobial Activities
No full textMetal chelation to bioactive small molecules is a well-established strategy to enhance thebiological activity of the resulting complexes. Among the widely explored structural motifs,the combination of prominent metal centers with naturally inspired derivatives has attractedconsiderable attention. One such promising platform is the flavone scaffold, derivedfrom flavonoids and studied since ancient times. Flavones are plant-derived compoundsknown for their diverse biological activities and health benefits. They exhibit significantstructural variability, primarily through backbone modifications such as hydroxylation.Importantly, coordination of metal ions to hydroxylated flavone cores often improvestheir natural bioactivities, including anticancer and antimicrobial effects. In this review,we summarize transition metal complexes incorporating hydroxyflavone (OH–F) ligandsreported over the past 15 years. We provide a concise overview of synthetic approachesand structural characterization, with a particular emphasis on coordination modes (e.g.,maltol-type, acetylacetonate-type, catechol-type, and others). Furthermore, we discussbiological evaluation results, especially anticancer and antimicrobial studies, to highlightthe therapeutic potential of these complexes. Finally, we suggest directions for the futuredevelopment of metal-based agents bearing hydroxyflavone moieties through several criticalpoints in terms of the accuracy, reproducibility, and relevance of biological studiesinvolving metal-based compounds
Computational Study of Interfacial Charge-Transfer Complexes Between ZnO and Thiol Analogs of Catechol
No full textInterfacial charge-transfer (ICT) complexes provide an effective strategy for extending the optical absorption of wide-bandgap oxides into the visible spectrum, a key requirement for enhancing photo-driven reactions. In this work, density functional theory (DFT) and time-dependent DFT (TD-DFT) calculations were employed to investigate the ICT complexes formed between ZnO nanoparticles and benzene derivatives, specifically catechol (CAT), 2-mercaptophenol (2-MP), and 1,2-benzenedithiol (1,2-BDT). The results show that ZnO complexes with catechol and 2-mercaptophenol display ICT transitions in the visible region, whereas the 1,2-benzenedithiol complex primarily absorbs in the ultraviolet range. This behavior is attributed to differences in Zn─O and Zn─S bonding interactions, with sulfur-containing ligands forming stronger bonds. Nevertheless, the absorptions of 2-MP/ZnO and 1,2-BDT/ZnO are blue-shifted compared to the CAT/ZnO system. Further modifications, such as ligand substitutions, could be necessary to optimize absorption near the solar spectrum's peak. Therefore, a crucial aspect of this study is the assessment of two sulfur-containing analogs of catechol, a well-established ligand for use in ICT-based applications. © 2025 Wiley-VCH GmbH
Supporting information for: "Exploiting the Versatile Reactivity of Aryldiazonium Tetrafluoroborate Salts in the Synthesis of New Isochromene-Fused CF3-Substituted Pyrazoles with Anticancer Potential"
No full textPyrazoles, and in particular their fluorinated derivatives, are valuable heterocyclic moieties in medicinal chemistry. Our research group recently developed a one-pot, two-step synthesis of pyrazolo[3,4-c]isochromenes, combining two privileged structures into a single hybrid molecule. Based on the preliminary anticancer screening results of these derivatives, herein, we designed a next-generation compound library focused on the derivatization of a specific portion of the 1-(trifluoromethyl)-3,5-dihydroisochromeno[3,4-c]pyrazole scaffold via late-stage functionalization. The diverse reactivity of aryldiazonium tetrafluoroborate salts was leveraged to achieve this and generate a small but varied compound library. The new series was tested for their anticancer properties and selected derivatives demonstrated pro-apoptotic activity in the human cervical carcinoma cell lines HeLa. The obtained results highlight these structures as valid candidates for further biological studies, while the streamlined synthetic route offers a facile approach to further structural analogs.The supporting information for: Andrijević, Ana; Marković, Milica; Selaković, Milica; Grozdanić, Nađa; Stanojković, Tatjana; Nikolić, Andrea M.; Opsenica, Igor M. "Exploiting the Versatile Reactivity of Aryldiazonium Tetrafluoroborate Salts in the Synthesis of New Isochromene-Fused CF3-Substituted Pyrazoles with Anticancer Potential" in Asian Journal of Organic Chemistry, 2025, 14, 9, e00315-, [https://aces.onlinelibrary.wiley.com/doi/10.1002/ajoc.202500315]Published article: [https://cherry.chem.bg.ac.rs/handle/123456789/7286
Analiza redoks parametara u serumu šest meseci nakon treće doze vakcine Sinopharm [Vero Cell] – Inaktivisane COVID-19 vakcine
No full textŠest meseci nakon primene treće doze Sinopharm [Vero Cell]-Inaktivisane COVID-19 vakcine, sprovedena je analiza redoks parametara kod vakcinisanih ispitanika sa i bez prethodne infekcije SARS-CoV-2 virusom. Rezultati su pokazali da osobe koje su preležale COVID-19 imaju značajno niže vrednosti serumskog albumina i DPPH aktivnosti, što ukazuje na smanjenu antioksidativnu sposobnost i prisustvo dugotrajnog redoks disbalansa u krvi ispitanika. Korelaciona analiza je potvrdila da su ukupni proteini i albumin u snažnoj pozitivnoj vezi kod ispitanika bez prethodne infekcije, dok je povezanost ova dva parametra bila slabija kod COVID-19+ grupe ispitanika, verovatno usled postinfekcijskih promena u sintezi i metabolizmu proteina. Višestruka regresiona analiza je pokazala da starija životna dob negativno utiče na nivo tiola i DPPH vrednosti, dok su albumin i ukupni proteini pozitivni prediktori antioksidativnog kapaciteta. Prisustvo komorbiditeta, naročito kardiovaskularnih, plućnih, bubrežnih i onkoloških bolesti, bilo je povezano sa smanjenim antioksidativnim statusom i povišenim markerima oksidativnog stresa
Research data for the manuscript: Size and end-group chemistry of linear polyethylene terephthalate oligomers matter in chemical risk assessment
No full textResearch data for the paper: Size and end-group chemistry of linear polyethylene terephthalate oligomers matter in chemical risk assessment. Volume size distribution of PET oligomer dispersions as obtained through laser diffraction recorded on the Mastersizer 3000 (Malvern Panalytical, Worcestershire, United Kingdom). This dataset includes results for PET oligomers dispersed in three different water-based dispersants containing either bovine serum albumin, ovalbumin or lysozym
Polypropylene micro- and nanoplastics affect the digestion of cow's milk proteins in infant model of gastric digestion
No full textInfants may ingest large quantities of micro- and nanoplastics (MNP) due to the heating and sterilization of the feeding bottles, which promotes the generation and migration of MNP into the milk or milk formula. The effect of MNP on the digestion of proteins, which are crucial for the growth and development of infants remain unknown. The current study investigated the in vitro digestion of cow's milk proteins (1 mg/mL) with or without polypropylene (PP) microplastics (MP, pristine or oxidized) (20 mg/mL and 63–180 μm) in simulated gastric fluids (SGF) using an infant (pH 5.0; pepsin activity; 268 U/mL) or adult (pH 3.0; pepsin activity; 2000 U/mL) digestion model at 37 °C for 5, 30, and 120 min. Secondly, the effect of the presence of agglomerated PP nanoplastics (NP, 10, 50, and 100 μg/mL) in milk on the in vitro digestion process using the infant model of gastric digestion was investigated. The profiles of protein digestion products, soft corona and hard corona, were analyzed using SDS-PAGE, and nLC-MS/MS, respectively. Cow's milk protein digestion with or without PP-MP was significantly slower in the infant compared to the adult model, and oxidation of the PP-MP enriched some proteins in the soft and hard corona. In the presence of agglomerated PP-NP, an additional decrease in the rate of milk protein digestion was observed, especially proteins of Mw between 18 and 20 kDa, presumably allergenic β-lactoglobulin. Irrespective of the type of PP, six different types of proteins, including casein α-S1, α-S2, β and κ, β-lactoglobulin, and α-lactalbumin, were present both in the soft and hard corona. Our results indicate a direct impact of PP-MNP on the rate of milk protein digestion in the infant model of gastric digestion. Aging of PP MP through oxidation and smaller size nanoplastics exert more pronounced effects on the digestion of cow's milk proteins in vitro. This suggests that PP-MNP could affect the biological functions of milk proteins and promote chronic health problems