7348 research outputs found
Sort by
Allergenic potency of various foods of mammalian origin in patients with α-Gal syndrome
No full textBackground The α-Gal syndrome (AGS) is an emerging allergy to mammalian food caused by IgE-mediated reactions to the carbohydrate galactose-α-1,3-galactose (α-Gal). Mammalian food sources contain α-Gal, but the amount differs. The objective of this study was to investigate the allergenic potency of various foods of mammalian origin among AGS patients. Methods Twenty-six AGS patients were included. Food extracts from innards, lean meats, processed meat products, milk, and whey were analyzed. Immunoblot, ELISA, immunofluorescence, and basophil activation test were used to determine the α-Gal content, characterize IgE binding, and assess foods' allergenicity. Results The determined amount of α-Gal, IgE reactivity to food extracts, and food extract potencies to activate patients' basophils correlated well with each other. Pork and beef kidney showed the highest allergenicity. Beef liver and bacon showed allergenicity comparable to that of lean meats. Game meat seemed to have a higher allergenic potency than meats from farm-raised animals. The processed meat products liver pâté and black pudding, despite lower α-Gal content, demonstrated moderate allergenicity. Milk showed the lowest allergenicity. IgE reactivity to food extracts was highly similar for all patients and strongly dominated by the α-Gal epitope. Conclusions The allergenic potency of mammalian meat depends on the origin of the meat, the different cuts, and type of processing, with innards posing the greatest risk to AGS patients. Even processed mammalian meat constitutes a risk. Dairy products show the lowest risk. This study highlights the importance of analyzing even more foods to improve the management of AGS
A highly selective ESIPT-mechanism-based, ratiometric fluorescent sensor for zinc ions
No full textZinc is crucial for human health, a fact that has prompted interest in developing efficient fluorescent probes for
its detection. Traditional approaches, however, face issues such as environmental interference and limited
selectivity. This has led to research focusing on ratiometric sensing and making use of the excited-state intramolecular
proton transfer (ESIPT) effect. Tetramic acid and its 4-methoxy analogue are significant structural
motifs in many natural products from various organisms, including those which can bind metal ions, such as zinc.
This study presents a new ratiometric fluorescent sensor for zinc that leverages ESIPT for high selectivity and
efficacy, and combines tetramic acid and pyridine substructures. Our probe exhibits a strong emission band at
515 nm upon excitation in polar aprotic solvents, and upon excitation at 360 nm a selective ratiometric response
to Zn2+ in acetonitrile, with distinct, blue-shifted emission maximum at 460 nm. This is accompanied by a color
change from green to turquoise, which is visible to the unaided eye under a UV lamp. Our sensor shows excellent
sensitivity, which is confirmed by a low detection limit of 1.26 × 10 -6 M. The binding of the compounds to Zn2+
ions was further confirmed in an NMR study, and the effects of common anions and change in pH were also
examined. The overall simplicity of our structure, alongside its unique properties and open possibilities for
structural modifications, makes it attractive for further research
Electrochemistry of different ruthenium polypyridine complexes
No full textIn search for antitumor metal-based drugs that would mitigate the severe side-effects of cisplatin, Ru(II) complexes
are gaining increasing recent interest. Their cytotoxic effect is widely known, however mechanism of
action, solution behavior, redox reactions within biological system are still focus of the new studies. Various
experiments and approach techniques are used to better understand ruthenium chemistry. In this order their
biological activity and the availability of reduction potential in the biological medium, it is necessary to know
their electrochemical redox behavior and properties. In this work, we report the electrochemical activity on
synthesized and characterized (1H- and 13C NMR, FT-IR, MS) half-sandwich organometallic Ru(II) complexes of
the general formula [Ru(η6-arene)(XY)Cl](PF6) where arene = benzene, toluene or p-cymene and XY = bidentates:
dipyrido[3,2-a:2′,3′-c]phenazine (dppz derivatives) or 2-(9-anthryl)-1H-imidazo[4,5-f][1,10]phenanthroline
(aip), which are bound to Ru(II) via two phenanthroline-N atoms in a characteristic “piano-stool”
configuration of Ru(II)-arene complexes – as confirmed by vibrational and NMR spectra. It is shown that selected
complexes can be divided in four groups, based on their electrochemical behavior. These behaviors are correlated
with their structure and nature of ligands
Research data no. 1: Critical Knowledge and Diagnostic Gaps for Shellfish Allergies: Insights from Global Market Presence and Trade of Shellfish
No full textTropomyosin sequences were compared between different shellfish species by performing BLAST protein sequence alignment. Comparisons were conducted exclusively on complete sequences, whether derived at the protein level, transcript level, or inferred through homology.Related to research data no. 2: [https://cherry.chem.bg.ac.rs/handle/123456789/7052
Određivanje antioksidativnog potencijala ekstrakata bobičastog voća dobijenih primenom prirodnih eutektičkih smeša
No full textEutektičke smeše (engl. Deep Eutectic Solvent - DES) i njihova podklasa prirodne
eutektičke smeše (engl. Natural Deep Eutectic Solvent - NADES), predstavljaju inovativnu
klasu rastvarača, koji kombinuju ekološke prednosti, nisku toksičnost i visoku
efikasnost u primeni. Njihova sposobnost da se koriste kao rastvarači za ekstrakciju
bioaktivnih jedinjenja otvara nove perspektive za primenu u analitičkoj hemiji,
biomedicini, farmacutskoj, prehrambenoj i kozmetičkoj industriji. Shodno tome u ovom
radu je urađena optimizacija prirodnih eutektičkih smeša kao rastvarača za ekstrakciju
liofilizovanog ploda sorte maline Tula Magic. Korišćena je UV-VIS spektrofotometrija
kao metoda određivanja ukupnih antiokisdanasa primenom DPPH testa. Ispitivanje je
urađeno u skladu sa principima zelene hemije. Dobijeni rezultati ukazuju da je
najpogodnija eutektička smeša korišćena kao rastvarač bila holin-hlorid sa glicerolom
i 30% vode (DES 3/5), koji je dalje korišćen za određivanje antioksidativnog potencijala
različitih liofilizovanih sorti kupina. Najveću antioksidativnu aktivnost uz primenu
eutektičke smeše DES 3/5 pokazala je sorta kupine Loch Ness na osnovu najmanje
vrednosti. Sadržaj vode u sortama kupina bio je u opsegu od 67,47 do 77,33%, dok je sadržaj
suvog ostatka od 27,09 do 32,65%
Sustainable water treatment: an advanced electrochemical approach for propranolol removal with a mechanistic study
No full textTo provide safe water resources, effective treatment of wastewater effluents is crucial. Its wide use and resistance to chemical degradation and biodegradation make propranolol (PRO) persistent in the environment. Advanced oxidation processes (AOP), such as electrochemical methods, offer promising solutions for removing organic pollutants from water, relying on the generation of reactive species. In this study, a PRO solution (30 mg/L, 125 mL) was electrochemically treated at pH 3 in the presence of Na₂SO₄ (4.26 g/L). The applied current density was 50 mA/cm², with Ti/Sb-SnO₂/PbO₂ as anode and Ti as cathode. This setup led to over 94% of PRO being removed within 90 minutes (Figure 1), as monitored by HPLC-DAD. Subsequently, LC-MS analysis revealed the structure of five degradation products (P134, P274-a, P274-b, P292-a, and P292-b). Furthermore, their ecotoxicity was assessed using USA EPA TEST 5.1.2. software and expressed as an acute toxicity (96 hours) LC₅₀ for fathead minnow (Pimephales promelas). P134 (1.9 mg/L) had lower toxicity compared to PRO (4.4 mg/L), while the rest of the compounds showed higher toxicity. Using the density functional theory (DFT) the most reactive sites in PRO were determined and Fukui indices enhanced the understanding of the fate of PRO within catalytic processes. This study demonstrated the effectiveness of an electrochemical approach for the removal of PRO from water, highlighting the importance of considering both degradation efficiency and the ecotoxicity of generated byproducts for sustainable water treatment.19th International Conference on Chemistry and the Environment (ICCE) 2025 was held in Belgrade, Serbia, from the 8th to 12th June 2025 at Hotel M, Belgrade, Serbia.More information on the conference here: [https://icce2025.com]This presentation was given by Slađana Savić on the 9th of June 2025 within the Session A2: Water chemistry, water pollution and water treatment.Presentation: [https://cherry.chem.bg.ac.rs/handle/123456789/7103
A rapid, reusable, and portable electrochemical assay for caffeine monitoring in beverage samples based on boron doped diamond and multi walled carbon nanotubes
No full textDespite potential health risks at high doses, caffeine remains the most widely consumed psychoactive drug globally, naturally occurring in more than 60 plants. Accurate determination of caffeine content is crucial to ensure the safety of consumers of caffeine-containing beverages. This work explores two different electrochemical sensors for caffeine determination: screen-printed carbon electrodes modified with multi-walled carbon nanotubes (MWCNT SPEs) and screen-printed sensors with boron-doped diamond electrodes prepared by chemical vapor deposition (BDD SPEs). These sensors offer advantages over traditional methods, potentially providing faster and more portable analysis. Two linear ranges for caffeine determination were observed at BDD SPEs in 0.5 M H2SO4. A lower linear range between 20 μM and 80 μM resulted in a limit of detection (LOD) of 3.40 μM and a limit of quantification (LOQ) of 10.30 μM, while a higher linear range between 100 μM to 500 μM provided the LOD of 9.72 μM and the LOQ of 29.45 μM of caffeine. MWCNT SPEs showed the optimal analytical parameters in the Britton-Robinson buffer at pH 2 with a broader linear range from 33 μM to 500 μM; the LOD was 8.65 μM, and the LOQ was 26.20 μM. The determination of caffeine content was successfully conducted in real dietary samples using both sensors, with validation by high-performance liquid chromatography (HPLC) and spectrophotometric analysis.Peer-reviewed manuscript: [https://cherry.chem.bg.ac.rs/handle/123456789/7106]Supplementary material: [https://cherry.chem.bg.ac.rs/handle/123456789/7107
Priprema i karakterizacija enzimskih hidrolizata brašna cvrčka Acheta domesticus
No full textPorast svetske populacije, zajedno sa promenama u načinu života, dovodi do uvođenja novih prehrambenih praksi, posebno u regionima u kojima pojedine vrste hrane nisu bile tradicionalno zastupljene. Jedan od sve aktuelnijih primera jeste rastuća popularnost insekata kao sastavnog dela svakodnevne ishrane. Uključivanje insekata u ljudsku ishranu postaje globalno značajno, ne samo zbog njihovog bogatog nutritivnog profila, već i zbog održivosti njihove proizvodnje. U mnogim delovima Afrike, Azije i Latinske Amerike, insekti su vekovima služili kao značajan izvor proteina, zdravih masti i esencijalnih mikronutrijenata, pri čemu su vrste poput skakavaca, gusenica, cvrčaka i termita redovno korišćene u lokalnoj kulinarskoj tradiciji. U poslednje vreme, insekti su privukli pažnju i u zapadnim zemljama, gde su prepoznati kao održiva alternativa konvencionalnim izvorima proteina. Njihova proizvodnja troši znatno manje resursa u odnosu na stočarsku industriju, što rezultira nižom emisijom gasova sa efektom staklene bašte i smanjenom potrošnjom vode. Ovakvi proizvodi, uključujući proteinske pločice, brašno od cvrčaka i druge prerađevine od insekata, sve češće se mogu naći u komercijalnoj ponudi, ukazujući na njihov značajan potencijal u rešavanju globalnih izazova vezanih za prehrambenu sigurnost.
U ovom završnom radu istraživana je priprema i karakterizacija enzimskih hidrolizata proteina iz komercijalno kupljenog brašna cvrčka vrste Acheta domesticus. Hidrolizati su pripremljeni primenom različitih enzima, a zatim i okarakterisani standardnim biohemijskim metodama, uključujući spektrofotometrijske (određivanje koncentracije proteina, peptida, antioksidativni eseji) i elektroforetske tehnike. Preliminarni rezultati ukazuju da kvalitet enzimskih hidrolizata značajno zavisi od odabranih uslova hidrolize, kao što su vrsta enzima, količina enzima i vreme trajanja digestije. Ovi hidrolizati pružaju važne informacije za potencijalne primene u prehrambenoj industriji i mogu poslužiti kao osnova za dalja istraživanja u oblasti nutritivnih svojstava insekata
Phytochemical Properties, Antioxidant Capacity, and Hypoglycemic Potential of Goji Berry Juice from Serbia
No full textGoji berry is a rich source of polyphenols, carotenoids and polysaccharides, contributing to its diverse biological activities. Due to their high water content and perishability, the berries are often processed into dried forms or juices. This study hypothesized that juice obtained from Serbian-grown goji berries would exhibit a distinctive phytochemical composition and significant in vitro antioxidant and hypoglycemic potential. Antioxidant activity was assessed using DPPH, ABTS, CUPRAC, FRAP and β-carotene bleaching assays, while hypoglycemic potential was evaluated via α-amylase and α-glucosidase inhibition. The analyzed goji juice exhibited mild acidity and moderate sweetness. In terms of bioactive composition, the juice contained high levels of polyphenols (194.50 ± 3.88 mg GAE/100 mL) exceeding the values reported for most commercial fruit juices, as well as notable levels of flavonoids (70.30 ± 5.11 mg CE/100 mL), carotenoids (289.53 ± 0.65 µg/100 mL) and polysaccharides (375.20 ± 12.46 mg Glu/100 mL), along with minerals, particularly potassium and copper. It also showed strong antioxidant capacity and concentration-dependent inhibition of α-amylase (IC50 = 5.28 ± 0.26 mg/mL) and α-glucosidase (IC50 = 10.12 ± 0.23 mg/mL). This study provides the first comprehensive characterization of Serbian-grown goji berry juice, confirming its potential as a functional ingredient