Journal of Applied and Natural Science
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Morphological variations in populations of Urginea indica Kunth.Liliaceae
Get PDFAn attempt has been made to enquire into the morphological variations which leads to evolutionary divergence of populations of Urginea indica Kunth. In particular, there were considerable morphological variations within the species. The thirty two cytotypes showed distinct morphological differences in shape, size and colour of bulb and leaf, the length of inflorescence and flower colour. The morphological complexity is accompanied by high degree of cytological variations. Preliminary measurements of reproductive, characters have shown that no noteworthyresults may be obtained in this characters except pedicel length but the vegetative character deviated significantly. Flowering and blooming time varied that also played a role in isolation of these populations. These morphological differences have a genetic basis and would be worthy in recognizing them as a separate sub specific taxon
Fish fauna of Surha Tal of District-Ballia (U.P.), India
Get PDFThe present study on survey of Surha Tal of district Ballia, U. P. for fish fauna showed the presence of 59 species belonging to 40 genera of 22 families and 8 orders
Composition variation in essential oils of Artemisia nilagirica and Artemisia capillaris, growing in India
Get PDFThe present study was aimed to find out the chemical constituents of essential oils of Artemisia nilagirica (Clarke) Pamp. and Artemisia capillaris Thunb. of Asteraceae family, growing in Garhwal region of India. The essential oils were obtained by hydro-distillation and subjected to detailed GC-MS analysis in order to determine the variation in their volatile constituents. While comparing the common constituents in both of the species, the remarkable variation was observed and it was 6.03, 3.251, 2.093, 33.73, 7.573, 15.041 and 8.00% for trans-caryophyllene, DL-limonene, ?-pinene, ?-thujone, ?-myrcene, ?-ocimene and ?-thujone, respectively
Study of chromosomal abnormalities in Channa punctatus exposed to fenvalerate
Get PDFThe chromosomal aberration test in kidney cells of Channa punctatus was conducted to study the genotoxic effect of synthetic pyrethroid fenvalerate, Different chromosomal abnormalities were seen and their frequency was recoded in metaphase spreads obtained from fishes exposed to three sub lethal concentrations (0.0625, 0.0314 and 0.0157 ppm) of this compound. The exposure to fenvalerate caused various structural abnormalities in chromosomes such as chromatid break, fragment, gap, chromatid separation, deletion and ring type chromosomes. Results obtained in present investigations clearly indicate that fenvalerate acts as a genotoxic agent in Channa punctatus
Diagnostic features of three nymphal instars of Sturnidoecus bannoo (Phthiraptera: Ischnocera) infesting Bank Myna Acridotheres ginginianus
Get PDFThree nymphal instars of bank Myna louse, Sturnidoecus bannoo differ from each other not only any size, abdominal segmentation, chitinization but also in the number of setae occurring on head, thorax and abdomen. Present report provides information on the diagnostic characters of three nymphal instars of the aforesaid louse
Significance of cyanobacterial diversity in different ecological conditions of Meghalaya, India
Get PDFThe present study deals with preliminary investigation of cyanobacterial diversity in Meghalaya. A total of 75 samples were collected from 10 different ecosystems and analyzed. 65 strains of cyanobacteria isolated under 11 genera include Nostoc, Anabaena, Calothrix, Cylindrospermum, Gleocapsa, Fischerella, Plectonema, Tolypothrix, Stigonema, Loriella and Westiellopsis. Nostoc was most abundant. Diversity analysis indicated maximum Shannon’s diversity index (H) in Mawlai. Highest Simpson’s diversity index was seen in Sung Valley (0.75). Both Shannon’s and Simpson’s diversity indices were lowest in Mairang. Richness was highest in Sung valley and Syntuksiar with both the sites supporting 17 strains each. Although, highest diversity was recorded from Mawlai, richness recorded at this site was only 11 strains thereby indicating richness need not be a function of diversity in this region. This study revealed the cyanobacterial strains, which can withstand acidic pH and prevail in the region. A study on colonization also identified some potential biofertilizer strains from the region such as Nostoc punctiforme, Nostoc muscurum and Anabaena azollae that could be effective in acidic crop fields
Effect of fly ash on surface tension of few liquids
Get PDFSurface tension of different liquids which have an impact on living systems and the changes occurred due to mixing of fly ash was studied, keeping the temperature and amount of fly ash powder, constant. This change is very vital because surface tension is a very vital element for survival in biological world as many animals, and microorganisms survive due to specific surface tension which supports their life on water as larva or adult and even human, because many vital processes like respiration is dependent on surface tension.When yeast cells in aqueoussolution, surface tension of 6.65 x10-2Newton/Meter at280 C respiration is best but at 5.1x10-2 Newton/meter respiration is inhibited (1953 Carl)
Isolation, purification, characterization and applications of serine protease from Bacillus megaterium
Get PDFBacillus megaterium isolated from poultry farm soil was identified by standard biochemical tests and screened for the production of serine protease. Production of serine protease was done using 5 different medias by varying the type of amino acid added. The purification was done by salt precipitation, dialysis and DEAEcellulose ion exchange chromatography. The proline containing media obtained the highest fold purification out of the five different medias (leucine, lysine, proline, tryptophan and methionine cotaining media). The enzyme showedan optimal activity at the temperature 37°C and the pH 6 which are known as its optimum temperature and pH respectively. The enzyme was proved as a Mn2+ dependent serine protease as it was activated by Mn2+ ions and inhibited by PMSF. The molecular weight of the enzyme was determined by SDS-PAGE technique as around 30kDa. It showed an excellent detergent activity on the blood stains and a very good stability in presence of locally available detrgents. The enzyme acted on the keratin protein of the chicken feather and showed a degrading capacity on the protein. So it was proved that the recently studied serine protease has a keratinase activity also. From these datas I conclude that the protease isolated from Bacillus megaterium is a Mn2+ dependent serine protease which has both keratinase and detergent activity
Assessment of bacteria and SPM in the indoor air of households of urban area of Jammu (J&K), India
Get PDFThe present study was conducted to assess status of bacteria (Gram +ve and Gram –ve) in the indoor air of Households located in Jammu city. The study area was divided into eight Sites. At each site two Households were selected randomly and in each Household sampling of SPM (?g/m3) and bacteria (CFU/m3) was done twice at three sub sites. Total bacterial count and SPM was found to be maximum (9308.24 CFU/m3and1006.12 ?g/m3 respectively) in Households near Water Body and total bacterial count and SPM was minimum (5251.00 CFU/m3and 659.09?g/m3respectively) in Households near Hospital. A significant positive correlation (r) was found between SPM and no. of Gram +ve (+0.18 to +0.78) as well as between SPM and no. of Gram –ve (+0.21 to +0.76) bacteria in the study area
Management of sheath blight in rice through application of Validamycin, Trichoderma harzianum and Pseudomonas fluorescence
Get PDFEleven different combinations of Trichoderma harzianum, Psedumonas fluorescens and Validamycin 3L, were applied at different crop stages viz. seed, seedling, tillering and symptoms initiation along with one application of Carbendazim as standard control to test their efficacy against sheath blight of rice caused by Rhizoctonia solani under field condition. One application of Carbendazim @ 0.1% at the time of symptoms initiation (with 81.36 percent reduction of disease incidence, 83.17 percent reduction in disease severity and 62.92 percent inhancement in yield) and seed treatment with Trichoderma harzianum and seedling root dipping with Pseudomonas fluorescens and two spraying of Validamycin i.e. at tillering and symptoms initiation stages (with 69.10 percent reduction of disease incidence,83.17 percent reduction in disease severity and 62.49 percent inhancement in yield) were found to be equally and highly effective in reducing the sheath blight severity and in increasing rice grain yield also. One spraying of Validamycin at symptoms initiation stage was comparatively less effective (29.54 per cent reduction in disease incidence,53.46 per cent reduction in disease severity and 47.76 per cent enhancement in yield ) than one spraying of Carbendazim at symptoms initiation stage