Journal of Applied and Natural Science
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Bio-efficacy of phytoextracts and oil cakes on Macrophomina phaseolina (Tassi) causing stem rot disease of jute, Corchorus spp.
Get PDFIn the present study efforts were made to explore the efficacy of various plant extracts and oil cakes against Macrophomina phaseolina. The efficacy of eight different plant species, Zingiber officianale, Aloe indica, Lawsonia inermis, Chenopodium album, Allium cepa, Piper betel, Murraya koenigii, Parthenium hysterophorus aqueous extracts including oil cakes of mustard and neem were evaluated by using of poisoned food technique in vitro for their inhibitory effect on mycelial growth of M. phaseolina causing stem rot of jute (Corchorus olitorius and C. capsularis). The rhizome extract of Z. officinale produced maximum growth inhibition (74.59%) of the pathogen followed by leaf extracts of A. indica (63.57%), and leaf extract of L. inermis (60.17%) at the concentration of 10%. The maximum mycelial growth inhibition (52.40%) was recorded with neem cake (Azadirachta indica) at the concentrations of 20% followed by 42.61% and 29.60% with concentration of 15% and 10%, respectively. However, maximum mycelial growth inhibition (19.42%) was recorded with mustard cake (Brassica juncea) at the concentration of 20% followed by 16.64% and 12.20% at the concentration of 15% and 10% respectively. In general mycelial growth inhibition was dose dependent and it was maximum in case of neem cake than mustard cake. The present study revealed that, these plant extracts and oilcake extracts could be exploited for the possible control of deadly pathogen M. phaseolina. Accordingly, this is an important proactive measure in preventing the spread of the stem rot disease through a more ecofriendly approach
A study on treatment of resistant mastitis in dairy cows
Get PDFThe study was undertaken to determine the prevalence and treatment of antibiotic resistant mastitis in dairy cows. The predominant resistant causative pathogen was Escherichia coli (50.64 %) followed by S. aureus (44.25 %) and Methicillin resistant Staphylococcal aureus (5.11%).These isolates were found sensitive to gentamicin, enrofloxcain, amoxicillin+sulbactam, ceftriaxone and resistant to amoxicillin, oxytetracycline, penicillin G and oxacillin. In all the treatment groups of E. coli, S. aureus and MRSA mastitis, the post treatment pH, SCC was significantly (P < 0.01) decreased when compared to pre treatment pH, SCC values and the post treatment electrical conductivity was significantly (P < 0.01) increased when compared to pre treatment electrical conductivity value. In E. coli mastitis, treated with amoxicillin+sulbactam, ceftriaxone, enrofloxacin and gentamicin showed 74.1%, 67.75 %, 76.67 % and 64.52 % clinical recovery and in S. aureus mastitis, showed 65.25 %, 65.25 %, 72.43 % and 68.98 % clinical recovery. In MRSA mastitis, enrofloxacin was found to be highly effective in comparison to amoxicillin+sulbcactam
Effect of DEM data resolution on low relief region sub-watershed boundaries delineating using of SWAT model and DEM derived from CARTOSAT-1 (IRS-P5), SRTM and ASTER
Get PDFWatersheds are natural integrators of hydrological, biological, and geological processes and as such require an integrated approach to data analysis and modeling, which usually starts delineating accurately a polygon vector layer of watershed boundaries as input. In that way, Garrah sub-watershed in Shahjahanpur district of U.P., India, had been isolated with the objective of evaluate the accuracy of sub-watershed boundaries derived from three different sources: One was delineated by 2.6 m resolution CARTOSAT-1 DEM (IRS-P5) and other two were derived from a 30 m ASTER DEM and a 90 m SRTM DEM, using the Soil and Water Assessment Tool (SWAT) model. In this analysis we found that the accuracy of CARTOSAT-1 DEM is so best for accurate delineation of a sub-watershed in the management of the watershed, and the coarse spatial resolution SRTM DEM (90 m) performed much better and significantly than the high spatial resolution ASTER DEM (30 m) it’s cause of the errors in the ASTER DEM, map algebra was used to define where the “Fill” tool had filled the sinks finding that the errors in the stream network occurred where some especially large fills had occurred. Not only the visual interpretation of the produces figures indicate the SRTM DEM delineated sub-watershed as relatively more accurate from ASTER DEM delineated sub-watershed boundaries, also statistics for the SRTM DEM elevation turned in significantly higher than that of the ASTER DEM. Overall SRTM DEMs offer more precise elevations while, ASTER DEMs offer more details. Therefore, the accuracy of the sub-watershed delineation depends on the first place on the accuracy of the DEM
Growth promotion of transplanted rice plant by bioagents effective against bacterial leaf blight disease of rice under glasshouse conditions
Get PDFThe experiment was conducted to evaluate growth promotion of transplanted rice by fungal and bacterial bioagents effective against bacterial leaf blight of rice, under glasshouse conditions. Bioagent formulations (PBA -1, PBA -2, FLP 88, Pf 83, Isolate 40 and T. harzianum) were applied as seed treatment, seedling root dip and foliar spray and compared with chemical treatment and untreated check. All bioagent formulations were significantly effective in increasing number of tillers per hill, root length, total phenolic content in rice leaves, fresh and dry root weight, fresh and dry shoot weight, flag leaf area, grain yield and 1000 grain weight over check. Present study revealed that potential fungal and bacterial bioagents effective against bacterial leaf blight disease of rice, exhibited plant growth promoting activities and resulted 22.38 to 32.08 % increase in grain yield over untreated check, even if there is no disease
Influence of operating conditions on the electrolytic treatment for the removal of color, TSS, hardness and alkalinity using Al-Al electrode combination
Get PDFThe present investigation observed the effect of current density (CD), operating time (OT), inter electrode distance (IED), electrode area (EA), initial pH and settling time (ST) using Al-Al electrode combination on the removal of color, total suspended solids (TSS), hardness (HR) and alkalinity (ALK) from biologically treated municipal wastewater (BTMW) of Sewage Treatment Plant (STP), Jagjeetpur, Haridwar, India. The maximum removal of color (99.86%), TSS (98.7%), HR (78.9%) and ALK (43.69 %) from BTMW was found with the optimum operating conditions of CD (2.65 A/m2), OT (40 mins.), IED (0.5 cm), EA (160 cm2), initial pH (7.5) and ST (60 min.). However, the maximum removal of ALK was found with the optimum operating conditions of CD (1.68 A/m2), OT (40 mins.), IED (1.0 cm), EA (80 cm2), initial pH (7.5) and ST (90 min.) The electrolytic treatment ( ET) brought down the concentration of HR and ALK to the desirable limit of the BIS standards of drinking water. There was no need of pH adjustment of the BTMW during ET as the optimal removal efficiency was close to the pH of 7.5. Under optimal operating conditions, the operating cost was found to be 1.01 $/m3 in terms of the electrode consumption (23.71x 10-5 kg Al/m3) and energy consumption (101.76 Kwh/m3). The study revealed that BTMW can be effectively treated with the Al-Al electrode combination for color, TSS, HR and ALK removal
Effect of integrated nutrient management on soil fertility and yield of maize crop (Zea mays) in Entic Haplustart in Tamil Nadu, India
Get PDFA field experiment was conducted to study the integrated use of organic and inorganic fertilizers on the soil fertility status of Entic Haplustart in maize at Malayalathanpatti village, situated at Madurai district during Kharif season of 2008-2009. The experiment was conducted in Randomized Block Design with three replications in Annaiyur soil series (Entic Haplustert). The results revealed that highest grain yield of 4402 kg ha-1 was recorded in treatment that received vermicompost @ 5t ha-1 with 75 percent Recommended dose of fertilizer (RDF) and it was found to be superior to over treatments. Application of vermicompost @ 5t ha-1+75 percent RDF recorded the highest calcium and magnesium content in pot harvest soil sample (0.139 and 0.281%). And also increased the crude protein (16.67 %) and starch (81.34 %) content in maize crop and it was followed by the treatment which received sewage sludge 2t ha-1 with 75 percent RDF (Recommended dose of fertilizers) and green leaf manure @12.5t ha-1whereas the lowest value was recorded in control plot respectively. The vermicompost along with inorganic fertilizers were efficiently used by maize crop for their growth and development and also maintainined soil fertility and increased yield of the crop
Phenology and thermal indices of maize (Zea mays L.) influenced by subsoil compaction and nitrogen fertilization under semi-arid irrigated conditions
Get PDFThe magnitude of yield reduction due to soil compaction is variable and depends on the soil type, fertility status and other soil and environmental factors. The present investigation was carried out at the research farm, Department of Soil Science, Punjab Agricultural University, Ludhiana. The experiment was conducted to evaluate the effect of different levels of subsoil compaction and nitrogen fertilization on maize phenology, yield and heat use efficiency. The C2 (subsoil bulk density (Db)= >1.8 Mg m-3) treatment reduced yield by 15.5 and 24.3 % and heat use efficiency (HUE) by 15.2 and 20.9 % than that in C0 (subsoil Db=1.55-1.65 Mg m-3) treatment during the year 2012 and 2013, respectively. The tasseling and silking stage was delayed, while physiological maturity was advanced under C2 subsoil compaction treatment than that in C0 treatment. The N2 treatment improved the yield by 14.9 and 13.9 % and HUE by 15.2 and 14.3 % than that in N0 treatment during the year 2012 and 2013, respectively. Maize took more days to reach physiological maturity under N2 treatment as compared to N0 treatment. Phenothermal index (PTI) showed that crop reached different stages earlier under C1 and C2 than that of C0. The data emphasized the need to take care of soil strength and soil temperature related parameters along with weather conditions for better yield prediction using thermal time
Optimization of fermentation conditions for producing Indian rock bee (Apis dorsata) mead using response surface methodology
Get PDFMead is a traditional drink which results from the alcoholic fermentation of diluted honey carried out by yeast (Saccharomyces cerevisiae KF233529). The present investigation was carried out for the optimization of fermentation parameters for maximizing the yield of ethanol. Response Surface Methodology (RSM) based central composite design was employed to obtain best combination of temperature, fermentation time and total soluble solids (TSS). The optimum conditions for ethanol yield were temperature 28°C, TSS 15°Brix and 6 days after fermentation. The model showed that the value of R2 (0.9998) was high and p- value of interaction of variance was <0.0001. Hence the model can be said to be of highly significant
Impact of abiotic factors on build-up of citrus psylla, Diaphorina citri Kuwayama population in Punjab, India
Get PDFSeasonal abundance of citrus psylla (Diaphorina citri) Kuwayama was studied on Kinnow mandarin under Punjab conditions during 2012 and 2013. Population of D. citri was present throughout the year but only adults found surviving during December and February. There was no nymphal population when maximum temperature was > 39°C or < 7°C. Two population peaks of nymphs wer e observed, first in April-May and second in August- September. Thereafter, there was abrupt decline in nymphal population during June and July during both the years when the weather conditions i.e. maximum, minimum temperature (°C), relative humidity (%), rainfall (m m) and sunshine (hrs) ranged from 24.4-44, 22.4-31.8, 25.5-100, 0-108.0 and 0-13.5, respectively. Highest peaks of adult were found during May followed by June. With the decrease in temperature from the end of September, the population of D. citri started to decrease and reached its lowest during winter season. Correlation analysis for both the years indicated that maximum and minimum temperature, sunshine and rainfall were positively correlated with nymphal, adult and mixed population but the effect of rainfall on adult population was non-significant, while relative humidity was negatively correlated. The study is useful to find out the weak links of psyllid populations in relation to abiotic factors and that could be exploited to curb its infestation and disease transmission
Specific identification, biology and symptoms of whitefly species infesting sunflower in South India
Get PDFWhitefly species related to sunflower was identified as Bemisia tabaci (Gennadius). Further the identified whitefly species was confirmed to be indigenous B. tabaci on molecular basis by using B-biotype specific SCARs and biological silver leaf assay on sensitive pumpkin (cv Big variety). None of the whitefly samples could positive for the presence of B biotype. The results of the study on the pest life cycle under the laboratory conditions showed that, B. tabaci passed through four nymphal instars before the adult stage. The mean duration values of these stages were 5.6, 4.2, 4.4 and 5.6 days respectively. The total duration of the life cycle of B. tabaci ranged from 23- 42 days at the temperature of 29±2°C with a mean of 34.5. The damage to sunflower crop caused by the whitefly species is discussed with a special emphasis on its ability to transmit leaf curl viral disease