Journal of Applied and Natural Science
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Effect of fungal and bacterial bioagent application on total phenolic content in rice leaves pre-inoculated with Xanthomonas oryzae pv. oryzae (Uyeda and Ishiyama) Dowson
Get PDFPresent study was carried out to observe the effect of fungal and bacterial bioagents on total phenolic content in rice leaves pre-inoculated with Xanthomonas oryzae pv. oryzae and on disease severity of bacterial leaf blight of rice. Two commercial formulations of Trichoderma harzianum (PBA-1) and Pseudomonas fluorescens (PBA-2) and four formulations of fluorescent pseudomonads and Trichoderma spp. viz, P. fluorescens (Pf 83, rice leaf isolate), fluorescent pseudomonad (FLP 88, rice leaf isolate), T. harzianum (rice leaf isolate), Trichoderma spp. (isolate 40, isolated from rice field soil) were evaluated. Significantly higher mean value of total phenolic content of rice leaves was observed with the application of bioagent formulations as compared to check (pre-inoculated with X. oryzae pv. oryzae), chemical treatment and healthy plant. Maximum mean total phenolic content (342.22 ?l/g) in rice leaves was observed with Pf 83, which was followed by PBA-2 (334.44 ?l/g) and T. harzianum (330.00 ?l/g). Decrease in disease severity of bacterial leaf blight was observed with the increase of total phenolic content in rice leaves which resulted in increased grain yield and 1000 grain weight
Dimensional stability and strength properties of wood plastic composites produced from sawdust of Cordia alliodora (Ruiz and Pav.)
Get PDFThis study evaluates the effects of densities and mixing ratio on the physical and mechanical properties of wood plastic composites boards at mixing ratio of 1:1 to 1:1.4 and nominal densities of 700kg/mm3 and 800kg/mm3. The quantity of High Density Polyethylene (HDPE) and saw dust used in the production of Wood Plastic Composites (WPCs) was weighed to a nominal density of 700kg/mm3 and 800kg/mm3. The materials were thoroughly mixed and fed into a neatly primed oil mould with a dimension of 300 x 300 x 10 mm. Test samples used for physical and mechanical properties determination were collected from each board produced from the mould. The results showed that WPCs board produced from mixing ratio 1:1 had the highest Modulus of Rupture (MOR) and Modulus of Elasticity (MOE) values of 6.52 mm N-2 and 564.95 mm N-2 respectively. Water absorption, thickness swelling and linear expansion of WPCs produced from wood/plastic ratio of 1:1.4 had the lowest mean values of 6.67, 0.83, 0.68% and 21.61, 1.33, 5.35% respectively after 2 hours and 24 hours of water immersion test. Analysis of variance carried out at 0.05% probability level showed that the effect of density and mixing ratio were significant on the physical and mechanical properties of wood plastic composites boards
Differential response of phosphorus utilization efficiency in rice by tracer technique using phosphorus-32 under phosphorus stress environment
Get PDFIn most soils, soil and fertilizer Phosphorus (P) are easily bound by either soil organic matter or chemicals and thus are unavailable to plants unless hydrolyzed to release inorganic phosphate. Therefore, the development of P-efficient rice varieties that can grow and yield better with low P supply is a key to improve crop production. P efficient plants play a major role in increasing crop yields due to shortage of inorganic P fertilizer resources, limited land and water resources and increasing environmental concerns. Based on the P uptake efficiency, four rice genotypes viz.,TNRH 180, CB08504, CB06732 and ADT 47 were selected from the field experiment and used in pot culture experiment with three levels of P using radio isotope technique to quantify the P acquisition efficiency (PAE) and P use efficiency (PUE) and also to determine the native P supplying power of the soils using 32P in low P soils. Growth and yield parameters, grain and straw yield and major nutrients uptake of rice genotypes were increased with enhanced level of phosphorus application. Among the four genotypes, TNRH 180 recorded the highest grain yield and uptake. Increasing the P application rate from 25 to 50 kg P2O5 ha-1 increased the %Pdff in grain and straw for all the genotypes. The mean per cent phosphorus utilization (PPU) ranged between 18.74 and 23.72. The PPU of the genotypes followed the order TNRH 180 (23.72 %) > CB08504 (23.36 %) > CB06732 (20.54%) > ADT 47 (18.74%). The PPU values were higher at lower level of P application (25 kg P2O5 ha-1) for the genotypes TNRH 180, CB08504 and CB06732. From this study showed that rice genotypes have the ability to utilize the both available and unavailable form of phosphorus by secreting some organic acids in the root portion to solubilize. Hence rice genotypes indicated above have the ability to increase phosphorus utilization efficiency
Growth physiology of Brassica rapa var. yellow sarson under integrated nutrient management and seed soaking approaches in eastern sub-Himalayan plains
Get PDFThe field experiment was conducted at Uttar Banga Krishi Viswavidyalaya, West Bengal, India during rabi 2007-08 to 2008-09 to study the growth and productivity of yellow sarson under different nutrient managementi practices and pre-sowing seed soaking. The highest value of LAI (4.00), apart from the maximum plant height (129.97cm), dry matter accumulation (481.93g m-2) at 90 days, crop growth rate (12.29 g m-2 day-1) in between 45 to 60 days, root dry weight (1.645 g plant-1) with the highest average root diameter (0.932 mm) were observed in treatments receiving 75% recommended dose of chemical fertilizer with farm yard manure, Azotobacter and phosphate solubilizing bacteria as non-chemical source. Similarly at 45 days of crop age, significantly higher stomatal conductance (701.68 m mol m-2 s-1) and transpiration rate (4.55 m mol m-2 s-1) were reflected by the same treatment combination. These were attributed to the production of maximum seed yield (1374 kg ha-1), which was 39.91% higher than the recommended dose of chemical fertilizer application. On an average, seeds soaked with water before sowing reflected 9% lesser yield (1103 kg ha-1) against chemical soaking. Yellow sarson crop grown with integrated nutrient management practice consisting 75% of the recommended dose of chemical fertilizer along with farm yard manure (5t ha-1), Azotobacter (5kg ha-1) and phosphate solubilizing bacteria (5kg ha-1) coupled with seed soaking in 100ppm KH2PO4 confirms to be the best treatment combination from the treatment schedule considered during the study for the sub-Himalayan plains of West Bengal, India in terms of crop growth and productivity
Induction of water stress tolerance of mustard plants using Trichoderma as biological seed treatment
Get PDFWater scarcity is one of the main consequences of changing climate which adversely affects the plant growth and productivity. Enhanced root development results in increased surface area of active absorption for water and nutrient uptake which helps in tolerating abiotic stresses including drought in plants. Trichoderma is well known for its biocontrol and growth promontory effect in plants in addition to alleviate abiotic stress. In our study, thirty isolates of Trichoderma were grown on sterilized cow dung at different moisture content ranges from 5 to 30 percent to investigate their ability to grow and multiply under water stress condition. Mustard plants were grown under glass house condition by treating seeds with selected isolates of Trichoderma subjected to water stress subsequently. All isolates of Trichoderma grew upto 20% moisture whereas only eleven isolates exhibited growth at 10% moisture. Isolate PB23 was only isolate which was able to grow and resulted in 1.0 x109 cfu/g air dried cow dung even at 5% moisture content and induced the tolerance of mustard plants under water stress conditions when applied as seed treatment before sowing
Combining ability and heterosis for yield, its component traits and some grain quality parameters in rice (Oryza sativa L.)
Get PDFThe experiment was conducted to estimate combining ability and heterosis for yield, yield attributing traits and few grain quality parameters in rice. The crosses were made among three CMS lines i.e., IR58025A, Pusa6A and IR68897A and seven pollen parents viz., Sarjoo-52, Jaya, Sasyashree, Swetha, HUR 5-2, PR-106 and BPT 5204. Twenty one hybrids were generated in line x tester design. The superior hybrids were identified on the basis of combining ability effect and heterosis. Cross combination IR68897A/Jaya and IR68897A/BPT 5204 exhibited good x good parental GCA effects suggesting that there is additive x additive type of gene action. The cross IR68897A/Jaya showed highest positive SCA effect. The higher magnitude of heterosis for all the yield and quality traits were not expressed in a single hybrid combination. It varied from cross to cross due to diverse genetic background of their parents. The two crosses IR68897A/Jaya and IR68897A/BPT 5204 were found to be heterotic for yield and yield traits as well as the grain quality characteristcs
Impact of different cropping systems on properties of soil and water in different micro watersheds
Get PDFStudies on soils of Navsari Agricultural University (NAU) having different cropping system were carried out in the four micro watersheds that exist in the 400 ha University campus. Soil samples from 0-15 cm depth and water samples from adjacent bore wells were collected and analyzed from different locations of micro watersheds. Soils of watersheds showed that soils texture was clay in nature, having more than 65 % clay, whereas silt was more in watershed ‘A’. Soil organic carbon content (SOC) was 0.32 %, found in the field near University play ground and the highest 0.88 %, in Forestry farm, thus underlining the need of forest species in agricultural farms. SOC levels have reduced significantly due to intensive cultivation in all the watersheds. The result of exchangeable sodium percent (ESP) is supported by the topographic features, as, ESP was more in watershed ‘B’ (5.15) than C (2.95), this showed that infiltration rate was lesser in ‘B’ as compared to ‘C’ due to availability of more sodium (Na). Available N was highest in watershed ‘A’ (246 kg/ha) followed by ‘C’ (225 kg/ha) than ‘B’ (203 kg/ha), the reason was watershed ‘A’ had only horticulture crops whereas B and C had different crops of the region. Electrical conductivity (EC) of ground water collected from wells in watershed ‘C’ was found to be very high both before (3.44 dS/m) and after monsoon (2.95 dS/m), showing that water is highly saline and not fit for surface irrigation and there is need of ground water recharging
Eco-friendly management of major diseases in jute (Corchorus olitorius L.)
Get PDFField experiment was conducted for disease management with seven eco-friendly treatments in Corchorus olitorius jute during 2012-2013 under randomized block design (RBD) in three replications with variety JRO-524. The ecofriendly treatment 50% N: P: K + seed treatments with Azotobacter and phosphorus solubilizing bacteria (PSB) @ 5g/Kg+ Trichoderma viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + Psuedomonas fluorescens spray @ 0.2% at 45DAS was found superior with minimum per cent disease incidence (3.46 and 1.43%) as compared to control (13.17 and 4.96%). The second best treatment was found with Farm yard manure (FYM) @ 5t/ha + seed treatment with Azotobecter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha 21DAS)+ P. fluorescencs spray @ 0.2% at 45DAS and showed 3.61 and 2.0% disease incidence. The treatment with N: P: K @ 60:30:30 and seed treatment with carbendazim 50WP @ 2g/Kg at 45DAS showed 4.56 and 2.10 % disease incidence. Dry fibre yield was highest (30.33q/ha) in the plots treated with FYM @ 5t/ha + seed treatment with Azotobacter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + P. fluorescencs spray @ 0.2% at 45DAS) and it was lowest in untreated control (15.69 q/ha).Variation in yield was attributed due to difference in plant height
Effect of different modes of pollination on quantitative and qualitative parameters of Egyptian Clover, Trifolium alexandrinum L.
Get PDFThe effect of different modes of pollination on quantitative and qualitative parameters of Egyptian clover, Trifolium alexandrinum L. was studied at Forage Section, Department of Genetics and Plant Breeding, CCS, Haryana Agricultural University, Hisar during 2012 and 2013. Maximum seed setting (81.5%) was recorded in A. mellifera pollination with an 8 frame colony (BP-8F) followed by 4 frame colony (BP-4F) (75.1%), open pollination (OP) (73.8%) and 2 frame colony (BP-2F) (71.5%). Maximum seed yield (2662.3 seeds) was observed in treatment BP-8F followed by BP-4F (2373.8), OP (2316.3) and BP-2F (2235.5). Still lower yield of 2103.0 seeds was found in hand pollination (HP) treatment that was significantly higher than the without insect pollination (WIP) treatment (1114.2). Minimum 1000-seed weight was observed in WIP (2.64 g). The seed weight of BP-4F (3.30), HP (3.20), BP-2F (3.17) and OP (3.03), the heaviest seeds were recorded in BP-8F (3.62 g/1000 grains) and it was at par with the treatment BP-4F (3.30 g). Highest seed germination per cent was recorded in BP-8F (94.7) followed by OP (90.7%). Lowest germination was found in WIP (84.7%). Though some work has been done on this aspect in India but comprehensive pollination studies has not been worked out
A review on weeds as source of novel plant growth promoting microbes for crop improvement
Get PDFIn the context of increasing international concern for food security and environmental quality, the use of bioinoculants like diazotrophs and plant growth-promoting rhizobacteria (PGPR) for reducing chemical inputs in agriculture is a potentially important issue. The improvement in agricultural sustainability requires optimal use and management of soil fertility and soil physical properties, where both rely on soil biological processes and soil biodiversity. Biological nitrogen fixation by plant-associated bacteria is eco-friendly and has been effectively exploited for crop plants including legumes. Although associations of rhizobacteria with non-leguminous plants such as grasses have been known for decades, they have been poorly - studied. Weedy grass species normally thrive in adverse conditions and act as potential habitats for the diverse groups of elite bacteria with multiple beneficial characters remains unexplored. A more complete understanding of the diversity and functioning of rhizobacterial microorganisms, especially those that have symbiotic relationships with grass species is of great value for agricultural research and application