Journal of Applied and Natural Science
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Influence of crop geometry on yield, yield attributes and glycoside yield of Stevia rebaudiana Bertoni
Get PDFField experiments were conducted in a loamy sand soil during 2006 and 2007 at Punjab Agriculture University, Ludhiana to evaluate the performance of Stevia rebaudiana Bertoni under varying planting geometry. The studies revealed that in case of row to row spacing, highest number of leaves per plant (533.0, 447.6), leaf area (8113.1, 6322.6 cm2/plant), leaf area index (5.1, 8.7) and dry matter accumulation per plant (86.0, 76.9 g/plant) during 2006 and 2007, respectively were found under wider row spacing of 75 cm. Which was significantly higher than narrower row spacing of 60 cm and 45 cm. Whereas, the fresh biomass yield (9861 and 11801 kg/ha), dry biomass (2080 and 2550 kg/ha), leaf yield (6129 and 4414 kg/ha) and stem yields (7611 and 5447 kg/ha) during 2006 and 2007, respectively and glycoside yield were registered higher under closer row spacing of 45 cm than the wider row spacing of 60 cm and 75 cm. In plant to plant spacings, the maximum number of leaves (5681.3 cm2/plant) was recorded under plant spacing of 45 cm which was statistically at par with plants spaced at 37.5 cm and 30.0 cm but significantly higher than 22.5 cm and 15.0 cm plant spacing, leaf area and dry matter accumulation per plant were highest with than other closer plant spacings.Whereas, the fresh and dry biomass, leaf and stem yields and glycoside yield were recorded highest under closer plant spacing of 15 cm which was statistically at par with 22.5 cm plant spacing
Study on yield potentiality and spatial requirement of rice varieties (Oryza sativa L.) in system of rice intensification (SRI) under red and laterite zone of West Bengal, India
Get PDFField experiment was conducted at Rice Research Station, Bankura during kharif season 2009 and 2010 to study the yield potentiality and spatial requirement of rice varieties in system of rice intensification (SRI) under red and laterite zone of West Bengal. The experiment was laid out in a randomized complete block design (RCBD) in a three replications with two rice varieties (Swarna and Lalat). Performances of swarna and lalat varieties in SRI as compared to conventional method of rice cultivation (CMRC) were investigated. Swarna (MTU 7029) has yielded maximum grain yield (6.07, 5.66 and 5.86 t ha-1 during 2009, 2010 and in pooled, respectively) from the treatment T7 (25 × 25 cm spacing) under SRI. Lowest grain yield (3.55, 3.23 and 3.38 t ha-1 during 2009, 2010 and in pooled, respectively) was recorded from treatment T9 (Lalat at 20 × 15 cm spacing) under CMRC. SRI technology has potential in increasing more grain yield, it saves seed requirement and irrigation water and chemical fertilizer considering than conventional method of cultivation. Rice cultivation is more sustainable and profitable for the farmers in SRI under the red and laterite zone of West Bengal
Impact of Piriformospora indica, Pseudomonas species and Mesorhizobium cicer on growth of chickpea (Cicer arietinum L.)
Get PDFThe present study was conducted to examine synergistic interactions among Piriformospora indica (PI) with potential plant growth promoting rhizobacteria (PGPR) and Mesorhizobium cicer (LGR33, MR) in two chickpea (Cicer arietinum L.) varieties viz. desi PBG1 and kabuli BG1053. Different PGPR species were used viz. native isolates of Pseudomonas argentinensis (LPGPR1), Pseudomonas sp. (LPGPR2) along with national check Pseudomonas sp. (LK884). Compatibility of MR, PI and differentPseudomonas spp. was studied by streak assay method and growth of fungal pellicle in-vitro. Consortium of MR+PI+LPGPR1 (0.605 g dry weight fungal pellicle/100ml nutrient broth) was found as the best compatible treatment. In vivo the synergistic effect of consortia was studied for improving dry weight of roots, nutrient acquisition, colonization and stress tolerance ability in chickpea. Significant improvement in dry weight of root was observed with MR+PI+LPGPR1 (1.316 g plant-1) in comparison to MR alone treatment (0.980 g plant-1) at 90 days after sowing (DAS). Percentage colonization of P.indica improved significantly with consortium MR+PI+LPGPR1 (75.5 and 78.3 %) treatment at 90 DAS. All the treatments significantly improved total soluble sugar content (12.2-26.9 %); amino N content (1.36-1.80 fold) and stress tolerance ability (4-6 fold) over the MR alone treatment. Reducing sugar content significantly improved with MR+PI+LPGPR1 (0.62 and 0.79 mg mL–1) over MR alone (0.42 and 0.58 mg mL–1) treatment in desiPBG1 and kabuli BG1053 chickpea, respectively. The tripartite combination MR+LPGPR1+PI can be explored as potent biofertilizer for improvment in chickpea productivity
Pretreated rice straw as an improved fodder for ruminants-An overview
Get PDFRice straw, a by-product of the rice production is mainly used as a source of feed for ruminant livestock is the major forage in rice-producing areas in India. The disposal of the rice straw is a serious problem in areas where it is the major agricultural product. It is rich in polysaccharides and has a high lignin and silica content, limiting voluntary intake and reducing degradability by rumen microbes. By rice straw treatment, its quality and digestibility can be improved and enhanced the protein content. Several methods have been used to improve the utilization of rice straw by ruminants or supplemented by other ingredients to increase digestibility and nutrient value before it can be considered a suitable animal feed. In recent years, biological treatments have been investigated for improvement in nutritional value of rice straw. The use of ligninolytic fungi and their extracellular ligninolytic enzymes for treatment of rice straw results in degrading cellulose and hemicelluloses contents which improve its nutritional value. The use of fungi and enzyme treatments is expected to be a practical, cost-effective and environmental-friendly approach for enhancing the nutritive value and digestibility of rice straw. Therefore, the treated rice straw has a good potential as feed for ruminants
Genetic variation and heritability estimation in Jatropha curcas L. progenies for seed yield and vegetative traits
Get PDFIn this study, experiment was conducted on sandy loam soils poor in organic carbon and water holding capacity in southern Haryana, India to determine the best progenies of Jatropha curcas for bio-diesel production. Fifty progenies raised from seed sources collected from nine states of India were evaluated after five years of plantation for growth, seed and oil content traits. The progenies showed significant (P> 0.05) differences for all the traits studied. Maximum seed yield/plant (879.37 g), number of capsules/plant (522.67) and plant height (408.33 cm) was recorded in P-44. Maximum oil content observed in P-37 (36.08%) followed by P-5 (35.64%). The magnitude of phenotypic coefficient of variation (PCV) was higher than the corresponding genotypic coefficient of variation (GCV) for all the characters studied. Heritability was highest for oil content (95.49%) and 100-seed weight (87.75%) followed by seed yield (75.54%). Total capsules/plant exhibited highest genetic advance (92.69%) followed by number of branches per plant (64.32%). Hierarchical Euclidean cluster analysis of all 50 progenies using D2 statistics was done where the D2 analysis grouped the progenies into five clusters. The intra cluster distances ranged from 1.33 to 2.72. The maximum inter-cluster distance was observed between cluster II and V (6.43) followed by I and V indicating greater divergence among progenies belonging to these clusters and an attempt to cross the progenies in these clusters should bring out desirable gene combinations. These progenies showed considerable potential which can be tapped for planting and selecting the improved varieties
Effect of feed supplemented exogenous bacteria, Lactobacillus sporogenes on the growth and body composition of Cirrhinus mrigala fingerlings
Get PDFProbiotics are known to improve the growth rate of aquatic organisms. Little experimentation has focused on supplementation of live colony forming units ofL.sporogenes in Cirrhinus mrigala to access the growth performance. A feeding trial was conducted for 50 days to study the effects of Lactobacillus sporogenes on growth and body composition of Cirrhinus mrigala (Hamilton-Buchnan, 1822) fingerlings. Fishes were fed with five experimental feeds containing 3.31x105 colony forming units (CFU)/100g-1 of L. sporogenes in diet D2 , 1.65×106 CFU/ 100g-1 in diet D3, 3.31×106 CFU/100g-1 in diet D4 and 4.96×106 CFU/100g-1 in diet D5. Control diet D1 was kept devoid of L. sporogenes. The diets prepared were containing 0.0% (D1), 0.1% (D2), 0.5% (D3), 1.0% (D4) and 1.5%(D5) of live cells of L. sporogenes CFU/100gm-1 of feed. Increasing trend of weight gain, specific growth rate (SGR), protein efficiency ratio (PER) improved food conversion ratio (FCR) was observed in treatments fed with probiotic added feeds than control feed. Best growth performance was found in the treatment fed with D4 of L. sporogenes containing 3.31×106 CFU 100g-1 of feed. At increased level 4.96×106 CFU 100g-1 of L. sporogenes inclusion the growth performance was dropped. Tissue protein content was also observed to be higher in animals fed with D4 feed. The lipid content was significantly (P<0.05) higher in fingerlings fed with control feed (D1). In short, L. sporogenes addition to a level of 3.31×106 CFU 100g-1 in feed improved growth performance of C. mrigala fingerlings. Therefore, it is evident that 3.31×106 CFU 100g-1 of feed of L. sporogenes can be considered as suitable concentration for attaining good growth in C.mrigala fingerlings
Effect of the size of cuttings (length and diameter) on rooting in cuttings of apple clonal rootstock Merton 793
Get PDFThe study was conducted in net house conditions of fruit Nursery block of Department of Fruit Science, Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan H.P. during 2012-2013 in order to evaluate the effect of size of cuttings (length and diameter) on rooting in cuttings of apple clonal rootstock Merton 793. Cuttings of length (L) viz. 15 cm, 25 cm, 35 cm, 45 cm and diameter ranges (D) viz. 0.75 cm – 1.00 cm, 1.00 cm – 1.25 cm, 1.25 – 1.50 cm were taken. The experiment was laid out in randomized block design (factorial). The cuttings were treated with 2500 ppm IBA (Indole – 3 – butyric acid) as quick dip (10 – 15 seconds) before planting. The results revealed that the cuttings with treatment combination of length (35 cm) and diameter (1.25 cm – 1.50 cm) recorded the highest rooting of cuttings (65 %), number of primary roots (5.00), length of primary roots (28.43 cm), diameter of primary roots (3.25 mm), fresh weight of roots (3.67 g) and dry weight of roots (2.59 g). Hence it is concluded that cuttings of length of 35 cm and diameter of 1.25 cm to 1.50 cm was most suitable for propagation. However, the cuttings of shorter length and diameter, are also able to result in rooting with lower success, therefore, these cuttings would be helpful in case of scarce availability of propagating material
Effect of plant and animal protein sources on the growth, gonadal maturity and proximate composition of Labeo rohita (Hamilton, 1822)
Get PDFThe aim of the present study was to evaluate the effect of plant and animal sources in the diet of Labeo rohita with an overall goal of gaining sustainable fish and egg production. Fishes with an initial weight of 310-323 g were fed with five different isocaloric (3Kcalg-1) diets viz. D1 ( control diet- 30% rice bran + 70% groundnut meal), D2 (30% rice bran + 50% groundnut meal + 20% fish meal), D3 (30% rice bran + 50% groundnut meal + 20% mustard meal), D4 (30% rice bran + 30% groundnut meal + 20% mustard meal + 20 % fish meal) and D5 (30% rice bran + 30% soybean meal + 20% mustard meal + 20 % fish meal) @ 3% of fish biomass for 270 days. Significantly higher weight gain and better gonadal maturity was recorded in fishes fed with diet containing fish meal than other. Among diets containing fish meal (D2, D4, D5), fish fed on diet D2 resulted in higher somatic growth (35.67, 42.80, 28.10 and 18.48% higher net weight gain than D1, D3, D4 and D5, respectively) and better gonadal development (43.20, 50.08, 22.59 and 23.25% higher absolute fecundity than D1, D3, D4 and D5,respectively) in L. rohita. Hence, Our study revealed that for higher growth and better broodstock development, L. rohita may be fed on diet formulated with 30% rice bran, 50% groundnut meal and 20% fish meal
Effect of body cooling systems on micro-climatic variable in semi -loose house for animals during hot-dry and hot-humid conditions of costal india
Get PDFTo study the effect of cooling system on microclimate variable three treatments based on animal body cooling systems i.e., shelter without cooling system (control, T1),with fogging (T2) and with showering (T3) in semi-loose house were designed. Common environmental variables like maximum and minimum temperature and relative humidity were recorded during hot-dry and hot-humid conditions. The maximum temperature (oC) was found significantly (P< 0.05) lower during hot-dry condition under fogging system (T2- 32.28 ± 0.23) than other treatments (T1- 33.89 ± 0.29 and T3-33.17 ± 0.26). Moreover, during hot-humid condition showering (T3-31.09 ± 0.16) was also significantly (P< 0.05) effective. Overall average maximum microclimatic temperature (oC) in T1, T2, and T3 was lower as compared to open macroclimatic. Relative humidity (%) was significantly (P< 0.05) higher in hot-humid as compared to hot-dry condition in respective treatments, (T1-79.09 ± 1.09 Vs 65.53 ± 1.00, T2-85.10 ± 0.86 Vs. 76.84 ± 0.73, T3-80.58 ± 1.05Vs. 67.83 ± 0.95 and open 79.94 ± 1.12 Vs 55.64 ± 1.07). During afternoon (2:30 PM) the per cent THI was found significantly (P< 0.05) lower under fogging (T2-80.22 ± 0.20) and showering (T3- 80.38 ± 0.21) as compared to control (T1-82.43 ± 0.21) during hot-dry condition. Overall result of treatments showed that the afternoon percent THI was significantly (P< 0.05) lower under showering (T3-80.65 ± 0.17) than other treatments (T1-83.31 ± 0.17 and T2-81.94 ± 0.15) and it was significantly (P< 0.05) different within cooling treatments like T2 and T3. This study showed significant effect of cooling system. Moreover fogging was better as it utilized less water, as compared to showering during hot dry condition
Genetic analysis of yield and heat stress related traits in wheat (Triticum aestivum L. em. Thell) using microsatellite markers
Get PDFMicrosatellite markers were used for genetic analysis of terminal heat tolerance in F2 (PBW373 × WH1081) population of wheat (Triticum aestivum L. em. Thell). Two parents were evaluated in field under normal sown and late sown conditions. For genotyping DNA from both parents PBW373 and WH1081 was amplified using 200 SSRs. Only 22 SSRs produced polymorphic bands, of size between 100 to 300 bp and an average of 1.45 alleles. The single marker analysis identified 19 markers indicating the putative QTLs for yield, its components and heat stress related physiological traits. The number of markers on these 16 linkage groups varied from one to four. On A genome 13 QTLs on B genome 5 QTLs and on D genome 9 QTLs were identified, respectively. The A, B and D genomes had 1360.3 cM, 272.4 cM and 919.5 cM of linkage coverage with average interval distances of 104.63 cM, 54.48 cM and 102.16 cM/Marker. A total of nine QTLs were resolved following composite interval mapping, one QTL was detected at a LOD score equal to threshold value of 2.5 while eight at LOD scores above the threshold value. All the nine QTLs were shown to be on definitive location on chromosome 3A (QDh.CCSHAU-3A, QDa.CCSHAU-3A and QPm.CCSHAU-3A), chromosome (QBm.CCSHAU-5A, QCtd.CCSHAU-5A and QCl.fl.CCSHAU-5A), chromosome6A (QPh.CCSHAU-6A) and chromosome3B (QTgw.CCSHAU and QMts.CCSHAU-3B). Use of these markers save times, resources and energy that are needed not only for raising large segregating populations for sveral generations, but also for estimating the parameters used for selection