Journal of Applied and Natural Science
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    Combining ability and heterosis for grain yield, fodder yield and other agronomic traits in sorghum [Sorghum bicolor (L.) Moench]

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    Forty five hybrids derived from a diallel mating design of ten parents and one standard check PUSA CHARI-121 were evaluated for general and specific combining ability effects and standard heterosis for grain yield/plant, dry fodder yield/plant, days to 50 per cent flowering, plant height, number of leaves/plant, leaf length, leaf breadth and 100- seed weight of Sorghum bicolor. The mean square due to general and specific combining ability was significan for Days to 50% flowering, Days to maturity, Plant height (cm),No of Leaves/plant, Leaf length (cm), Leaf width (cm), seed yield/plant (g), Fodder yield/plant (q/ha) and 100-seed weight (g) for all the characters. Both additive and non additive genetic effects were present in the material under study. However the ratio of ?2gca/ ?2sca suggested that the preponderance of non additive gene action in expression of all the characters under study. Out of the nine parents PUSA CHARI-121, PANT CHARI-4, MP CHARI, PANT CHARI-6 and PANT CHARI-5 identified as good general combiner for grain yield/plant, dry fodder yield/plant and other agronomical traits. The hybrid HC-136 x PANT CHARI-4 and PANT CHARI-4 x PUSA CHARI-121 for grain yield/plant and hybrid MP CHARI x PANT CHARI-6, PANT CHARI-5 x SPV 1616 and HC-136 x PANT CHARI-4 for dry fodder yield/plant exhibited higher magnitude of positive significant specific combining ability effect with highest standard heterosis and per se performance. These hybrids were also found suitable for two or three yield contributing traits. In general, close association between specific combining ability effects and standard heterosis was observed among the best hybrids identified on the basis of specific combining ability effects for grain and dry fodder yield

    Comparative assessment of microbial enzyme activity with compost and sewage sludge amendment

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    Changes in soil microbial activities were investigated to examine the effect of aerobically digested sewage sludge (SS) and compared with compost under incubation conditions over 63 days. Sandy soil was amended with 0.25, 0.5, 1.0 and 1.5 % w/w of compost and sewage sludge. Enzyme activity (dehydrogenase, alkaline phosphatase, acid phosphatase, phytase and urease) were examined at an interval of 3, 7, 14, 21, 28, 42 and 63 days. At the end of the experiment the change in organic carbon, nitrogen, potassium and phosphorus was also recorded.Results indicated that enzyme activities were substantially enhanced in presence of both amendments for first few days and the higher increases were measured at 1.5% of compost and sewage sludge amendment. Then an overall decrease in enzyme activity was recorded. Both the amendments also significantly increased the organic carbon, nitrogen and potassium of the soil while increase in available phosphorus was only recorded in treatment receiving compost. The present experiment indicated that addition of compost and sewage sludge have positive effect on soil microbial activity and can be safely used as soil amendment without having any adverse effect. Though, a previous examination of sewage sludge to be used must be made for heavy metals and pathogens

    Response of bio-regulators to yield and quality of Indian mustard (Brassica juncea L. Czernj. and Cosson) under different irrigation environments

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    A field study was conducted during rabi seasons of 2008 and 2009 to evaluated of productivity of Indian mustard (Brassica juncea L.) by foliar spray of bio-regulators under different irrigation environments. Amongst treatments, the application of sufficient irrigation water (four irrigations) recorded the highest number siliqua per plant, higher number of seeds per siliqua, 1000 seed weight and yields as compared to all other irrigation treatments. Results show that an increased in seed yield of 92.40 and 39.37 % under sufficient irrigation and limitedwater condition in 2008 and 93.34% and 40.31% in 2009, respectively over deficient irrigation conditions, foliar sprays of 100 ppm thioglycollic acid at initiation of branching were recorded highest yield attributes and yields. Similarly, 500 ppm of thiourea at initiation of branching and flowering stages also gave second best highest yield attributes and yields as compared to all other bio-regulators spray stages. However, bio-regulators sprays were not recorded any significant effect on oil and protein content. The results were concluded that economic feasibility to improve productivity of Indian mustard with four irrigations levels and sprays of 100 ppm thioglycollic acid and 500 ppm of thiourea at initiation of branching and flowering stages under hyper arid environment

    Deterrent effect of plant extracts against Callosobruchus maculatus on stored cowpea in Saurashtra (Gujarat, India)

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    A laboratory study was conducted at the JAU, Junagadh (Gujarat) during 2013-14 on the deterrent effect of different aqueous plant extracts against pulse beetle, Callosobruchus maculatus (Fabricius) (Coleoptera: Bruchidae) in stored seeds of cowpea, Vigna unguiculata (L.). Among different aqueous extracts of plants materials, maximum percentage of oviposition deterrence was observed in custard apple seed extract (67.19%), custard apple leaf extract (65.95%), neem seed extract (65.44%), mustard seed extract (64.65%) and jatropha leaf extract (64.16%) at 5 per cent concentration. The maximum adult emergence reduction was seen in custard apple seed extract (78.45%), custard apple leaf extract (77.14%) and neem seed kernel extract (NSKE) (77.09%) at 5 per cent dose level. Minimum weight loss percentage was observed in the cowpea seeds treated with custard apple seed extract at 5% dose level, which recorded 79.20%. The results revealed that all of the tested materials with some variations had deterrent and toxic effects against the pest

    In-Vitro androgenesis in papaya (Carica papaya L.) cv. Pusa Nanha

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    Papaya (Carica papaya L.) is an economically important fruit crop of tropics and subtropics. It has high nutritional value, as well as medicinal and industrial applications. Papaya is a polygamous species with three sex types male, female, and hermaphrodite. Conventional methods of papaya breeding are time consuming and needs advent of anther culture which may be effective for shortening of breeding cycles. The present study on in vitro androgenesis in papaya cv. Pusa Nanha observed the highest embryo induction rate (8.0%) when anthers werecultured on agar medium with 0.1 mg/L BA and 0.1 mg/L NAA after incubation in liquid MS medium with 2.0% sucrose for 7 days at 35ºC. The high temperature (35ºC) was more suitable for embryo induction in papaya than slightly low temperature (25ºC). At these both temperatures longer incubation of anthers in water reduced embryo induction rate. Sugar starvation results were ambiguous. Shoots were also developed in the media when used in liquid form. The highest rooting (75.0%) was observed at 2.0 mg/L IBA. Increasing IBA concentration reduced rooting. All well rooted plants were hardened in hardening chamber and successfully transferred to field. The present findings indicated that anther culture can be efficiently contributed for the direct micro-propagation of papaya plants. This study would also be helpful to the researchers to develop more efficient anther culture protocols for further improvement of papaya through in vitro androgenesis

    Effect of botanicals and insecticides seed treatment and containers on seed longevity of black gram under natural ageing conditions

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    Good storage is the basic requirement in seed production programme as maintenance of high seed viability and vigour from harvest to planting is of utmost importance in a seed production programme. Therefore, inexpensive, simple and practicable technology to prolong the shelf life of seeds under ambient condition is immensely needed. Hence, an experiment was carried out to study the effect of seed treatment with neem oil, nimbicidin, emamectin benzoate 5 SG, deltamethrin 2.8 EC, novuluron EC on black gram seed quality under ambient conditions. The seeds without any seed treatment were included as control. The treated and untreated seeds were stored in cloth bag and polylined cloth bag. The results revealed that, botanicals and emamectin benzoate seed treatments were significantly superior in controlling the storage insect and maintaining higher seed quality up to 10 months of storage when compared to control. Among the chemicals, the emamectin benzoate 5 SG @ 40 mg kg-1 of seed found better by recording significantly higher germination percentage (80.28), vigour index I (2124) and dry weight of seedlings (43.14 mg) and lower electrical conductivity (0.939 dSm-1) and seed damage (0.00 %) compared to control (78.78%, 2012, 38.90 mg, 0.942 dSm-1, respectively) at the end of 10th month of storage. The emamectin benzoate 5 SG and nimbicidintreated seeds stored in polylined cloth bags were considered as effective seed storage management approach in blackgram

    Effect of nutritional supplements on queen cell production in honey bee (Apis mellifera)

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    Honey bees are an important means of earning a living both at small and commercial levels. Maximum benefits can be obtained from strong colonies and in order to maintain strong colonies a good beekeeper requeens the colonies every second year. This requires a number of queens. The advances in beekeeping technology have made it possible to rear queens artificially or naturally. There is scope for improvement of these methods. The aim of the present study was to investigate if nutritional supplements could facilitate queen cell production in spring and autumn seasons. Becosule, thiamine, yeast and sugar solutions were fed to the honey bee colonies. The greatest number of queen cells was produced in the yeast fed colonies in spring. Bee mortality was observed in case of becosule. Perhaps the formulation contained some components which were toxic to honey bees. The effect on queen cell production by the different nutrients was in the order of Yeast > Thiamine > Becosule

    Phytoplanktonic diversity in lake Jaisamand, Rajasthan (India)

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    The present study describes the diversity of phytoplankton in the lake Jaisamand of Rajasthan (India). Results depict that phytoplankton was contributed by six major groups which comprised total 85 species. Out of which 13 belongs to Myxophyceae, 5 to Euglenophyceae, 38 to Chlorophyceae, 3 to Xanthophyceae, 1 to Cryptophyceae and 23 to Bacillariophyceae. Thus, Bacillariophyceae and Chlorophyceae turned up as the dominant groups in terms of density (159-554 numbers per liter and 24-485 numbers per liter) and species number (23 and 38), respectively. It was revealed that Jaisamand Lake maintained fairly good population of phytoplankton which is indication of providing broad base for achieving high productivity in this water body

    Efficacy of different oils used for the extraction of annatto colour from the seeds of Bixa orellana L.

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    Annatto, a naturally obtained colour is a potential and valuable Non Wood Forest Product (NWFP). It is obtained from the seeds of plant species Bixa orellana L. The present experiment was carried out to find the efficacy of different oils used for annatto colour extraction. Experiment was started with the pretreatment of seeds of B. orellana with soaking in water at 0%, 50%, 100% and 150% (v/w) for 24 hrs at room temperature and colour extraction with castor oil at 100°C showed that maximum bixin (%) was found from the pretreated seeds with 50% (v/w) water soaking i.e., 0.46% bixin. Similarly for oil soluble annatto extraction method, the maximum extraction of bixin (0.99 %) was found with castor oil at 120°C for 1 hr and least bixin (0.03 %) was recorded with mustard oil at 60oC temperature for 1 hr. Sesame oil and ground nut oil were also showed better extractability. Out of different treatments, castor oil at 120°C for 1 hr gave better result with respect to bixin extraction as compared to others

    Management of early blight of potato using bio control agents and plant extracts

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    The early blight of potato is mainly controlled by using chemical fungicides but chemical fungicides have got some adverse effect on environment as well as human beings. Keeping this in mind an experiment was conducted to test the efficacy of four plant extracts (tea, garlic, onion and neem leaf extract) and four bio-control agents (Trichoderma viride, Pseudomonas fluorescence, Streptomyces graseoviridis and Bacillus substilis) in controlling early blight of potato under field and in in vitro condition. These antifungal compounds were applied 3 times at 7days interval after first appearance of the disease in the field. Among the bio control agents T. viride was found highly effective in per cent disease reduction (52.39%) of disease over control treatment. The tuber yield (25.51 t/ ha.) was also highest in this treatment compared to control treatment (19.53 t/ha). This was followed by treatment T2 i.e. P. fluorescens where per cent incidence and intensity of disease were (65.00%) and (19.10%) respectively with 38.97% reduction of disease over control with tuber yield 23.65 t/ha. It was followed by S. graseoviridis where per cent incidence and intensity of disease were (68.00%) and (22.90%) respectively with 26.30% reduction of disease over control with tuber yield 21.07 t/ha. Among plant extracts, only neem leaf extract exhibited per cent reduction of disease (33.18%) over control treatment in field condition and inhibition of radial growth (59.85%) and spore germination (81.95%) in in vitro condition. Therefore both T. viride and neem can be used for managing the early blight of potato

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