Journal of Applied and Natural Science
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    Intercropping in cabbage (Brassica Oleracea L.var. capitata f.) for growth, yield, quality and sustainable soil health under foothills of Eastern himalayan region

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    The present study was done for intercropping in cabbage (Brassica Oleracea L.var.capitata f.) for growth, yield, quality and sustainable soil health under foothills of Eastern Himalayan region. The pooled results revealed that head yield, ascorbic acid content and nutrient uptake from soil varied according to treatments combination. Sole cropping of cabbage recorded maximum values for most of the growth characters like plant height (1.30 cm), shoot weight (59.47 g), root weight (46.36 g), shoot to root ratio (1.30), root length (17.97 cm) and root volume (37.24 cc) and yield (37.11 t ha-1). But as per cabbage equivalent yield (44.39 t ha-1),economics (B:C ratio 2.96) and production efficiency (591.80 kg-1ha-1day) are concerned cabbage and garden pea intercropping system recorded significantly highest values than all other treatments. cabbage and garden pea intercropping system also recorded maximum ascorbic content(38.61mg-1 100g).Highest residual N (174.12 kg ha-1) content was obtained in sole garden pea plots whereas maximum values for available P2O5 (30.31 kg ha-1) and K2O (171.83 kg ha-1) were recorded in sole coriander plots. Cabbage and beet root intercropping system recorded minimum values for most of the growth characters like shoot weight (35.88 g), root weight (26.80 g),root length(10.83 cm), shoot and root volume (23.54 cc),cabbage equivalent yield (32.10 t ha-1) ascorbic content(29.94 mg-1 100g). Inclusion of garden pea in cabbage inter rows may be adopted by cabbage growers of foothills of eastern Himalayan region of West Bengal for additional income and soil enrichment

    Biology and feeding potential of Coccinella septempunctata (Linn.) against Lipaphis erysimi (Kalt) at different temperature regimes

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    Biology and feeding potential of Coccinella septempunctata (Linn.) were studied in laboratory on 3rd instar nymphs of mustard aphid (Lipaphis erysimi Kalt.) at two different temperature regimes. At 30± 20C, fecundity, oviposition period, hatchability of eggs, male and female longevity (232±70.46, 12.8±3.91, 82.87±6.66, 22.70 ± 2.21and 26.60 ± 4.45, respectively) were higher and incubation period, pre-oviposition period, total grub duration and pupal duration (2.6±0.51, 6.5±3.53, 8.7 ± 1.63 and 5.1 ± 1.10, respectively) were lesser in comparison to 25± 20C where fecundity, oviposition period, hatchability of eggs, male and female longevity, incubation period, preoviposition period, total grub duration and pupal duration were 169.8±61.12, 7.9±1.37, 71.68±4.08, 16.2 ± 1.31 and 21.50 ± 1.95, 2.9±0.73, 7.7±2.49, 11.1 ± 1.52 and 5.8 ± 0.918, respectively . At 27± 2 0C, total mustard aphid consumption by a grub of C. septempunctata during the whole life span, was 424.4±2.78 mustard aphids in comparison to 272.64±1.79 mustard aphid at 23± 20C. Similarly an adult male and female consumed 103.2±1.52 and 116.6 ±1.46 aphids per day respectively at 27± 2 0C in comparison to 65.6 ± 1.02 and 71.8 ± 1.60 at 23± 20C. Thus, at both temperatures aphid consumption increased gradually till 4th instar and 30±20C was found to be most suitable temperature as compared to 25±20C for the life history of C. septempunctata. Hence, it could be concluded that C. septempunctata might play a suitable role in biointensive Integrated Pest Management programme because of better longevity and high predatory potential against the mustard aphid

    Long-term effects of fertilizer and manure application on soil quality and sustainability of jute-rice-wheat production system in Indo-Gangetic plain

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    A long-term fertilizer experiment was initiated in 1971 in sandy loam soil (Eutrochrept) of Barrackpore, West Bengal to study the effects of applying organic and inorganic sources of nutrients on yield of jute-rice-wheat system and soil health. The unfertilized soil supported yields of 0.8 t ha-1 of jute fibre, 1.5 t ha-1 of rice grain and 0.7 t ha-1 of wheat grain (average yield of 42 years). Application of 150% recommended NPK through chemical fertilizers produced maximum yields of jute (2.1 t ha-1), rice (3.8 t ha-1) and wheat (2.8 t ha-1). The yields obtained with 150% NPK fertilizers were 5%, 2.7% and 12% higher than that with 100% NPK fertilizers +FYM. Combined application of 100% NPK fertilizers and FYM, however, increased soil organic carbon, available nitrogen, phosphorus and potassium from 5.60 to 8.90 g kg-1, 270 to 316 kg ha-1, 40.7 to 120 kg ha-1 and 139 to 236 kg ha-1 respectively. Maximum DTPA-extractable micronutrients in soil were also observed with 100%NPK fertilizers+FYM. Applying FYM together with NPK fertilizers increased microbial biomass from 221 to 435 mg kg-1 and microbial quotient from 3.95 to 4.89 with concomitant increase in dehydrogenase, phosphatase and fluorescein-diacetate-hydrolyzing activities in the soil. The acid phosphatase activity (139 to 275 ?g PNPg-1 h-1) was much lower than alkaline phosphatase activity  (479 to 616 ?g PNPg-1 h-1). The enzymes assayed showed significant correlation with microbial-C and organic C. Beneficial effects of integrated nutrient management (NPK+FYM) on soil health were reflected on the yields of all the crops

    Effect of soybean plant phenols and flavonoid on the mean leaf area consumed by Spodopteralitura and Spilosoma obliqua larvae

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    The aim of the present investigation was to study the effect of soybean plant phenols and flavonoid content on the mean leaf area consumed by Spodopteralitura and Spilosoma obliqua larva. Phenols and flavonoid content in methanolic leaf extract of thirty three genotypes of soybean were determined by spectrophotometrically. Thehighest and lowest phenolic content were found in genotypes JS-20-41(2.2±0.073 mg/g) and CSB 904 (0.45 ±0.11 mg/g), respectively. While the highest and lowest flavonoid content was found in genotypes SL 979 4.686± 0.062 mg QE/ g, respectively. In correlation study a highly significant negative correlation was observed between mean leaf area consumed (cm2) by S. litura, phenol content (-0.741 ) and flavonoid content (-0.737) similarly a highly significant negative correlation was observed between mean leaf area consumed by S. obliqua, phenol content (-0.728) and flavonoid content (-0.736) in leaves. Hence it can be concluded that, the genotypes which were having higher phenol and flavonoid content in their leaves offered resistance against S. litura and S. boliqua in soybean

    A note on natural population levels of Phthirapteran species on sheep at district Rampur (U. P.), India

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    A look on the available literature indicated that population characteristics of Phthiraptera on Indian sheep deserved investigation. Two hundred sheep were sampled to reveal the population levels of phthirapteran species on sheep in the district Rampur (U.P.). Three phthirapteran species were recovered (Bovicola ovis Schrank, the facelouse, Linognathus ovillus Neumann and the foot louse of sheep, Linognathus pedalis Osborn). The prevalence to Phthiraptera on sheep was 26.5%, (n= 200) during 2007. The difference in the prevalence of Phthiraptera on two sexes of sheep was not found significant at 5% level. Likewise, the difference in the prevalence of phthirapteran species on young, adult and older sheep was also insignificant at 5% level. The prevalence and intensity of infestation of Phthiraptera were found significantly correlated (at 5% level) to mean monthly temperature. The correlation between prevalence and relative humidity was not found significant at 5% level. The present report provides first information on the population characteristics of phthirapteran ectoparasites infesting Indian sheep

    Effect of deficit irrigation and in situ moisture conservation on soil moisture content and frequency of irrigation in kiwifruit cultivar Allison

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    The effect of deficit irrigation and in situ moisture conservation in kiwifruit cv. Allison vines was studied during the years 2011 and 2012 in the Department of Fruit Science, Dr. Y. S. Parmar University of Horticulture and Forestry, Solan, HP, India. Soil moisture content and frequency of irrigation were investigated in kiwifruit in response to deficit irrigation and in situ moisture conservation techniques. Seven treatments viz., irrigation at 80 per cent Field Capacity (T1), 60 per cent Field Capacity (T2) and 40 per cent Field Capacity (T3), 60 per cent Field Capacity (FC) plus grass mulch (T4) or black polythene (T5) and 40 per cent FC plus grass mulch (T6) or black polythene (T7) were applied from March to October with three replications in Randomized Block Design (RBD). During the year 2011, the soil moisture content under kiwifruit vines was highest under the treatment T1 (15.3, 16.9) , followed by T5 (15.2, 16.8) and T4 (14.9, 16.6) at 30 cm and at 60 cm soil depth, respectively. Whereas, during the year 2012, the soil moisture content under kiwifruit vines was highest under the treatment T1 (14.9, 16.4), followed by T5 (15.0, 16.3) and T4 (14.6, 16.1) at 30 cm and at 60 cm soil depth, respectively. However,the least soil moisture content was, however, observed under T3 (11.0, 12.8) at 30 cm and 60 cm soil depth , respectively, during the year 2011, similarly, during the year 2012, the least soil moisture content was also observed under T3 (10.6, 12.7) at 30 cm and 60 cm soil depth, respectively. The frequency of irrigation was highest under T1 (16 irrigations) followed T2 (10 irrigations) while the least was recorded under T6 and T7 (7irrigations). Total numbers of irrigations applied were reduced from 16 (under T1) to 8 (under T5). The use of black plastic mulch may be beneficial as it helped to conserve moistureunder DI regime which is comparable to those in well irrigated vines. It may also reduce the high irrigation requirement of kiwifruit in areas where sufficient water is not available

    Economic impact of zero tillage on wheat cultivation in Ambala (Haryana), India

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    The present study was conducted with the specific objective i.e. to analyze the economic impact of recourse conservation technology (zero tillage) as compare to conventional tillage practices on wheat cultivation in Ambala district of Haryana. The study revealed that there was significant impact of conservation practices, 22% human labour, 37% machine labour, 25% seed cost and 33% irrigation water was saved under zero tillage compared to conventional tillage (CT) method of wheat production. B: C ratio under zero tillage was 2.86 while underconventional method it was reduced to 2.56. Therefore, zero tillage was economically feasible. It was observed that through the zero tillage farmers can get 3% more yield. The net returns in ZT of wheat production were higher by 4% as compared to CT method. In case of zero tillage, economic efficiency measure was 0.61 as compared to 0.34 in conventional system of wheat cultivation. The research work conducted was holistic in nature combining various elements of technology, resource conservation and economics

    Studies on diversity and abundance of parasitoids of Chromatomyia horticola (Goureau) (Agromyzidae: Diptera) in north-western Himalayas, India

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    Pea leafminer, Chromatomyia horticola (Goureau) is an important pest of many vegetable and ornamental crops. The present investigation was carried out to study the parasitoid diversity of this pest in different agroclimatic conditions of Himachal Pradesh, India. Sixteen species of parasitoids viz. Diglyphus horticola Khan, Diglyphus isaea (Walker), Zagrammosoma sp., Pnigalio sp., Quadrastichus plaquoi Reina and LaSalle, Asecodes erxias (Walker), Closterocerus sp., Neochrysocharis formosa (Westwood), Chrysocharis sp, Chrysocharis indicus Khan, Pediobius indicus Khan (Eulophidae), Opius exiguus (Wesmael), Dacnusa sp. (Braconidae), Cyrtogaster sp., Sphegigaster sp. (Pteromalidae), and Gronotoma sp. (Figitidae) were recorded parasitizing C. horticola in different agro-climatic zones of Himachal Pradesh. Agro-climatic zone II (sub-temperate mid-hills) was the richest in parasitoid diversity (14 species) followed by zone I (11 species), zone III (7 species) and zone IV (4 species) which are characterized by sub-tropical sub-montane, wet temperate high hills and dry temperate high hills, respectively. Shannon diversity index, species richness, species evenness and species dominance varied from 0.69-1.71, 1.39-2.64, 0.50-0.71 and 0.29-0.50, respectively. D. isaea and D. horticola were the dominant parasitoids of C. horticola contributing 41.46-80.15 and 9.16-50.65 per cent of the total parasitization, respectively, in different agro climatic zones. The study highlights the role of different parasitoids in natural control of the leaf miner and will be useful for designing the IPM strategies for the pest

    In vitro evaluation of fungicides against Colletotrichum gloeosporioides (Penz.) Penz and Sacc. causing anthracnose of pomegranate (Punica granatum L.)

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    Pomegranate (Punica granatum L.) is a widely grown fruit in many regions of the world. Anthracnose of pomegranate is one of the limiting factor for low productivity and also the low market price. Therefore, the management of anthracnose disease is necessary. In this study new fungicide molecules are evaluated under in vitro condition against the mycelial growth of Colletotrichum gloeosporioides. In vitro screening of fungicides against C. gloeosporioides showed two combination product Hexaconazole + Zineb, Trifloxystrobin + Tebuconazole and a nonsystemic fungicide Captan showed cent percent inhibition at 100, 250, 500 and 1000 ppm concentration. Similarly, systemic fungicides Hexaconazole, Propiconazole, Penconazole, Tebuconazole and Carbendazim showed cent percent mycelial inhibition at 500, 1000 and 2000 ppm concentrations

    Isolation and characterization of outer membrane proteins (OMPs) from Salmonella Gallinarum in chicken and antibiogram of the isolates

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    Salmonella isolates should be distinguished as it may assist in tracing the source of an outbreak and monitoring trends in antimicrobial resistance associated with a particular type. The specific detection of these Salmonella serotypes is therefore extremely important in order to attribute an isolate to a previously known epidemic outbreak. The present investigation was to isolate and identify S. Gallinarum, to study variation in the profile of outer membrane proteins (OMPs) and to determine in vitro antibiogram of S. Gallinarum in poultry. A total of 228 faecal samples and 22 visceral samples suspected for Salmonellosis were collected, of these 15 samples (6.0%) were found positive for S. Gallinarum. In the present study, rfbS gene sequence was helpful in the serotype-specific detection of S. Gallinarum giving a 187 bp product. Salmonella Gallinarum crude protein extracts determined by SDSPAGE showed migration of OMPs as several bands at approximate moleculer weights of appx. 45 kDa, 55 kDa, 64 kDa, 65 kDa, 74 kDa, 110 kDa, 120 kDa, 135 kDa, 150 kDa,155 kDa, 200 kDa and above 200 kDa. The study indicated a definite variation in the profile of OMPs of various Salmonella Gallinarum strains with major OMPs in the range of appx 80-100 kDa which could be the target for vaccine production. All the isolates tested against 14 antimicrobial agents showed variable susceptibility pattern with highest resistance to nalidixic acid, ampicillin and sulphadiazine and sensitivity to chloramphenicol, gentamicin and enrofloxacin

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