Journal of Applied and Natural Science
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Studies on physico-chemical constituents in different cultivars of citrus fruits under Lucknow condition, India
Get PDFThe physico-chemical studies were conducted on the citrus fruits of sweet orange cv. Mosambi Local-1, 2, 3, 4 and 5 and Sweet Mandarin cv. Chinese Orange, Kinnow, Nagpur Mandarin, Local Mandarin-1 and Local Mandarin- 2. The Sweet Orange cultivar was highest size in Mosambi Local-3 with maximum fruit volume 204 ml and flesh weight 139.66 g followed by Mosambi Local-4. The juice recovery percent was maximum in Mosambi Local-3 28.63 and the highest specific gravity was found in Mosambi Local-1 (0.99) followed by Mosambi Local-2 (0.94). The different cultivars of Sweet Orange were recorded varies from the total soluble solid 7.60 to 12.66 percent, reducing sugar 4.13 to 7.08 per cent, total sugar 5.90 to 10.12 per cent, tritrable acidity 0.52 to 0.83 per cent, ascorbic acid 46 to 74.80 mg/100g and pH 3.92 to 4.98. The juice percent was determined in Kinnow 53.20 g after that Nagpur santra 51.30 g. The chemical attributes of Sweet Mandarin were found as total soluble solid varied from 8.96 to 12.20 per cent, reducing sugar 3.22 to 6.60 per cent, total sugar 5.73 to 10.04 per cent, titrable acidity 0.84 to 2.24 per cent, ascorbic acid 25.96 to 36.13mg/100 g and pH 3.84 to 4.60. However, the Sweet Orange cultivars Mosambi Local-3 and Mosambi Local-4 and Sweet Mandarin cv. Local Mandarin-1 were suitable for export, making of non alcoholic beverages, easy for bottling and frozen products grown under Lucknow conditions
Economic evaluation of conservation tillage options for deciding the feasibility of their adoption
Get PDFThe present experiment was conducted to evaluate two important conservation tillage practices namely zero tillage in wheat and bed planting for growing vegetables for deciding the economic feasibility of their adoption in Gohana and Kharkhoda block of Sonipat district in Haryana. In first scenario, comparison were made between Puddled transplanted rice followed by conventional tilled wheat (PTR – CTW), and puddled transplanted rice followed by zero till wheat (PTR – ZTW). In second scenario, conventionally tilled Jowar in kharif followed by conventionally tilled wheat system (CTJ – CTW) was compared with vegetables on beds in both seasons (BV-BV). Sowing of wheat by zero till drill in PTR – ZTW system, decreased cost of cultivation by 21% over its value of Rs 20688 in PTR – CTW treatment; increased grain yield by 5% over the grain yield of 5.99 t ha-1 in PTR – CTW treatment. The B: C ratio of PTR – ZTW was 4.65 while that of PTR – CTW treatment was 3.24, which indicated economic viability of zero tillage practice. While in case of cauliflower in BV-BV system, the total cost of cultivation was estimated at Rs 51500/ha and net returns of Rs 38072/ha i.e an increased net return of 5.8 times over the net return of Rs 46223.5 in CTJ – CTW treatment. The B: C ratio of BV-BV was 5.21, while that of CTJ – CTW treatment was 2.35. Thus, it was concluded that in rabi season, cauliflower on beds and zero till wheat are most economically viable options in this temporary waterlogged regions of Yamuna basin
Effect of different growth regulators on in vitro micro-propagation of Kufri Frysona
Get PDFIn the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future
Influence of integrated nutrient management practices on dry matter production, yield and NPK uptake of transplanted rice (Oryza sativa)
Get PDFA field investigation was carried out during Rabi season (Pishanam rice) of 2012-2013 at wetland of Central Farm, Agricultural College and Research Institute, TNAU, Killikulam, to study the effect of integrated nutrient management practices on dry matter production, yield and NPK uptake of transplanted rice. The treatments were laid out in a Randomized Block Design and replicated thrice using the rice variety ADT(R) 45. Among the different integrated nutrient management practices, application of GLM @ 6.25 t ha-1 + Azophosmet + 100 % NPK registered significantly the highest (P<0.01) dry matter production at all the stages (Active tillering; 1690, flowering; 9100 and harvest;14490 kg ha-1). The grain yield was increased, when GLM was integrated with 100 % NPK application (6030 kg ha-1). The grain yield was further increased, when Azophosmet was applied through seed and soil application along with GLM and 100 % NPK (6617 kg ha-1). However, it was on par with application of FYM + Azophosmet + 100 % NPK. The same trend was noticed in straw yield also. The uptake of N, P and K nutrients by rice crop at har-vest stages (95.6, 37.7 and 118 kg ha-1) of crop growth was remarkably increased by the application of GLM + Azophosmet + 100 % NPK. Among the various nutrient management practices, application of GLM + Azophosmet + 100 % NPK registered superior growth indicators due to the effective utilization of various nutrients and subsequent accumulation of more assimilates which inturn led to improved vegetative growth and higher yield of transplanted rice
Development of carp fish culture practice under different stocking densities in mid hills of Uttarakhand, India
Get PDFThe present study was undertaken in order to standardise the stocking density values of carps fishes for sustainable fisheries development in mid hills and enhancing the fish production. The paper deals with growth performance of 3 exotic fish species in low stocking density i.e. 3 fish/m3 over high stocking density of 5 fish/m3 and 10 fish/m3 in the control pond. The net production was 12.6% higher with stocking density of 3 fish/m3. Among the both tested density, the combination of 30: 40: 30 was superior (45.6%) in terms of growth and production in comparison to the other combinations and control. In present study, the highest production as 57.13kg/100m2 (5713 kg/ha.) was achieved in the stocking ratio of 30:40:30 with stocking density of 3 fish/m3. It is 4.7% higher of the combination of 40:30:30 and 4% higher than the combination of 30: 30: 40. It is 12.6% higher than the stocking density of 5 fish/m3 and 48.5% higher than the stocking density of 10 fish /m3. The growth pattern reflected the slow growth during the winter months, reflected the direct negative effect of water temperature on the growth. The production level of existing practice of the farmers may be enhanced up to 1.5 times with proper stocking density i.e. 3 fish/m3 and perfect species combination i.e. 30: 40: 30 for silver carp, grass carp and common carp respectively
Mineralization of carbon, nitrogen, phosphorus and sulphur from different organic wastes in silty clay loam soils
Get PDFMineralization of carbon, nitrogen, phosphorus and suphur was studied by incubating soil samples for 90 days at 30°C temperature with four different organic materials viz. Press mud, sugarcane trash, paper mill bagasse and pine needle. The results showed that the carbon mineralization in soil was significantly higher from sugarcane trash followed by press mud as compared to control. The cumulative percent of carbon mineralization increased with incubation period and maximum mineralization was recorded at 90 DOI (days after incubation). The maximum cumulative percent N-mineralization (16.88%) in soil was shown by paper mill bagasse followed by sugarcane trash and pine needle. The percent N-mineralization from all added organic amendments increased incubation period up to 45days of incubation DOI (days after incubation) after then it gradually declined, while the pine needles showed maximum cumulative P-mineralization in soil followed by sugarcane trash while minimum was recorded from press mud. The paper mill bagasse showed highest cumulative S-mineralization in soil followed by sugarcane trash. Irrespective of organic wastes, cumulative S-mineralization significantly increased after 15 days of incubation up to 45 days later on it showed declined trend. Among the organic wastes, sugarcane trash showed maximum Cmineralization in soil exhibited fast decomposition in comparison to other wastes. So, it can be used for composting.The paper mill bagasse showed more N and S mineralization while maximum mineralization of P was found in pine needle. The press mud and sugarcane trash showed potential for short duration enriched compost
Studies on nitrogenase activity of diazotrophic isolates from different rice production systems
Get PDFThe present study was carried out to evaluate the nitrogen fixing ability of diazotrophs isolated from the rhizosphere soils of rice which were grown in three different rice growing systems. A total of hundred and ten isolates obtained were subjected to Acetylene Reduction Assay (ARA) and ninety eight isolates recorded significant amount of nitrogenase activity in a range of 185.73 to 3794.55 nmoles of ethylene mg of protein-1 h-1. The highest nitrogenase activity was recorded by Derxia (3794.55 nmoles of ethylene mg of protein-1 h-1) isolated from Trichy (lowland). Among the three different rice production systems, isolates obtained from lowland rice (Derxia – 3794.5 nmoles of ethylene mg of protein-1 h-1) recorded higher nitrogenase activity followed by Aerobic (Pseudomonas - 2194.89 nmoles of ethylene mg of protein-1 h-1) and SRI (Azotobacter - 1971.85 nmoles of ethylene mg of protein-1 h-1) rice isolates. The results revealed marked variation in the ARA of the diazotrophic isolates obtained from lowland, SRI and Aerobic rice. The nitrogenase activity of diazotrophs from rice fields have been reported earlier but the nitrogenase activity of diazotrophs from three different rice production systems from various parts of Tamil Nadu is reported for the first time from India
Diet composition of freshwater crab, Potamon koolooense Rathbun, 1904 from hillstream of Uttarakhand
Get PDFThe present study was carried out to study the diet composition of freshwater crab, P. koolooense in hillstream Khoh of Uttarakhand during November, 2013 to October, 2014. Total 105 crab samples were used for the study, 35 male and 47 female were found with food in their stomach while rest of the 23 had empty stomachs. The carapace length (CL) ranged from 12mm to 49mm, carapace width (CW) from 15mm to 59mm and total weight (TW) from 2.08g to 60.12g. Stomach content analysis of the animal showed that the diet of the animal composed of animal matter, plant matter, algae, fungi, debris and unidentified materials. Animal matter ranged from (3.27-11.93%), plant matter (4.37-14.44%), algae (1.73-6.15%), fungi (0.17-1.43%), debris (28.18-40.47%) and unidentified materials (36.46-50.05%) in males while in the female animal matter ranged from (3.09-12.34%), plant matter (4.45-12.73%), algae (1.03-7.61%), fungi (0.02-3.34%), debris (23.18-43.34%) and unidentified (30.31-51.04%). Unidentified materials recorded maximum and fungi recorded minimum in both the sexes of Potamon koolooense. A significant difference was observed at p<0.05 between food groups- algae and unidentified matter in both sexes. This is the first report, to key out and quantifies the dietary items and feeding habits of crab in Uttarakhand which would be helpful in interpreting the ecological niche of the animal in mountain stream communities
Induction of reactive oxygen species in brain of Etroplus maculatus after exposure to bisphenol A
Get PDFThe present study was aimed to investigate that bisphenol A, an environmental estrogen, exposed at 648 ?g/ L concentration for 96 h could induce reactive oxygen species in brain of cichlid fish, Etroplus maculatus. Evaluation of antioxidant enzymes as superoxide dismutase, catalase and glutathione reductase showed a reductionin the activities at 5% level of significance with concomitant increase in the level of hydrogen peroxide generation (from 1.546 ± 0.426 (control), 1.506 ± 0.217 (DMSO) to 1.888 ± 0.368 (24 h), 5.332 ± 0.589 (72 h), 2.392 ± 0.341 (96 h)) and lipid peroxidation (from 2.805 ± 0.33 (control), 2.401 ± 0.309 (DMSO) to 3.596 ±0.373 (24 h), 5.65 ± 0.306 (72 h), 3.834 ± 0.236 (96 h)). The brain marker enzyme, acetylcholinesterase decreased at 24, 72 and 96 h in time-dependent manner than that of control groups. The present findings summarize that the increased production of oxygen free radicals due to the exposure of an environmental estrogen, bisphenol A at sub lethal concentration (648 ?g/ L) for 96 h inhibited the activities of antioxidant enzymes thereby induced oxidative stress in brain of fish. The decreased activity of brain marker enzyme, acetylcholinesterase reflect the neurotoxicity of bisphenol A in brain of fish, Etroplus maculatus and this could be one of the possible mechanisms of bisphenol A-induced neurodevelopmental disorders in fish
Smothering effect of different crops on weed Malva neglecta Wallr.
Get PDFField study was conducted at experimental farm of Punjab Agricultural University ,Ludhiana (India) during rabi seasons of 2004-05 and 2005-06. The experiment was laid out in randomized block design with fourteen treatments having combination of seven different crops viz. bread wheat, durum wheat, six - rowed barley, two-rowed barley, raya, gobhi sarson, linseed and two weed control treatments i.e. hand weeded and unweeded. The study was planned with an objective to find out the most suitable Rabi crop that can suppress the weeds to maximum extent with minimum reduction in yield as there was no herbicide available which can control the weeds in an effective manner. Minimum weed dry matter accumulation was observed in raya (0.97qha-1 in the weeded plot) whereas maximum dry matter accumulation was observed in bread wheat (8.3qha-1), followed by durum wheat (6.1qha-1), linseed(5.0qha-1), barley (6-row) (4.9qha-1), barley (2-row) (2.6qha-1) and gobhi sarson (2.4qha-1). Raya (Brassica juncea) showed maximum suppressing poten-tial as minimum per cent reduction in crop yield of unweeded over weeded (7.4%) and minimum per cent increase in weed dry matter of unweeded over weeded( 44%) was observed in this crop. Gobhi sarson (Brassica napus) was the next best smothering crop followed by barley (2-row), barley (6-row), linseed, durum wheat and bread wheat, respectively in suppressing the M. neglecta. Two hand weedings treatment proved better in controlling the weeds as compared to unweeded treatment