Journal of Applied and Natural Science
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    G x E evaluation for feed barley genotypes evaluated in country by AMMI analysis

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    AMMI analysis of feed barley genotypes exhibited highly significant effects of environments, genotypes and interactions for both the years. The major portion of the total variance was described by the environmental effects up to 45.6% and 42.3% in respective years. The genotypes effects contributed marginally as of only 8.6% and 6.9% of total variation. The significant interaction effects were partitioned into IPCA1, IPCA2 , IPCA3 and IPCA4; which explained upto 42.4, 18.3, 9.7 and 8.1% of the first year and 32.2, 20.3, 15.6 and 10.5% for second year. The cumulative effect of first two interaction principal components comes out to 60.7% and 52.3% respectively. Maximum genotype yield during study period varied from 49.8 to 48 whereas the lowest yield ranged from 37 to 36.4 q/ha. AMMI stability index identified genotypes G9(BH 972), G15(JB 274) for former and G23(DWRB 109) & G2(KB 1205) for latter year. AMMI distance marked G15(JB 274) & G7(NDB 1561) for first and genotypes G26(UPB 1034) & G23(DWRB 109) for the second year. Desirable genotypes for selection would be G11(PL 871), G27(PL 872) and G23(DWRB 109), G20(BH 946) for respective years a per the GSI score. Genotypes with IPCA-1 scores close to zero identified G1(PL 751), G9(BH 972) and G27(PL 872 ) for first year and G5(RD 2786), G4(NDB 1554) and G24 (UPB 1036) for second year would have wider adaptation to the tested environments as per AMMI graphical plots

    Heterosis in single cross inter and intra-specific hybrids of Desi cotton (Gossipium arboreum and G. herbaceaum) for their seed cotton yield, fibre quality and seed oil content

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    The present investigation was carried out to assess the expression of per se performance and heterotic effect for fibre quality and seed oil content besides seed cotton yield, studied involving ten desi cotton (Gossipium arboreum and G. herbaceaum) genotypes and their 45 cross combinations in half diallel analysis. F-1 hybrids GBhv-282 x G 27 (67.36%), GBhv- 287 x 824 (58.14%), GBhv- 282 x GAM- 173 (35.00%), GBhv- 286 x G 27 (20.50%), and GBhv- 283 x 824 (18.75%) recorded highest per se performance and significant positive standard heterosis while the maximum heterobeltiosis for seed cotton yield per plant was exhibited by the hybrid GBhv- 287 x 824(155.60 %) followed by GBhv- 282 x G 27 (151.29%) and GBhv- 282 x GAM- 173 (130.30%). Similar trend of heterosis for numbers of boll per plant were observed in above hybrids. For fibre quality traits none of the cross showed consistent high performance for all the characters. Cross GBhv- 283 x 824 was exhibited high standard heterosis for 2.5 % span length, fibre strength, fibre elongation percentage as well as for short fibre index (SFI) while cross GBhv- 286 x 824 were promising for 2.5 per cent span length, fibre strength and fibre fineness. In case of oil content intraarboreum crosses resulted as better crosses and among them cross combination 824 x GAM- 173 was best. Desi cotton hybrids are having lower fibre quality and yield. So, improvement for yield and fibre quality of diploid native varieties through heterosis breeding provided better hybrids for rainfed farming

    Factors affecting people’s participation in joint forest management programmes in Kinnaur district of Himachal Pradesh, India

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    The present investigation examined the various factors affecting people\u27s participation in the planning, implementation and maintenance of JFM programmes in the tribal distrct (Kinnaur) of Himachal Pradesh. In total, 10 factors were identified that influence people’s participation in Joint Forest Management (JFM) activities in the study area, which were independently affecting in all of three development blocks. District as a whole factors affecting in decreasing order were Lack of awareness about participatory forest management (66%), lack of co-ordination with forestry officials (64%), non availability of routine funds (56%), lack of training and visit programme (56%), clash between agriculture and JFM activities (54%), lack of emphasis on quick economic activities (49%), improper usufruct sharing (43%) etc. were some of major factors that influenced people’s participation. Policy and development emphasis on these factors, particularly taking into consideration the geography and need based activity in the various development blocks will increase the people’s participation in similar kind of projects

    Diversity analysis of maize inbred lines using DIVA-GIS under temperate ecologies

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    The vagaries of Climate Change variability need to be addressed and as climatic conditions change at particular experimental sites and maize producing regions, mega-environment assignments will need to be reassessed to guide breeders to appropriate new germplasm and target environments . The development of improved germplasm to meet the needs of future generations in light of climate change and population growth is of the upmost importance . Evaluation of the inbred lines from diverse ecosystems would be effective for production of lines with resilience towards climate variability. Hence, with this objective diverse set of inbred lines sourced from all over India were characterized and were evaluated with DIVA-GIS for diversity analysis of maize inbred lines. Grid maps generated for these maize inbred lines for eleven quantitative traits indicated that these lines can be sourced from North and South India. High Shannon diversity index with maximum range of 2.17-3.0, 2.25-3.0, 2.36-3.0, 2.4-4.0, 2.0-3.0, and 2.2-3.0 were recorded for the traits viz; plant height, ear height, grain weight, grain yield, kernel row and protein content respectively indicating the high response of these traits to ecosystem. However, inbred lines were found to be diverse for all the traits except for ears plant-1 (EPP) and they have been sourced from Northern and Southern parts of India while for EPP recorded less diversity index range of 0.4-1.0 indicating source from South India. Interestingly, less diverse inbred lines for all the eleven quantitative traits have been sourced from Indogangetic plains as indicated in diversity grid maps. Maximum diversity indices were recorded for anthesis silking interval (ASI), days to silking, days to tasseling, which are in the range of 0.97-2.0, 1.528-2.0, 1.516-2.0 and 1.528-2.0 respectively. Hence, DIVA-GIS enabled identification of diverse sources from varied ecosystems which can be used for developing improved lines/ cultivars with greater resilience towards climate change

    Development of low alcoholic naturally carbonated fermented debittered beverage from grapefruit (Citrus paradisi)

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    A pure yeast Clavispora lusitaniae, isolated from whey beverage, phenotypically characterized and molecularly characterized by sequencing of D1/D2 domain of 26S rRNA and Internal Transcribed Spacer (ITS) region was used to produce low alcoholic naturally carbonated fermented debittered beverage from Grapefruit. C. lusitaniae produces enzyme naringinase. This enzyme is a mixture of ?-L-rhamnosidase and ?-D-glucosidase. The bitter component in citrus fruit, naringin can be hydrolyzed by ?-L-rhamnosidase to rhamnose and prunin then by ?-glucosidase to glucose and naringenin. The freshly prepared fermented Grapefruit beverage had TSS 14 °B, pH 4.7, acidity 0.26%, brix acid ratio 53.85, total sugars 11.6%, reducing sugars 3.34%, ascorbic acid 21.9 mg/100 ml, naringin 643.2 ppm, alcohol 0.00% (v/v), CO2 0.00 bar and total yeast count 5.83 (Log no.of cells/ml). Physico-chemical changes recorded after three months of storage at refrigerated temperature revealed TSS 12.0 °B, pH 4.2, acidity 0.54%, brix acid ratio 22.22, total sugars 8.97%, reducing sugars 1.94%, ascorbic acid 18.45 mg/100 ml, naringin 365.2 ppm, alcohol 0.76 % (v/v), CO2 1.35 bar and total yeast count 8.54 (Log no.of cells/ml). Naturally produced CO2 by C. lusitaniae during fermentation adds effervescence, sparkle, tangy taste to the beverage in addition to its antimicrobial properties. Thus bio-enzymatic debittering by C. lusitaniae may become the new direction of citrus juice processing in the future, due to its economical viability with strong ability to remove the bitter taste from citrus juice beverage

    Assessment of the effects of land use pattern on distribution of sulphur fractions in soil

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    In the present investigation, various forms of sulphur (S) viz., total S, organic S, inorganic S and available S were estimated in soils under four different land uses viz., Rice-rice, rice-green gram, mango orchard and a fallow. Soils samples were taken up to a profile depth of 0.60m at 3 depths i.e. 0-0.20m, 0.20-0.40m and 0.40-0.60m. Thesoils were found to be slightly acidic to moderately acidic in reaction (5.83-6.59), showing an increase along the depth irrespective of the land use pattern. Soil organic carbon (SOC) content (mean 5.5 gkg-1) was found well above the low level of SOC in soils. Calcium carbonate content, bulk density and clay content of soils didn’t maintain any definite pattern along the depth. All forms of S were found to decrease along depth irrespective of the land use patterns. The available S content ranged from 12.2 to 21.4mgkg-1 of soils. The relative preponderance of all the forms of S followed the order: mango orchard> fallow> rice-rice> rice-green gram. On an average organic S and available S fractions constituted 93 and 6 per cent of total S respectively. A correlation matrix revealed that all the forms of S maintained a significant positive correlation with SOC content while a negative correlation with pH of the soils. The results of the study will be useful in managing the different fractions of S in soils in order to maintain its availability well above the critical level

    Seed traits, germination pattern and seedling vigour in Antiaris toxicaria (Pers.) Lesch., a rare plant species of Western ghats

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    Antiaris toxicaria (Pers.) Lesch. is one of the species of ecological importance distributed in Western Ghats and other parts. This species is considered as one of the threatened species and it needs conservation. The information on fruit and seed trait variation and germination attributes are scanty. Such database is useful for afforestation programme as well as for augmentation of rare plant species in their natural habitat. For the present study, 10 open pollinated trees distributed in Devade forest of Sakharpa were marked. April month was the peak fruiting period and healthy fruits were collected from the ground. Fruit and seed traits were found to be varied significantly among trees, where fruit length ranged from 21.50 to 24.71 mm and fruit weight ranged from 3.64 to 4.07 g. Similarly, seeds length (14.01 -15.09 mm), seed thickness (12.15 -13.04 mm) and seed weight (1.20 – 1.57g) also showed significant variation among 10 trees. Field observation showed that there was a poor regeneration in this species at studied site. Therefore, germination study was conducted at nursery condition by imposing several pretreatments. Among eight treatments including control, soaking seeds in water for 24 hrs (T2) resulted in better germination of 38.0% as compared to others. In T2 seed germination started at 16 days after sowing and completed within 42 days. Potting mixture of red soil with saw dust in 30:70 ratio improved seed germination about 20 % as compared to red soil media

    Study of variance, heritability and genetic advance for various yield contributing and quality traits in spring wheat (Triticum aestivum L.)

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    An experiment was conducted to study the coefficient of variance, heritability and genetic advance for different traits in spring wheat .The investigation comprised of 7 lines of wheat and their 21 crosses in half diallel fashion was carried out in RBD with three replications. Data were recorded for days to 75% heading, days to maturity,plant height, number of productive tillers per plant, spike length, number of spikelets per spike, number of grains per spike, grain weight per spike, 1000-grain weight, biological yield per plant, harvest index, grain yield per plant, protein content and sedimentation value. The mean squares of the analysis of variance revealed significant and highly significant differences among genotypes for characters studied. Both PCV and GCV (21.8% and 21.3%) were highest for biological yield per plant followed by grain yield per plant (20.9%) and harvest index (19.7%) respectively. Whereas, ECV was maximum (15.2%) for grain yield per plant followed by harvest index (12.2%) and lowest value was recorded for days to 75% heading (0.85%). Days to 75% heading was highly heritable (90.94%) trait followed by plant height (87.23%) while least heritability (17.73%) was noticed for number of grain per spike. The highest genetic advance shown by the biological yield per plant (48.33g) followed by grain yield per plant (19.75g), however, the greatest genetic value percent mean 43.084 for the days to maturity and minimum (2.10) for spike length. Hence, these statistical parameters might be given top priority to strengthen the successful breeding program

    Distribution of tomato yellow leaf curl virus (TYLCV) in different parts of tomato plants: A serological perspective

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    Serological detection of tomato yellow leaf curl virus in different parts of tomato through DAS-ELISA revealed that its branches have highest virus concentration followed by twigs, mature leaves and middle leaves. The samples derived from the branches gave highest mean OD value 0.517 whereas, the recorded mean OD values in case of twigs, mature leaves and middle leaves were 0.470, 0.444 and 0.419, at A405 nm, respectively. The virus was not found to be present in flowers and fruits as indicated by lower mean OD values, i.e. 0.215 and 0.159, respectively. On the basis of findings of the our experiment, the branches, twigs, mature leaves and middle leaves had high concentration of the virus and hence these parts could be used in the detection of TYLCV, whereas, flowers and fruits had very low titer of virus, thus, not recommended to be used for serological detection

    Evaluation of the effects of FYM and gypsum on onion (Allium cepa L.) production under sodic water irrigation

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    A field experiment was conducted at CCS Haryana Agricultural University, Hisar to study the effect of FYM and gypsum on onion (Allium cepa L.) production under sodic water irrigation. Treatments comprised of three levels of farm yard manure (FYM) (F0- no FYM, F1- 10 t/ha and F2- 20 t/ha FYM) and three levels of gypsum (G0- nogypsum, G1- 50% neutralization of RSC and G2- 100% neutralization of RSC) in addition to control (irrigation with sodic water and no FYM and no gypsum F0G0). Results of the study showed that the seedling mortality was maximum (77.50 %) in control (F0G0) treatment, while minimum number of seedlings mortality (18.17 %) was observed in (F2G2) treatment followed by (25.17 %) (F2G1) treatment. There was significant increase in growth and yield of onion with application of FYM and gypsum. Moreover, maximum bulb yield (180.83 q/ha) was recorded in (F2G2) followed by F2G1 (152.22 q/ha) where 100% sodicity of water was neutralized by the application of gypsum and 20 t/ha FYM. Under sodic water conditions, increasing level of gypsum and FYM help in reducing the sodicity of irrigation water and thereby, increasing the yield of onion. The study concluded that in present situation of scarcity of good quality water for agricultural purposes, use of amendments like FYM and gypsum were best alternative for the best possible use of poor quality ground water and simultaneously sustain the yield of vegetable crops like onion which are sensitive to sodic water

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