Journal of Applied and Natural Science
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Antibacterial and anticancer activity of green synthesised silver nanoparticles using polysaccharides extracted from the marine alga Portieria hornemannii
Get PDFThe increasing incidence of cancer cases and multi-drug-resistant bacteria, which are major threats to humankind, forces the research world to innovate new molecules to deal with them. The main aim of the present work is to prepare silver nanoparticles using macroalgal polysaccharides and to study biological activities. The silver nanoparticles (NPs) were prepared using polysaccharides extracted from the marine macro alga Portieria hornemannii by stirring them with 1 mM silver nitrate after 24 h at 90 ºC. The formed silver nanoparticles were characterized using UV-visible spectrophotometry, Fourier transform infrared spectroscopy (FTIR) analysis, Transmission Electron Microscopy (TEM) analysis, selected-area electron diffraction (SAED), and Energy Dispersive X-ray (EDX) analysis. UV-visible spectrum analysis revealed a surface plasmon peak at 380 nm, showing the development of silver nanoparticles. The nanoparticle size varied between 40 and 50 nm and the functional group was analyzed using FT-IR spectrum. The broadband was observed at 3304 cm-1 (hydroxyl and amino group) and the narrow band was observed at 2907 cm-1 (C–H stretching vibration), 1657 cm-1 (stretching of carbonyl groups), and 1001 cm-1 (C–O stretching vibration). The crystalline nature of silver NPs was confirmed by SAED. EDX analysis reveals the purity and the chemical composition of silver NPs. Nanoparticles were highly effective against Proteus mirabilis (24 mm zone of inhibition) and Bacillus substilis (24 mm zone of inhibition). The anticancer activity of the silver nanoparticles tested against colorectal adenocarcinoma cell lines increased at increasing concentrations of nanoparticles
Influence of GA3 (gibberellic acid) and Ca(calcium) on root trait variation and osmotic potential of linseed (Linum usitatissimum L.) under chloride-dominated salinity
Get PDFLinseed (Linum usitatissimum) is a versatile crop cultivated for its seeds, which are valuable source of ω-3 fatty acids. It adversely affected by soil salinity, as high salt levels can hinder their growth and reduce yields. To assess the potential for mitigating the adverse effects of high salinity concentrations, enhancing the resilience of three genotypes (Shekhar, Sheela, and Kartika) of linseed plants, this research aimed to find out the impact of Gibberellic acid (GA3) and Calcium (Ca) on various aspects of root morphology, osmotic potential of linseed, under varying levels of Cl- dominated salinity. The study employed three salinity levels (0, 5, and 10 dSm-1) and exogenous application of 10−6 M GA3 and/or 10 mg CaCl2 kg-1 in potted plants.The findings indicated that increasing salinity stress significantly (p≤0.05) affected root parameters, including total surface area(43.45%), average diameter(42.06%), total projected area(44.45%), length per volume (66.23%), root length, total root volume (73.23%), tips, forks,fine roots, and osmotic potential(66.67%). Correlations among linseed genotypes were observed between various root morphology and osmotic potential parameters. The application of GA3 and Ca effectively ameliorated the impact of salinity stress at its highest level (10 dSm-1), resulting in increased root parameters while decreasing the osmotic potential (Ψs). Both GA3 and Ca treatments significantly influenced root architecture and maintained optimal osmotic potential. The chloride-dominated salinity exerted inhibitory effects on all three genotypes’ (Shekhar, Sheela, and Kartika) root growth parameters while applying GA3 and Ca successfully mitigated these effects, enhancing root growth.
A comparative study of essential oil components of selected promising small cardamom (Elettaria cardamomum (L.) Maton) landraces in Cardamom Hill Reserve (CHR) of South India
Get PDFElettaria cardamomum (L.) Maton originated in the evergreen forests of the Western Ghats of South India. In order to compare the percentage of essential oil and its components of selected small cardamom landraces, the study was conducted at the Indian Cardamom Research Institute, Myladumpara, Idukki Dt. of Kerala. Essential oil components of nine farmer\u27s varieties such as Njallani Green Gold, Thiruthali, Panikulangara Green Bold No.1, Wonder Cardamom, Elarajan, Arjun, Pappalu, PNS Vaigai, Pachaikkai; and ICRI-5 as control were compared to evaluate the quality of genotypes. Essential oil content was significantly higher in Najllani Green Gold (9.18%), followed by Wonder Cardamom (8.36%). GC-MS profiling identified twenty-five compounds contributing to 90-93% of essential oil. The higher percentage of 1,8-cineole was observed in PNS Vaigai (38.04%) and the control ICRI-5 (38.99%), while Panikulangara Green Bold No.1 had the lowest percentage (24.30%). α- terpinyl acetate was higher (43.39%) in Panikulangara Green Bold No.1 followed by Thiruthali (39.21%), and was lowest in ICRI-5 (35.97%). Panikulangara Green Bold 1 had a higher percentage of α-terpineol, Geranyl acetate, Geraniol and Linalyl acetate than other genotypes. The percentage of sabinene was higher in Njallani Green Gold (7.56%), and in the genotype, Elarajan (5.46%) was lowest. Due to the medicinal and flavour-contributing properties, the essential oil components can be used in medicinal and food industries.
Evaluation of antidiabetic potential of Hibiscus rosa sinensis on streptozotocin-induced diabetes on Wistar albino rats
Get PDFThe chronic metabolic disease known as diabetes mellitus causes hyperglycemia in the body. Antioxidant and antidiabetic qualities are well-known benefits of Hibiscus rosa sinensis (HRS). In this work, diabetic Wistar albino rats were used to assess the antidiabetic properties of HRS flower extract. A total of 18 animals were taken and divided into three groups (n = 6) – (Group 1): Normal control group, (Group 2): Diabetic control group, (Group 3): Diabetic group treated with a 125 mg/kg dose of HRS flower extract. Group 2 animals showed a progressive decrease in body weight, while Group 1 animals showed a considerable gain in body weight. After overcoming the weight loss, Group 3 animals also showed an increase in body weight that was similar to Group 1. Group 2 animals had blood glucose levels higher than 400 mg/dL, but Group 1 animals had blood glucose levels below 200 mg/dL throughout the experiment. Group 3 animals first had glucose levels higher than 350 mg/dL and then lower than 200 mg/dL, comparable to Group 1 animals. Upon histological examination, the pancreatic islets of Group 2 animals showed vacuolation, necrosis, and degeneration. The animals in Group 3 displayed regenerated islets of Langerhans and enhanced pancreatic anatomy. The animals in Group 3 also returned to normal in terms of body weight and blood glucose levels, similar to those in Group 1. These findings show that Hibiscus rosa sinensis has potential as an alternative diabetic treatment; further research is needed to fully understand its modes of action and long-term effects.
Pycocyanin: A virulence factor of Pseudomonas aeruginosa in the disruption of brain homeostasis regulation in gold fish Carassius auratus
Get PDFRecent reports reported that the oral and gut microorganisms are responsible for the regulation of the brain homeostasis mechanism. This brain homeostasis mechanism is affected by the colonization of non-periodontic microorganisms in the oral cavity and the gut compared to periodontic pathogens. Among the non-periodontic microorganisms, Pseudomonas aeruginosa is one of the gram-negative bacilli that play a major role in the development of cognitive impairment through the production of a secondary metabolite called pycocyanin.The present study aimed to test the effect of pycocyanin on the development of cognitive impairment for the first time with the help of a comparative two-staged behavioral analysis: non-infusive behavioral studies (NBS) and infusive behavioral studies (IBS) of goldfish Carassius auratus. Non-infusive behavioral experimental groups (BEGs) used in the NBS mimicked the condition of a healthy state and infusive behavioral experimental groups (BEGs) either received isolated metabolites and microbial cultures of the day – 3, 4, 5, and 6 in the form of oral infusions. Effect of metabolite/ microbial culture in the infusive study groups was proved by comparing the behavioral scores of non-infusive groups. Employed two-staged behavioral analysis proved that cognitive impairment induction (75-79%) was higher in the metabolite oral infusions compared to the microbial oral infusions in the behavioral study groups. Obtained results showed that induction of cognitive impairment resulted from reactive oxygen species (ROS) production and neuroinflammation was high in the brain regions due to the transportation of administrated metabolite from the gut to the brain in its purest form compared to the microbial oral infusions.
Molecular diagnosis of bacteria isolated from Trifolium repens root nodules
Get PDFThe Fabaceae genus Trifolium comprises around 250 species widely distributed worldwide, with the temperate Northern Hemisphere exhibiting the highest variety. The plants in this genus are widely used as livestock fodder crops and are particularly significant economically.This study\u27s objective included isolating bacteria from the root nodules of the Trifolium repens plant and diagnosing it at the molecular and microbiological levels. T. repens root nodules were used as the source of an endophytic bacteria isolated on Yeast Extract Mannitol (YEM) media that had solidified and diagnosed at the molecular level by DNA Sequencing technique for analysis of the sequence of the nitrogenous bases of 16S rRNA gene with the global database. The isolated bacteria were characteristic of greyish-white color after 48 hours of growth and appeared as a circular shape, slightly convex and gram-negative. The bacteria were resistant to the antibiotics 20µg/ml Aztreonam.The DNA sequencing technique for analysis of the sequence of the nitrogenous bases of 16S rRNA gene with the global database of the National Center for Biotechnology Information (NCBI) showed that the isolated bacteria was at least 96.22% similar to the species Acinetobacter baumannii As a result, it was recorded for the first time as Acinetobacter sp. AZS1 strain in NCBI.
Performance evaluation of Bacopa monneri-loaded ethosomes for topical delivery
Get PDFBacopa monnieri is a plant with a rich history of use in traditional Ayurvedic medicine, spanning several centuries. It is also referred to as Brahmi in some regions. This product serves as a treatment for various skin conditions, such as inflammation and wound healing. Its properties also aid in the production of collagen and improve circulation. The inclusion of antioxidants enhances your skin\u27s overall health, resulting in a rejuvenated and vibrant appearance. The present study aimed to prepare a nano-lipoidal system loaded with Bacopa monnieri (BM) extract and its characterization. Twelve formulations (F1-12) were developed using the ether injection method using different ratios of BM extract, L-alpha phosphatidylcholine (SPC), ethanol, and water. Bacoside A was used as a marker compound for estimation purposes. BM extract-loaded ethosomes were characterized in which formulation F-5 showed the highest entrapment efficiency of 89 %, with vesicle size 188 nm, while the zeta potential was -29.19 mV, and the polydispersity index (PDI) was 0.221±1.45. In vitro extract release using a dialysis membrane was performed for 12 hours, and it was found to be 44 % and 61 % at the end of 8 and 12 hours. The formulation followed zero-order non-Fickian diffusion kinetics, which is best for transdermal formulations. The goat skin was used for Confocal laser scanning microscopy (CLSM) study which showed the fluorescence intensity of Rhodamine B entrapped in those was 16.783, while it was 8.580 in the blank hydroethanolic solution, which confirmed the penetration of the ethosomal systems up to 30-40 µm deeper into the skin which gives a possibility that ethosomes can contribute in collagen synthesis and decrease the degradation of elastin in the deeper layers.
Application of Satellite and Geospatial technology for flash flood mapping over Himalayan with reference to Dharamshala in Himachal Pradesh, India during July 2021
Get PDFFloods have devastating consequences for human life and economies worldwide. The present study focuses on flash flooding in the Himalayan environment, specifically in Dharamshala, Himachal Pradesh, India. In July 2021, a low-pressure system developed in the area, intensifying into a deep depression and resulting in extreme rainfall on July 12, 2021. This extreme rainfall event resulted flash flooding, landslides, and extensive damage in Dharamshala. Utilizing satellite observation and geospatial technology, this study examined the heavy rainfall events and the vulnerability of the geology of Dharamshala to flash flooding. The area received a cumulative rainfall of more than 370 mm, with over 35 mm recorded at 09:00 Universal Time Coordinated (UTC). The steep slopes of the region, combined with sudden heavy downpours from multiple extreme rainfall events, contributed to flash flooding and landslides. The consequences were severe, resulting in the loss of seven lives and impacting thousands of people in the study area. The research findings emphasize the significance of satellite-borne remote sensing applications and geospatial technology for real-time short-lived localised rainfall events in the study area. The study also highlights the role of Bhagsu Nala in exacerbating flood risks. This research provides a valuable model for implementing effective disaster mitigation measures. Moreover, it contributes to global efforts to reduce the risk of natural catastrophes, particularly in Himachal Pradesh. The findings offer valuable insights into disaster management and mitigation strategies.
Influence of frying conditions on colour kinetics and viscosity of frying medium employed for making Indian recipe Gulabjamun
Get PDFFrying Gulabjamun in Ghee is key to its distinct taste and texture, but reheating frequency and conditions can affect the sweet\u27s colour and consistency. It is important to understand this dynamic to ensure the desired quality of the final product. Based on the facts as mentioned above, the present study was conducted to analyse the change in colour and viscosity occurring in the Ghee during repetitive frying to cook Gulabjamuns.The change in colour attributes and viscosity of Ghee was monitored at various hours of frying. The results revealed that the Ghee became darker, the green hues became more prominent, and the yellow hues became more intense the longer it was fried. Besides, the most significant color change occurred during the 60-hour frying duration, as indicated by a decrease in lightness (L* value from 43.07 to 41.1) and a noticeable shift towards greener hues (Δa* value of -7.22334), suggesting that extended frying times lead to the darker and greener side. It attained its maximum viscosity after 12 hours of frying, with a shear stress of 19.10 Pa and viscosity of 0.66 Pa-s, which was correlated with the formation of a scum and the disappearance of steam bubbles. The physicochemical changes taking place in Ghee during the frying of Gulabjamun after repeated heating provide understanding to sweet makers for the selection of the appropriate frying medium and frying techniques, which ultimately assist in the improvement of the overall sensory attributes of the Gulabjamun.
Standardization of packaging material, storage durations and chemical treatments on vase life of soft shield fern Polystichum squarrosum (D. Don)
Get PDFPolystichum species, native to warm-temperate and montane-tropical environments (including some alpine regions), are terrestrial or rock-dwelling ferns. These plants exhibit dark green, leather-like, and lustrous fronds, which are highly valued in floral arrangements due to their aesthetic elegance and longevity. Polystichum squarrosum is not being cultivated commercially but collected from forests for its use as cut green in the florist industry. Hence the collected material should be handled carefully to utilize every frond. Therefore, the present study aimed to develop suitable post-harvest handling procedures for this valued cut green. A Completely Randomized Design (CRD) Factorial layout with 19 treatment modules (M0 to M18) was employed. Treatment modules included packaging material as poly mesh net bags and packing materials as corrugated fiberboard boxes , two storage durations (3 and 6 days) and chemical treatments [glycerol (4%) and Benzyl adenine (25mg L-1)]. Results revealed that spraying cut fronds with 4% glycerol, wrapping them in cellophane sheets and storing them for three days (M9) resulted in significant improvements in appearance, weight change, vase life and water uptake. Fronds from Season-II (March-April 2020) exhibited excellent performance over Season-I (August-September 2019). The interaction between M9 and Season-II further enhanced the postharvest parameters. The combination of glycerol treatment and cellophane wrapping for three-day storage proved most effective in preserving the quality of cut fronds.