Journal of Applied and Natural Science
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Physiological and biochemical response to higher temperature stress in hot pepper (Capsicum annuum L.)
Get PDFThe present study was conducted to evaluate the physiological and biochemical changes in some thermotolerant and thermosensitive chilli (Capsicum annuum L.) genotypes. Fourteen chilli genotypes (SL 461, PP 404, DL 161, MS 341, VR 521, PB 405, PS 403, SD 463, FL 201, AC 102, S 343, SL 462 and SL 464 along with sensitive check [Royal Wonder of bell pepper] were evaluated for heat tolerance. The observations on morpho-physiological and biochemical parameters were recorded at 45, 65, 85 and 105 days after transplanting (DAT) (high temperature period). On the basis of our studies, genotypes S 343, AC 102 and FL 201 were found to be relatively thermotolerant. However, high temperature markedly decreased the photosynthetic activity of chilli plants by decreasing the photosynthetic pigments in leaf chloroplasts of all the genotypes. The levels of ascorbic acid, total soluble sugars and total phenols increased in the leaves of all the genotypes with the maturity of the crop. Electrolyte leakage and proline content also increased with rise in temperature. Genotypes AC 102 and S 343 were able to accumulate the maximum ascorbic acid, proline, total soluble sugars and total phenols under heat stress conditions. Decrease in fruit set percent led to reduction in the total yield per plant. Maximum yield was observed in genotype S 343 followed by FL 201
Development of near isogenic lines (NILS) using backcross method of breeding and simultaneous screening against Karnal bunt disease of wheat
Get PDFKarnal bunt of wheat caused by Tilletia indica can be a hindrance to wheat trade as the fungal disease is known to affect the quality of the grain. As a breeding strategy, a set of eight near isogenics lines (NILs) and backcross derived recombinant inbred lines (micro-RILs) were developed in the background of PBW 343 and WH542 for Karnal bunt resistance over a period of time. The donor stocks resistant to Karnal bunt used in the study were ALDAN ‘ S’ / IAS 58, CMH 77.308, H567.7I, HD29, HP1531, W485 and their derived lines KBRL 22 and KBRL 57. Effective method for screening to Karnal bunt was standardized and used for identification of resistant lines across many seasons. Some of the identified lines evaluated for yield were found to be equivalent to the high yielding parents and the commercially grown check varieties. These developed lines will serve as a basic material for production of Karnal bunt free wheat
Propagation of Himalayan maple (Acer caesium Wall.) through seed and softwood cuttings
Get PDFPropagation of Himalayan maple (Acer caesium Wall.) through seed and softwood cuttings was investigated to standardize nursery techniques for mass production of the species. The seedlings were raised from viable seeds in different containers filled with different combinations of growing media. Vegetative propagation of softwoodcuttings was carried out by treating with different combinations of IBA and Willow leachate of different durations. The statistical analysis revealed the differential behaviour of various nursery stocks with respect to survival, growth and biomass. The growing media soil:sand:vermicompost (2:1:2) and container (root trainer 300 cc) showed maximum germination (61.00%), seedlings height (35.17 cm), collar diameter (5.07 cm), shoot:root ratio (1.24) and survival percentage (76.33%). Conversely, the cuttings treated with IBA @ 8000 ppm showed maximum sprouting (74.50%), rooting (66.75%), length of longest root (14.65 cm), no. of roots per cutting (33.00%), shoot length (13.90 cm) and survival percentage (41.50%). Hence, the seeds of the species should be grown in Rot trainer of 300 cc having soil:sand:vermicompost (2:1:2) to get good quality planting materials. However, the softwood cuttings should be given treatments with IBA @ 8000 ppm for mass production of plants vegetatively
Statistical media optimization studies for growth and polydroxybutyrate (PHB) production by Pseudomonas spp.
Get PDFUsing glucose as carbon source and mustard cake and yeast extract as nitrogen sources bacterial isolate Pseudomonas B2 exhibited a maximum PHB recovery of 0.620 (in terms of O.D.) and PHB weight of 0.27g/L in 96 h. To determine the possibility of growth potential of Pseudomonas spp., it was grown in different carbon sources like fructose, glucose, maltose, mannitol etc. and it was found that glucose yielded good growth and PHB production. In order to incorporate cost effective nitrogen and carbon source, mustard cake and cotton cake as nitrogen source and molasses as carbon were used in medium. Statistical media optimization design was used to optimize the culture conditions for maximizing the PHB production. A maximum of 0.37 g/L of PHB and 0.746 (O.D.) PHB recoveries were obtained using optimized concentrations. Batch kinetics can be used for model development, which will make possible simulation of nutrient limited cultivation(s) for over accumulation of PHB. FTIR studies confirmed the presence of PHB
Partial acidulation of phosphate rock for enhanced phosphorus availability in alluvial soils of Bihar, India
Get PDFThe present study was undertaken to fractionate partially acidulated phosphate rock (PAPR) and evaluate its effect on Phosphorus availability in alluvial soils. For this purpose, low grade phosphate rock was collected from Udaipur, Rajasthan and acidulated at different degrees with sulphuric acid (H2SO4) (v/v)Results from an incubation study showed that the available fraction of P [Water soluble (WSP) and Citrate soluble (CSP)] increased with increasing degree of acidulation. The maximum WSP was obtained at 50% H2SO4 (52.30% of total P) and the maximum CSP was obtained at 80% H2SO4 (49.54% of total P). 50 % dissolution of rock phosphate was the best treatment and found to be at par with that of 60% and 80% but was significantly superior to rest of the treatments. The maximum WSP in the soil samples was obtained after acidulation with 50 %H2SO4 and the maximum Olsen’s P by the 14th day of incubation. An increasing trend was found at initial stages of incubation (7-14 days) with a notable decrease in the later stages. WSP demonstrated the maximum correlation at 40% dissolution(r = 96* for first order reaction, r =95* for second order reaction) while for Olsen’s P, the maximum correlation was found in initial rock phosphate sample (r =88* for first order reaction, r =94* for second order reaction). Kinetics analysis of the obtained rock phosphate demonstrated that the PARP has more influence on phosphorus release pattern as compared to the inorganic P (SSP)
Performance of broccoli (Brassica oleracea var. italica) under drip irrigation and mulch
Get PDFField experiment was conducted at Central Research Farm of Bidhan Chandra Krishi Viswavidyala, Gayespur, West Bengal during winter seasons of 2011-12 and 2012-13 to assess the comparative effectiveness of drip and conventional surface irrigation with and without mulch on growth and yield of broccoli. The experiment was laid out in split-plot design replicated thrice. Main plot treatments consist of four levels of irrigation such as surface irrigation with IW/CPE 1.0 and three drip irrigation at 1.0, 0.8 and 0.6 ETc (crop-evapotranspiration), and three mulch levels like no mulch, black polythene mulch and paddy straw mulch @ 5t/ha in sub-plots. The results showed that drip irrigation at 0.8 ETc showed significantly higher (P = 0.05) plant height (45.69 cm), no of leaves plant-1 (17.66), leaf size index (743.99 cm2), plant spread (89.94 cm), curd diameter (14.43 cm) and marketable curd yield (17.82 t ha-1) of broccoli, which was at par with drip at 1.0 ETc. Minimum growth and yield was obtained with drip irrigation at 0.6 ETc in both the years. Similarly, significantly the highest (P = 0.05) plant variables and curd yield was obtained with use of black polythene mulch over paddy straw and no mulch treatments. However, drip irrigation at 0.6 ETc registered maximum water use efficiency of 117.31kg ha-mm-1 and water saving of 38.43%. The interaction effect showed that drip irrigation at 0.8 ETc along with black polythene mulch produced significantly higher marketable curd yield. The experimental findings can be recommended for growing high value crop broccoli with water saving drip irrigation at ETc 0.8 along with plastic mulch technology in the water scarce regions of West Bengal
Impact of improved farm technology on pulses production in Karnataka
Get PDFThe current study aimed at evaluating technical efficiency and change in output of pulse crops in Karnataka. Totally 180 farmers were selected for eliciting required information on adoption of A3P technology. The results of the study revealed that majority of the farmers were operating above 80 per cent efficiency levels in both pulses across the farmers of A3P and Non-A3P. Further, more than 53 per cent of farmers\u27 under redgram and 56 per cent under bengalgram have achieved 100 per cent efficiency level in A3P farmers category. Decomposition of the difference in total output between A3P and Non-A3P farmers was 13.23 and 14.86 per cent in redgram and bengalgram crops respectively. The A3P technology has alone contributed to the extent of 29 and 22 per cent to the total change in redgram and bengalgram output indicating output can be increased by about 29 and 22 per cent if the farmers could switch over from traditional practices (Non-A3P) to A3P technology. There is need to educate farmers on optimum use of inputs through conducting intensive trainings on A3P technology by KVK’s and other extension agencies to increase the supply of pulses in the country
Zonal trend-agrometeorological models for wheat yield estimation in Haryana
Get PDFAn attempt has been made to assess the impact of weather variables for district-level wheat yield estimation in Haryana. Fortnightly weather data and trend based yield were used for developing the zonal trendagrometeorological (agromet) models within the framework of multiple linear regression and discriminant function analyses. The district level wheat yield forecasts, percent deviations from the real time wheat yield (s) and root mean square error(s) at zonal level show a preference of using discriminant/weather scores as regressors in almost all the considered districts of the state. Zonal trend-agromet models provided considerable improvement in district-level wheat yield prediction moreover the yield estimates may be obtained 4-5 weeks in advance of the harvest time. The estimated yield(s) from the selected zonal models showed good agreement with State Department of Agriculture (DOA) wheat yields by showing less than 5 percent deviations in 9 districts and 6-11 percent deviations in the remaining 9 districts under consideration
Influence of nitrogen on guttation fluid and development of bacterial blight of rice
Get PDFBacterial blight disease of rice is major constraint in rice cultivation. This disease is influenced by prevailing weather conditions, rice physiology and nutrition. The experiment was carried out to assess the role of nitrogenous fertilizer on development of bacterial blight disease of rice and Xanthomonas oryzae pv. oryzae density in guttation fluid. Disease development increased with increase in nitrogen fertilizer, among four doses of nitrogen tested (control, 36, 54,72 kg urea/acre), disease severity was maximum in the plot supplied with highest dose of nitrogen i.e. 72 kg urea/acre at both seedling (19.3%) and tillering stage (32.4%) at 30 days after inoculation. The highest population density of X. oryzae pv. oryzae (6.02 log cfu/ml) was also recovered in guttation fluid collected from X.oryzaepv. oryzae inoculated plants at tillering stage, from the plots supplied with highest dose (72 kg urea/acre) of nitrogen.Scanning electron microscopic studies also revealed intense colonization by X. oryzae pv. oryzae in rice leaves supplied with high nitrogen dose
Effect of water regimes on seed quality parameters of rice (Oryza sativa L.) grown under aerobic and wetland conditions
Get PDFA field experiment was conducted to know the effect of water regimes on seed quality parameters of rice and the was conducted by using factorial randomized complete block design, with two genotypes (BI-33 and Jaya) and two planting methods (Aerobic and Wetland). The resultants seeds were taken to conduct laboratory experiments pertaining to seed quality attributes and the studies revealed that the BI-33 under aerobic condition had shown a significantly higher seed quality with respect to germination (99%), mean seedling length (26.75cm), seedling dry weight (10.42 mg), SVI-I (2648) and SVI-II (1032) , highest total dehydrogenase activity (0.53 OD @ 480nm), highest amylase activity (14.67 %), highest total soluble protein content (7.15%) and recorded less EC (70.95 ?Sm-1/ppm). The experimental results revealed that both the genotypes and method of planting contribute for seed quality of the genotypes which were grown under different water regimes. The genotype grown under aerobic condition was better in seed quality parameters compared to wetland condition