Journal of Applied and Natural Science
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    Pathogenic variability in Exserohilum turcicum and identification of resistant sources to turcicum leaf blight of maize (Zea mays L.)

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    Turcicum leaf blight of maize incited by Exserohilum turcicum (Pass.) Leonard and Suggs is the major limiting factor of maize production in temperate agro-ecologies. Disease management through host plant resistance is the most effective strategy. In the present study among 26 maize genotypes which were initially screened for resistance against E. turcicum under field conditions, 8 genotypes viz., PS 39, CML 451, CML 470, CML 472, VL 1030, VL 1018140, VL1018527 and SMI178-1 were found resistant when screened against twelve isolates of E. turcicum under artificial epiphytotic conditions. Eight genotypes viz., PS45, CML165, CML459, VL1249, VL0536, SMC-5, SMC-3 and KDL 211 were found moderately resistant with disease grade ranged from 2.1-2.5. These maize genotypes possess resistance to turcicum leaf blight can be used successfully in developing high yielding early maturing varieties for high altitude temperate agro-ecologies. The fungus E. turcicum is highly variable in nature. Variability studies on pathogenicity were conducted on twelve isolates of E. turcicum on eleven putative differential maize lines. During the present study a wide pathogenic variation was observed among the twelve isolates of E. turcicum. Cluster analysis on the basis of similarity or dissimilarity in reaction types exhibited by the differential hosts, clustered the isolates into 6 pathogenic groups. The isolates belonged to higher altitudes (Kti 10, Kti11, Kti5) were found to be more aggressive as compared to the isolates of low altitude areas

    Effect of growth regulators and stages of spray on seed yield and seed quality parameters of ridge gourd [Luffa acutangula (Roxb) L.]

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    The experiment was undertaken to study the influence of different growth regulators on seed yield and seed quality parameters in ridge gourd [Luffa acutangula (Roxb) L.]. There were 30 treatment combinations comprising of two factors; plant growth stage as main plot treatment, and plant growth regulators as sub plot treatment embedded in a split plot design. The results indicated that significantly the highest average fruit weight (32.76 g), matured fruit yield per plant (65.84 g), 100 seed weight (13.41 g) and seed yield per plant (12.79 g) was recorded with spraying of 500 ppm ethereal, whereas significantly the maximum fruit length (20.89 cm) and fruit diameter (5.15 cm) with 25 ppm GA3. Significantly the highest seed germination (69.22 %) was recorded with spraying of 250 ppm ethereal, while application of NAA at 50 ppm recorded significantly the highest vigour index I (2737.85) and vigour index II (5029.33). Spraying of PGR at two to four leaf stage recorded the higher fruit length (20.19 cm), fruit yield per plant (47.49 g), 100 seed weight (13.18 g) and seed yield per plant (11.98 g) as well as highest germination (60.90%), vigour index I (2460.80) and vigour index II (4377.66). It is concluded that spraying of 500 ppm etherel at two to four leaf stage increased the higher fruit length, fruit yield per plant, 100 seed weight and seed yield per plant as well as highest germination, vigour index I and vigour index II

    Effect of seed polymer coating with micronutrients and foliar spray on seed quality parameters of resultant seed in chickpea (Cicer arietinum L.)

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    A laboratory experiment was carried out in the Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur to study the effect of seed polymer coating with micronutrients and foliar spray on the resultant seed quality parameters of chickpea. Among the seventeen different treatments, resultant chickpea seeds obtained from treatment polymer coated seeds (each@ 6 ml/kg of seed) along with the combination of micronutrients viz.,ZnSO4 + Boron + Ammonium molybdate + FeSO4 ( each @ 2 g/kg of seed ) and two foliar sprays (0.5 % + 0.2 % + 0.1% + 0.5 %, respectively, except ZnSO4 and FeSO4 in EDTA form) at an interval of 10 days during flowering stage (50 and 60 days after sowing) recorded significantly highest hundred seed weight (25.9 g), germination (97.00 %), speed of germination (18.50), shoot length (8.43 cm), root length (19.80 cm), seedling dry weight (43.30 mg) and seedling vigour index (2738) compared to all other treatments and control (23.6 g, 92.05 %, 13.74, 5.48 cm, 16.30 cm, 34.00 mg and 2004, respectively) and hence above treatment combinations can be used in order to produce good quality seeds

    Prediction of storability of organically produced paddy seeds through natural and accelerated ageing techniques

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    The present study was conducted to know the storage potential of organically produced paddy seeds in the Department of Seed Science and Technology, University of Agricultural Sciences, Raichur. The seed lot were divided into two parts, one part was stored in cloth bag for a period of 12 months under ambient conditions. At thesame time another set of seeds were subjected to accelerated ageing at 42 + 10C temperature and 90 per cent relative humidity (RH) for a period of 0-12 days. Among the ageing methods, artificially aged seeds showed drastic decreases in seed quality as compared to natural ageing. Among the treatments T9 (37.5 % FYM + 37.5 % vermicompost + 25 % neem cake + foliar spray of panchagavya on 30, 60, 90 and 120 DAT) recorded significantly highest seed quality parameters viz., seed germination (97.81 %), seedling length (29.42 cm) and SVI (2878) at initial stage in both the method of aging and at the end of storage period; seed germination (71.23 and 87.33 %), seedling length (19.66 and 27.00 cm) and SVI (1400 and 2358) in accelerated ageing (AA) and natural ageing (NA) respectively, whereas, lowest in control (Inorganic treatment). The seed quality parameters of four days of AA were similar to that of six months of NA. Hence, storability of organically produced paddy seeds were better as compared to inorganic seeds and it can be predicted that four days of AA is equal to six months of NA. The information generated will be useful in retention or disposal of a particular variety or seed lot

    Bioefficacy of Imidacloprid 350 SC against sucking insect-pests in chilli (Capsicum annum L.)

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    A field experiment was conducted in a RBD at Horticulture farm, Rajasthan College of Agriculture, Udaipur to evaluate the bioefficacy of Imidacloprid 350 SC at 100, 125 and 150 ml/ha against sucking pests of chilli during Kharif, 2013 and 2014. The highest reduction in the population of aphid, jassids and thrips in chilli was recorded in case of two spray of Imidacloprid 350 SC at 150 ml/ha and also recorded highest marketable yield of 161.25 and 164.88 q/ha during 2013 and 2014, respectively. It was found at par to Imidacloprid 350 SC at 125 ml/ha

    Irrigation application efficiency and uniformity of water distribution using multi-outlet pipe and resource conservation technologies

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    Irrigation experiments were conducted during November to April under wheat crop in the winter season of 2012-13 and 2013-14 in the farmer’s field at Galibkhedi village located in Karnal District, Haryana State, India. In the study, collapsible multi-outlet pipe (MOP) along with single outlets pipe (SOP) was tested in farmer’s field under wheat cultivation. Irrigation was carried out in five treatments including tillage (T) with SOP and MOP; zero-tillage (ZT) with SOP and MOP, and furrow irrigation with raised bed (FIRB). Iso-time profile of waterfront spreading and advance indicated that irrigation water distribution was uniform under the plot irrigated using MOP as compared to plot irrigated using SOP. In addition, water distribution was uniform under zero tilled plots as compared to tilled plot. Results implied that MOP has several advantages over SOP in terms of application efficiency (AE) and uniformity of water distribution. Average application efficiency for the first study year was found to be in the order of ZT-MOP (82.41%) > FIRB (76.79%) > ZT-SOP (75.25%) > T-MOP (74.85%) > T-SOP (69.79%). Average application efficiency for the second study year was found to be in the same order as first year with some deviation in values. In the second year values of mean application efficiencies were ZT-MOP (82.58%) > FIRB (77.13%) > ZT-SOP (73.04%) > T-MOP (69.65%) > T-SOP (66.13%). Overall, this study concludes that irrigation under wheat crop using collapsible multi-outlet pipe (MOP) with zero tillage practices is a suitable option for surface irrigation that accomplishes uniform distribution of water with higher application efficiency

    Growth and productivity of Tectona grandis Linn. f. in plantations and farmlands in coastal zone of Karnataka (India)

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    The present study was conducted to understand the growth performance and productivity potential of Tectona grandis grown in plantation as well as in the farmlands at coastal zone of Karnataka. The growth of teak tree grown in farmland was significantly higher than tree grown in pure plantation. For instance, the overall height(11.95 m), diameter (23.69 cm), clear bole height (6.20 m), tree volume (0.61 m3) and timber volume (0.33 m3) was higher in farmland than trees grown in pure plantation (11.60 m, 20.33 cm, 4.90 m, 0.42 m3 and 0.18 m3, respectively). However, crown parameters did not show significant difference among trees grown in pure plantations and farmlands. There was a strong influence of age on growth of teak trees grown in both farmland and pure plantation. For instance, tree height (14.72 m) and diameter (30.52 cm) of higher age class (A4: 21-25 years) was maximum as compared lower age class (A1: 5-10 years) with values of 8.29 m and 17.14 cm, respectively. Similar trend was also recorded for volume, clear bole height, crown height, crown length and crown diameter in teak. It was concluded that teak grown in farmland may produce higher growth and volume as compared to pure plantation

    Effect of elevated CO2 and temperature on growth parameters of pea (Pisum sativum L.) crop

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    Global warming is predicted to have negative effect on plant growth due to the damaging effect of high temperature on plant development. The field experiment was conducted during 2014-15 to study effect of elevated CO2 and temperature on growth parameters of pea (Pisum sativum L.) crop in order to check the effect of climatechange on vegetable crops. Effect was studied under four conditions i.e. Open Top Chambers, T1: OTC - elevated CO2550±10 ppm; T2: OTC -elevated CO2 550±10 ppm and temperature 1°C; T3: OTC - ambient CO2 and temperature (reference) and T4: natural condition. Maximum plant height at 50 % flowering was recorded in T1 (84.29cm) at elevated CO2 which differed significantly with T2 (79.47cm) at elevated CO2 and temperature, T3 (73.60cm) at ambient CO2 and temperature and natural condition (70.73cm). Minimum days to 50 per cent flowering were recorded in plants growing under T2 (68.56 days). Maximum pollen viability was recorded in T1 (77.42%) followed by T3 (76.36%), T4 (74.65%) and T2 (69.97%). Internode length of plants grown under T1 was maximum (7.01cm) followed by T2 (6.68cm), T3 (6.00cm) and T4 (5.05cm). Elevated temperature and CO2 had significant effects on growth and development in vegetables. Overall, growth parameters of pea crop were affected positively by elevated CO2 whereas under interaction effect of elevated CO2 and temperature these positive effects of CO2 were offset by elevated temperature effect and hampered the growth of pea crop which interns can affect the yield and quality of crop under changing climate scenario

    Growth, fruit set and yield of Santa Rosa plum as affected by nitrogen and boron under rainfed conditions of Kashmir Valley

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    Present experiment was carried out in a seven year old private plum orchard near SKUAST-Kashmir, Shalimar Campus, Srinagar during 2012 and 2013, to examine the response to nitrogenous fertilizer and boron on growth, fruit set and yield of plum var. Santa Rosa. Urea @ 500 g (N1), CaNO3 @ 1450 g (N2), N1 + 50 g boron (N3) and N2 + 50 g boron (N4) were applied at T1 = Full dose in spring, T2 = Full dose after harvest and T3 = 3/4 dose in spring and 1/4 dose after harvest. Observations were recorded on parameters viz. incremental tree girth, shoot extension growth, plant height, plant spread, leaf area, fruit set, fruit drop, yield, yield efficiency. All the growth parameters were high in treatment combination N1T1. Maximum fruit set (19.68 % and 20.90 %) was recorded in N3T2, yield (20.60 kg/tree and 22.88 kg/tree) was recorded in N4T2, yield efficiency (0.45 and 0.46 kg/cm2) was recorded in N4T2 and minimum fruit drop (4.25 % and 4.55 %) were recorded under the treatment combination N4T2 and N4T3, respectively in the both the years. Both sources of nitrogen and boron can be considered as best fertilizer in plum orchards for improving the growth, fruit set, fruit yield, yield efficiency

    In vivo evaluation of ziram induced acute toxicity on pathomorphology of broiler chicken

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    Fungicides are usually used in agriculture and often find their way in poultry feed. Therefore, a study was undertaken to study the in vivo effect of one such fungicide (ziram) induced intoxication on pathomorphology of broiler chicken. After 2 weeks of age the birds were given fungicide (Ziram) in feed as a single oral dose of 100 mg/kg body weight. Out of 10 birds, 3 died due to ziram intoxication (30% mortality rate). Birds that died rapidly showed pronounced neurological signs like convulsions. The carcasses of ziram intoxicated birds appeared dehydrated and their mucous membrane was pale in colour. Marked vascular congestion was observed in brain on gross examination. The livers showed congestion and haemorrhages with necrotic foci. Kidneys and lungs had ecchymotic haemorrhages and heart revealed gelatinization of pericardium, distention and pericarditis. Atrophy of bursa of Fabricius and thymus; hypertrophy of thyroid was found. Histopathological examination revealed neuronal degeneration and necrosis associated with mild gliosis in brain. Lungs, pericardium and epicardium had severe congestion and there was degeneration with separation of myofibers. Glomeruli were congested and frequently revealed hypercellularity. There were sinusoidal congestion and varying degrees of hepatocellular degeneration. Bursa revealed mild depletion of lymphoid cells in few lobules while as thymus showed hypoplasia with depletion of lymphocytes. Thyroid had mild mononuclear cell infiltration and caecum showed marked necrosis and denudation of the mucosa. In conclusion, the depletion of lymphoid tissue from lymphoid organs was suggestive of immunosuppressive and immunomodulatory effects of ziram toxicity in broiler chicken

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