Journal of Applied and Natural Science
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    Studies on development and storage stability of dehydrated pumpkin based instant soup mix

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    The study was carried out to develop and standardize Instant Soup Mix (ISM) from dehydrated pumpkin powder and to evaluate nutritional (moisture, sugars, protein, ?-carotene, fat, fibre and water activity) and sensory qualities (colour, texture, flavour and overall acceptability) for determining its shelf-life during a period of six month. It was packed in aluminium laminated pouches and was analyzed periodically for changes in quality. Among various recipes optimized for the development of soup mix, soup mix containing 20g pumpkin powder, 5g moong dhal, 15g tomato powder, 11.4g spices (salt and black pepper) and condiments (onion, garlic and ginger powder), 2g dried pea, 2g dried spinach, and 2g dried carrot was selected as base recipe for addition of different starch source viz. rice, corn and potato @ 10 g. From the nutritional analysis, it was observed that corn based Instant Soup Mix had higher ?-carotene (7.01 mg/100 g) and protein (12.65 %) content, while fibre (2.09 %) was higher in soup mix containing rice starch. It was observed that on the basis of sensory evaluation corn based soup mix was more acceptable. Therefore, results of nutrition and sensory evaluation indicated that a good quality ISM can be prepared by using corn starch. During the six month of storage, there was about 5.49 per cent increase in moisture, 3.16 and 5.27 per cent decrease in protein and ?-carotene, respectively, along with slight losses in total sugars, fat and sensory quality. Further, the product was stable for 6 months under ambient condition

    Osmolytes: Proline metabolism in plants as sensors of abiotic stress

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    Proline accumulation occurs in a large range of plant species in retaliation to the numerous abiotic stresses. An exclusive research pattern suggests there is a pragmatic relation between proline accumulation and plant stress tolerance. In this review, we will discuss the metabolism of proline accumulation and its role in stress tolerance in plants. Pertaining to the literature cited clearly indicates that not only does it acts as an osmolyte, it also plays important roles during stress as a metal chelator and an antioxidative defence molecule. Moreover, when applied exogenously at low concentrations, proline enhanced stress tolerance in plants. However, some reports point out adverse effects of proline when applied at higher doses. Role of proline gene in seed germination, flowering and other developmental programmes; thus creation of transgene overexpressing this gene would provide better and robust plants. In this context this review gives a detailed account of different proline gene over-expressed in all the trans-genic crops so far

    Effect of growth and yield parameters on Indian-mustard genotypes under varying environmental conditions in western Haryana

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    An experiment was conducted in Rabi season of year 2014-15 at Research Farm, Department of Agril. Meteorology, CCSHAU Hisar, Haryana and field area was adjacent to agrometeorological observatory at 290 10\u27 N latitude, 750 46\u27 E longitude and altitude of 215.2 m with Split Plot Design as main plot treatments consisted of three date of sowing viz.26thOctober, 5thNovember, and 15th November and sub-plots consisted of three varieties (Kranti, RH 406 and RH 0749) with four replications. Various growth and yield parameters such as plant height, LAI, dry matter accumulation, partitioning and yield attributes were higher in 26thOctober sown crop as compared to 5th and 15th November at all the growth intervals. The crop sown on 26th October (1870.3 kg/ha) produced highest seed yield as compared to 5th (1525.5 kg/ha) and 15th November (1099.8 kg/ha). Among varieties, RH0749 recorded highest seed yield because LAI, biomass accumulates were performed better as compared to RH 406 and Kranti. There was significant interaction between growing environment and varieties with respect to growth and yield parameters. From the above study it was concluded that normal or early sowing of Indian mustard may be practisized for achieving higher seed yield and improved growth and yield attributes in western Haryana conditions

    Economic analysis of trout feed production in Jammu and Kashmir, India

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    The Present study is an attempt to understand the economics of trout feed production in the state of Jammu and Kashmir. Trout feed production is capital intensive business which requires high initial capital investment. The results revealed that major fixed investment required in trout feed production was feed mill itself which accounted about 71.44 percent of the total investment. The cost and return analysis showed that the variable cost accounts 59.16 percent whereas fixed cost accounted 40.84 percent of the total cost respectively. Among the variable cost raw material was found out to be single most important factor which accounted about 56.37 percent of the total cost which was about 95.28 percent of the total variable cost. The average cost of production of trout feed was Rs.84.33/kg which ranged from Rs.78.45/kg in Kokarnag trout feed mill to Rs.90.2/kg in Manasbal trout feed mill but government has fixed selling price at Rs.73/Kg for the feed to maintain reasonable price level for private trout farmers. The availability and high price of raw material were found to be major constraints faced by feed producers. Economics analysis revealed that both the feed mills are operating at suboptimal level and there is need to utilize the feed mill to its full potential and export the surplus production to neighbouring state of Himachal Pradesh and other Himalayan states like Sikkim and Arunachal which will help the state fisheries department to generate extra income which can be used in other developmental activities

    Impact assessment of frontline demonstrations on green gram: Experience from rainfed condition of Rajasthan

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    Pulses being rich in quality protein, minerals and vitamins are inseparable ingredients of diet of majority of Indian population. Despite high nutritive value of pulses and their role in sustainable agriculture desired growth rate in production could not be witnessed. The domestic production of pulses is consistently below the targets and actualdomestic requirements are also higher, due to these pulses are being imported. The Krishi Vigyan Kendra Pali has carried out frontline demonstrations on green gram covering an area of 26.5 ha of farmers’ field to exhibit latest production technologies and compared it with farmer’s practice. The study in total 40 frontline demonstrations were conducted on farmers’ fields in villages viz., Kishanagar, Bedkallan, Boyal, Kushalpura and Balara of Pali district of Rajasthan state during 2014, 2015 and 2016, to demonstrate production potential and economic benefit of improved technologies comprising sowing method, nutrient management and chemical weed control and adoption of whole package of practices for the crop. After sowing of seed application of weedicide Pendimethalin (within two days after sowing) at 1.0 kg/ha in 500 liters of water used for effective control of the weeds during kharif season in rainfed condition. The findings of the study revealed that the demonstrated technology recorded a mean yield of 982 kg/ha which was 35.5% higher than obtained with farmers’ practice (755 kg/ha). Higher mean net income of Rs. 46030/ha with a Benefit: Cost ratio of 4.3 was obtained with improved technologies in comparison to farmers’ practices (Rs. 38775/ha). The frontline demonstrations conducted on green gram at the farmers’ field revealed that the adoption of improved technologies significantly increased the yield as well as yield attributing traits of crop and also the net returns higher than the farmers’ practices. So, there is a need to disseminate the improved technologies among the farmers with effective extension methods like training and demonstrations. The farmers’ should be encouraged to adopt the recommended package of practices realizing for higher returns

    Occurrence of functional single-lobed ovary in Cirrhinus mrigala (Hamilton,1822) brood fish from Assam, India

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    Occurrence of abnormally developed gonads in fish is rather more uncommon in freshwater fish than marine fish. It is therefore worthwhile to disseminate the information of occurrence of a single-lobed ovary in Cirrhinus mrigala brood fish as an abnormal phenomenon. During April 2011 to March 2013, around 500 brood fish samples of Indian major carps (Labeo rohita, Catla and C. mrigala) were collected from different government and private fish farms of Assam and Tripura located in the Northeast of India. Among them, one C. mrigala specimen measuring 41 cm in total length and 640 g in weight collected from Ulubari fish seed farm of Guwahati, Assam during June, 2012 was found to have a single-lobed ovary instead of the normal bilobed structure. The ovary occupied the entire body cavity and the majority of ova were round and translucent. The ovary contributed substantially to the total body weight with gonado-somatic index of 32.81 which was the highest among all the mrigal specimens examined. The study indicates possibility of artificially inducing development of single-lobed ovary in C. mrigala for achieving possible higher spawn outputs in induced breeding of the cultivable species

    Combining ability and heterosis analysis for fibre yield and quality parameters in roselle (Hibiscus sabdariffa L.)

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    Roselle (Hibiscus sabdariffa L.) is second important bast fibre crop after jute in India. With an aim to ex-ploit non-additive genetic variance present experiment was designed to identify good general combining parents and specific cross combination for fibre yield and fibre quality parameters (fibre fineness, fibre tenacity) in roselle. A total of 11 parents were crossed in complete diallel fashion which resulted 55 F1, 55 RF1 (reciprocal F1). Parents, F1s and RF1s were grown in randomized block design. Analysis of variance revealed significant differences (P< 0.01, P<0.05) among the parents and their hybrids. The parents AMV 1, AMV 5, GR 27 and AHS 160 were identified as good combiners since they recorded significant general combining ability (GCA) effects for fibre yield and quality parameters. Further, For fibre yield only three crosses (AMV 1 × AMV 4, AMV 1 × GR 27, HS 4288 × JRR 07) showed significant specific combining ability (SCA) effects from them hybrid AMV 1 × GR 27 (fibre yield=27.37g/ plant) exhibited positively significant best parent (Non bris 4, Mean fibre yield=21.16g/plant) heterosis (29.35%). Similarly, for fibre tenacity, hybrid GR 27 × JRR 07 (fibre tenacity=23.47g/tex) exhibited positively significant best parent (HS 4288; fibre tenacity=20.35g/tex) heterosis (15.30%)

    Cell membrane stability- an important criterion for selection of heat tolerant genotypes in wheat (Triticum aestivum L.)

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    Cell membrane stability, grain filling rate, grain filling duration, canopy temperature and grain yield were used to evaluate performance of 100 diverse bread wheat (Triticum aestivum L.) genotypes under timely sown and late sown heat stress conditions for two cropping season. The genotypes differed significantly for all the traits show-ing considerable variation for improvement of characters. The genotypes WH1165 had significant high grain yield (14.6* g and 11.4g) and (11.3* g and 11.4* g) followed by cell membrane stability under timely sown and heat stress conditions, respectively indicating potential tolerance against heat stress. Correlation coefficients revealed that cell membrane stability (0.451**) and (0.639**) in timely sown and in late sown conditions, respectively were the most important trait followed by grain filling rate (0.882** and 0.744**) under timely sown and late sown conditions respec-tively. Results revealed that bread wheat genotypes which had high value of cell membrane stability had high grain yield showed potential photorespiration and high grain filling rate under heat stress condition. Twenty two genotypes WH1021, WH1155, VL803, WH787, NW1014, Raj3765, HD1869, 2042, WH1124, HD2285, WH1133, HUW234, 4066, Sonak, UP2425, UP2473, PBW503, PBW373, PBW533, SGP13, HD2643 and WH789 were identified as heat tolerant genotypes based on their relative performance in yield components, grain yield and heat susceptibility indi-ces. These genotypes were found to be ideal candidates to be used in developing heat tolerant wheat varieties. Canopy temperature, membrane thermostability and grain filling rate have also shown strong correlation with grain yield. Because of this association, these traits constitute the best available ‘tool’ for genetic improvement of wheat suitable for cultivation under heat stressed environments. Thus, these could be used as indirect selection criteria for developing heat tolerant wheat genotypes that would provide sufficient yields to meet the ever increasing wheat demand

    Biointensive integrated management of Lipaphis erysimi Kalt. (Homoptera: Aphididae) in Brassica spp.

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    Field experiment was conducted at Regional Research station, Samargopalpur, Rohtak (Haryana) during Rabi season of the year 2015-2016 to evaluate bioefficacy of various treatments against mustard aphid, Lipaphis-erysimi on Indian mustard. Treatments were: T1–Verticillium lecanii @ 108 CS/ml, T2–Beauveria bassiana @ 108 CS/ml, T3 - Neem seed kernel extract @ 5%, T4 - Neem seed methanol extract @ 5%, T5 - V. lecanii @ 108 CS/ml + Clipping of infested twigs, T6 - B. bassiana @ 108 CS/ml + Clipping of infested twigs, T7 - NSKE @ 5% + Clipping of infested twigs, T8 - V. lecanii @ 108 CS/ml + NSKE @ 5%, T9 - B. bassiana @ 108 CS/ml + NSKE @ 5%, T10 - Dime-thoate 30EC @ 250 ml/acre. Dimethoate was found to be most effective in reducing the aphid population (95.03 %) followed by V. lecanii @ 108 CS/ml + NSKE @ 5% (88.52 %), NSKE @ 5% + Clipping of infested twigs (87.77 %) and B. bassiana @ 108 CS/ml + NSKE @5% (86.91 %) after ten days of spray. The highest seed yield was recorded in treatment dimethoate 30EC (1702 kg/ha) followed by V.lecanii @ 108 CS/ml +NSKE @ 5% (1635 kg/ha), NSKE @ 5% + Clipping of infested twigs (1626 kg/ha) and B.bassiana @ 108 CS/ml + NSKE @ 5% (1617 kg/ha). Dimethoate was found to be highly cost effective with highest cost benefit ratio (1:14.92) followed by NSKE @ 5% + clipping of infested twigs (1:13.81) and NSKE @ 5% (1:11.41)

    Evaluation of bupirimate against rose powdery mildew

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    Bupirimate 25% Emulsifiable concentrate (EC) was evaluated for efficacy on Sphaerotheca pannosa, the causal agent of rose powdery mildew in vivo. In this experiment Bupirimate 25% EC 6 ml/L and 4 ml/L effectively reduced the powdery mildew infection over rest of the treatments and improved the flower yield. Moreover, application of Bupirimate 25% EC at the doses of 2, 4 and 6 ml/L and even at higher dose 8 ml/L did not show any phyto-toxic symptoms on rose plant. Thus, Bupirimate 25% EC may be considered as compared to other fungicides

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