Journal of Applied and Natural Science
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Soil test crop response based Integrated plant nutrition system for desired yield target of preseasonal sugarcane ratoon on Inceptisols
Get PDFStudies on Soil Test Crop Response based Integrated Plant Nutrition System (STCR - IPNS) were conducted adopting the Inductive cum Targeted yield model onInceptisols (VerticHaplustepts) in Rahuri, District Ahemadnagar, Maharashtra, India in order to develop fertilizer prescriptions through IPNS for the desired yield targets of preseasonal sugarcane ratoon. The field experiments were carried out with maize as gradient crop for plant cane and after harvest of plant cane, pre-seasonal sugarcane ratoon as test crop. Using the data on yield, initial soil test values on available nitrogen (N), phosphorus (P), potassium (K), doses of fertilizers and farmyard manure (FYM) applied and NPK uptake, the basic parameters viz., nutrient requirement, contribution from soil, fertilizers and FYM were computed. It was found that 1.56 kg N, 0.58 kg P and 1.04 kg K were required for producing one tonnemillable cane of preseasonal ratoon sugarcane. The per cent contributions of N, P and K from soil and FYM for preseasonal sugarcane ratoon were 37.65, 85.88 and 19.82 per cent and 11.83, 10.88 and 12.24 per cent, respectively. Making use of these basic parameters, fertilizer prescription equations were developed for pre-seasonal sugarcane ratoon (var. C0-94012) and an estimate of fertilizer doses formulated for a range of soil test values and desired yield targets under NPK alone and IPNS (NPK plus FYM)
Genetic analysis for various yield components and gluten content in bread wheat (Triticum aestivum L.)
Get PDFGenetic analysis was carried out in 55 genotypes (10 parents and 45 F1s) through diallel mating design excluding reciprocals in bread wheat (Triticum aestivum L.). Analysis of variance showed wide range of variability among the breeding material for all the traits under study. The highest value of phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) were recorded for grain yield (PCV= 9.07 and GCV= 8.08). Highest heritability with genetic advance was recorded for grain yield (h2=10.60 and GA=14.84), therefore selection will be effective based on grain yield for further study. Grains per spike (gr = 0.77 and pr = 0.67) and spikelets per spike (gr= 0.63 and pr = 0.52) were found significantly correlated (at <1 % level of significance) with grain yield whereas gluten content showed nonsignificant but positive correlation with grain yield at both genotypic as well as phenotypic level. Similarly, path coefficient analysis estimates for gluten content (g= 0.08 and p= 0.03) and grains per spike (g=0.36 and p=0.23) showed high positive direct effects on grain yield therefore these traits may be used as an index for selection to high yield in bread wheat genotypes
Integration of biocontrol agents with fungicide, weedicide and plant growth regulator for management of stem and root rot of jute
Get PDFCombination of chemical fungicides (viz., Carbendazim 50 WP and Tebuconazole 250 EC) and biocontrol agents (viz., Pseudomonas fluorescens Psfl1, P. striata Pst1, Azotobacter chrococcum Azbc3, Bradyrhizobium japonicum Brj4, Trichoderma aureoviridae S12, T. harzianum JTV2, T. virens JPG1, Aspergillus niger AN15 strains respectively either singly or in consortium) were used to counteract Macrophomina phaseolina, the causal organism of stem and root rot of jute. In addition, suitable plant growth regulator viz., Indole-3-acetic acid (100-1.0 µg/ppm) and herbicide Quizalofop ethyl 5 % EC were used to augment the activity of Trichoderma. T. aureoviridae strain S12 was found to be the best among the eight isolates screened for tolerance against the two fungicides and herbicide at a concentration of 10000 - 500 µg respectively as well as against M. phaseolina (Inhibition=72.33 %) in-vitro. This strain showed best compatibility with other strains and highest tolerance to fungicide i.e., Carbendazim 50 % (up to 500 ?g). Highest number (13.7×106) of active spores was recorded at a concentration of 25 ppm of IAA under in-vitro condition. S12 recorded a biocontrol efficiency of 61.8 % against stem rot of jute along with significant plant growth promotion and fibre production. Plant biomass also increased up to 7.5-12.1 % and fibre production 37.0-39.9 % with fungal and bacterial consortium + carbendazim seed dressing and soil drenching. These biocontrol fungi and PGPR consortium with high tolerance to fungicide, weedicide and plant growth regulator up to certain extent may be potentially exploited in IDM which may be a low cost technology in jute and allied fibre crops
Morphological characterization of forage sorghum genotypes for its various DUS traits
Get PDFThe present investigation was conducted to characterize 20 genotypes of sorghum {Sorghum bicolor (L.) moench} on the basis of 33 morphological characters provided by Protection of Plant Variety & Farmer’s Right Act (PPV&FRA) for Distinctiveness Uniformity and Stability (DUS) testing in sorghum. Experimental results revealed that maximum variation was found on the basis of glume colour among the genotypes i.e. G 46, HC 308, HJ 513 had green white, IS 3237, SSG 9, HC 171 had yellow white, SSG 59-3, COFS 29 had grayed purple, S 437-1, SGL-87, S 540-S, SSG (PSSG) had grayed yellow and remaining seven genotypes had grayed orange glume colour. The studied traits showed five genotypes had distinct state of expression. Genotype S-540 showed very high plant height upto the base of flag leaf, HC 136 had compact panicle density at maturity, COFS 29 had very long glume length, SSG 59-3 had distinct expression for days to panicle emergence (50 % of the plants with 50 % of anthesis) and COFS 29 and IS 18551 had short and very long leaf width of blade, respectively. The Principal Component Analysis (PCA) revealed principal Factor (PFI) and Principal Factor (PFII) with maximum variability (64.99 %). Classification of genotypes on the basis of DUS traits provided identification of key characteristics of various genotypes
Biocontrol of toxigenic strain of Aspergillus flavus isolated from the root tubers of safed musli (Chlorophytum borivilianum Sant. F) using its rhizospheric mycoflora
Get PDFMiraculous herb safed musli (Chlorophytum borivilianum Sant. F), family liliaceae, is well recognized for its immense potential as an aphrodisiac. The root tubers of this herbal drug were found to be invested with Aspergillus flavus during field and storage. Therefore, the present study was designed to explore the ability of 26 co-existing rhizospheric mycoflora to inhibit A. flavus invasion and subsequent aflatoxin contamination of safed musli. The interaction of these moulds with highly toxigenic strain (CB55) of A. flavus was evaluated by dual culture method and type of interaction was graded. Most likely antagonistic effects were shown by fifteen (15) moulds, out of which Type ‘C’ interaction was evidenced in the case of six moulds; A. clavatus, A. terreus, Botryotrichum piluliferum, Candida albicans, Cephalosporium acremonium, and Cunninghamella sp. Further, ‘D’ type interaction was displayed by seven moulds which include A. niger, Colletotrichum sp., Drechslera sp., Mucor haemalis, Mycelia sterilia, Rhizopus arrhizus and Stachybotrys atra and ‘E’ type interaction was noted in the case of Trichoderma viride and Trihcothecium roseum. Regarding human health it is critical to use an ecofriendly approach to control the invasion of toxigenic moulds with root tubers of safed musli
Linseed (Linum usitatissimum L.) genetic resources for climate change intervention and its future breeding
Get PDFLinseed or flax (Linum usitatissimum L.), a multiple purpose crop valued for its seed oil, fibre, probiotic and nutraceutical properties, is adapted to different environments and agro-ecologies. Modern breeding techniques using only limited number of selected varieties have resulted in a loss of specific alleles and thus, reduction in total genetic diversity relevant to climate-smart agriculture. However, well-curated collections of landraces, wild linseed accessions and other Linum species exist in the gene banks and are important sources of new alleles. This review is primarily focused on the studies of genetic diversity of linseed species and evaluation related to tolerance to abiotic and biotic stress factors that could be useful for improving linseed through future promising breeding programs in addition to briefly discussing different morphotypes and nutraceutical importance. Wide diversity in linseed germplasm indicates a considerable potential for improving this crop for both agronomic and quality traits required for developing climate-resilience tailored to specific environments. Recent release of the flax genome sequence coupled with wide range of genomic and analytical tools in public domain has furthered understanding of molecular mechanisms for detailed study of the genes underlying flax adaptation to stress and diversity in commercially important accessions. Important climate related traits and their constituent genes are presented and key developments for the future highlighted emphasizing the urgent need to increase the use of genetically diverse germplasm to meet the emerging challenges in agricultural production and to conserve valuable genetic resources for the future
A rapid and reproducible method for isolating genomic DNA from a few crop plants suitable for polymerase chain reaction-based genotyping
Get PDFAs most of the molecular markers in crop molecular breeding programmes are successful based on polymerase chain reaction (PCR), the isolated genomic DNA must be suitable for the same. Though PCR is a robust method and in most of the cases requires only a minute amount of genomic DNA as template, removal of potential PCR-inhibitory factors is quite important. The present work reports the optimization of a rapid genomic DNA isolation method, suitable for PCR-based genotyping of plants. As very minute amount of the genomic DNA isolated in this rapid method was found to be sufficient for PCR, a researcher is capable to go for several hundred independent PCR from single isolation. The method was validated in 4 different crops (wheat, tomato, brinjal and cauliflower) using different PCR-based molecular markers. In case of wheat, genomic DNA isolated in this method was found to be suitable PCR using the specific marker for the detection of the Lr34 gene. For tomato, genomic DNA isolated in this method was successfully used with the molecular markers for the detection of resistance alleles for yellow leaf curl disease and root knot disease. In case of brinjal, the isolated genomic DNA was found to be suitable for simple sequence repeat (SSR) marker assay. In a similar way, genomic DNA isolated in this method from cauliflower leaves was observed to be suitable for amplifying a gene of ~1.5 kb length. Thus, this method will be quite helpful to expedite marker assisted selection of plants in plant molecular breeding programmes
Nitrogen release kinetics of organic nutrient sources in two benchmark soils of Indo-Gangetic plains
Get PDFAn understanding of the mineralization process of organic amendments in soil is required to synchronize N release with crop demand and protect the environment from excess N accumulation. Therefore, we conducted a laboratory incubation experiment to assess nitrogen mineralization potential of crop residues (rice and wheat straw) and organic manures (poultry manure, farmyard manure, cowpea and sesbania) in two benchmark soils (Typic Haplustept and Typic Ustifluvents) of semi-arid region of Punjab, India, varying in textureat field capacity moisture level at a constant temperature of 331°C. Mineralization was faster during first 7 days of incubation in Typic Haplustept and upto 14 days in Typic Ustifluvents which subsequently declined over time. In both soils, net N mineralization continued to increase with increasing period of incubation (expect with crop residues) and was significantly higher in Typic Ustifluvents (54-231µg g-1) than Typic Haplustept (33-203 µg g-1). Compared to unamended soils, percent N mineralized was highest is sesbania (35-40 %) followed by cowpea (32-37 %) and least in wheat (10-11 %) after 42 days of incubation. Thus, sesbania and cowpea may preferably be used to meetthe large N demand during early period of plant growth. Further, mineralization rate constants (k) also indicated that availability of mineral N was significantly higher with application of organic amendments than unamended control treatments in both soils. Therefore, it may be concluded that considerable economy in the use of inorganic N fertilizer can be employed if N mineralization potential of organic inputs is taken into consideration
Effect of plant growth promoting rhizobacteria (PGPR) and IBA treatments on rooting in cuttings of apple (Malus × domestica Borkh.) clonal rootstock Merton 793
Get PDFThe preliminary studies on the effect of different strains of plant growth promoting rhizobacteria (PGPR) alone and in combination with IBA at 1000 ppm on rooting in cuttings of apple clonal rootstock Merton 793 were carried out during 2012-13. The PGPR strains (RG (1)3 – Bacillus sp.), B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. alone failed to induce rooting response in cuttings of apple clonal rootstock Merton 793. The results revealed that IBA 2500 ppm recorded the maximum rooting (65 %), number of primary roots (5.00), length (28.43 cm) and diameter (3.25 mm) of primary roots, fresh (3.67 g) and dry weight (2.59 g) of roots, length of main shoot (134.14cm), diameter of main shoot (8.18 mm), fresh (30.40 g) and dry weight (22.60 g) of shoots in cuttings of Merton 793. However, the PGPR strains RG (1)3 – Bacillus sp., B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. in combination with IBA 1000 ppm showed improvement in rooting of cuttings to the extent of 10, 15 and 5 per cent rooting, respectively and growth of the rooted plants. IBA at 2500 ppm resulted better rooting and growth of rooted plants. Hence, this treatment is suggested for commercial propagation of apple clonal rootstock Merton 793 through cuttings
Determination of heavy metal pollution index of ground water of village Wallipur in Ludhiana district
Get PDFThe objective of the study was to reveal the seasonal variations in the groundwater quality with respect to heavy metals contamination near Buddha Nullah in Ludhiana district. To get the extent of trace metals contamination, groundwater samples from tube wells were randomly collected from 16 different points on both sides along the course of Buddha Nullah from areas of Bhamian Kalan, Khasi Kalan and Wallipur Kalan villages of Ludhiana district during first fortnight of June (Pre-monsoon), first fortnight of November (Post-monsoon) and first fortnight of January (Winter season) during the year 2013-14. The concentrations of toxic and heavy metals such as metalloids B, As, Pb; alkaline earth metals Mg, Ca; alkali metals Na, K; transition metals Cr, Mn, Fe, Cu, Zn, Cd and nonmetal S were determined. The heavy metal pollution index (HPI) was calculated. Most of the parameters were found within permissible limit of BIS, 2004. The average values of concentration of Ca, Cr, Mn, As and Pb for pre-monsoon season was higher than average concentration of post-monsoon and winter season. Overall HPI calculated based on the mean concentration of the heavy metals was found to be 18.11, 15.32 and 16.10 for pre-monsoon, post-monsoon and winter season, respectively, which was below the critical pollution index value of 100. The study recommended proper treatment to the sewage water which is being discharged into the Buddha Nullah