Journal of Applied and Natural Science
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Heterosis study in Okra [Abelmoschus esculentus (L.) Moench] genotypes for pod yield attributes
Get PDFA study was conducted at Vegetable Research Farm, Department of Horticulture, Institute of Agriculture Sciences, Banaras Hindu University, Varanasi during Spring-Summer and Rainy season of 2012 and 2013 using 12 diverse parental lines of okra and their 66 F1 hybrids (through diallel cross-excluding reciprocals) with the objective to measure the extent of heterosis over better parent and standard commercial check varieties for the purpose of judging the extent up to which heterosis can be exploited in commercial okra breeding. The extent of heterosis for five best crosses over better parent and check (48.32 % to 82.42 % and 7.13 % to 35.66 %, respectively) for yield per hectare suggested the great scope of realizing higher yield in okra through heterosis breeding. Other economic traits also recorded moderate to high level of heterosis over the better parents. The cross combination IC -282280×EC – 329380showed high heterosis over better parent and standard check for pod yield (82.42 % and 35.66 %), number of pods per plant (62.82 % and 48.54 %) and respectively. This particular cross combination eventually resulted the height magnitude of heterobeltiosis and standard heterosis for the most of the desirable growth parameters as well as yield attributing characters which may be taken for further breeding programme
Detection of Aflatoxin B1 through indirect ELISA from fresh grains obtained from three maize growing zones of India
Get PDFAflatoxin B1 (AFB1) is most frequently found in plant substrates, which has shown the highest toxigenic potential. Based on previous studies, the IARC has classified AFB1 as a class 1A human carcinogen. Several impacts on consumers, such as loss of human and animal lives; health care and veterinary care costs; contaminated foods and feeds disposal costs; and investment in research and management of the myco-toxin problem. Fourteen maize seed samples comprising of recommended and local varieties were collected from three maize growing zones (Zone I- Almora, Kullu, Bilaspur, Dhaulakuan, Kangara, Saharanpur, Zone II- Karnal, Ludhiana, Pantnagar, New Delhi and Zone III- Begusarai, Varanasi, Sabour-1 and Sabour 2). In our studies AFB1 toxin range were noticed Zone-I (0.0294- 153.5081 ppb), Zone-II (0.1761- 161.0537 ppb ppb) and Zone-III (3.8366- 53.1256 ppb) collected seed samples.This indicate that ELISA technique could be applied to the monitoring of Aflatoxin contamination in a lot of samples in a cost, accuracy, simplicity and time effective manner
Characterization and categorization of Indian mustard genotypes for agro-morphological traits
Get PDFA total of sixty genotypes and germplasm lines were evaluated and characterized for 14 quantitative and 8 qualitative traits in Brassica juncea. Observations were recorded on the basis of scores given in the DUS descriptor. Majority of accessions were very late in maturity and medium in flowering. No variability was observed for leaf length and leaf width. On the basis of branches, most of the genotypes were classified under intermediate category. Long main shoot length (31), medium number of siliquae on main shoot (46), medium density on main shoot (52), short siliqua length (51), very tall plant height (38), few numbers of seeds per siliqua (33), medium 1000-seed weight (38), medium seed yield per plant (32) and low oil content (40) were observed in most of the genotypes. For qualitative traits, most of the genotype showed dark green leaf color, sparse hairs, dentation of leaf margin lyrate type, open leaf growth habit, yellow petal color, semi-appressedsiliqua angle with main shoot and intermediate siliqua surface texture. Wide (Yellow, Dull grey, Reddish brown, Brown and Black) diversity has been observed for seed color
Effect of nitrogen and foliar spray of potassium nitrate and calcium nitrate on growth and productivity of yellow sarson (Brassica campestris L. var yellow sarson) crop under irrigated condition
Get PDFA field experiment was conducted during rabi season of 2007-’08 and 2008-’09 to study the effect of calcium nitrate {Ca (NO3)2} and potassium nitrate {KNO3} at 50 % flowering stage and soil applied nitrogen {N: (0, 40 and 80kg N/ha)} on growth, productivity and economics ofyellow sarson at farmers field under irrigated old alluvial soil of West Bengal. Highest leaf area index (LAI) values at 40 days of crop age (1.748 and 1.592), dry matter accumulation (1404.3 and 1288.8 gm-2) at 80 days and crop growth rate (C.G.R) (27.87 and 25.68 g m-2 day-1) in between 40 to 60 days were obtained at Nitrogen application at the rate of 80 kg / ha and mixed spray of 0.203 % Ca (NO3)2 + 0.25 % KNO3 respectively. Soil application of 80 kg N/ ha along with foliar spray of 0.203 % Ca (NO3)2 + 0.25 % KNO3 resulted increase in seed yield (1.68 t ha-1) by 12 % over 80 kg N/ ha with only water spray (1.5 t ha-1). Foliar spray of 0.203 % Ca (NO3)2 + 0.25 % KNO3 improved the yield components and seed yield over their sole application. Application of 80 kg N/ha along with 0.203 % Ca (NO3)2 + 0.25 % KNO3 at 50 % flowering stage was found to be the most effective in increasing yellow sarson production in old alluvial soil
Comparative analysis of life tables of Bactrocera tau (Diptera: Tephritidae) collected from different geographical regions of North India
Get PDFThe tomato fruitfly, Bactrocera tau (Walker) (Diptera: Tephritidae) is one of the most important pests of the family Cucurbitaceae. Toinvestigate local adaptation, we measured the variations in life-histories and life-table parameters among populations from five different geographical regions of North India, Ludhiana (262 mt amsl), Solan (1,502 mt amsl), Hisar (215 mt amsl), Pantnagar (344 mt amsl) and Jaach (733 mt amsl). The principal components analysis showed the life-history and life-table parameters of B. tau differed among the five geographical populations. The highest fecundity of 233.20 eggs/female was recorded in the Jaach population and was statistically at par with Hisar (209.21 eggs/female) followed by Solan (202.60 eggs/female), Pantnagar (178.60 eggs/female) and Ludhiana population (105.88 eggs/female). The total developmental period among the five populations of B. tau was the longest for the Pantnagar population (16.20 days) followed by Solan (13.90 days), Hisar (12.60 days), Ludhiana (12.30 days) and Jaach (11.60 days). The true intrinsic rate of natural increase (rm) was 0.120, 0.138, 0.140, 0.116 and 0.153 for the respective geographical regions while the finite rate of increase (?) was 1.13, 1.15, 1.15, 1.12 and 1.16, thus indicating that the fruit fly from Jaach location is more reproductive than the other five geographical regions of North India. Since the study will be useful in knowing the multiplication rate of fruit fly in specific area, accordingly the management practices for this species can be formulated on the bases of these studies. The results thus indicated the geographical variations among different populations of B. tau
Effect of cold stress on boro rice seedlings
Get PDFCold stress at seedling stage is a major constraint in boro rice production. Nine boro rice lines were crossed in diallel fashion excluding reciprocals to obtain 36 crosses. All the 36 crosses along with parents were grown in nursery in three seasons (boro-2014, kharif-2015 and boro-2015). Performance of seedlings for survival per cent, chlorophyll content, relative water content, membrane stability index was recorded just before transplanting in all the three seasons. Scoring for cold tolerance was done in both boro seasons. Gautam showed highest survival rate over three seasons. Among crosses, IR 64 x Krishna Hamsa showed highest survival (84%) in boro-2014, MTU 1010 x Jaya (86.33%) in boro-2015 and MTU 1010 x Krishna Hamsa (95.67%) in kharif-2015. Jaya x Krishna Hamsa was most cold tolerant cross over both boro seasons. Significant positive correlation was observed among survival per cent, chlorophyll content, relative water content and membrane stability index over seasons
Induced chlorophyll mutations in bell pepper (Capsicum annuum L. var. grossum)
Get PDFAn investigation entitled “Induced chlorophyll mutations in bell pepper (Capsicum annuum L. var. grossum)” was conducted during kharif (summer-rainy season) 2012 and 2013 at Experimental farm of the Department of Vegetable Science and Floriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, H.P., India. Healthy seeds of California Wonder of bell pepper were exposed to physical mutagen Gamma rays using 60CO as a source of radiation at Mutation Breeding Centre, Department of Biotechnology, BARC Trombay, Mumbai and chemical mutagen EMS (Ethyl Methane Sulphonate) to obtain the spectrum and frequency of chlorophyll mutations in M2 generation. The M1 generation was produced from these mutagen treated seeds. Several unique and interesting chlorophyll and viable mutants were obtained in M2 generation. In M2 generation, gamma rays induced higher proportion of chlorophyll mutants then EMS. A progressive increase in mutation frequency of chlorophyll mutations was observed with increasing doses/concentrations. Four different types of chlorophyll mutants namely xantha, yellow xantha, chlorina and viridis were induced. Out of these mutants, chlorina and viridis were most frequent and were produced even in lower doses/concentrations while yellow xantha was least frequent and produced only in higher doses. The highest frequency of chlorophyll mutations (18.8 %) was reported in the 22 kR of gamma dose, while the lowest (0.80 %) frequency of chlorophyll mutations was found in the treatment of 1.0 % EMS. There was a dose dependent increase in the spectrum and frequency of chlorophyll mutations. These chlorophyll mutants induced by gamma radiation and EMS could be used in mutation breeding programme for inducing viable mutations for improvement of bell pepper varieties
Effect of weed management on yield and nutrient uptake in mustard (Brassica juncea)
Get PDFField investigation was carried out during rabi season of 2014-15 at Udaipur to evaluate the effect of different weed management practices on yield and nutrient uptake of mustard. The maximum seed yield was registered with two hand weeding (1955.25 kg ha-1) except weed free check and was at par with fluazifop-p-butyl 0.055 kg ha-1 10 DAS + hoeing 40 DAS and fenoxaprop-p-ethyl 0.075 kg ha-110 DAS + hoeing 40 DAS. The highest amount of total N, P and K (112.61, 25.31 and 76.90 kg ha-1, respectively) was removed by mustard in weed free treatment followed by two hand weeding while the lowest N, P and K with the values of 70.11, 16.05 and 51.86 kg ha-1, respectively was removed by weedy check followed by isoproturon 0.75 kg ha ha-1. Among the weed management practices, the total uptake of N, P and K by weeds was found significantly less in all the weed management practices as compared to weedy check(5.87, 0.86 and 5.51 kg ha-1, respectively). The least nutrient depletion by weeds was registered with the hand weeding twice (0.52, 0.08 and 0.49 kg ha-1, respectively) followed by fluazifop-p-butyl 0.055 kg ha-1 10 DAS + hoeing 40 DAS and fenoxaprop-p-ethyl 0.075 kg ha-110 DAS + hoeing 40 DAS. Use of post emergence herbicides of ‘fop’ group such as fluazifop-p-butyl, quizalofop-p-ethyl, fenoxaprop-p-ethyl (which are mostly used in soybean and groundnut crop) in indian mustard found most effective in controlling grassy weeds in early stage whereas at latterly, one hoeing 40 DAS was found effective in controlling grassy as well as broad leaved weeds under irrigated conditions
Influence of rice varieties and fertility levels on performance of rice and soil nutrient status under aerobic conditions
Get PDFScarcity of water resources in India is limiting the production of flooded rice crop. A field experiment was conducted in factorial RBD with sixteen treatment combinations including four rice varieties viz. V1: PR-115, V2: DRRH-3, V3: PAC-837 and V3: PR-121 and four fertility levels viz. F1: control (N0P0K0), F2: N, P2O5, K2O at 90: 45: 22.5 kg ha-1, F3: N, P2O5, K2O at 120:60:30 kg ha-1 and F4: N, P2O5, K2O at 150: 75: 37.5 kg ha-1 replicated thrice. The results revealed that among the different varieties, PAC-837 recorded highest plant height and number of tillers m-2 at all the growth stages. All yield attributes viz. number of effective tillers m-2, number of seeds per panicle, 1000-grain weight, spikelet sterility and grain yield, straw yield and harvest index significantly differed in different varieties and were recorded highest in PAC-837 which were however, statistically at par with DRRH-3 except grain yield and effective tillers m-2. Rice variety PAC-837 recorded highest grain yield of 45.65 q ha-1 and straw yield of 59.98 q ha-1. Among the fertility levels, number of effective tillers m-2 significantly increased up to 120: 60: 30 N-P2O5-K2O kg ha-1 thereafter nonsignificant differences were noticed. The grain and straw yield increased up to 150: 75: 37.5 N-P2O5-K2O kg ha-1 and recoded grain yield of 52.78 q ha-1 and straw yield of 73.85 q ha-1 with application of 150:75:37.5 N-P2O5-K2O kg ha-1. Nutrient application of 150: 75: 37.5 N-P2O5-K2O kg ha-1 recorded highest available N (238.16 kg ha-1), P (16.18 kg ha-1) and K (163.25 kg ha-1) in soil after harvest of the crop. Higher available nitrogen (235.28 kg ha-1), phosphorus (16.38 kg ha-1) and potassium (154.24 kg ha-1) n soil were recorded with PR-121 and lowest available major nutrients were recorded with variety PAC-837. This study would help to introduction and adaptation of new rice variety PAC-837 with application of 150: 75: 37.5 N-P2O5-K2O kg ha-1 under aerobic conditions in Shiwalik foothills region of North-Western Himalayas
Association analysis for yield and related traits in fenugreek (Trigonella foenum-graecum L.) under different environmental conditions
Get PDFSixteen diverse genotypes of fenugreek (Trigonella foenum-graecum L.) were grown in five (E1 to E5) environments which were created by different date of sowing during the rabi seasons at the Vegetable Farm of CCS HAU, Hisar. (29°15?N, 75°69?E) during 2012-13. Observations were recorded on ten randomly selected plants from each genotypes in each replications for characters viz. field emergence index, days to 50 % flowering, plant height, number of pods per plant, number of branches per plant, number of seeds per pod, pod length, seed yield (q/ha), test weight, seed germination, seed vigour index-I and II. The estimation of genotypic and phenotypic coefficients (GCV and PCV) variation in all the environments was consistently decreasing with the delaying in sowing date for all the character studied except plant height and test weight indicating that the environmental influence was comparatively more pronounced for these characters in expressing the phenotypic performance of different genotypes. Highest GCV and PCV was estimated as 50.36 % and 55.93 %, respectively for seed vigour index-I in E1. High value of heritability estimated for characters seed yield, seed vigour index-II, seed germination and branches per plant (above 70 %) in E1 revealed that these were less influenced by environment and low heritability estimated for days to 50 % flowering in E2, plant height in E2, seeds per pod in E3, field emergence index in E5 indicated high influence of environment. Based on environmental indices, the environment E2 was most favourable for all the characters studied except field emergence index