Journal of Applied and Natural Science
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Bio-efficacy of pyrazosulfuron ethyl 10% wp against weeds in transplanted rice
Get PDFAn experiment was conducted during Kharif 2012 and 2013 at Agricultural Research Station, Dhadesu-gur, University of Agricultural Sciences, Raichur, Karnataka, India, to evaluate the phytotoxicity and bio efficacy of pyrazosulfuron ethyl 10 % WP (wettable Powder) @ 5, 10, 15 and 20 g a.i./ha against the weeds in transplanted rice. Sprays of Saathi (Market Sample) @ 15 g a.i./ha (gram active ingredient/hectare), Pretilachlor 50% EC @ 500 ml a. i/ha, hand weeding at 15 and 40 days after planting (weed free check) and a weedy check (untreated check) were also maintained. The dominant weeds were Echinochloacolona, Panicum repens, Cynodondoctylon, Lud-wigiaparviflora, Leptochloachinensis and Cyperus sp. Application of pyrazosulfuron ethyl 10 % WP @ 20 g a.i./ha was recorded significantly higher grain yield (6266 kg/ha) by controlling the associated weeds in transplanted rice without any phytotoxic effect
Effect of paper industry effluent on enzyme activity and protein profiling of Chickpea (Cicer arietinum L.)
Get PDFChickpea (Cicer arietinum L.) is a legume which is mostly cultivated in India than other countries that can give significant amounts of dietary minerals and protein to humans. The effect of paper industrial effluent on chick-pea (C. arietinum L.) were analysed along with different concentration (10%, 20% 40%, 60% 80% and 100%) and pure tap water as a control to compare the effect of paper industrial effluent for one week. The amount of protein were comparable with control, their amount was increased at 40% in effluent treated seeds. The maximum activity of enzymes was found below 40% level of effluent. In this study protein profile of imbibed seeds, shoot, root and residual cotyledons were examined under the different concentration of effluent. Polyacrylamide gel electrophoresis of total protein showed that the maximum number of protein bands seen in the imbibed seeds whereas minimum number of protein bands observed in the root. SDS-PAGE revealed that less degradation and/or more rapid accumulation of proteins occurred in higher molecular weight proteins. From this study, it is clear that the industrial effluent rich in organic matter and plant nutrients are finding their use in agriculture as the cheaper way of disposal
Morphological characterization of Ampelomyces spp., a hyperparasite of Bhendi (Abelmoschus esculentus (L.) Moench) powdery mildew
Get PDFAmpelomyces is a naturally occurring hyperparasite on powdery mildews. Survey was conducted in major bhendi (Abelmoschus esculentus (L.) Moench) growing districts of Tamil Nadu during June 2014 to assess the incidence of powdery mildew and to collect different isolates of Ampelomyces spp. The results of the survey revealed that the disease incidence ranged from 15.54 to 63.45 %. Ten isolates of Ampelomyces spp. were collect-ed from surveyed areas of powdery mildew. Isolation of Ampelomyces spp. was done from powdery mildew infected bhendi leaf parasitized by Ampelomyces spp. using tissue segment method. All the isolates were identified by their morphological characters. The colour of the colonies in various medium was brownish black to greenish white. Most of the isolates showed radial and fluffy growth pattern with raised growth. The pycnidia of different isolates of Ampelomyces varied in their shape and were mostly ovoid, ellipsoid, cylindrical, pyriform to globose in shape. The size of pycnidia varied from 29.2-72.5×22.4-43.1 ?m. The number of pycnidia was found to be more in isolates viz., TNAU-AQ101 and TNAU-AQ103. Pycnidiospores are hyaline, unicellular and guttulate in shape. The pycnidial production was higher in TNAU-AQ101 and TNAU-AQ103. Application of agrochemicals is one of the oldest and most effective methods to manage powdery mildew disease. However, incessant use of these agrochemicals has many demerits such as development of resistance to pathogens, residual toxicity and environmental pollution. Hence, search for an alternative means for disease management is envisaged. The genus Ampelomyces are the major antagonists as an alternative of Erysiphales fungi being a significant group of phytopathogens
Biology of pink stem borer, Sesamia inferens (Walker) on maize, Zea mays
Get PDFBiology of pink stem borer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae) was conducted during 2015-16 in laboratories of CCS Haryana Agricultural University, Regional Research Station, Karnal on HQPM 1 (hybrid) and HKI 1128 (inbred) for two generations at room temperature. Results on biology of S. inferens in the first generation revealed that incubation period varied from 10-14 days on HQPM 1 and 11-15 days on HKI 1128. The larval duration lasted for 21-37 days on HQPM 1 and 24-39 days on HKI 1128. The adult longevity of male and female ranged from 6-7 days and 7-8 days on HQPM 1 and 5-7 days and 6-7 days on HKI 1128, respectively. The total life span ranged from 63-72 days for female and 45-58 days for male on HQPM 1 and 65-74 days for female and 49-62 days for male on HKI 1128, respectively in the first generation. The total life span in second generation ranged 94-107 days for female and 83-96 days for male on HQPM 1 and 98-112 days for female and 86-101 days for male on HKI 1128. The biology of an insect pest is a condition precedent to find out its management strategies. The biology of S. inferens on maize has not yet been studied in north western part of the country. Having regards to the fact that no systematic work on this aspect has been carried out, studies were conducted on biology of this pest for developing efficient pest management strategies
Effect of proline and salicylic acid on germination and antioxidant enzymes at different temperatures in Muskmelon (Cucumis melo L.) seeds
Get PDFEffect of different seed treatments hydration, warm water, proline (10mM and 20mM) and salicylic acid (0.1mM and 0.5mM) was investigated on percent germination and activity of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) at different temperatures in Muskmelon (Cucumis melo L.) seeds. It was observed that lower temperature (20ËšC) had decreased seed germination and activity of various anti-oxidant enzymes. Various seed treatments increased percent germination and activity of these enzymes as com-pared to control at both the temperatures. Proline 20mM (96.6) and SA 0.1mM (91.6) showed better results as com-pared to proline 10mM (95.0) and salicylic acid 0.5mM (86.6) respectively. Thus, proline and salicylic acid play an ameliorating role on low temperature stress by enhancing the activities of antioxidant enzymes and scavenging the free radicals
Non parametric measures to estimate GxE interaction of dual purpose barley genotypes for grain yield under multi-location trials
Get PDFGxE interaction of seventeen dual purpose barley genotypes evaluated at ten major barley locations of the country by non parametric methods. Non parametric measures had been well established and expressed ad-vantages over their counter parts i.e. parametric measures. Simple descriptive measures based on the ranks of gen-otypes i.e. Mean of ranks (MR) pointed towards RD2925 and BH1008 and standard deviation of ranks (SD) for KB1401 and UPB1054 whereas Coefficient of variation (CV) for JB322 and RD2925 as stable genotypes. Nonpara-metric measures based on original values (Si1, Si2, Si3, Si4, Si5, Si6, Si7) indicated the stable performance of NDB1650, JB322 and UPB1054 while UPB1053, RD2715, RD2927 and RD2035 were observed of unstable nature. CSi1, CSi2, CSi3, CSi4, CSi5, CSi6 and CSi7 measures based on the ranks of corrected grain yield identified JB322, RD2552, RD2925 and NDB1650 as stable genotypes. Spearman’s rank correlation established highly significant positive correlation of yield with SD (0.67), Si1(0.65), Si2(0.59), Si5(0.68), Si7(0.67) whereas negative association observed for CMR (Mean of corrected ranks) (-0.62), CMed (Median of corrected ranks) (-0.60). NPi(2) expressed negative correlation with CV(-0.32), Si6 (-0.30), CMR(-0.34) and CMed(-0.48). More over NPi(3) maintained negative correlation with most of the measures though the magnitude was of low magnitude
Influence of crop geometry and cultivars on growth, yield and production efficiency of dry direct-seeded rice (Oryza sativa L.)
Get PDFA field experiment was conducted during kharif (summer) season of 2014, aim of the experiment was to investigate suitable crop geometry and cultivar and their influences on performance of dry direct-seeded rice (Oryza sativa L.), experimental treatments were consisting of 15 treatments, namely, main plots: five cultivars (MTU 7029, NDR 97, HUR 105, HUR 4-3 and PRH-10) and sub-plots: three crop geometry’s (20 x 10, 20 x 20 and 25 x 25 cm2). All the data recorded were statistically analyzed using the standard procedures of split-plot design. The results indicated that amongst cultivars, aromatic rice hybrid PRH-10 recorded significantly more grain yield (5582.32 kg/ha) than cultivar HUR 4-3 (4612.99 kg/ha) and NDR 97 (3397.82 kg/ha), whereas; it was statistically comparable with cultivar MTU 7029 (5489.24 kg/ha) and HUR 105 (5022.03 kg/ha). The cultivar PRH-10 also registered higher gross return (105771.9 `/ha), net return (66389.08 `/ha) and production efficiency (592.76 `/ha/day) than the remaining cultivars. The higher grain yield of PRH-10 over these cultivars was due to considerable improvement in most of its yield attributing characters like panicle length (27.92 cm), a number of grains/panicle (178.70) and test weight (26.35 g). In a case of crop geometry treatment, plant spacing of 25 x 25 cm2 recorded higher grain yield as compared to remaining plant spacing while the plant spacing of 20 x 10 cm2 recorded higher gross return, net return and production efficiency as compared to 20 x 20 and 25 x 25 cm2. Plant geometry plays an imperative role towards improving the grain yield of cultivars in direct seeded rice by optimal utilization of natural resources. Therefore, for getting higher net return and production efficiency, cultivar PRH-10 at plant spacing 20 x 10 cm2 can be raised in dry direct-seeded rice in Varanasi region of Eastern Uttar Pradesh
Evaluation and diversity analysis in Indian mustard [Brassica juncea (L.) Czern & Coss.] germplasm accessions on the basis of principal component analysis
Get PDFPrincipal component analysis was carried out with 20 morphological traits (including quantitative as well as qualitative) among 96 germplasm lines of Indian mustard [Brassica juncea (L.) Czern & Coss.]. Principal factor analysis led to the identification of eight principal components (PCs) which explained about 70.41% variability. The first principal component (PC1) explained 16.21% of the total variation. The remaining PC’s explained progressively lesser and lesser of the total variation. Varimax Rotation enabled loading of similar type of variables on a common principal factor (PF) permitting to designate them as yield factor, maturity factor and oil factor etc. Based on PF scores and cluster mean values the germplasm accessions viz., RC2, RC32 and RC51 (cluster I), RC95 and RC96 (cluster X) were found superior for seed yield/plant and yield related factors like primary and secondary branches/plant; while the accessions RC34, RC185 and RC195 (cluster III) and RC53 (cluster VIII) were found superior for oil content. These accessions may further be utilized in breeding programmes for evolving mustard varieties having high seed yield and oil content. Hierarchical cluster analysis resulted into ten clusters containing two to 26 accessions. The results of cluster and principal factor analyses were in confirmation of each other
Studies on physico-mechanical properties of W. Murcott mandarin
Get PDFThe post-harvest physico-mechanical properties of fruits is important in adoption and design of varioushandling, packaging, storage and transportation systems. These are important for sizing and grading the fruit beforemarketing as well as demarcate their end use. Geometric, gravimetric and textural properties were determined forthe fresh samples of W. Murcottmandarin. The results show that W. Murcottfruit diameter varies from 81.82-68.97mm, equatorial length varies from 86.51-68.43 mm, and length varies from 61.33-52.08 mm. The specific mean areais 57.591 with the sphericity of 0.90. The L, a, b values were 53.37, 42.9 and 49.68 respectively. The firmness variedfrom 1.351-1.650 kgf. These physical attributes can be taken into consideration while designing the grading andprocessing equipment
Tannery effluent effect on the haematological parameters of freshwater fish, Channa punctatus
Get PDFThe present investigation was undertaken to evaluate the tannery effluent toxicity stress symptoms in fish blood during a long term of exposure period. The effect of tannery effluent on various haematological parameters were evaluated exposing fresh water fish, C. punctatus to different concentration i.e., [Control, 5% Tannery effluent (TE), 10% TE and 20% TE] of tannery effluent. Exposed of fish to tannery effluent showed a significant decrease in the haemoglobin (Hb) content (9.16± 0.08), red blood cells (3.32 ± 0.12), packed cell volume (34.66 ± 0.33) and mean corpuscular haemoglobin (MCH) values, whereas significant increase in the white blood cells (WBC), erythro-cyte sedimentation rate (ESR) and clotting time was recorded with increase in exposure periods as compared to control respectively. Hb, RBC and MCHC values showed fluctuating results. The haematological parameters were decreases from 15th days of exposure periods to 45th days of exposure period. The decrease in haematological parameters clearly indicates that the exposed fishes have become anemic due to tannery effluent exposure