Journal of Applied and Natural Science
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Genetic study for epistatic gene effects for major yield contributing traits against drought in rice
Get PDFThe study subjected to estimate gene effects and inheritance of quantitative traits of rice with Generation Mean Analysis (GMA). Segregation analysis and estimation of genetic parameters under epistatic model indicated partial dominance and importance of additive effects in the inheritance of drought tolerance, respectively. In present study, absence of epistasis by scaling tests was recorded only for plant height in cross NDR-359 x P0 1564, grains per panicle in cross DSL- 63-8 x NDR- 359, test weight in cross Sarjoo-52 x P0 359, harvest-index in cross NDR-359 x P0 1564 and spikelets per panicle in cross Sarjoo-52 x P0 359 and NDR-359 x P0 1564 in irrigated condition and days to 50 per cent flowering in cross P0 359 x Sonam and harvest-index in cross NDR-359 x P0 1564 in drought condition. In remaining cases, existence of epistasis was observed in either one or both conditions by one or both types of scaling tests. The presence of complementary epistasis in cross P0 359 x Sonam and P0 1564 x Sarjoo-52 would make progress through selection procedures exploiting additive gene actions faster while existence of duplicate epistasis in cross NDR-359 x P0 1564 would have reverse impact. The non-additive gene effects in NDR-359 x P0 1564 for grain yield plant-1may also be utilized for facilitating development of pureline cultivars by involving population improvement methods. Our study concluded that for a large number of traits in six crosses, dominance gene effects and epistatic interactions were significant under drought and irrigated conditions implying that utilization of heterosis through hybrid varieties will act as a budding choice
Comparative study of soil properties and vegetation at various open dump and non-dumpsites in the Bengaluru city of Karnataka, India
Get PDFA comparative field studies on seven municipal dumpsites namely Agara 1 (12.917°N , 77.639°E), Agara 2 (12.922°N, 77.639°E), HSR depot (12.919°N, 77.644°E) , Koramangala Church (12.934°N, 77.626°E) , Koramanagla BDA (12.931°N, 77.625°E), Garvebhayipalya (12.897°N, 77.638°E) and Sanjay Gandhi hospital (12.891°N, 77.601°E), and its adjoining non-dump sites were conducted to understand their soil characteristic features and the vegetation pattern. Soil characteristics were presented in terms of the physicochemical parameters and the vegetation patterns were presented in terms of the dominance using the ecological parameter Important Value Index (IVI). Soils at the dump sites showed higher mean electrical conductivity and pH values as compared to the non-dump sites. Though the mineral content showed higher mean value in the dump sites (except chloride), there is no significant variation in the higher total soluble solutes between dump and non-dump sites(P>0.05) As per ANNOVA there was highly significant variation in the heavy metal content between dump and non dumpsites (P<0.01).. With respect to vegetation analysis though 50 different species found across locations only 10 species viz Alternatheria sessile, Amaranthus spinose, Caesalpinia pulcherima, Ipomea acumilanata, Ipomea evolvulus, Parthenium hysterophorous Pisum sativum, Ricinis communis, Sida rombifolia and Solanum lycopersicum were found consistent across all locations irrespective of the seasons. Among these, A. sessile, R. communis and A. spinosa were found dominant based on the IVI values across seven locations which further can be studied for their potential for phyto remediating the land pollutants such as heavy metals. 
Stomatal Index of a few common tree species in the Kishangarh Marble Slurry Dump-yard area, Ajmer (Rajasthan): A case study
Get PDFThe size and number of the stomata present on the foliar epidermis of Tree species is directly influenced by the Suspended Particulate Matter. Marble dust is a very fine powder having approximately 40% particles below 75?m diameter of which approximately 30% are having a size less than 25?m present in the atmosphere. A comparative micro-morphological study of stomatal index of the dominant tree speciesAlbizialebbeck, Azadirachta indica, Callistemon lanceolatus growing in the vicinity of Marble slurry dump-yard, Kishangarh (Ajmer) was done from the month of April 2014 to March 2015.The stomatal index of the lower epidermis of A. indica and A. lebbeck leaves from polluted sites was found to have greater value as compared to that with those of unpolluted site during the months of May to October while in case of the rough, leathery leaves of Callistemon lanceolatus, the stomatal index value in leaves from polluted site was less during these months. This may be attributed to the prevailing winds and weather conditions in the area of study which play a crucial role in the accumulated particulate matter on the phylloplane of the selected tree species viz.A. lebbeck, A. indica,C. lanceolatus
Effect of feeding swill feed supplemeted with chocolate cream waste on backfat thickness and body measurements in fattener weaner pigs
Get PDFPig rearing on concentrate feed may not be economical because of higher market price of raw materials. The alternative source could be the use of swill feeding. Consequently, the impact of the swill feed on the backfat thickness and body measurements calls for investigation against any other alternative feed. Hence in the present study, an attempt has been made to compare the body measurements and backfat thickness in fattener piglets which were fed with swill feed (T1) vs. Those fed with chocolate cream waste supplemented with swill feed (T2). The statistical analysis of the data obtained in the present study revealed that there was highly significant (P?0.01) difference between body weight gain and backfat thickness (layer I and II) of both the group of piglets which were taken for trial starting from three months of age till five months of age. However, not significant difference (P?0.05) was noticed for body length and chest girth except in the pigs of 150 days age group. This study indicates that swill feed supplemented with chocolate cream waste could be used to reduce the feed cost since higher weight gain is achieved in a shorter time interval, increasing body measurements and backfat thickness compared to swill feed (T1) group of piglets
Study of different isozymes in Pinus gerardiana Wall.
Get PDFPinus gerardiana (Chilgoza) is a small to medium sized evergreen tree yielding a highly valuable edible nut. The present investigations were undertaken to assess the different isozymes in Chilgoza pine. Seven isozymes viz., MDH (Malate dehydrogenase), GDH (Glutamate dehydrogenase), SKDH (Shikimic acid dehydrogenase), 6PGDH (6-Phosphogluconate dehydrogenase), MNR (Menadione reductase), IDH (Isocitrate dehydrogenase) and ADH (Alcohol dehdrogenase) were studied. A total of sixteen gene loci were recorded for seven enzyme systems out of which 6 loci namely MDH- A, 6PGDH- A, SKDH- A, IDH- B, MNR- B and ADH- A were polymorphic whereas the remaining ten gene loci i.e. MDH- B, MDH- C, MDH- D, GDH- A, 6PGDH- B, SKDH- B, IDH- A, MNR- A, MNR- B and ADH- B showed no variation. The percentage of polymorphic loci and average number of alleles per locus are one of the useful criterions for comparing species of populations for genetic diversity
Profitability of Integrated Farming System model for small and medium farmers of South Tripura, Tripura
Get PDFThe present study was carried out under Krishi Vigyan Kendra, South Tripura to study the production, profitability and employment generation of IFS over prevailing conventional rice-rice system of farming in South Tripura district of Tripura during 2015-16, 2016-17 and 2017-18. The components rice, maize, vegetables, ginger, apiculture, fishery, poultry and piggery were considered for the study under integrated farming system. The Integrated Farming System (IFS) model showed 17.79 % increase in productivity and 48.91 % higher net return over conventional rice-rice system. Among the components evaluated, the highest per cent net return was received from Fishery unit (31.78), followed by Piggery unit (18.19), Apiculture (13.93), Poultry unit (12.96), Spice Ginger (10.19), Crop (7.31) and Vegetables (5.64) respectively. The highest B:C ratio (3.67) was obtained from fishery unit among all the component evaluated under the IFS. A total of 289 Man days/ha/year employments can be generated under Integrated Farming System. This system of IFS model may be useful in the areas where water is limiting and an efficient alternate system of conventional rice-rice system
Polygalacturonase production by Aspergillus nomius MR103 in solid state fermentation using Agro-industrial wastes
Get PDFThe present study was aimed at polygalacturonase production from Aspergillus nomius MR103 under solid state fermentation. A total of 57 fungal strains were obtained from mangrove soils collected from Gilakaladindi and Malakayalanka of Krishna District Andhra Pradesh. For the isolation of fungi these Soil samples were serially diluted and plated on pectin agar media plates. Among them, the isolate which showed maximum polygalacturonase activity was selected for this study. This strain was identified as A. nomius MR 103 by 18S rRNA sequences analysis. Pectin rich agro-industrial wastes like apple peel, citrus peel, orange peel, wheat bran, rice bran and sugarcane bagasse were used as substrates for polygalacturonase production by A. nomius MR 103. This strain was inoculated into the nutrient broth containing agro industrial wastes under solid state fermentation and amount of Polygalacturonase production was estimated. Maximum enzyme production of 4.83 IU/mg was recorded at pH 7.0 and temperature 35?C after 7 days of incubation, when orange peels were used as substrate. Addition of carbon and nitrogen sources to orange peel media improved the Polygalcturonase production. Sucrose as carbon and peptone as nitrogen sources were proved to be the best for maximum production of Polygalcturonase by A. nomius MR 103 on orange peel substrate. Utilization of agro-industrial by-products provided the establishment of a cost-efficient and sustainable process for enzyme production. 
Development of spiced squash (appetizer) from wild prickly pear (Opuntia dillenii Haw.) and its quality evaluation during storage
Get PDFWild prickly pear (Opuntia dillenii Haw.) is one of the wild fruit with great importance because of its high antioxidants, colour pigments and other quality parameters besides its medicinal properties. In India, there are only a limited number of reports pertaining to utilization of this fruit which further lack the development of value added novel products. So, the present studies were carried out for the development of spiced squash or appetizer from wild prickly pear fruit and its quality evaluation during storage. Different combinations of fruit juice (25, 30, 35 and 40 %) and sugar syrup (40 and 45 °B) were tried to standardize a proper combination for appetizer. The appetizer prepared by using the best recipe with 35 % juice, 45 °B TSS (Total soluble solids) and 1.20 % acidity was packed in two packaging materials viz. glass and PET (Polyethylene terephthalate) bottles which were further stored for six months under ambient and refrigerated temperature conditions. Appetizer packed in glass as well as in PET packaging material can be stored successfully for a period of six months under both the temperature conditions. However, various quality parameters of appetizer were retained higher in glass bottles stored under refrigerated storage conditions
Potential of distillery effluents for safe water through vermifiltration
Get PDFVermifiltration of wastewater using waste eater earthworms is a newly conceived novel technology. The present study evaluated BOD, COD and TS showing significant variation in decrease by 95%, 90% and 80% respectively through vermifiltration of distillery effluents. The nutrient contents (TN, TP, TK, TCa and TMg) in the vermicasts had increase (1.82 % in TN, 0.24% in TP, 2.15% in TK, 2.07% in TCa and 2.86 % in TMg) in the range of fold than the control level. The morphology of the control and experimental vermicast samples were analyzed with SEM and the image showed significant variation. The FT-IR spectrum analysis showed reduction of aliphatic/aromatic (C=C and OH) compounds in the vermicompost. Thus, the present study significantly highlights the vermifiltration technology in treating distillery effluent
An annual algal diversity of Lakhna, Etawah, Uttar Pradesh, India
Get PDFThe nature of an ecosystem can be easily assumed by the presence of planktonic diversity, as they have a major role in oxygen amelioration, binding and removal of toxic substances from water body. The present enumeration deals with the annual algal diversity from the Lakhna town of Etawah, Uttar Pradesh. During this one year period, total fifty-four species of Algae recorded viz. Achnanthes minutissima, Amphora ovalis, Anabaena oscillarioides, A. oryzae, Ankistrodesmus falcatus, Aphanocapsa littoralis, Aphanothece microscopica, Arthrospira sp., Calothrix gloeocola, Chlorella vulgaris, Chlorococcum humicola, Chroococcus minor, C. minutes, Cladophora glomerata, Closterium venus, Coelosphaerium kuetzingianum, Cyclotella meneghiniana, Cylindrospermum minutissimum, Euglena minuta, Fragilaria crotonensis, Gloeocapsa magma, Gloeotrichia pisum, Gomphonema parvulum, Hydrodictyon reticulatum, Lyngbya contorta, L. epiphytica, L. majuscula, Merismopedia glauca, M. tenuissima, Microcystis aeruginosa, M. flos-aquae, M. robusta, Mougeotia calcarea, Navicula ambigua, N. brebissonii, N. lata, Nostoc commune, N. punctiforme, Oscillatoria formosa, O. subuliformis, O. princeps, Pediastrum boryanum, Phormidium ambiguum, P. fragile, P. lucidum, Rivularia aquatica, Scenedesmus bijuga, S. obliquus, Spirogyra affinis, S. submaxima, Spirulina gigantea, S. major, Ulothrix zonata, Zygnema collinsianum. This information can be used as baseline data and may be further used to assess any change in algal diversity of Gangetic plain after a sufficient gap to understand the impact of changing climate on it