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Dynamics of the gut microbiome of Asian seabass (Lates calcarifer) following oral vaccination and challenge with virulent Vibrio harveyi
No full textThis study investigates the dynamics of the gut microbiome of Asian seabass following oral vaccination and challenge with virulent Vibrio harveyi. Fish were divided into vaccinated and non-vaccinated groups and fed either with commercial pellets containing an inactivated V. harveyi Vh1 vaccine or commercial pellets only. The feed-based vaccine was administered at weeks 0 (prime vaccination), 2 (first booster) and 6 (second booster) for the field testing, and the fish were transferred and challenged with 108 CFU/mL of virulent V. harveyi Vh1 in the laboratory at 70 days after prime vaccination. Gastrointestinal samples were aseptically collected at pre-vaccination, post-vaccination, and post-challenge for 16S amplicon sequencing. The gut microbial diversity and richness of the vaccinated group were drastically increased following the vaccination regimen but sustained following the bacterial challenge. However, for the non-vaccinated group, the diversity and richness slightly increased as the fish grow throughout the culture period but drastically increased following the bacterial challenge. The post-challenge phase revealed that Firmicutes, Fusobacteria, Bacteroidetes, Photobacterium, and Cetobacterium were higher in the vaccinated fish, whereas Proteobacteria, Actinobacteria, Shewanella, Vibrio, and Clostridium were higher in non-vaccinated group. Potential KEGG ortholog biomarkers that involve in quorum sensing pathway were identified only in the non-vaccinated group at post-challenge. Post-challenge showed a significant difference of relative percentage survival between the vaccinated (80 %) and non-vaccinated (0 %) groups. This study showed that feed-based vaccination helps to sustain the gut microbiota stability and reduce the mortality following Vibrio infection
Kampus satelit UPM, buka peluang dalam mobiliti pelajar dan penyediaan pendidikan global
Get PDFSERDANG, 4 Februari - Universiti Putra Malaysia (UPM) telah menjalinkan kerjasama dengan Shandong Women’s University (SDWU), China, melalui Memorandum Persefahaman (MoU), bagi penubuhan kampus satelit untuk membuka peluang baharu dalam akademik dan penyelidikan baru-baru ini
Future of reproductive biotechnologies in water buffalo in Southeast Asian countries
No full textThe future of reproductive biotechnologies in water buffalo in Southeast Asian countries holds significant promise for enhancing genetic quality and productivity. Fixed-time artificial insemination remains the commonly used technology, with advances in assisted reproductive technologies (ART) such as in vitro embryo production (IVEP), embryo transfer (ET), and the use of sex-sorted sperm increasingly adopted to improve breeding efficiency. These technologies overcome traditional breeding limitations, such as low reproductive rates, genetic diversity constraints, and the production of sex-predetermined offspring. The application of multiple ovulation and embryo transfer (MOET) is constrained by poor embryo recovery in this livestock species. Somatic cell nuclear transfer (SCNT) offers great potential for producing sex-predetermined and genetically superior buffalo but requires further research to increase efficiency. Cryopreservation of buffalo genetics is bolstered by the establishment of Gene Banks. Challenges such as high costs, the need for skilled personnel, and infrastructure development remain constraints. Integration of genomic selection, automation, and expansion of ET programs are clear directions. Strengthening research and collaboration among Southeast Asian countries is essential to fully realize the benefits of these biotechnologies and ensure sustainable and profitable buffalo farming