InterNano Nanomanufacturing Repository
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Development of fluorescent nanoparticle-labeled lateral flow assay for the detection of nucleic acids
No full textThe rapid, specific and sensitive detection of nucleic acids is of utmost importance for the identification of infectious agents, diagnosis and treatment of genetic diseases, and the detection of pathogens related to human health and safety. Here we report the development of a simple and sensitive nucleic acid sequence-based and Ru(bpy)(3)(2+)-doped silica nanoparticle-labeled lateral flow assay which achieves low limit of detection by using fluorescencent nanoparticles. The detection of the synthetic nucleic acid sequences representative of Trypanosoma mRNA, the causative agent for African sleeping sickness, was utilized to demonstrate this assay. The 30 nm spherical Ru(bpy)(3)(2+)-doped silica nanoparticles were prepared in aqueous medium by a novel method recently reported. The nanoparticles were modified by 3-glycidoxypropyl trimeth oxysilane in order to conjugate to amine-capped oligonucleotide reporter probes. The fluorescent intensities of the fluorescent assays were quantified on a mictrotiter plate reader using a custom holder. The experimental results showed that the lateral flow fluorescent assay developed was more sensitive compared with the traditional colloidal gold test strips. The limit of detection for the fluorescent lateral flow assay developed is approximately 0.066 fmols as compared to approximately 15 fmols for the colloidal gold. The limit of detection can further be reduced about one order of magnitude when ``dipstick'' format was used
One-way wicking in open micro-channels controlled by channel topography
No full textOne-way wicking (microfluidic diode) behaviors of a range of IPA-water mixtures on internally structured PDMS-based open micro-channels were experimentally demonstrated and quantified. The open microfluidic channels, each internally decorated with an array of angled fin-like-structure pairs, were fabricated using a combined photolithography and soft molding procedure. Propagations of wetting fluids were found to be much more impeded on the fin-tilting direction, or the hard wicking direction, comparing to the opposite direction, or the easy wicking direction. This asymmetric wicking behaviors were attributed to the structure-induced direction-dependent Laplace pressure. Two key parameters - the contact angle of the wicking fluid and the tilting angle of the fin-like structures - were studied. The effects of preferential evaporation and wetting instability were also investigated. The findings of this study are expected to provide a better understanding of how fluids interact with micro-scaled structures and to offer a new way of manipulating fluids at the micron and nanometer stales. (C) 2013 Elsevier Inc. All rights reserved
Cross-Linked Block Copolymer/Ionic Liquid Self-Assembled Blends for Polymer Gel Electrolytes with High Ionic Conductivity and Mechanical Strength
No full textA novel method for the preparation of Ru(bpy)(2+)(3) -doped silica nanoparticles
No full textA novel method for the preparation of spherical Ru(bpy)(2+)(3) -doped silica nanoparticles with adjustable diameters and narrow particle size distribution with high yield was developed. Unlike the most commonly used Stober method which uses a reverse microemulsion system (oil-based, NH4OH as a catalyst, pH 11-12), the proposed method synthesizes the nanoparticles in a water-based system (pH 9-10) using L-lysine as a catalyst in the presence of the dye. Both the size of the spherical dye-doped nanoparticles and the Ru(bpy)(2+)(3) content which is incorporated in silica nanoparticles are precisely controlled by the multiple-step addition of tetraethyl orthosilicate and Ru(bpy)(2+)(3) solution, respectively. (C) 2012 Elsevier B.V. All rights reserved
Surface analysis and electrochemical characterization of palladium-cobalt nanoring formation from molecular precursor, Et3NH (2) CoPd2(mu-4-I-3,5-Me(2)pz)(4)Cl-4 , on highly ordered pyrolytic graphite
No full textA Pd2Co precursor, Et3NH(2)CoPd2(-4-I-3,5-Me(2)pz)(4)Cl-4, was used to synthesize palladium-cobalt nanorings and nanoparticles on highly ordered pyrolytic graphite (HOPG) surface. Different types of nanostructures were formed on HOPG surfaces and were controlled by relative humidity (\%RH). These structures included Pd2Co nanorings on HOPG surface by self-assembly with humidity control. The \%RH affects the size and dispersion of the self-formation of the Pd2Co rings on HOPG surfaces. The modified HOPG surface with Pd2Co precursor at 80\%RH has rings of similar sizes, while modification at 76\%RH gives well-formed rings and 70\%RH with smaller diameters. After thermal reduction of the Pd2Co precursor on HOPG, bimetallic nanostructures were formed. X-ray photoelectron spectroscopy, atomic force microscopy and scanning electron microscopy with energy-dispersive X-ray fluorescence spectroscopy techniques were employed to study the composition and morphology of the nanostructures formations on the HOPG surface. Electrochemical characterization of the Pd2Co nanostructures was performed. Moreover, the bimetallic catalyst has electrocatalytic activity for the oxygen reduction reaction. Copyright (c) 2013 John Wiley \& Sons, Ltd
Next Generation Nanotechnology Infrastructure Looking to Broaden Base
No full textAs the National Nanotechnology Infrastructure Network (NNIN) approaches the end of its initial 10 year term, it has clearly supported and catalyzed the growth of nanotechnology-enabled products. The NNIN user facilities have provided a key national resource where academic and industry researchers are able to access a wide range of state of the art fabrication processes and equipment to demonstrate proof of concept or prototype device technologies. In many instances, the NNIN serves to incubate innovation by enabling early stage R&D activities that otherwise may not be possible due to resource limitations, particularly for small companies. As the model for a Next Generation Nanotechnology Infrastructure (NG-NI) is being developed to accommodate emerging nanomanufacturing methods, materials, and progress towards nanoscale systems, the National Science Foundation (NSF) and other stakeholders are contemplating what capabilities should included in order to continue and accelerate the development of nanotechnology-enabled products, and further expand the innovation pipeline.
Also included: InterNano Editorial Commentary: Nanomaterials in Food and Food Packaging, Cabot Launches First Graphene-Based Additive to Improve Energy Density of Lithium-Ion Batteries, and NanoBusiness Interview – Douglas Jamison Chairman & CEO Harris & Harris Group, Inc
Nanomanufacturing Summit 2013
No full textThe nanotechnology innovation ecosystem within the U.S. remains firmly anchored by the fundamental science activities supported by federal and state government investments, being well positioned to accelerate the commercialization path for nano-enabled products. Effective technology transfer of translational R&D from laboratory proof-of-concept and prototype demonstrations remains a significant barrier to rapid commercial scale-up, and numerous models have emerged to better support this critical stage of the innovation cycle, including regional and state models for stakeholder networks providing the critical connections between academic research, investors, and entrepreneurs. While measures of success for an innovation ecosystem are not always so easily derived, the approach of connecting capital, resources, and leadership with good science remains an enduring model which continues to shape the future for the commercialization of nanotechnology, with nanomanufacturing firmly embedded within the framework of advanced manufacturing in the U.S. Beneq's industrial thin film coating, nanotechnology and sustainability, and metallic nanoparticles are also discussed
