Ardabil University of Medical Sciences
ArUMS Digital Repository (سامانه اطلاعات زیست پزشکی و سلامت)Not a member yet
7752 research outputs found
Sort by
LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women
No full textIntroduction: Preeclampsia is a specific pregnancy syndrome that affects all parts of the body. One of the possible causes of preeclampsia is genetic factors, including genetic predisposing genes. The aim of this study was to compare the expression level of LncRNA (large non coding RNA) TERRA (Telomeric repeat-containing RNA) and telomere length in a pair of women with preeclampsia and healthy.
Methods: In this case-control study which was performed in 2017-2018, placenta specimens were taken from 25 mothers with Pre-eclampsia who referred to Ardabil Alavi Hospital and were candidate for pregnancy termination; in the control group also, placenta specimens were taken from 25 women without preeclampsia. After extraction of RNA and cDNA synthesis, TERRA expression level was measured by Real Time PCR and the changes of Telomere length was measured by Telomere Length Assay technique. Data were analyzed by SPSS software (version 16) and Independent t-test. PResults: The mean for expression level of LNCRNA TERRA in the case group was 7.87 ± 1.9 and in the control group was 9.8 ± 2.8. Independent T-test results showed significant difference between the two groups in terms of expression level of LNCRNA TERRA (p=0.006). The mean for Telomere length in the case group was 4.35 ± 1.9 and in the control group was 6.2 ± 1.54. Independent T-test results showed significant difference between the two groups in terms of Telomere length, too (p=0.001). There was no significant correlation between maternal age and LNCRNA TERRA expression level and telomere length (p = 0.88). There was no statistically significant difference in expression level of LNCRNA TERRA and Telomere length between the sexes of newborns (p=0.1).
Conclusion: Our data identified the significant role of LNCRNA TERRA and Telomere length in Pre-eclampsia. LNCRNA TERRA and Telomere length can be used to predict and diagnose preeclampsi
The effects of Nigella sativa on respiratory, allergic and immunologic disorders, evidence from experimental and clinical studies, a comprehensive and updated review
No full textNigella sativa (N. sativa) seed had been used traditionally due to several pharmacological effects. The updated experimental and clinical effects of N. sativa and its constituents on respiratory, allergic and immunologic disorders are provided in this comprehensive review article. Various databases including PubMed, Science Direct and Scopus were used. The preventive effects of N. sativa on pulmonary diseases were mainly due to its constituents such as thymoquinone, thymol, carvacrol and alpha-hederin. Extracts and constituents of N. sativa showed the relaxant effect, with possible mechanisms indicating its bronchodilatory effect in obstructive pulmonary diseases. In experimental animal models of different respiratory diseases, the preventive effect of various extracts and constituents of N. sativa was demonstrated by mechanisms such as antioxidant, immunomodulatory and antiinflammatory effects. Bronchodilatory and preventive effects of the plant and its components on asthma, COPD and lung disorders due to exposure to noxious agents as well as on allergic and immunologic disorders were also shown in the clinical studies. Various extracts and constituents of N. sativa showed pharmacological and therapeutic effects on respiratory, allergic and immunologic disorders indicating possible remedy effect of that the plant and its effective substances in treating respiratory, allergic and immunologic
diseases
Comparison of Psychological Outcomes based on Coping Skills and Personality Traits in Parents of Children Admitted to PICU with Parents of Children Admitted to Other Wards of BooAli Hospital in Ardabil in 1398
No full textBackground: The consequences of illness and hospitalization of the child are one of the main sources of psychological stress for the family. Parents use a variety of coping strategies to mitigate these consequences. Choosing the type of parental coping strategy has a very important impact on how they cope with stress. Parents' personality traits are very important in this choice. In order to maintain peace of mind in parents and children and provide better health services, it is necessary to identify these consequences.
Aim: Determining and comparing psychological outcomes based on coping skills and personality traits in parents of children admitted to PICU and parents of children hospitalized in other wards of Bu-Ali Hospital in Ardabil in 1398.
Methods and material: This research is a cross-sectional-analytical study. The research population includes all parents of children hospitalized in Bu-Ali hospital in Ardabil. 105 samples were selected from the parents of children admitted to PICU and 105 samples were selected from the parents of children admitted to other wards. Samples of the two groups were matched. In this study, demographic questionnaire, Lazarus-Folkman coping skills questionnaire, DASS stress, anxiety and depression questionnaire and short form of NEO personality traits questionnaire were used.
Results: Comparing the parents of children admitted to PICU with other wards, the level of stress (P <0.001) and depression (P = 0.007), emotional coping strategy (P <0.001) and neuropsychological personality traits (P = 0.004) in parents of children admitted to PICU was significantly higher. It was also found that there is a significant direct relationship between emotion-oriented coping skills in the PICU group with neuropsychological and extroversion-introversion personality traits, and a significant inverse relationship with the personality component of the desire to gain new experiences.
Conclusion: Parents of children admitted to PICU are significantly more prone to psychological distress. Therefore, their need to services and psychological support seems to be very necessary
: Letter to the editor” Rectal spacing, prostate coverage, and periprocedural outcomes after hydrogel spacer injection during low-dose-rate brachytherapy implantation”
No full textWith interest, we read the recent paper by Kahn et al.,
(1) reporting dosimetric impact and periprocedural outcomes
after transperineal injection of hydrogel spacer (DuraSeal
Dural Sealant System; Covidien, Mansfield, MA)
during iodine-125 low-dose-rate (LDR) prostate brachytherapy.
They observed that using hydrogel spacer creates
a significant distance between the rectum and prostate
13.9 � 5.2 mm vs. 6.5 � 5.0 mm ( p!0.0001), resulting
in statistically significant reduction in the dose to 1 cc,
2 cc, and 5 cc of the rectum relative to prescription dose
(1). These data are in line with previous reports, investigating
dosimetric impact of hydrogel spacers during prostate
brachytherapy (2). In addition, after median followup
of 2 years, there was no significant difference in rectal
toxicity between hydrogel and nonhydrogel group (1). To
the best of our knowledge, this is the first study reporting
late rectal toxicity after hydrogel spacer injection during
iodine-125 LDR prostate brachytherapy; however, there
are some methodological issues that need to be taken into
accoun
Nrf2-ME-1 axis is associated with 5-FU resistance in gastric cancer cell line
No full textThe aim of this study was to investigate the role of nuclear factor erythroid 2-related factor 2 (Nrf2) and key downstream targeting molecule, malic enzyme-1 (ME-1), in the induction of 5-fluorouracil (5-FU) resistance. Resistant MKN-45 (MKN-45/DR) cell line was generated by employing IC50 concentration of the agent followed by a resting cycles. Cell viability and apoptosis were evaluated by MTT assay, DAPI staining and flow cytometry. mRNA expression levels of Nrf2 and ME-1 were measured by qRT-PCR technique. Morphology analysis and combination index calculation were assessed by ImagJ and compuSyn softwares, respectively. The value for IC50 in 5-FU resistant cells increased from 8.81 ± 0.209–142.4 ± 0.060 μM with a considerable morphological changes from round to elongated shape (p = 0.016). Nrf2 and ME-1 expression levels were decreased in resistant cells with a marked increase in MDR1 mRNA level compared to sensitive cells. Combination of Nrf2 inhibitors, luteolin and brusatol had synergistic effects on 5-FU induced cytotoxicity. Moreover, combined incubation of MKN-45/DR cells with these inhibitors, exponentially increased Nrf2 and ME-1 mRNA levels (p < 0.001). Our findings suggest that the combined application of Nrf2 inhibitors, can be considered as a novel strategy to overcome 5-FU resistance in GC patients
Cutaneous Leishmaniasis Situation and Predicting the Distribution of Phlebotomus papatasi and P. sergenti as Vectors of Leishmaniasis in Ardabil Province, Iran
Get PDFCutaneous leishmaniosis (CL) is the most common form of leishmaniasis.CL caused by L. major and L. tropica is endemic in 17 provinces of Iran. This study was carried out to elucidate situation of CL in Ardabil province and to predict distribution of Phlebotomus papatasi and Phlebotomus sergenti (Diptera: Psychodidae) as vectors of CL in the region. In this cross-sectional study, data on CL patients were collected from local health centers of Ardabil province, Iran during 2006-2018 to establish a geodatabase using ArcGIS10.3. A total of 20 CL cases were selected randomly and skin samples were collected and analyzed by PCR method. MaxEnt 3.3.3 model was used to determine ecologically suitable niches for the main vectors. A total, 309 CL human cases were reported and the highest incidence rate of disease was occurred in Bilasavar (37/100,000) and Germi (35/100,000). A total of 2,794 sand flies were collected during May to October 2018. The environmentally suitable habitats for P. papatasi and P. sergenti were predicted to be present in northern and central areas of Ardabil province. The most variable that contributed ratio in the modeling were Isothermality and slope factors. Ardabil province is possibly an endemic are for CL. The presence of P. papatasi and P. sergenti justifies local transmission while the vectors of CL are existing in the northern and central areas of the province
Evaluation of correlation between serum levels of Visfatin with echocardiography and angiography finding in acute myocardial infarction patients and compared their results with stable angina patients results
No full textBakground and objective: Cardiovascular diseases are among leading causes of mortality worldwide. Some knowen related risk facors are dyslipidemia, smoking and familial history.
Methods: In this case-control study, 3 groups, each including 30 people were enrolled. First group was containing AMI patients, second group was containing SAP patients and third group was containing healthy individuals that all of them had chest pain as chief comlient.
Patients divided to 3 groups according to angiographic study. Demographic, Echocardiographic, laboratory findings (such as Visfatin and IL-6) were gathered and been analysed.
Results: The mean age of patients in control group was 62.26 and in SAP group was 62.93 and in AMI group was 52.83 . Systolic blood pressure of SAP group patients was higher than other groups (p<0.001) and Diastolic blood pressure of AMI group patients was lower than other groups(p<0.002). The results suggested that Gensini Scores was significantly higher in AMI patients. Comparison of Visfatin serum levels between groups showed that serum levels of this molecule was significantly higher than other groups(p<0.001). Serum levels of IL-6 as an inflammatory factor were also higher in AMI groups patients. Also EFT and EFT index was higher in AMI patients compared to the SAP and control group. More evaluations revealed a significant correlation between Visfatin with CK-MB, HsTnT, Gensini score, EFT, WBC count and IL-6 levels(p<0.05). also WBC and IL-6 serums levels had significant correlation with base visfatin levels(p<0.05). also, a reverse correlation was found between Visfatin with LVEF and e' Lateral(p<0.05).
Conclusions: In conclusion, the current study revealed a positive association of Visfatin with EFT for the first time in AMI patients. In addition, these results represent a relationship between Visfatin and IL-6, WBC count, CK-MB, troponin-T, Gensini, and LVEF. Various factors such as atherosclerosis are involved in the pathogenesis of CAD. It has been suggested that an increase in Visfatin through inflammation may influence the process of atherosclerosis. Although this study does not show the causal link between Visfatin and CAD in patients with AMI, it suggests a role for Visfatin in these patient
Factors Affecting Unsuccessful Suicide Among Women Referred to Emergency Room of Ardabil City Hospital, Ardabil, Iran
Get PDFBackground: Along with the complexity of social communications, the rate of suicide is increasing, and according to studies, women commit suicide more than men. Various factors affect the occurrence of this phenomenon. The purpose of this study was to determine the factors affecting unsuccessful suicide among women referring to the emergency room of the hospital of Ardabil City, Iran.
Methods: This study was a descriptive cross-sectional study conducted on 236 women who had unsuccessful suicide. Questions were asked from patients with a checklist. This checklist has four parts. The first part relates to the patient’s personal information, and the second part relates to the patient’s previous history of mental, physical, and addiction problems. The third part asked about the causes of suicide and the fourth part related to the prior announcement. The collected data were analyzed by statistical methods in SPSS v. 18.
Results: The Mean±SD age of the women was 27.66±9.95 years, and the majority was between 15 and 20 years old. About 64% of women were housewives, and 37.7% had undergraduate education. Also, 36% had a positive history of psychological diseases, and 30.9% had a history of suicide. The most common causes of suicide among women were emotional problems (91.9%), family and marriage conflict (81.8%), spouse’s disregard (50.4%), and economic issues (44.5%).
Conclusion: The results showed that emotional problems and family and marital conflicts were common causes of suicide among women, and all women had at least one crucial reason for their suicide attempt
The role of miR‐34 in cancer drug resistance
No full textResistance to conventional chemotherapy remains a major cause of cancer relapse and cancer‐related deaths. Therefore, there is an urgent need to overcome resistance barriers. To improve cancer treatment approaches, it is critical to elucidate the basic mechanisms underlying drug resistance. Increasingly, the mechanisms involving micro‐RNAs (miRNAs) are studied because miRNAs are also considered practical therapeutic options due to high degrees of specificity, efficacy, and accuracy, as well as their ability to target multiple genes at the same time. Years of research have firmly established miR‐34 as a key tumor suppressor miRNA whose target genes are involved in drug resistance mechanisms. Indeed, numerous articles show that low levels of circulating miR‐34 or tumor‐specific miR‐34 expression are associated with poor response to chemotherapy. In addition, elevation of inherently low miR‐34 levels in resistant cancer cells effectively restores sensitivity to chemotherapeutic agents. Here, we review this literature, also highlighting some contradictory observations. In addition, we discuss the potential utility of miR‐34 expression as a predictive biomarker for chemotherapeutic drug response. Although caution needs to be exercised, miR‐34 is emerging as a biomarker that could improve cancer precision medicine
Restoration of miR‐330 expression suppresses lung cancer cell viability, proliferation, and migration
No full textLung cancer is one of the most common cancers and its incidence is rising around the world. Various studies suggest that miR-330 acts as a tumor-suppressor microRNA (miRNA) in different types of cancers, but precisely how has remained unclear. In this study, we investigate miR-330 expression in lung cancer patient samples, as well as in vitro, by studying how normalization of miR-330 expression affects lung cancer cellular phenotypes such as viability, apoptosis, proliferation, and migration. We establish that low miR-330 expression predicts poor lung cancer prognosis. Stable restoration of reduced miR-330 expression in lung cancer cells reduces cell viability, increases the fraction of apoptotic cells, causes G2/M cell cycle arrest, and inhibits cell migration. These findings are substantiated by increased mRNA and protein expression of markers for apoptosis via the intrinsic pathway, such as caspase 9, and decreased mRNA and protein expression of markers for cell migration, such as vimentin, C-X-C chemokine receptor type 4, and matrix metalloproteinase 9. We showed that reduced miR-330 expression predicts poor lung cancer survival and that stable restoration of miR-330 expression in lung cancer cells has a broad range of tumor-suppressive effects. This indicates that miR-330 is a promising candidate for miRNA replacement therapy for lung cancer patients