Ardabil University of Medical Sciences
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Evaluation of serum vitamin D level and its relationship with knee osteoarthritis stage in radiography in 2017-18
Get PDFBackground and Objective: Vitamin D is a steroid and lipid-soluble substance that in most cases exposure to sunlight, is the main source of this vitamin. Osteoarthrits is the most common joint affecting disease. Due to low studies on the relationship between the level of vitamin D and the progression of knee osteoarthritis in radiography, in Ardabil and Iran, and the increasing prevalence of osteoarthritis, our objective was to evaluate this issue.
Methods: 158 patients (79 cases and 79 controls) were selected among referral patients to rheumatology clinic of Imam Khomeini Hospital in Ardabil. The relevant questionnaire including demographic information were completed. Radiography in two anterior-posterior and side views of the knees in Imam Khomeini Hospital were performed with The serum level of 25-hydroxyvitamin D was also measured in the laboratory. Radiographic staging was also subdivided according to Kellgren-Lawrence criteria. The data were analyzed statistically.
Results: In the case group, 60.75% were female and 39.24% were male. In the control group, 58.22% were female and 41.77% were male. The mean age of the patients in the case group was 54.12 ± 4.67 years with a mean age of 67-44 years. The mean age of patients in the control group was 55.37 ± 5.12 years with an age range of 45-68 years. According to the results of this study, there was a significant relationship between patients with knee osteoarthritis based on Kellgren-Lawrence and serum vitamin D level.
Conclusion: Regarding the results of this study, it is recommended that medical doctors should consider vitamin D deficiency in patients with osteoarthritis, and in cases of low levels of it should take action to treat it
Inhibitory effect of gamma-secretase on the survival rate of dental pulp stem cells: An in vitro study
No full textObjective:To evaluate in vitro the effect of gamma-secretase inhibition on the survival of dental pulp stem cells. Material and Methods:Sound teeth have been used. Dental pulp stem cells were isolated by enzymatic digestion. An appropriate number of cells were treated with different concentrations of gamma secretase enzyme (DAPT) (1, 3, 6.25, 12.5, 25.5, 37.5, 50 and 100 µM). The metabolic activity of cells and the distribution of cells in different phages of cell cycle was evaluated by MTT assay and flow cytometry, respectively. Statistical analysis was made one-way ANOVA. Comparison was made between the groups on the level of p<0.05.Results:In low concentration of DAPT (1, 3, 6.25, 12.5) the growth rate of the cells increases, whereas in high concentration of DAPT (25.5, 37.5, 50, 100) can significantly reduce the viability of the treated cells. The results also indicate that DAPT can interrupt the cell cycle in G1 phase. Conclusion:The DAPT for dose-dependent survival rate of dental pulp stem cells and affect cell population increase in the G1 phase of the cell cycle
Optimization of Adsorption conditions of dye on low-cost adsorbent using Response Surface Methodology
Get PDFAim of study: The main environmental pollutants that are found in many industries, such as textiles are dyes. These dyes by entering the waters can cause toxicity, carcinogenicity and mutagenesis in human and aquatic species. Therefore, in this study Response surface method (RSM) was used to investigate the operational parameters and determine the optimum conditions for removal of acid blue 113 in the presence of bentonite. Methodology: To evaluate the removal acid blue 113 using the bentonite, the response surface method (RSM) was used based on the central composite design (CCD). The structure and morphology of bentonite absorbent were determined by XRD, FESEM, and XRF techniques. The effective parameters in the process such as: reaction time, initial pH, adsorbent dosage and initial concentration of dye were measured at three levels. Isotherms (Langmuir and Freunlich) and kinetic (pseudo first and second order) adsorption models were used for the analysis of this process. Results: The results of Physico chemical analysis confirmed the structure of bentonite. The proposed model (Quadratic) with high correlation coefficient (R 2 =0.9729 and R 2 Adj =0.92497) indicated that the experimental and predicted results were very consistent with the model. Finally, under optimal conditions
(initial dye concentration=32.31 mg/L, absorbent dose=0.53 g/L, reaction time= 82.98 min and pH=3.6) the removal efficiency was 98.62% with a desirability of 0.945 for acid blue 113. The results indicated adsorption process follows Freundlich isotherm (0.9216) and pseudo-second-order kinetic model (0.9985). Conclusion: The present study demonstrated that natural bentonite adsorbent has high ability in removal of acid blue 113. In addition, the central composite design can be used as an effective method to optimize dye adsorption from industrial wastewater containing high concentrations of dye
Myeloid-derived Suppressor Cells: important contributors to tumor progression and metastasis
No full textMyeloid-derived suppressor cells (MDSCs) are traditionally considered among the major components of the immunosuppressive tumor microenvironment. However, there is currently increasing evidence indicating that MDSCs in addition to suppression of immune surveillance is also involved in an array of non-immunological functions like augmenting metastatic potential of tumor cells. Indeed, MDSCs can promote metastasis in animal models and cancer patients through promoting premetastatic niche formation, tumor angiogenesis and invasion. Moreover, MDSC frequency and function have been associated with progressive disease and correlated with clinical outcome. This review will summarize and discusses the data demonstrating the role for MDSCs in tumor metastasis. This article is protected by copyright
Prenatal Diagnosis of Preeclampsia by Molecular of Circulating Corticotrophin-Releasing Hormone and Ep mRNA of Placental in Maternal Plasma
Get PDFObjective: Preeclampsia (PE) is a common disorder in pregnancy. Corticotrophin-releasing hormone (CRH)ats through vasodilatory effects. The aim of this study is diagnosis of PE by molecular of circulating CRH and Ep mRNA of placental in maternal plasma. Material and methods: The study was performed in early pregnant women with PE (n= 28) and normal pregnant women (n=30) as control group, blood samples was taken from each mother. Total RNA was isolated from harvested plasma synthesized from RNA samples using reverse transcription kit (Gen net bio) by the instructions of the manufacturer. Results: The Results indicated that expression of CRH was significantly different amongwomen suffered from preeclamptic compare of healthy pregnant women. Conclusion: This study demonstrated that thosemRNA concentrations were increased in the plasma of PE patients compared with those of normalsubjects and suggest the opportunity to gain furtherinsight into pathological pregnancy through mRNA measurementsin maternal plasma
Effect of miR-124-3p on neuronal differentiation of stem cells isolated from bulge region of hair follicle
Get PDFIntroduction:Hair follicle stem cells (HFSCs) are adult stem cells with high proliferative and developmental capacity in vitro that can be differentiated into neurons and glial cells.
microRNAs (miRNAs) are critical regulators of maintaining and changing cellular state during stem cell proliferation or differentiation. Distinct miRNAs regulate the proliferation and differentiation of HFSCs. Among the neural miRNAs, the function of miR-124 in the neural differentiation has been studied extensively. However, its exact role in the neuronal
differentiation of HFSCs has not been elucidated.
Material and Methods:HFSCs were isolated from mouse whisker follicles. miR-9, let-7b, and miR-124, Ptbp1, Sox9 , CCND1 (cyclinD1), NR2E1 (Tlx), RCOR2 ( CoREST), DLK1, Map2
and Rbfox3 (NeuN ) expression levels were detected by Real Time PCR (RT-PCR). The influence of miR-124 transfection was evaluated using immunostaining. Results:We demonstrated that miR-124 and let-7b expression levels were significantly increased after neural differentiation. DLK1,Map2 , Rbfox3 , CCND1 ، NR2E1 , RCOR2 ,Ptbp1 and Sox9 were identified as targets of miR-124 in neuronal differentiation of the HFSCs.During neural differentiation and miR-124 mimicking, Ptbp1 , Sox9 , CCND1 ، NR2E1 , RCOR2 levels were decreased and increased the level of expression of DLK1, Map2 and Rbfox3 genes in HFSCs. Moreover, miR-124 overexpression increased MAP2 (58.43±11.26) and NeuN (48.34±11.15) proteins expression. Conclusion:The results demonstrated that miR-124 may promote differentiation of HFSCs into neuronal cells by targeting non-neural genes Sox9 , Ptbp1,CCND1 ، NR2E1 and RCOR2. Key words: Hair follicle stem cells, Neural differentiation, miR-124, Sox9, Ptbp1, CCND1 ، NR2E1 , RCOR2,DLK1,Map2,Rbfox
Vitamin D supplements’ effect on expediting the weaning process in patients with the stroke
Get PDFFaster weaning from mechanical ventilation in ICU reduces the side effects of long-term mechanical ventilation and the cost of admission to the hospital. The objective of this study was to investigate the effect of vitamin D supplement on the facilitation of weaning in the stroke patients. In this study 99 stroke patients who were admitted to the ICU and intubated due to a fall in their level of consciousness entered the study. The level of 25 (oh) D was assessed in all of the patients and then they were divided into two different groups based on their level of vitamin D. Those included in group A (33 patients) had the vitamin D level of higher than 20 ng/ml and those in the other group (66 patients) had the vitamin D level of lower than 20 ng/ml who themselves were further divided into the two groups of B and C randomly (each consisting of 33 patients). The patients in group B received intramuscular vitamin D3 and tablet of calcium D. In the meantime, placebo was administered to the patients in group C. At the end of the intervention the mean level of vitamin D was 28.04±10.3 ng/ml in group A, 8.85±2.3 ng/ml in group B, and 10.52±4.81 ng/ml in group C. The mean durations of intubation for patients in groups A, B, and C were 13.81 days, 15.96 days, and 15 days, respectively. The patients in group A had the least duration of intubation. The three groups were significantly different as regards the duration of patients’ intubation (p0.05). The results of this study indicate the effect of vitamin D on accelerating the weaning of patients in the intensive care unit in stroke patients
Frequency of mecA, pvl, arcA, speG genes in Staphylococcus aureus isolated from Burn Wounds in Tabriz, Iran
Get PDFBackground: Burn wounds provide a proper position for bacterial growth and are more persistent richer sources of infection than other wounds. Staphylococcus aureus (S. aureus) is one of the most commonly isolated pathogens in burn wound infections. Methods: One hundred S. aureus isolates were collected from burn wound infections. Identification of S. aureus was performed by phenotypic and molecular methods. The Kirby-Bauer and agar dilution methods were performed for determination of antibiotic susceptibility patterns. Polymerase chain reaction (PCR) was carried out for detection of mecA, pvl, nuc, arcA and speG genes. Results: Methicillin resistant S. aureus (MRSA) was detected in 21 % of isolates by mecA gene PCR and phenotypic methods. All isolates were negative for arcA and speG genes. All of isolates were susceptible to linezolid, vancomycin and mupirocin. The resistance rate to erythromycin and clindamycin were high. Conclusion: Our findings showed that S. aureus isolates have high-level resistance to antimicrobial agents and that appropriate antibiotic therapy, based on the antibiotic susceptibility pattern, is essential to ensure a good result. Thus, strict consideration for S. aureus infection and proper usage of antibiotic policy are recommended in decreasing the incidence and occurrence of multidrug resistant S. aureus infections in burn wound infections in this hospital
