Ardabil University of Medical Sciences
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Familial Mediterranean Fever Gene Mutations and Gout as an AutoInflammatory Arthropathy
Get PDFIntroduction: The auto-inflammatory diseases (AID) are a heterogeneous group of multi-system disorders of innate immunity dysregulation. MEFV gene has major role in AID. Aim: The aim of this study is to investigate the frequency of MEFV variant alleles in gout patients as an AID and their genotype-phenotype relationship. Methods: Total amount of 224 of the healthy people as a control group (113 male and 111 female) and 24 gouty arthritis patients (20 male and 4 female) entered this study. Blood samples screened for the 12 common pathogenic mutations according to manufacturerÂ’s instructions. Results: The mean age of patients was 54 years. MTP joint was the most involved joint (91.66%). Mutations were shown in 5 patients (20.83%) that were not different from healthy population (25%). Five patients carry one mutated MEFV allele, E148Q in 4 patients and V726A in 1 patient. Control group showed 25% mutations as E148Q (18.3%), P369S (3.1%), V726A (2.2%), A744S (1.3%) respectively. The most common mutation detected in patients was E148Q (16.66%) and all of them were males. No significant and meaningful associations were detected between the MEFV gene mutations and gouty arthritis patients. Conclusion: There was not any correlation between MEFV gene mutations carriage with age, sex, the number of joint involvement and the course of disease in gouty arthritis. MEFV gene mutations were more frequent in men than women, but this is not statistically significant
Predictors of Fear of Childbirth in the Primiparous Women in Ardabil-Iran
Get PDFIntroduction: Fear Of Childbirth (FOC) is the main factor
leading to elective and emergency cesarean section. FOC and
prenatal depression affect the women and fetus health status
and it is associated with negative neonatal outcomes during pregnancy. Aim: To determine the predictors of FOC in pregnant women. Materials and Methods: This cross-sectional study was carried out on 230 primiparous women between 24th and 30th weeks of pregnancy who referred to 17 urban healthcare centres of Ardabil-Iran.
Six centres of 17 urban healthcare centres were
randomly selected using a cluster sampling method. A list
of the women who met the inclusion criteria and willing to
participate in this study was prepared. The socio-demographi
questionnaire, Edinburgh Postnatal Depression Scale (EPDS),
and Wijma Delivery Expectancy Questionnaire (WDEQ-A) were
used to collect data through in-person interviews. The statistical analyses were performed with the Pearson’s correlation test and the general linear model. Results: The mean (SD) scores of the WDEQ-A and the EPDS were 58.4 (26.1) and 8.9 (5.1), respectively. More than one third of women (38.6%) had high FOC and about one third (34.1%) had moderate FOC. On the base of the Pearson’s correlation test, there was a significant correlation between FOC and depression (p<0.001, r=0.51). Moreover, based on the general linear model, the variables of depression, sufficiency of family income and non-male fetal gender were predictors of FOC and they explained 28.5% of the variance in childbirth fear.
Conclusion: The present results showed that the pregnancy
depression correlated with FOC. Therefore, identifying and
treating depressed pregnant women can decrease FOC and
its complication on the women and neonates. It may be also
effective in reducing unnecessary cesarean sections
Evaluation of the Relationship between Serum Vitamin D Level and Early Onset of Sepsis
Get PDFBackground: Neonatal sepsis is a critical condition caused by a generalized bacterial infection and is one of
the most common causes of neonatal mortality and morbidity in the first month of life. Vitamin D is a steroid
hormone that has numerous roles in the immune system and reduces inflammation. The purpose of this study
was to evaluation of the relationship between serum vitamin D level and neonatal early onset of sepsis (EOS).
Materials and Methods: In this case-control study, 43 newborns with EOS selected in the case group and 43
matched healthy children were selected as the control group. The demographic data such as gestational age,
delivery method, birth weight and the results of blood and biochemical tests were collected and then analyzed
by statistical methods in SPSS version 21.
Results: In the case group, the mean age was 4.93 days and 72.1% were boy. The most common complaints in
neonates were restless with 74.71%, jaundice with 67.44% and fever with 62.7%. The average age of mothers
with a septic newborns was 26.83 years and 23.3% of mothers had received vitamin D supplementation during
pregnancy. Dairy consumption in studied mothers was low and the majority of them had exposure with sunlight
less than 15 minutes. A high percentage of infants (94.2%) had severe to moderate vitamin D deficiency that
in septic neonates was higher than healthy neonates but no significant. Also, there was no significant relation
between the level of vitamin D and incidence of sepsis.
Conclusion: The results showed that there is no significant difference between vitamin D levels in mothers and their neonates but in the case group, there was a significant correlation between maternal and neonatal serum
vitamin D level
Molecular Docking Study of Anti-viral FDA-approved Drugs as Novel Entry and Replication Ebola Viral Inhibitors
Get PDFBackground & Objective: Because of the reported high ability of virulence and the lack of appropriate drug of Ebola virus during the last decades, many investigations have been
accomplished regarding discovery and the introduction of anti-Ebola drugs. The aim of this research was the bioinformatical study of entry and replication of Ebola viral inhibition by drug repurposing.
Materials & Methods: It is a descriptive-analytic study. In order to investigate the mode of interaction of the compounds with GP and VP40 binding sites, the chemical structures of allcompounds were designed using ChemDraw program, then were transferred into Hyperchem software for energy minimization. Molecular docking simulation was accomplished using AutoDock
4.2 program.
Results: Docking results revealed the hydrophobic, hydrogen bond, π-π and π-cation contacts were involved in the drug-protein interactions. Among all the studied drugs, the best docking results were related to Amodiaquine and Diphenoxylate drugs displayed. Actually, this compounds had the most negative ΔGbinding that indicated suitable modes and favorable interactions with the amino acid residues at the binding site of GP and VP40. The weakest docking results were exhibited for Dirithromycin and Erythromycin drugs due to the high hydrophilic character of them. In general, the
presence of hydrophobic portions, tertiary amines, and optimal hydrogen bonds increases the strength of anti-Ebola medications. Conclusion: According to the results of the molecular docking, the entire FDA-approved drugs revealed a good inhibition effect on entry and replication Ebola viral
Co-administration and Evaluation of Immune Responses of Three DNA Vaccines Encoding Immunogenic Antigens from Mycobacterium tuberculosis
Get PDFBackground: Ineffectiveness of BCG vaccine in controlling tuberculosis (TB) and co-infection of TB and HIV have turned TB into a serious global threat. Therefore, the development of an alternative vaccine to BCG and/or antimycobacterial drugs is an urgent need. Here, three chimeric DNA constructs consisting of Mtb32C-HBHA, Ag85a-Tb10.4, and Ag85a-cfp10 made in our previous studies were co-administered to BALB/c mice to evaluate their immune responses using a prime-boost regimen in which the animals were first immunized with BCG and then administered with DNA vaccines.
Methods: In order to evaluate the immunogenicity of three DNA constructs, the levels of several immunomodulatory cytokines were measured in vaccinated mice. Thirty female BALB/c mice were divided into the following groups (n = 10): control (receiving pcDNA 3.1+ intramuscularly), vaccine (receiving recombinant vectors intramuscularly), and vaccine-BCG (receiving BCG subcutaneously followed by recombinant vectors intramuscularly).
Results: The levels of IL-4, IL-12, TGF-β, IFN-γ, and IL-10 were higher in the immunized groups than in the control group (P 0.05). Conclusions: Our results proved that using a chimeric DNA vaccine as a booster in the prime-boost strategy could significantly enhance the efficacy of BCG. This study suggests that the use of such DNA vaccines encoding mycobacterial immunogenic antigens as boosters enhances the efficacy of BCG
Social well-being of Iranian retired men of the armed forces and their wives
No full textAbstract
Background Social well-being is a major component of
health, which was somehow neglected until the last few
decades. The aim of this study was to determine the state
of social well-being of Iranian retired men of the armed
forces and their wives in the metropolis of Tehran.
Methods This is a cross-sectional study which was
conducted in Tehran, capital of Iran, in 2016. Three
hundred retired men of the armed forces and their wives
were selected by random sampling. We used the Social
Well-Being Scale to collect data. We assessed its internal
consistency and validity with Cronbach’s alpha and
exploratory factor analysis before using it. We used SPSS
V.24 software to analyse the data via χ2, Pearson’s correlation,
t-test and analysis of variance.
Results The mean age was 66.39±6.98 years; 60%
were male. The findings revealed that retired men of the
armed forces and their wives report a relatively modest
degree of well-being. Social well-being of the participants
was significantly related to their marital status (p=0.03),
education (p=0.03), employment status (p<0.01),
housing status (p<0.01), financial status (p<0.01) and
health status (p<0.01).
Conclusion The social well-being of retired men of the
armed forces and their wives was moderate and was not
different from each other, and it was related to housing
ownership, employment, self-rated socioeconomic condition,
education, good health and married status
Skin Sensitization Study of Honey Bee (Apis mellifera) and Yellow Jacket (Vespula sp.) venom on Rabbits
Get PDFBee venom is a special and exceptional defensive weapon in the animal world. Bee venom is also the first and most important defense to defend the bee colony. Bee venom is a different combination of widest variety of chemical sub-stances that protect the bees against a wide range of enemies (1). The most important allergen in bee venom composition is phospholipase A2, which is a glycoprotein with 134 amino acid resi-dues (2). The main and great allergens in vespid venoms are phospholipase A1 (Ves v 1), hyalu-ronidase (Ves v 2), and antigen 5 (Ves v 5). Phospholipase A1 include 6–14% of the total dry weight of vespid venom (3). Antigen 5, is a con-siderable allergen in all vespid venoms (4). Skin sensitization potential plays an important part of any toxicology program for new consumer prod-ucts (5)
Comparing Perceived Dignity and Privacy in Patients Hospitalized in Internal and Surgical Wards of Ardabil Educational Medical Centers
Get PDFThe present study aimed to compare perceived dignity and privacy of patients admitted to internal and surgical wards of medical educational centers in Ardabil, Iran. This is a cross-sectional descriptive study. Stratified random sampling was performed in the internal and surgical wards of hospitals affiliated to Ardabil medical educational centers (Imam Khomeini, Alavi and Fatemi). The study population included 279 patients admitted to the internal and surgical wards of the hospitals. The statistical sample was computed using the Cochran formula (n = 384). Patients' information was collected using three questionnaires including Patient Dignity Inventory (PDI), Patient Privacy Questionnaire and demographic questionnaire. The reliability of Patient Privacy Questionnaire (0.81) and Patient Dignity Inventory (0.87) was confirmed using Cronbach's alpha. The results showed that the patient’s perception of dignity is higher than the determined criterion and perceived dignity of patients in the surgical ward is at the level of the determined criterion. In addition, perceived privacy of patients is higher than the determined criterion; the perceived privacy of patients is higher than the determined criterion and perceived privacy of patients in internal wards is higher than the surgical wards. Further, Fatemi hospital, with a mean score of 60.67, was classified in one category, which has a significant difference (0.05) with two Alawi and Imam Khomeini hospitals in terms of patient’s perceived dignity. Furthermore, Fatemi Hospital, with an average privacy of 95.21%, was classified alone in one category, which has a significant difference at the level of 0.05 with both Alawi and Imam Khomeini hospitals in terms of patient’s perceived privacy. The results indicated that the improved dignity of the patients has led to increased level of their privacy