Universiti Malaysia Terengganu

Universiti Malaysia Terengganu
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    Effects of Low Salinity on Growth, Digestive Enzyme Activity, Antioxidant and Immune Status, and the Microbial Community of Litopenaeus vannamei in Biofloc Technology Aquaculture Systems

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    Biofloc technology (BFT) culture systems based on low salinity can meet the needs of shrimp growth and environmental protection, thus having potential application. To evaluate the effects of BFT on water quality, the microbial community, growth, digestive enzyme activity, and antioxidant and immune status of Litopenaeus vannamei under low salinity conditions, a four-week experiment was conducted in a BFT culture system (C/N ratio of 12: 1) with three salinity conditions: 5.0? (S5 group), 10.0? (S10 group), and 15.0?. The results showed that water quality parameters were all within the range suitable for the culture of L. vannamei. There were no significant differences in growth parameters such as final weight or specific growth rate. Muscle moisture, crude protein, and crude lipid contents of L. vannamei did not differ among groups (p > 0.05). In addition, intestinal amylase and trypsin activities in the S5 group significantly increased (p < 0.05). In the S15 group, the superoxide dismutase activity and total antioxidant capacity in the serum and hepatopancreas of L. vannamei, as well as serum catalase, acid phosphatase, and alkaline phosphatase activities, markedly increased (p < 0.05). The microbial diversity (Shannon and Simpson indices) and richness (Chao1 and ACE indices) were higher in the S5 group than in the S15 group. Our findings indicated that although the shrimp in BFT systems with a salinity of 5?had lower antioxidant and immune levels, the digestive enzyme activity as well as the gut microbial diversity and richness improved compared to other groups, suggesting the possibility of culturing L. vannamei in low-saline areas with BFT systems

    Cephalopod ontogeny and life cycle patterns

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    Life cycle definitions provide the background for conceptualizing meaningful questions to address the mechanisms that generate different life cycle patterns. This review provides explicit definitions and explanations of the steps in a cephalopod life cycle, from fertilization to death. Each large step, or phase, is characterized by a particular developmental process and morphology. Each phase is composed of smaller developmentally distinct steps, or stages. The cephalopod life cycle is comprised of all or some of the following phases: Embryonic, Paralarval, Juvenile, Subadult, Adult and Senescent, and each life cycle is taxon-specific. All cephalopods have direct development and maintain a consistent body plan throughout ontogeny (i.e., no true larval phase and no metamorphosis). Most cephalopods have a life cycle marked by a long early life and a short adult life followed by senescence. Cephalopods have two developmental modes: they produce either small planktonic hatchlings as paralarvae, or large hatchlings as juveniles. All cephalopods go through a Hatchling stage soon after eclosion during which they rely on two modes of nutrition: endogenous (yolk) and exogenous (prey). Many cephalopods with planktonic paralarvae will become benthic early in their life cycle during their Settlement stage or remain pelagic during their Metapelagic stage. Juvenile growth is fast and ontogenetic changes (outside of gonadal maturation) generally cease at the end of the Juvenile phase. The Subadult phase begins when the definitive adult morphology (except for size and body proportions) is acquired (e.g., full complement of photophores). Sexual organs undergo most of their development during the Subadult phase. The Adult phase starts with spawning competency and concludes when gonads are spent. The Senescent phase begins with spent gonads and ends with death. Using this new terminology, we examine the patterns of cephalopod life cycles and find that there are four main patterns based on the presence of a Paralarval phase and the habitat occupied by each phase: Holopelagic (all phases are pelagic), Holobenthic (all phases are benthic), Merobenthic and Meropelagic (phases alternate between benthic and pelagic environments). In these two last patterns, the main difference is the presence of a Paralarval phase in Merobenthic species. The definitions and terminology proposed here provide a unifying framework for future ecological, evolutionary and life cycles research on cephalopods

    Broadband Scattering Characteristic Quantization Technique for Single Fish Based on a Split-Beam Echosounder

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    The utilization of broadband quantization data increases the possibility of practical applications for fish target recognition based on the acoustic scattering theory. However, the quantification of broadband data is more complex than that of narrowband systems, requiring consideration of the broadband characteristics of split-beam transducers, seawater absorption, and circuits. This paper elucidates the scatterer acoustic field equation, transducer power equation, and signal processing flow in split-beam broadband quantization technology for engineering applications. A broadband calculation model based on transducer parameters is proposed to enhance the generalization ability of broadband quantization technology to different types of sonar. The classical echo integration method is combined with Fourier transform to meet the requirements of target strength (TS) estimation under broadband signals. This paper includes a series of experiments to prove the rationality and effectiveness of the method. The results demonstrate that the provided calculation model can more accurately reflect the backscattering characteristics of the scatterer, but certain errors remain. This article analyzes the sources of errors and validates the effectiveness of the new TS calculation metho

    PROFICIO UMTMamm

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    Length-weight relationships of three endemic fish species of the Arabian Peninsula

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    Length-weight relationships (LWRs) were evaluated for three endemic fish species collected from the north and south of inland waters of Oman in 2022 including Garra sindhae, G. smartae, and Oxyurichthys omanensis by using foldable shrimp and crab fishing traps (mesh size of 3*3mm) and hand nets (mesh size of 3*3mm). The parameter of b for all three species was between the estimated ranges of 2.5-3.5 as proposed for different other fish species, and there was high and significant coefficient of determination for all three species

    Effect of Tetraselmis suecica microalgae isolated from the Persian Gulf on the expression level of AKT/mTOR genes in the liver cancer cell line, Huh7

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    The medicinal and therapeutic properties of microalgae, especially their anti-cancer properties in modulating cellular mechanisms such as cytotoxicity, reducing tumor cell invasion, and increasing apoptosis, have drawn attention to their therapeutic application in cancer. However, there are few reports on the effects of Tetraselmis suecica microalgae on liver cancer. As the aim of this study, after ethanolic extracting from T. suecica, and IC50 determination, the effect of T. suecica microalgae extract on cytotoxicity and apoptosis of Huh7 cells was investigated by MTT and Annexin V/PI staining, respectively. In addition, the expression of AKT/mTOR genes was measured by real-time PCR test. Based on the results, ethanolic extract of T. suecica to the Huh7 cell line significantly decreased the expression of AKT and mTOR genes compared to the control group. The vitality of cancer cells in concentrations of 500 and 1000 ?g/ml of T. suecica extract in a 48-hour culture, decreased significantly. In addition, in the 72-hour treatment, a significant decrease in cell viability was observed in the concentrations of 250, 500, and 1000 ?g/ml. According to the results, T. suecica microalgae can offer good benefits as valuable natural materials for the pharmaceutical industry, especially anticancer drugs

    Experimental study on the effect of sound stimulation on hearing and behavior of juvenile black rockfish (Sebastes schlegelii)

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    Assessing the potential impacts of wind farm noise on fish is a crucial aspect of Environmental Impact Assessment (EIA) studies. There is increasing evidence of disturbances and effects on hearing and behavior in animals. The black rockfish (Sebastes schlegelii) is a commercially valuable rocky reef fish native to East Asia. However, empirical studies that measure the actual consequences are lacking. In this study, we used auditory evoked potentials (AEP) to assess the effects of dominant frequency noise emitted by offshore wind farms on the auditory sensitivity, hearing threshold, swimming, and feeding behavior of juvenile black rockfish. The experimental findings revealed that the most sensitive sound frequency was 200 Hz, with the lowest hearing threshold recorded at 86.4 ? 3.4 dB re 1 mPa. Following 3 and 7 days of exposure to 200 Hz noise at 110 dB, threshold shifts in black rockfish reached 19.0 dB and 13.3 dB, respectively. During the subsequent recovery phase, these shifts decreased to approximately 9.8 dB after 3 days, respectively. The noise-exposed group exhibited higher swimming duration, moving distance, and caudal fin swing frequency compared to the control group without noise exposure. Furthermore, noise prolonged the feeding rate of black rockfish. Our findings provide the first evidence of noiseinduced temporary threshold shift and behavioral disturbances in juvenile black rockfish, implying potential fitness consequences associated with noise pollutant

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