Universiti Malaysia Terengganu

Universiti Malaysia Terengganu
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    PEMBANGUNAN PROTOKOL KRIOAWETAN SPERMA UNTUK PATIN BUAH, Pangasius nasutus (Bleeker,1863)

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    Molecular Characterization of the Actin Gene and 5? Flanking Sequence from Brown Macroalga Saccharina japonica (Laminariales, Phaeophyta)

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    The brown macroalga Saccharina japonica (Laminariales, Phaeophyta) is the most productive cultured seaweed in the world. In order to improve the biosafety of transgenic kelp, it is necessary to develop endogenous constitutive promoters, replacing those of virus origin. In this study, the housekeeping actin gene from S. japonica (SjACT) was found to contain three exons and two introns, representing a unique actin gene structure pattern in brown algae. Additionally, the 5? upstream region was obtained using genome walking, and fused to the reporter gene lacZ or EGFP to construct promoter-detective vectors. Using an established genetic transformation system, kelps in different life-cycle stages were transformed. The detection results showed that, in the diploid sporophyte stage, the transient expression from the lacZ gene could be observed in the frond, stipe, or holdfast of kelps, indicating a manner of being non-tissue-specific. And, in the haploid gametophyte stage of S. japonica, the fluorescence of the expressed EGFP were detected in vivo in gametophyte cells of both genders. These results indicate that the promoter of the SjACT gene (pSjACT) functions in a constitutive manner and is expected to be a key endogenous element in the genetic manipulation of kelps

    Blueprint for Blue Carbon: Lessons from Seychelles for Small Island States

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    Blue carbon has been proposed as a nature-based solution for climate change mitigation; however, a limited number of published works and data and knowledge gaps hinder the development of small island developing states? (SIDS) national blue carbon resources globally. This paper reviews the blue carbon ecosystems of Seychelles as a case study in the context of SIDS, comparing estimations by the Blue Carbon Lab and recent blue carbon (mangrove and seagrass) evaluations submitted to the Seychelles national government. Mangroves (2195 ha, 80% in Aldabra Atoll) and seagrasses (142,065 ha) dominate in Seychelles, with coral reefs having the potential for carbon sequestration (169,000 ha). Seychelles is on track to protecting its blue carbon, but these systems are threatened by rising sea levels, coastal squeeze, erosion, severe storms, and human activities. The importance of carbon inventories, accounting institutions, and continuous monitoring of blue carbon systems is discussed. Blue accounting is necessary for accurate accounting of carbon sequestration and carbon storage, generating carbon credits, and representing impactful reductions in greenhouse gases for NDCs. Challenges and opportunities include policy legislation regarding ownership rights, accreditation and certification for carbon credits, sustainable financing mechanisms like natural asset companies and blue tokens, local engagement for long-term success, and carbon market dynamics following COP27. The restoration and regulation of blue carbon resources for optimal ecosystem services delivery, carbon inventories, and blue carbon policy are recommended development priorities. Blue carbon ecosystems have the potential to contribute to NDCs of SIDS while simultaneously offering sustainable development pathways for local communities through the multiple ecosystem services they provide

    Assessment of Ecosystem Characteristics and Fishery Carbon Sink Potential of Qianxiahu Reservoir Based on Trophic Level and Carbon Content Methods

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    Optimizing biological carbon sequestration has become a primary strategy in global lowcarbon- emission initiatives. Freshwater fisheries in reservoirs play an important role in aquatic biological carbon sequestration. However, a standard method for evaluating the carbon sink capacity of inland fisheries has not been developed. Therefore, this study aimed to assess and compare the carbon sequestration potential of the Qianxiahu Reservoir?s fisheries using the trophic level and mass-balance methodologies. The Ecopath model was employed to determine the trophic levels of aquatic organisms within the Qianxiahu Reservoir ecosystem, with input parameters sourced from in situ surveys and the literature on reservoirs. The model includes 21 functional groups, with trophic levels ranging from 1.000 to 3.281. The key species identified are silver carp, bighead carp, and crucian carp. The indices of Finn?s cycling index (FCI), connectivity index (CI), system omnivory index (SOI), and total primary production/total respiration (TPP/TR) for the Qianxiahu Reservoir are 11.35, 0.27, 0.196, and 1.540, respectively. These values indicate a high degree of material recycling and complex interconnections among functional groups. The fishery carbon sink potential of the Qianxiahu Reservoir, calculated using the trophic level and carbon content methods, yielded values of 261.8362 tons/km2 and 66.6818 tons/km2, respectively. The trophic level method showed a notable increase of 195,1544 tons/km2 compared to the carbon content method, underscoring significant differences in results between the two methods. The study concludes with recommendations for research on methods to assess the carbon sink capacity of freshwater fisheries, aiming to establish a scientific framework for this evaluation

    A COMPREHENSIVE ANALYSIS OF FLOODING MECHANISMS ALONG CRASNA RIVER

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    cross the world best practice, the flood hazard and risk maps are core instruments used for the development and implementation of flood management policies. These maps sit as base information for developing flood management strategies and plans, designing of new flood defence schemes and assessment of the existing flood scheme performance, elaboration of emergency and action plans, territorial planning (zoning and permissions), land use control, climate changes impacts evaluation, insurance industry etc. Romania did remarkable steps in developing high quality flood hazard and risk maps as core action for an integrated flood risk management. In the second cycle of Floods Directive 2007/60/EC implementation, Romania developed and reported to European Commission (EC) the hazard and risk maps for 526 APSFRs (Areas with Potential Significant Flood Risk). The hazard maps built in second cycle are the result of detailed models developed based on high resolution Lidar (0.5m), calibrated hydrological data and advanced modelling technics. Crasna River is one of the watercourses from the Somes-Tisa Basin which benefited from advanced 2D hydraulic modelling having in-place all the existing infrastructure and which has been well - calibrated on the flood event recorded in 2015. The calibration of the model on 2015 flood event was performed both for flows and levels. The article aims to explicitly present the performance of the existing defence system of Crasna river during extreme flood events with different magnitudes having in hand these high-quality hydraulic model and hazard maps

    Continuous Field Determination and Ecological Risk Assessment of Pb in the Yellow Sea of China

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    Field determination and ecological risk assessment of dissolved lead (Pb) were performed at two Yellow Sea sites in China using a continuous automated electrochemical system (CAEDS). This CAEDS instrument includes an automatic triple filter sampler and an electrochemical detection water quality analyzer, which might be operated automatically four times daily. The dissolved Pb concentrations varied from 0.29 to 1.57 ?g/L in the South Yellow Sea over 16 days and from 0.32 to 2.28 ?g/L in the North Yellow Sea over 13 days. During the typhoon and algal bloom periods, the Pb concentration was as high as ten times greater than usual. According to the calculation of contamination factors (Cf) and subsequent analysis, seawater quality was classified as Grade II. Through species sensitivity distribution (SSD) method experiments and ecological risk analysis, an average risk quotient (RQ) below 1 for both areas was obtained, indicating a low-to-moderate ecological risk. This system will be helpful for Pb monitoring and assessment in seawater and contribute to the biogeochemical cycling study of Pb

    Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge ofMactra veneriformis with Different Shell Colors

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    Mactra veneriformis is highly susceptible to ocean acidification (OA) due to its low shell hardness during its rapid growth period. In this study, oxygen consumption rate, ammonia excretion rate, and transcriptome sequencing of mantle tissue analyses were conducted in white and purple shell-color populations of M. veneriformis under OA stress (pH = 7.6). The findings indicated a significant rise in oxygen consumption rates and ammonia excretion rates following acidification in both the two shell colors, while the clams with purple color showed comparatively lower basal metabolic levels. Transcriptomic analyses demonstrated the expression of key genes related to fatty acid synthesis were significantly inhibited, whereas genes involved in calcification, osmoregulation, and immune response were upregulated under OA exposure in the two shell-color groups. However, some genes such as CA and HSP showed a population-specific response between the two shell-color populations. KEGG enrichment analysis revealed that the MAPK signaling pathway and protein processing in the endoplasmic reticulum were significantly enriched in the two acidification groups. This study provides valuable insights into the response of M. veneriformis to OA stress and also helps to predict the future breeding of valuable strains of M. veneriformis

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