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Can Chaperones Promote Protein Splicing?
No full textInteins are a mobile genetic element hypothesized to function as regulatory molecules in microbes. They bear a homing endonuclease domain for genome traversal, as well as specific residues allowing for protein splicing. In the protein splicing reaction, the intein is removed and the flanking residues, termed exteins, are ligated together with a peptide bond to form a functional host protein. Due to their interruption prior to splicing, inteins prevent a host protein from functioning until the intein ligates the two exteins. To measure factors that regulate protein splicing in vivo, an intein-based biosensor based on kanamycin resistance (Kanamycin Intein Splicing Reporter) was previously developed. In this work, we use our KISR reporter to examine whether chaperones - proteins that assist in the folding of other proteins – can promote the protein splicing reaction. Specifically, we test whether expression of the chaperone GroEL can promote protein splicing within the cellular environment. Indeed, our results demonstrate increased protein splicing increased in the presence of both the KISR and GroEL, but not in the presence of the kanamycin resistance protein lacking the intein and GroEL. Our findings have implications for the potential regulation of protein splicing in nature, as well as on the use of inteins in protein engineering
Carroll, Base Photographic Laboratory
No full texthttps://digitalcommons.murraystate.edu/julian-carroll-coll/1001/thumbnail.jp
Julian Carroll and Associate
No full texthttps://digitalcommons.murraystate.edu/julian-carroll-coll/1003/thumbnail.jp
Julian Carroll Inauguration
No full texthttps://digitalcommons.murraystate.edu/julian-carroll-coll/1005/thumbnail.jp
Julian Carroll House of Representatives Acceptance Letter 9
No full texthttps://digitalcommons.murraystate.edu/julian-carroll-coll/1012/thumbnail.jp
Julian Carroll Swearing in Ceremony 2
No full texthttps://digitalcommons.murraystate.edu/julian-carroll-coll/1029/thumbnail.jp
Julian Carroll and Associates
No full texthttps://digitalcommons.murraystate.edu/julian-carroll-coll/1044/thumbnail.jp
The Deadliest Mental Disorder: Borderline Personality Disorder
No full textBorderline personality disorder is the most fatal mental disorder in the DSM-5. The difference between life and death could be a mental health professional who is knowledgeable in BPD and its treatments. To better understand what BPD is and how to treat it, it’s paramount to know how the diagnosis of BPD came about and how it originated from the diagnosis of psychosis and neurosis. Mental health professionals also need to be educated in personality disorders as a whole and what symptoms meet the criteria for a personality disorder. The DSM-5-TR is an excellent resource for those in the mental health profession as it gives information and criteria for mental disorders. They can use the DSM-5-TR as a reference for what borderline personality disorder is and how to diagnose the disorder. In the diagnosis of BPD, there are 9 criteria, and an individual needs to show 5 of the 9 to be eligible for a diagnosis of BPD. When researching the causes of borderline personality disorder, we can see how the development of BPD progresses and how mental health professionals can gear treatment towards the individual. Once a diagnosis of BPD is established, psychotherapy treatment can begin, and those with BPD can begin their journey to a healthier life. Lastly, by bringing awareness to the stigma that those with BPD face, we can begin to dismantle the barriers facing those seeking treatment. This research concludes that the awareness and education of borderline personality is necessary for those in the mental health profession.
Keywords: borderline personality disorder, personality disorders, mental health, psychosis, neurosis, the borderline group, BPD treatment, BPD stigm
How Has NIL Impacted College Athletes
No full textSince the NCAA’s Name, Image, and Likeness (NIL) policy shift in 2021, college football has undergone one of the most transformative changes in its history. This essay explores the various ways NIL has impacted college football athletes, both on and off the field. At its core, the policy has opened doors for athletes to gain financial independence, turning their personal brands into real-world assets. For many, this newly found earning potential has provided critical support for themselves and their families, while also teaching them business skills, financial literacy, and professional responsibility. Yet, NIL has also introduced a slight imbalance the college scene itself. The paper examines the that have emerged between high profile stars and lesser known players, as well as between athletes at major programs versus those at smaller schools. It also addresses the growing role of social media platforms like Instagram and TikTok, which have become key tools for athletes to increase their marketability but have also added pressure to perform both athletically and digitally. Additionally, the essay highlights how NIL opportunities are beginning to influence recruiting decisions, transfer choices, and even athlete retention, reshaping the competitive balance within college football. While NIL has not been without its challenges including regulatory inconsistencies and ethical concerns the overall picture painted in this essay suggests that the policy has empowered athletes in unprecedented ways. By tracing these developments, the work ultimately argues that NIL is not just a financial shift but a cultural and structural evolution in college football, one that is preparing athletes for life far beyond their playing days
