1025 research outputs found
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Interrogating the Functions of the Histone H2A Repression Domain in Relation to Cell-Cycle Progression in \u3cem\u3eSaccharomyces\u3c/em\u3e \u3cem\u3ecerevisiae\u3c/em\u3e
Full text linkIn eukaryotic cells, DNA is wrapped around histone proteins to form the basic repeating unit of chromatin, the nucleosome core particle. Most nucleosomes consist of two copies each of histones: H2A and H2B in a heterodimer and histones H3 and H4 in a heterotetramer. Because of their intimate association with DNA, histones regulate all DNA-templated processes such as transcription, DNA replication, and DNA damage response and repair. Several studies have illustrated the importance of a small portion of the histone H2A amino terminal domain which regulates global transcription. This domain is responsible for the repression of ~4% of the yeast genome. This domain, termed the histone H2A Repression (H.A.R.) domain, consists of a small, conserved portion (amino acids 16-20) of histone H2A. The H.A.R. domain has also been shown to be an important regulator of DNA damage response and repair. In this study, we find that deletion of the H.A.R. domain leads to a defect in cell proliferation. Indeed, deletion of the domain leads to a marked sensitivity to hydroxyurea, indicating a defect in either DNA damage response or DNA replication machinery. Intriguingly, we find that the RNR2 gene (which regulates the transition of nucleoside triphosphates into deoxynucleotide triphosphates) is down regulated in these mutants. Finally, we find that deletion of the H.A.R. domain leads to an abnormal number of cells that show an apparent delay in the transition out of S-phase in the cell cycle. Taken together, these data points illustrate the importance of this domain in proper progression through the cell cycle
How Positive Reinforcements Affect my Teaching Practice
Full text linkI completed a self study on how positive reinforcements affect my teaching practice
Estimating the price elasticity of demand for JUUL E-cigarettes among teens
No full textBackground: The widespread popularity of e-cigarettes, particularly JUUL, has led to an alarming increase in teen nicotine use, reversing a 40-year trend. One key question is how sensitive teens’ demand for JUUL is to changes in price. Methods: We estimate the price elasticity of demand using results from an experimental auction where teen nicotine users and nonusers bid on a JUUL kit. Results: We find that a 10 % increase in price leads to as much as a 24 % reduction in JUUL demand among teens using nicotine, and as much as a 45 % reduction among teens not currently using nicotine. The teens in our study were more price sensitive than older adults who took part in a similar earlier study. Conclusions: From a public health standpoint, these are promising results. High e-cigarette taxes may dissuade relatively few older adult cigarette smokers from switching to e-cigarettes, but at the same time be highly effective at preventing teens from becoming e-cigarette users in the first place
Warming increases activity in the common tropical frog Eleutherodactylus coqui
Full text linkTropical ecosystems are expected to experience climate warming, with predicted increases in drying and heat extremes in the coming years. Understanding how these changes will affect terrestrial vertebrates such as amphibians is limited. The Tropical Responses to Altered Climate Experiment in the Luquillo Experimental Forest in northeastern Puerto Rico allows us to study how the tropical forest responds to warming within a replicated plot design. From September 2018 to August 2019, we used mark-recapture sampling to investigate how the spatial population ecology of the common coqui frog (Eleutherodactylus coqui) is impacted by experimentally increasing surface temperatures by 4 °C above ambient. We compared estimates of baseline detection, space use, and the density of frogs in control and warmed plots. Coqui space use and population density did not differ between control and warmed plots. However, coqui detection probabilities were higher in warmed plots, suggesting an increased level of activity relative to individuals in the control (unwarmed) plots. Frog detection increased in all plots with increased precipitation. Our results suggest that, at least in the short-term, the density of an ecological generalist frog like E. coqui does not change as a response to increased surface temperatures. However, short-term responses to warming such as changes in behavior may lead to changes in population dynamics in the long-term. Our research highlights the need to consider mutiple responses in order to understand the effects of climate warming on tropical vertebrates
Against Interiority: Negotiating the Mind and Body in Corinne Manning’s We Had No Rules and Bishakh Som’s Apsara Engine
No full textThe short story has always been about the collective body just by the simple fact that short stories rarely exist alone. And, certainly, the short story has been foundational to any fiction writer from a marginalized group who wants to prove that a readership exists for their work. ... This means-to-an-end fascination with the short story, though, obscures its more important cultural and literary function. It has for the last century been the way—particularly with the privileging of first-person and third-person–limited points of view over omniscience—for a writer from a marginalized group to represent the lives and truths of their larger communities. It’s this second vision of the short story that’s at the core of two recent debut collections: Corinne Manning’s We Had No Rules (Arsenal Pulp Press, 2020) and Bishakh Som’s Apsara Engine (Feminist Press, 2020)
Not Just One, Many: Emasculation, Foreign Impositions, and the Crisis of Masculinity in Máximo Soto Hall’s El problema (1899)
No full textMáximo Soto Hall’s 1899 novel El problema raises several debates: Is this Costa Rica’s first novel, despite the fact that Soto Hall was originally from Guatemala? Is this the first anti-imperialist novel in Latin America? Is this novel anti-imperialist at all, or is it pro-Yankee? ... I propose an alternative reading of the text’s political allegory, one that moves beyond the simplistic parallel between the protagonist Julio’s sexual impotence and the external ideological discourses shaping Costa Rica’s concept of nation. I argue that the crisis of masculinity evident throughout the novel is, rather, a reaffirmation of the institution of marriage, an attempt to regain control over women at a time when modernity was extending freedoms beyond traditional gender roles. The dominant masculinity in the novel, interpreted here as a “masculinidad viril” that demands a departure from, and an oppositional positioning to, all qualities that are considered “feminine” (including Julio’s romantic sensibility), may be understood as a symptom of and reaction to the broader structural weakening of patriarchal society. My specific focus on marriage throughout the novel is twofold: marriage perpetuates women as objects within the economy of symbolic goods and a strategic matrimony likewise ensures the transmission and continuation (via inheritance) of male power and privilege. As such, we observe how dominant notions of masculinity are constantly being renegotiated in tandem with ever-changing social circumstances such as national independence or, in this case, modernization, a process that consequently repositions (male) social subjects and necessitates ideological pivoting in order to perpetuate patriarchal power structures
The Affect of Management in the Classroom
Full text linkThe purpose of this study is to find how types of management effect myself and the classroom on a daily basis. It looks into personal time management and how this will effect the classroom atmosphere and students. Along with time management, I also chose to look at different types of classroom management and which one will work best with the classroom
Effects of Students\u27 Career/Field Experience on Retirement and Career Goals
No full textPast experiences are at the heart of understanding how college students visualize their lives after graduation. Therefore, a student\u27s past experiences will influence their decisions about their future (Haratsis et al., 2015). We are interested in expanding on these ideas to view how college students\u27 past experiences influence their career and retirement goals. In the current study, the relationship between past experiences, career goals, and retirement was measured. The items ask students about past field/job experience, career goals, retirement expectations, and self-efficacy. The Scale of Positive and Negative Experience (SPANE) (Diener et al., 2010) served as our criterion variable. The Future Time Perspective (Koposko & Hershey, 2014) was a statistically significant (p = .043) predictor variable in our study, while the Satisfaction with Life in Retirement Scale (SWLRS) (Gutierrez & Hershey, 2014) also served as a predictor variable for our study but was not significant (p = .344). We determined that an individual who has a better perspective of their future is more likely to be satisfied with their current job. This data suggests that a positive general outlook of the future will impact one’s job satisfaction. From an applied perspective, students should be encouraged to foster a positive outlook toward career aspirations in order to create a stable perception of the future. Additionally, our research supports the development of university-level career development and retirement education programs to create a foundation for students beyond matriculation
The Effects of Procrastination on Teaching Practice
Full text linkThis project examined the effects that procrastination can have on teaching practices as well as gathering data on specific reasons for procrastination as it relates to the teaching profession. Procrastination is an issue that affects everyone, whether it be a positive effect or a negative effect. The purpose of this self-study sought to answer the question of how procrastination affects my performance as a teacher specifically and address the reasons behind it to lessen the likelihood of procrastination occurring