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    Low flow: Selecting a limited flow cytometry panel where resources are constrained

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    Diagnosis and treatment of patients with haematological malignancies (HM) is hampered by access to pathology services in resource-limited settings (RLS). Internationally accepted guidelines and diagnostic criteria for HM require access to sophisticated analysis including comprehensive flow cytometry (FCM) for minimum essential diagnosis and treatment, which is technically challenging in RLS. This review will define these shortcomings and examine the use of limited FCM panels in RLS. While a consensus guideline exists for a limited chronic lymphocytic leukaemia (CLL) FCM panel, this has yet to be validated in a large cohort. Currently, there are no consensus-based and resource-stratified diagnostic protocols defining limited FCM panels for the diagnosis of acute leukaemia where resources are limited. There is an unmet need for such guidelines, supported by evidence, for the diagnosis of the most common HM. This systematic review defines consensus-based limited FCM panels from the literature that may be used in the interim

    Honouring the Legitimacy and Power of Shared Lived Experience in Social Work

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    [Extract] Lived experience is an invaluable source of knowledge in social work. Service users, mental health lived experience experts, Indigenous, feminist, and disability practitioners, researchers, and scholars and Mad scholarship all have emphasised the importance of listening to lived experience (see Cantley, Citation2025; Prehn, Citation2025; Woodlock et al. Citation2025; this Issue; Sinclair & Mahboub, Citation2024). Similarly, patient-centred, trauma-informed, and culturally responsive practice approaches have helped us to invert notions of expert knowledge and to recognise and uphold the richness, power, legitimacy, and validity of lived experience expertise (Loughhead et al., Citation2023; Sinclair & Mahboub, Citation2024)

    Crafting an organizational strategy for the new era: a qualitative study of artificial intelligence transformation in a homegrown Singaporean hotel chain

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    Purpose This study discusses the strategic integration of artificial intelligence (AI) within the hospitality industry by examining the experience of a locally owned Singaporean hotel chain. It will address a central gap in the prior research’s lack of attention to employees’ perspective of AI adoption in domestic hotel chains. Design/methodology/approach Using the grounded theory approach, this study used a thematic analysis of in-depth interviews with ten managers of a locally owned hotel chain who plan to implement AI in their chain. Findings The results show that while AI offers many advantages, including lower costs, more effectiveness, and better customer experience, human intervention is still necessary to provide individualized and personalized attention and service. The study emphasizes the necessity of a well-rounded strategy that uses AI’s potential without sacrificing the crucial human element that characterizes the best hospitality experience. Research limitations/implications Future research should address this study’s limitations using larger, diverse samples and mixed methods to explore AI adoption’s impact on hospitality. Practical implications Leaders in the hospitality industry should foster an organizational culture emphasizing empowerment and continuous learning to integrate AI technologies successfully. The insights from a Singaporean hotel chain suggest that AI adoption can enhance employee and customer experiences. However, effective AI strategies require considering cultural and organizational differences and communicating their benefits. Aligning AI implementation with customer preferences, such as offering tech-driven solutions for younger, tech-savvy guests while maintaining personal interaction for less IT-savvy customers, is key to maintaining branding. This strategic, differentiated approach ensures AI enhances operational efficiency and maximizes guest satisfaction through tailored, personalized services. Originality/value This study is unique in its focus on the employees’ perspective of AI adoption within a domestic hotel chain in Singapore, a viewpoint that has been largely overlooked in previous research. By employing a grounded theory approach and conducting in-depth interviews with managers, the study provides rich, qualitative insights into the practical challenges and benefits of integrating AI in the hospitality sector. This approach highlights the operational advantages of AI and underscores the indispensable role of human interaction in delivering personalized and high-quality service, thus offering a balanced view of AI’s impact on the industry

    Transcriptomic changes during induction of tetrasporogenesis in the red seaweed Asparagopsis armata

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    The red seaweed Asparagopsis armata, recognised for its ability to reduce methane emissions from enteric fermentation when incorporated as a ruminant feed additive, is a key target for aquaculture development. However, despite advancements in this field, there remains a critical gap in understanding the internal mechanisms governing reproduction in A. armata. In this study, we examined transcriptomic changes throughout the induction of tetrasporogenesis, with a primary focus on metabolism, and specifically on genes and pathways related to plant growth regulators and environmental signal processing. Considerable variability in gene expression was observed leading up to and during the initiation of tetrasporogenesis. Notably, genes associated with polyamine metabolism, particularly the candidate ornithine decarboxylase (ODC) gene previously linked to seaweed reproduction, were highly upregulated leading up to tetrasporogenesis. Enriched pathways related to environmental signalling were identified, including the phosphatidylinositol signalling system and associated phospholipase C enzymes, along with marked upregulation of genes encoding membrane-associated photoreceptors. Pathways associated with carbon metabolism and steroid hormone production also showed significant enrichment throughout the induction of tetrasporogenesis, although a significant portion of the genes involved in these pathways have unknown function. These findings collectively offer a detailed overview of the processes underlying tetrasporogenesis in A. armata, facilitating further targeted investigations into the internal regulation of seaweed reproductive biology

    Learning to thrive through autonomy: a thematic analysis of the role of recalled parental autonomy in the transition to motherhood

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    Becoming a mother requires the continual redefinition of current self-identity. This can be challenging for women who struggle being autonomous due to unfulfilled core emotional needs from early interactions with primary caregivers. Existing literature on the impact of early experiences on motherhood is largely quantitative and focuses on adverse childhood experiences and broad parenting constructs. Informed by the findings of our quantitative study (Leong et al., 2024), this secondary predominately qualitative study explored primiparous mothers’ perceptions of both adverse and positive experiences of parental autonomy, and how they draw upon those experiences in early motherhood. The sample comprised 15 first-time mothers in their first-year post-birth, aged 18–36, recruited in Singapore from a large mixed-method study which examined the impact of early experiences of being parented on postnatal mental health. Referencing specific interactions on the Positive Parenting Schema Inventory (PPSI; Louis et al., 2020a) and the Young Parenting Inventory Revised (YPI-R2; Louis et al., 2018), semi-structured interviews were conducted with the mothers on their perceptions of parental autonomy and how those experiences impacted their parenting style, sense of competence and postnatal mental health. Interview transcripts were analysed drawing upon guidelines and principles of reflexive thematic analysis. Three broad themes were generated; (i) making sense of autonomy; (ii) learning to thrive through autonomy; and (iii) striving for balance through autonomy. The findings highlight the instrumental role of parental autonomy and culture in the transition to motherhood and inform the design and implementation of postnatal mental health services, parenting programmes and future research

    Prevalence and Correlates of (Internet) Gaming Disorder among Young Adults in Singapore

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    There is a lack of estimates of prevalence rates of Internet gaming disorder (IGD) and gaming disorder (GD) among young adults in Singapore. Consequently, the current study aimed to examine the prevalence rates of IGD and GD, and their relationships with known correlates. Participants were a representative sample of 1008 young adults in Singapore. They completed instruments that assess IGD, GD, gaming motivations, negative emotional states, and sleep quality. The results showed a prevalence rate of 10.3% for IGD and 5.0% for GD. Males had a higher IGD and GD prevalence rate (14.6% and 7.2%) than females (6.2% and 2.8%). Furthermore, participants with IGD or GD reported higher gaming time, and higher scores on gaming motivations, negative emotional states, and sleep quality than their counterparts with no IGD or GD. The higher prevalence rates in this study compared to other global studies suggest that problematic gaming is a social issue in Singapore. Known correlates relating to gender, gaming time, motivations, and emotions were confirmed in the current study. The finding that participants with IGD or GD had better sleep quality was inconsistent with previous studies and could be due to the use of a single item to assess sleep quality. Limitations include the use of a cross-sectional correlational design. Future research could develop and evaluate prevention programs or intervention programs to alleviate the symptoms and negative consequences of problematic gaming

    Photo-id as an alternative to monitor marine turtles in the Gulf of Venezuela

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    Conservation management programs have used diverse methods to monitor populations of threatened species that vary in effectiveness, duration, and costs; making its implementation a challenge. The present study was carried out to test the use of photo-identification as an economical and efficient alternative for marine turtle monitoring in the Gulf of Venezuela. The implementation of this protocol is possible due to the unique and unrepeatable facial scales pattern of individuals in the marine turtles. We created a database of photo-identifiable profiles available from records of turtles captured, tagged, and released in the Gulf of Venezuela from 2000 to 2017 (n = 118). Likewise, we used two photo-matching software (I3S Pattern and Nature Pattern Match) to optimize the process of compatibility of individuals and we evaluated their efficiency in comparison with the non-assisted manual method (“by human eye” or "by naked eye"). We found that I3S Pattern was more effective during the matching process than NPM (90% and 65% accuracy respectively), while the manual method was much more accurate than the software. However, the former method is impractical when working with large databases. Our results indicate that I3S Pattern represents the most efficient software of image matching by reducing the time needed and simplifying the manual “by human eye” analysis. We recommend incorporating more photos in the database in order to verify the effectiveness of both studied software, and regularly to corroborate the results generated by the software assessed on this research using the “human eye” manual method

    Situating Narratives of Decline: Surveying the Literature of Crisis from a Regional Humanities Student Perspective

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    [Extract] As first-in-family university graduates from regional northern and far-northern Queensland, both of us have found the work of this essay to be a challenging—and upsetting—exercise. We care deeply for the regional university which has provided us with educations in the humanities, but the feelings of anger, disappointment, and despondency we endure when considering the future of our disciplines at regional institutions such as the one we attended are difficult to translate into clear-headed academic prose. Our stories are different, but our lived experiences demonstrate what will be—and has already been—lost when regional Bachelor of Arts programs are repeatedly restructured, reoriented, refocused, refreshed, or cut entirely. Jade was born in Cairns, in the far-north of Queensland, but was raised in the smaller, regional town of Innisfail. Her father had left high school after Year 10 to pursue employment and Jade’s mother had been unable to continue schooling at the age of fourteen. Despite the interruption, Jade’s mother had taken a less-conventional pathway to completing her high school education later in life and subsequently worked her way up into professional roles. Straight out of school, Jade moved south to Townsville to start a double degree in arts and science. She struggled to adjust to university studies and found herself failing subjects for the first time in her life. Away from home and dealing with difficult personal circumstances in addition to the challenges of university, Jade returned home to Innisfail. In 2021 she decided to follow her passion for literature and transitioned into a Bachelor of Arts with a major in English, remaining enrolled at the same regional university but now studying externally. Settling into her work, Jade gained momentum. Maintaining good grades and finally having an opportunity to network with like-minded people, she even had the chance to co-author a paper with a lecturer and fellow classmates as an undergraduate. Earlier this year at her graduation, she was recognised for achieving the highest aggregate result for first-year English literature

    Methane and carbon dioxide production and emission pathways in the belowground and draining water bodies of a tropical peatland plantation forest

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    Peatlands are crucial yet vulnerable carbon stores. Here, we investigated carbon biogeochemical processes in tropical peatlands converted to plantations. We measured carbon dioxide (CO2) and methane (CH4) concentrations, stable isotope ratios and radiocarbon content in an experimental Acacia crassicarpa plantation in Sumatra, Indonesia. We found exceptionally high levels of dissolved organic carbon (DOC), CO2, and CH4 in porewater and drainage networks, indicating that Acacia plantations are carbon hotspots due to their high productivity and exposed carbon‐dense substrates. Stable isotope models revealed that while CO2 and CH4 are produced belowground, CH4 contribution was lower than in natural undrained peatlands. Radiocarbon analysis suggested that remobilized carbon contributed to the carbon pool, with a median age of ∼470 years before present. These findings constrain the links between land‐use, water table levels, and carbon dynamics, with implications for carbon management in plantation peatlands

    The Evolving Tourism Marketplace: Changing Tourist Shopping Markets

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    Global mass tourism is a relatively recent phenomenon emerging at the start of the twenty first century with the rise of budget airlines, aircraft capable of longer flights with larger capacities, globalization and decreased travel restrictions and the enthusiasm of Chinese consumers to take up opportunities for international travel (Buckley et al., 2015). While tourism has a much longer history the scale and diversity of truly global mass tourism is limited to the last 30 years. Although this is not a long time in any historical context it does mean that at a large proportion of the world’s human population have never lived without global tourism. Not surprisingly then a question asked at a tourism academic conference in 2019 about imagining a world without international tourism resulted in an extensive pause and much consternation. Less than 6 months after this question was posed the onset of the COVID-19 pandemic essentially shut down international travel and much domestic travel. This resulted in an avalanche of academic predictions on the future of tourism, many of which have not yet been subjected to any critical analysis. These discussions fell into two main categories- once the pandemic was under control tourism would/should return to its pre-pandemic levels and quickly grow beyond those, or tourism would/should return but in a much-changed form (Higgins-Desbiolles, 2020). While to date neither of these two extremes appear to be correct, on closer inspection with more critical analysis, changing trends and emerging forces for change can be identified. This chapter seeks to explore these changing trends and emerging forces and their impact on tourist shopping markets. For the purposes of this chapter tourist shopping will be defined “as a recreational activity in which tourists browse, select and purchase goods to take home during their travel” (Jin et al., 2017, p. 121). This definition includes people who take shopping focussed tours/trips as well as tourists who buy personal items and souvenirs as part of a trip. There is considerable overlap between leisure shopping and tourism. Both are hedonic, recreational activities that involve discretionary expenditure (Backstrom, 2011). For some leisure shopping is a prime feature of tourist activities and for others the two phenomena are substitutes for each other. After outlining key trends and forces in both the retail shopping and tourism systems, the chapter will use social practice theory from sociology as the basis for identifying and analysing changing and emerging tourist shopping markets It will conclude the analysis with some initial implications for both research into tourist shopping and for tourism practitioners

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