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Investigating a Family of Fungal Pseudopeptidases with Bioinformatics Tools
Get PDFEnzymes catalyze chemical reactions; however, some proteins related to enzymes lack enzyme activity. To investigate changes that occur as enzymes becomes pseudoenzymes, I studied a unique family of fungal carboxypeptidases. I identified all fungal carboxypeptidase protein sequences in the NCBI database, constructed an alignment and phylogenetic tree for sequence comparison, and used Alphafold2 for 3D modeling of representative proteins. Fourteen unique groups were identified. All fungal proteins selected are separated into 3 categories, predicted to be active, intermediate, and inactive, respectively. Proteins predicted to be pseudoenzymes contained additional loops in mutation hotspots not found in predicted active enzymes. This research integrates modern bioinformatics tools for analyzing enzyme function and contributes to understanding the evolution of enzymes
Understanding the Mechanism Behind HFIP\u27s Effect on Diastereomeric Separation of Oligonucleotides
Get PDFThe goal of this research was to determine the mechanism behind 1,1,1,3,3,3-hexafluoroisopropanol\u27s (HFIP0 ability to suppress the separation of oligonucleotide diastereomers. This was accomplished by selecting different molecules that were like HFIP but differed in specific ways such as polarity, number and type of halogen(s), alcohol group location, carbon chain length, hydrophobicity, and chao/kosmotropicity. These molecules replaced HFIP in an IPRP chromatography method to determine if splitting of the diastereomers was observed and then they would becompared to HFIP\u27s effect. Viscosity tests were to run to acquire quantitative data to determine chao/kosmotropicity. No decisive trend among a single factor was observed meaning that more than one factor effects diastereomer selectivity.
Note that this research is qualitative in nature and has no statistical analysis
Healing the Trauma: Toward a Missiology for Contemporary Russia
Get PDFThis article addresses the trauma of Stalinism from the standpoint of a missiological reading of Luke 4:16-21. This programmatic sermon of Jesus at Nazareth, whose meaning is unfolded in the larger context of Luke-Acts, is of primary importance for the current Russian culture, which is suffering from the consequences of a time when its culture was warped by the forces of the demonic realm. The rationale for using Luke-Acts in a post-Soviet theology of mission and the cluster of theological theses that underlie Jesus’ program of liberation are both foundational for a missiological paradigm intended for post-totalitarian Russian society
Ruqyah Shariah: An Analytical Exploration
Get PDFRuqyah Shariah—Law-Compliant Exorcism or Islamic Healing Prayer— is a spiritual and physical remedy that involves reciting qur’anic verses and authentic supplications. Rooted deeply in Islamic tradition, it offers a wholistic approach to healing and protection. Ruqyah has been a practice since the early days of Islam, with numerous hadiths documenting its use by Muhammad and his companions.
This article seeks to explore the multifaceted aspects of ruqyah shariah, by examining its legal foundations, practical guidelines, and ethical implications, while seeking to provide a well-rounded perspective on its significance and application in the modern context
Pastoral Burnout: Causes, Consequences and Closure
Get PDFPastoral burnout is a growing concern among religious leaders in North America, with significant consequences for both individuals and congregations. No denomination is unaffected by its debilitating presence; even Seventh-day Adventist clergy are not immune to this increasing stress-related twenty-first century health malady. Several factors contribute to pastoral burnout, including excessive workload, unrealistic expectations, lack of support, and boundary violations. These aspects often lead to emotional exhaustion, depersonalization, reduced personal accomplishment, and though rare, suicide. This presentation will explore the causes, consequences, and suggested conclusion with potential strategies for intervention and prevention of pastoral burnout
Spiritual Formation for the Global Church: A Multi-Denominational, Multi-Ethnic Approach [review] / Brandt, Ryan A. and John Frederick, eds.
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Aramaic: A History of the First World Language [review] / Gzella, Holger.
Get PDFThis is a book revie
Wisdom From the Psalms
No full textClick here to download the audio file.
Here is the Study Guide for the week ending February 24, 2023.
This week’s discussion on our YouTube channe