5430 research outputs found
Sort by
Apparatus used by Priestley for the study of gases
No full textApparatus used by Priestley for the study of gases. From Priestley\u27s Experiments and Observations on Different Kinds of Air, 1774https://digitalcommons.rockefeller.edu/alfred-cohn-collection/1039/thumbnail.jp
Stockholm and beyond
No full textPhoto by Lubosh Stepanekhttps://digitalcommons.rockefeller.edu/blobel-molecular-biology/1043/thumbnail.jp
Günter Blobel, 2003
No full textGünter Blobel in his laboratory, 2003
Phoby by Lubosh Stepanekhttps://digitalcommons.rockefeller.edu/blobel-molecular-biology/1049/thumbnail.jp
The ruins of the Church of Our Lady in Dresden
No full textThe ruins of the Church of Our Lady in Dresden, circa 1960shttps://digitalcommons.rockefeller.edu/blobel-molecular-biology/1053/thumbnail.jp
Save Venice Inc.
No full textAs members of Save Venice, Laura Maioglio Blobel (Honorary Director) and Günter Blobel attended the biannual Summertime in Venice Gala, 2012
Save Venice Inc. is a nonprofit organization based in New York with a mission to raise funds to protect the cultural patrimony of the city of Venice. Every year, the Board of Save Venice, selects restoration projects in collaboration with the Italian Ministry of Culture based on artistic merit, historical importance, and urgency of need. Save Venice has numerous restorations underway throughout the city, made possible with support from individuals, foundations, and corporations who believe in a universal responsibility to preserve the world’s irreplaceable artistic and cultural treasures found in Venice.
Photo by Lubosh Stepanekhttps://digitalcommons.rockefeller.edu/blobel-molecular-biology/1061/thumbnail.jp
Siddhartha Mukherjee: A Biography of the Gene
No full textMarkus Library Book Talk 2018
A Biography of the Gene
Siddhartha Mukherjee
Posted with permissionhttps://digitalcommons.rockefeller.edu/book_talk_sid_mukherjee/1000/thumbnail.jp
Marjorie McCarty Oral History. Part 2: The Rockefeller Institute in 1957
No full textInterview recorded on September 14th, 2017. Part of the Rita and Frits Markus Library Oral History project.https://digitalcommons.rockefeller.edu/marjorie-mccarty/1001/thumbnail.jp
Identification and Characterization of Hells-CDCA7, a Nucleosome Remodeling Complex Required for Mitotic Chromosome Structure
Get PDFThe major components of mitotic chromosomes including histones, topoisomerase II and condensin are known to compact and shape the chromatin into rod-like chromatids. However, a complete picture of the proteins involved in shaping mitotic chromatin is unsettled. Here, I perform mass spectrometry on chromatin isolated from Xenopus egg extracts. By comparing interphase and M phase, I reveal how the chromatin proteome is affected by the cell cycle. I find that although topoisomerase II associates with chromatin throughout the cell cycle, its catalytic activity is greatly enhanced in mitosis where it can act on nucleosomal substrates. In contrast, condensin is specifically recruited to M phase chromatin and prefers a non-nucleosomal substrate. I show that nearly all proteins involved in nucleosome assembly and remodeling are evicted from mitotic chromosomes, concurrent with a reduction in mitotic nucleosome assembly and discuss a role for these phenomena in shaping mitotic chromosomes. In analyzing my mass spectrometry data, I noticed that subunits of stoichiometric chromatin-bound complexes behaved similarly across a variety of conditions. Using this principle, I identify a novel nucleosome remodeling complex comprising HELLS and CDCA7, two proteins known to be causative for Immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome, a rare immunodeficiency disease. Consistent with previous literature, HELLS alone fails to remodel nucleosomes, but the HELLS-CDCA7 complex possesses robust nucleosome remodeling activity. CDCA7 is essential for loading HELLS onto chromatin, and CDCA7 harboring patient ICF mutations fails to recruit the complex to chromatin. Finally, I show that the HELLSCDCA7 complex is required for proper mitotic chromosome structure. Together, my study identifies a unique bipartite nucleosome remodeling complex where the functional remodeling activity is split between two proteins. These data lead me to a unifying model for ICF syndrome where all known ICF mutations converge on defective DNA methylation. I propose that HELLS-CDCA7 mediated remodeling of juxtacentromere heterochromatic nucleosomes facilitates DNMT3B-mediated methylation and discuss this model alongside alternative models for ICF syndrome. Finally, I consider the role of CDCA7 paralogs in differentially regulating HELLS, and present an outlook on the future of ICF syndrome research
Marjorie McCarty Oral History. Part 6 (a): My husband - Maclyn McCarty
No full textInterview recorded on September 14th, 2017. Part of the Rita and Frits Markus Library Oral History project.https://digitalcommons.rockefeller.edu/marjorie-mccarty/1005/thumbnail.jp
From the collection of Alfred Cohn
No full textThe exhibit From the Collection of Alfred Einstein Cohn was on view at Level A on September 27-28 during the Markus Library Open House 2018
Idea, design: Olga Nilova, Outreach Librarian
Photo by Lubosh Stepanekhttps://digitalcommons.rockefeller.edu/alfred-cohn-collection/1000/thumbnail.jp