5430 research outputs found
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Pathogenesis
No full textSlide 3-6: Pathogenesis; Protectionhttps://digitalcommons.rockefeller.edu/immunodeficiency-disease/1005/thumbnail.jp
Leonia Bozzaco, Christine Trumpfheller, and Angela Granelli-Piperno
No full textSlide 4-22: Leonia Bozzaco( left), Christine Trumpfheller (right), and Angela Granelli-Piperno (center), n.d.https://digitalcommons.rockefeller.edu/endocytosis/1020/thumbnail.jp
B16/GalCer Tumor Cells
No full textSlide 5-29: B16/GelCer tumor cells induce long-lived tumor challengehttps://digitalcommons.rockefeller.edu/nkt-cells/1027/thumbnail.jp
Molecular Characterization of Mammalian De Novo DNA Methyltransferase Chromatin Recruitment
Get PDFPrecise deposition of CpG methylation is critical for mammalian development and tissue homeostasis and is often dysregulated in human diseases. The genomic localization of de novo DNA methyltransferases 3A (DNMT3A) and 3B (DNMT3B) is facilitated in part by chromatin reader domains to establish DNA methylation patterns genome-wide. Recent work has highlighted a role for the PWWP reader domain in directing recruitment of DNMT3B to actively transcribed gene bodies. However, our understanding of de novo DNA methyltransferase chromatin recruitment remains incomplete. Here I demonstrate using a variety of biochemical and cellular techniques that DNMT3A genomic localization is regulated by competing chromatin recruitment pathways. Under steady-state conditions, DNMT3A is targeted to non-coding intergenic regions through PWWP reader domain recognition of H3K36me2, an abundant histone post-translational modification (PTM) in cells. Following depletion of cellular H3K36me2 levels, DNMT3A is re-targeted to actively transcribed gene bodies through PWWP domain interaction with H3K36me3, thereby resembling the genomic localization of DNMT3B. Lastly, loss of PWWP domain reader functionality reveals the presence of a previously uncharacterized chromatin reader domain within DNMT3A that serves as an alternative targeting mechanism to facilitate de novo methylation of Polycomb-regulated regions, including a subset of CpG islands. Perturbations to these recruitment mechanisms may underlie the pathology of developmental overgrowth and undergrowth syndromes associated with distinct germline mutations in DNMT3A and may also contribute to the altered DNA methylation landscapes observed in diverse cancers
Brentano String Quartet
No full textBrentano String Quartet performed Mendelssohn: String Quartet No. 6 in F Minor, Op. 80; Beethoven String Quartet No. 12 in E-flat Major, Op. 127.
Photo by Jürgen Frankhttps://digitalcommons.rockefeller.edu/tri-institutional-noon-recitals/1000/thumbnail.jp
Yi-Nuo Wang, Piano
No full textYi-Nuo Wang, piano, performed Béla Bartók (1881-1945): Piano Sonata, Sz. 80; Johannes Brahms (1833-1897): 8 Klavierstücke, Op. 76; Chen Yi (b. 1953): Ba Ban; Sergei Rachmaninoff (1873-1943): Etude in D Minor, Op. 39, No. 8; Etude in D Major Op. 39, No. 9https://digitalcommons.rockefeller.edu/tri-institutional-noon-recitals/1003/thumbnail.jp
Quartetto di Cremona
No full text2019, November 1
Quartetto di Cremona, string quartet: Cristiano Gualco and Paolo Andreoli, violins; Simone Gramaglia, viola; Giovanni Scaglione, cello performed Verdi: String Quartet in E Minor; Beethoven: String Quartet No. 14 in C-sharp Minor, Op.131https://digitalcommons.rockefeller.edu/tri-institutional-noon-recitals/1018/thumbnail.jp
Jimmy Roberts, Piano
No full text2019, January 4
Jimmy Roberts, award-winning pianist and composer for the musical theater (“I Love You, You’re Perfect, Now Change”): Songs for the New Year. Plays and sings music that touches on the many moods of this transition to 2019. Revel in the wit and sentiment of his original songs, plus unforgettable melodies of Bernstein, Sondheim, and Rodgers—with a dash of Beethoven and Bach!https://digitalcommons.rockefeller.edu/tri-institutional-noon-recitals/1047/thumbnail.jp
Sarah J. Schlesinger Oral History. Part 5: Getting M.D.
No full textInterview recorded in spring 2019. Part of The Rita and Frits Markus Library Oral History projecthttps://digitalcommons.rockefeller.edu/sarah-schlesinger/1004/thumbnail.jp
Tile fragment
No full textTile fragment decorated with the bud of a flower; earthenware, blue and white glaze. Persia, 18th Century, Mirsky Collection
Courtesy of the Rockefeller Archive Center
Photo by Lubosh Stepanekhttps://digitalcommons.rockefeller.edu/the-evolving-campus/1047/thumbnail.jp